This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 165.16 s (31 us/read; 1.94 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 68,320 (1.3%) Reads that were too short: 26,009 (0.5%) Reads written (passing filters): 42,311 (0.8%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 7,830,646 bp (0.5%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 68320 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 41 1302.2 0 41 7 415 1302.2 0 415 8 1059 1302.2 0 1059 9 3489 1302.2 0 3489 10 26 1302.2 0 26 11 21 1302.2 0 21 12 12 1302.2 0 12 13 19 1302.2 0 19 14 20 1302.2 0 20 15 20 1302.2 0 20 16 13 1302.2 0 13 17 17 1302.2 0 17 18 94 1302.2 0 94 19 71 1302.2 0 71 20 15 1302.2 0 15 21 15 1302.2 0 15 22 20 1302.2 0 20 23 35 1302.2 0 35 24 20 1302.2 0 20 25 18 1302.2 0 18 26 9 1302.2 0 9 27 16 1302.2 0 16 28 10 1302.2 0 10 29 23 1302.2 0 23 30 21 1302.2 0 21 31 31 1302.2 0 31 32 29 1302.2 0 29 33 26 1302.2 0 26 34 25 1302.2 0 25 35 15 1302.2 0 15 36 22 1302.2 0 22 37 28 1302.2 0 28 38 33 1302.2 0 33 39 19 1302.2 0 19 40 19 1302.2 0 19 41 23 1302.2 0 23 42 60 1302.2 0 60 43 28 1302.2 0 28 44 29 1302.2 0 29 45 24 1302.2 0 24 46 20 1302.2 0 20 47 26 1302.2 0 26 48 19 1302.2 0 19 49 29 1302.2 0 29 50 15 1302.2 0 15 51 46 1302.2 0 46 52 55 1302.2 0 55 53 12 1302.2 0 12 54 24 1302.2 0 24 55 18 1302.2 0 18 56 24 1302.2 0 24 57 325 1302.2 0 325 58 176 1302.2 0 176 59 25 1302.2 0 25 60 22 1302.2 0 22 61 24 1302.2 0 24 62 19 1302.2 0 19 63 18 1302.2 0 18 64 20 1302.2 0 20 65 17 1302.2 0 17 66 25 1302.2 0 25 67 25 1302.2 0 25 68 29 1302.2 0 29 69 23 1302.2 0 23 70 22 1302.2 0 22 71 22 1302.2 0 22 72 25 1302.2 0 25 73 30 1302.2 0 30 74 129 1302.2 0 129 75 72 1302.2 0 72 76 304 1302.2 0 304 77 24 1302.2 0 24 78 101 1302.2 0 101 79 183 1302.2 0 183 80 271 1302.2 0 271 81 57 1302.2 0 57 82 211 1302.2 0 211 83 134 1302.2 0 134 84 69 1302.2 0 69 85 358 1302.2 0 358 86 171 1302.2 0 171 87 106 1302.2 0 106 88 89 1302.2 0 89 89 188 1302.2 0 188 90 60 1302.2 0 60 91 234 1302.2 0 234 92 125 1302.2 0 125 93 154 1302.2 0 154 94 405 1302.2 0 405 95 1181 1302.2 0 1181 96 281 1302.2 0 281 97 1254 1302.2 0 1254 98 687 1302.2 0 687 99 775 1302.2 0 775 100 516 1302.2 0 516 101 146 1302.2 0 146 102 195 1302.2 0 195 103 47 1302.2 0 47 104 34 1302.2 0 34 105 45 1302.2 0 45 106 90 1302.2 0 90 107 87 1302.2 0 87 108 96 1302.2 0 96 109 183 1302.2 0 183 110 117 1302.2 0 117 111 386 1302.2 0 386 112 6963 1302.2 0 6963 113 426 1302.2 0 426 114 70 1302.2 0 70 115 104 1302.2 0 104 116 174 1302.2 0 174 117 179 1302.2 0 179 118 52 1302.2 0 52 119 30 1302.2 0 30 120 181 1302.2 0 181 121 158 1302.2 0 158 122 98 1302.2 0 98 123 108 1302.2 0 108 124 51 1302.2 0 51 125 94 1302.2 0 94 126 117 1302.2 0 117 127 135 1302.2 0 135 128 112 1302.2 0 112 129 125 1302.2 0 125 130 175 1302.2 0 175 131 180 1302.2 0 180 132 226 1302.2 0 226 133 296 1302.2 0 296 134 438 1302.2 0 438 135 236 1302.2 0 236 136 247 1302.2 0 247 137 159 1302.2 0 159 138 134 1302.2 0 134 139 105 1302.2 0 105 140 294 1302.2 0 294 141 136 1302.2 0 136 142 121 1302.2 0 121 143 60 1302.2 0 60 144 103 1302.2 0 103 145 108 1302.2 0 108 146 270 1302.2 0 270 147 215 1302.2 0 215 148 176 1302.2 0 176 149 145 1302.2 0 145 150 112 1302.2 0 112 151 133 1302.2 0 133 152 49 1302.2 0 49 153 60 1302.2 0 60 154 123 1302.2 0 123 155 149 1302.2 0 149 156 407 1302.2 0 407 157 175 1302.2 0 175 158 155 1302.2 0 155 159 156 1302.2 0 156 160 148 1302.2 0 148 161 137 1302.2 0 137 162 137 1302.2 0 137 163 103 1302.2 0 103 164 150 1302.2 0 150 165 188 1302.2 0 188 166 379 1302.2 0 379 167 320 1302.2 0 320 168 377 1302.2 0 377 169 164 1302.2 0 164 170 87 1302.2 0 87 171 87 1302.2 0 87 172 75 1302.2 0 75 173 224 1302.2 0 224 174 250 1302.2 0 250 175 316 1302.2 0 316 176 206 1302.2 0 206 177 57 1302.2 0 57 178 58 1302.2 0 58 179 239 1302.2 0 239 180 236 1302.2 0 236 181 487 1302.2 0 487 182 288 1302.2 0 288 183 183 1302.2 0 183 184 182 1302.2 0 182 185 355 1302.2 0 355 186 465 1302.2 0 465 187 210 1302.2 0 210 188 59 1302.2 0 59 189 52 1302.2 0 52 190 63 1302.2 0 63 191 113 1302.2 0 113 192 217 1302.2 0 217 193 849 1302.2 0 849 194 858 1302.2 0 858 195 628 1302.2 0 628 196 511 1302.2 0 511 197 236 1302.2 0 236 198 145 1302.2 0 145 199 366 1302.2 0 366 200 430 1302.2 0 430 201 526 1302.2 0 526 202 357 1302.2 0 357 203 145 1302.2 0 145 204 166 1302.2 0 166 205 303 1302.2 0 303 206 277 1302.2 0 277 207 273 1302.2 0 273 208 228 1302.2 0 228 209 158 1302.2 0 158 210 174 1302.2 0 174 211 289 1302.2 0 289 212 357 1302.2 0 357 213 164 1302.2 0 164 214 155 1302.2 0 155 215 206 1302.2 0 206 216 301 1302.2 0 301 217 347 1302.2 0 347 218 256 1302.2 0 256 219 293 1302.2 0 293 220 556 1302.2 0 556 221 685 1302.2 0 685 222 1122 1302.2 0 1122 223 164 1302.2 0 164 224 115 1302.2 0 115 225 239 1302.2 0 239 226 240 1302.2 0 240 227 335 1302.2 0 335 228 415 1302.2 0 415 229 240 1302.2 0 240 230 132 1302.2 0 132 231 115 1302.2 0 115 232 86 1302.2 0 86 233 88 1302.2 0 88 234 101 1302.2 0 101 235 170 1302.2 0 170 236 187 1302.2 0 187 237 183 1302.2 0 183 238 113 1302.2 0 113 239 210 1302.2 0 210 240 83 1302.2 0 83 241 91 1302.2 0 91 242 114 1302.2 0 114 243 299 1302.2 0 299 244 257 1302.2 0 257 245 259 1302.2 0 259 246 317 1302.2 0 317 247 282 1302.2 0 282 248 129 1302.2 0 129 249 79 1302.2 0 79 250 51 1302.2 0 51 251 78 1302.2 0 78 252 123 1302.2 0 123 253 137 1302.2 0 137 254 373 1302.2 0 373 255 932 1302.2 0 932 256 1485 1302.2 0 1485 257 405 1302.2 0 405 258 334 1302.2 0 334 259 237 1302.2 0 237 260 102 1302.2 0 102 261 78 1302.2 0 78 262 315 1302.2 0 315 263 1720 1302.2 0 1720 264 1914 1302.2 0 1914 265 158 1302.2 0 158 266 420 1302.2 0 420 267 366 1302.2 0 366 268 323 1302.2 0 323 269 191 1302.2 0 191 270 402 1302.2 0 402 271 140 1302.2 0 140 272 167 1302.2 0 167 273 167 1302.2 0 167 274 127 1302.2 0 127 275 213 1302.2 0 213 276 155 1302.2 0 155 277 44 1302.2 0 44 278 59 1302.2 0 59 279 151 1302.2 0 151 280 230 1302.2 0 230 281 178 1302.2 0 178 282 109 1302.2 0 109 283 84 1302.2 0 84 284 62 1302.2 0 62 285 94 1302.2 0 94 286 171 1302.2 0 171 287 219 1302.2 0 219 288 79 1302.2 0 79 289 44 1302.2 0 44 290 49 1302.2 0 49 291 119 1302.2 0 119 292 164 1302.2 0 164 293 182 1302.2 0 182 294 169 1302.2 0 169 295 179 1302.2 0 179 296 76 1302.2 0 76 297 64 1302.2 0 64 298 93 1302.2 0 93 299 207 1302.2 0 207 300 174 1302.2 0 174 301 41 1302.2 0 41 Finished in 1.73 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 42,311 Reads with adapters: 1,117 (2.6%) Reads that were too short: 128 (0.3%) Reads written (passing filters): 989 (2.3%) Total basepairs processed: 7,830,646 bp Total written (filtered): 264,264 bp (3.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1117 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 12 0.0 3 0 5 1 6 17 880 0.0 3 853 25 2 18 24 0.0 3 1 1 21 1 19 19 0.0 3 1 2 4 12 21 1 0.0 3 1 23 3 0.0 3 0 0 0 3 32 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 75 7 0.0 3 0 0 0 7 76 23 0.0 3 0 0 0 23 78 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 93 3 0.0 3 0 0 0 3 95 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 187 45 0.0 3 0 0 0 45 188 59 0.0 3 0 0 0 59 189 7 0.0 3 0 0 2 5 191 2 0.0 3 0 0 1 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.04 s (40 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 989 Reads with adapters: 26 (2.6%) Reads written (passing filters): 989 (100.0%) Total basepairs processed: 264,264 bp Total written (filtered): 264,180 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 26 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 15.4% C: 7.7% G: 38.5% T: 38.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 20 15.5 0 20 4 6 3.9 0 6