This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 169.96 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 142,776 (2.7%) Reads that were too short: 14,900 (0.3%) Reads written (passing filters): 127,876 (2.4%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 27,664,139 bp (1.7%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 142776 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 230 1302.2 0 230 7 313 1302.2 0 313 8 138 1302.2 0 138 9 26 1302.2 0 26 10 28 1302.2 0 28 11 47 1302.2 0 47 12 69 1302.2 0 69 13 28 1302.2 0 28 14 365 1302.2 0 365 15 29 1302.2 0 29 16 25 1302.2 0 25 17 24 1302.2 0 24 18 32 1302.2 0 32 19 33 1302.2 0 33 20 32 1302.2 0 32 21 33 1302.2 0 33 22 28 1302.2 0 28 23 35 1302.2 0 35 24 26 1302.2 0 26 25 28 1302.2 0 28 26 26 1302.2 0 26 27 26 1302.2 0 26 28 24 1302.2 0 24 29 107 1302.2 0 107 30 38 1302.2 0 38 31 17 1302.2 0 17 32 22 1302.2 0 22 33 31 1302.2 0 31 34 26 1302.2 0 26 35 16 1302.2 0 16 36 48 1302.2 0 48 37 39 1302.2 0 39 38 56 1302.2 0 56 39 175 1302.2 0 175 40 92 1302.2 0 92 41 222 1302.2 0 222 42 30 1302.2 0 30 43 42 1302.2 0 42 44 50 1302.2 0 50 45 35 1302.2 0 35 46 44 1302.2 0 44 47 37 1302.2 0 37 48 57 1302.2 0 57 49 135 1302.2 0 135 50 196 1302.2 0 196 51 346 1302.2 0 346 52 680 1302.2 0 680 53 694 1302.2 0 694 54 1182 1302.2 0 1182 55 2841 1302.2 0 2841 56 54208 1302.2 0 54208 57 3199 1302.2 0 3199 58 21138 1302.2 0 21138 59 97 1302.2 0 97 60 25 1302.2 0 25 61 27 1302.2 0 27 62 252 1302.2 0 252 63 24 1302.2 0 24 64 40 1302.2 0 40 65 18 1302.2 0 18 66 20 1302.2 0 20 67 21 1302.2 0 21 68 16 1302.2 0 16 69 23 1302.2 0 23 70 16 1302.2 0 16 71 21 1302.2 0 21 72 236 1302.2 0 236 73 27 1302.2 0 27 74 37 1302.2 0 37 75 55 1302.2 0 55 76 23 1302.2 0 23 77 34 1302.2 0 34 78 94 1302.2 0 94 79 124 1302.2 0 124 80 100 1302.2 0 100 81 114 1302.2 0 114 82 90 1302.2 0 90 83 626 1302.2 0 626 84 280 1302.2 0 280 85 492 1302.2 0 492 86 195 1302.2 0 195 87 503 1302.2 0 503 88 515 1302.2 0 515 89 327 1302.2 0 327 90 413 1302.2 0 413 91 108 1302.2 0 108 92 30 1302.2 0 30 93 370 1302.2 0 370 94 256 1302.2 0 256 95 92 1302.2 0 92 96 155 1302.2 0 155 97 35 1302.2 0 35 98 33 1302.2 0 33 99 35 1302.2 0 35 100 56 1302.2 0 56 101 93 1302.2 0 93 102 33 1302.2 0 33 103 116 1302.2 0 116 104 109 1302.2 0 109 105 110 1302.2 0 110 106 32 1302.2 0 32 107 40 1302.2 0 40 108 25 1302.2 0 25 109 18 1302.2 0 18 110 35 1302.2 0 35 111 78 1302.2 0 78 112 38 1302.2 0 38 113 61 1302.2 0 61 114 70 1302.2 0 70 115 42 1302.2 0 42 116 74 1302.2 0 74 117 86 1302.2 0 86 118 87 1302.2 0 87 119 873 1302.2 0 873 120 236 1302.2 0 236 121 247 1302.2 0 247 122 195 1302.2 0 195 123 181 1302.2 0 181 124 783 1302.2 0 783 125 1105 1302.2 0 1105 126 3508 1302.2 0 3508 127 191 1302.2 0 191 128 218 1302.2 0 218 129 52 1302.2 0 52 130 51 1302.2 0 51 131 113 1302.2 0 113 132 147 1302.2 0 147 133 118 1302.2 0 118 134 150 1302.2 0 150 135 103 1302.2 0 103 136 84 1302.2 0 84 137 108 1302.2 0 108 138 141 1302.2 0 141 139 191 1302.2 0 191 140 109 1302.2 0 109 141 127 1302.2 0 127 142 84 1302.2 0 84 143 82 1302.2 0 82 144 103 1302.2 0 103 145 236 1302.2 0 236 146 211 1302.2 0 211 147 708 1302.2 0 708 148 530 1302.2 0 530 149 1094 1302.2 0 1094 150 1527 1302.2 0 1527 151 336 1302.2 0 336 152 332 1302.2 0 332 153 173 1302.2 0 173 154 162 1302.2 0 162 155 107 1302.2 0 107 156 100 1302.2 0 100 157 155 1302.2 0 155 158 254 1302.2 0 254 159 248 1302.2 0 248 160 463 1302.2 0 463 161 154 1302.2 0 154 162 227 1302.2 0 227 163 144 1302.2 0 144 164 189 1302.2 0 189 165 263 1302.2 0 263 166 350 1302.2 0 350 167 907 1302.2 0 907 168 745 1302.2 0 745 169 873 1302.2 0 873 170 392 1302.2 0 392 171 481 1302.2 0 481 172 811 1302.2 0 811 173 434 1302.2 0 434 174 321 1302.2 0 321 175 287 1302.2 0 287 176 262 1302.2 0 262 177 399 1302.2 0 399 178 368 1302.2 0 368 179 217 1302.2 0 217 180 247 1302.2 0 247 181 328 1302.2 0 328 182 191 1302.2 0 191 183 173 1302.2 0 173 184 213 1302.2 0 213 185 159 1302.2 0 159 186 183 1302.2 0 183 187 81 1302.2 0 81 188 121 1302.2 0 121 189 143 1302.2 0 143 190 219 1302.2 0 219 191 333 1302.2 0 333 192 463 1302.2 0 463 193 340 1302.2 0 340 194 252 1302.2 0 252 195 360 1302.2 0 360 196 872 1302.2 0 872 197 1248 1302.2 0 1248 198 283 1302.2 0 283 199 387 1302.2 0 387 200 1977 1302.2 0 1977 201 893 1302.2 0 893 202 1302 1302.2 0 1302 203 109 1302.2 0 109 204 164 1302.2 0 164 205 138 1302.2 0 138 206 90 1302.2 0 90 207 174 1302.2 0 174 208 79 1302.2 0 79 209 83 1302.2 0 83 210 82 1302.2 0 82 211 63 1302.2 0 63 212 86 1302.2 0 86 213 151 1302.2 0 151 214 113 1302.2 0 113 215 134 1302.2 0 134 216 163 1302.2 0 163 217 190 1302.2 0 190 218 178 1302.2 0 178 219 225 1302.2 0 225 220 139 1302.2 0 139 221 230 1302.2 0 230 222 120 1302.2 0 120 223 194 1302.2 0 194 224 379 1302.2 0 379 225 245 1302.2 0 245 226 174 1302.2 0 174 227 155 1302.2 0 155 228 168 1302.2 0 168 229 318 1302.2 0 318 230 112 1302.2 0 112 231 154 1302.2 0 154 232 399 1302.2 0 399 233 226 1302.2 0 226 234 198 1302.2 0 198 235 82 1302.2 0 82 236 48 1302.2 0 48 237 41 1302.2 0 41 238 38 1302.2 0 38 239 40 1302.2 0 40 240 47 1302.2 0 47 241 95 1302.2 0 95 242 126 1302.2 0 126 243 124 1302.2 0 124 244 222 1302.2 0 222 245 146 1302.2 0 146 246 80 1302.2 0 80 247 58 1302.2 0 58 248 44 1302.2 0 44 249 46 1302.2 0 46 250 80 1302.2 0 80 251 228 1302.2 0 228 252 342 1302.2 0 342 253 408 1302.2 0 408 254 385 1302.2 0 385 255 289 1302.2 0 289 256 119 1302.2 0 119 257 62 1302.2 0 62 258 45 1302.2 0 45 259 32 1302.2 0 32 260 35 1302.2 0 35 261 20 1302.2 0 20 262 50 1302.2 0 50 263 138 1302.2 0 138 264 172 1302.2 0 172 265 284 1302.2 0 284 266 287 1302.2 0 287 267 49 1302.2 0 49 268 37 1302.2 0 37 269 51 1302.2 0 51 270 44 1302.2 0 44 271 121 1302.2 0 121 272 96 1302.2 0 96 273 143 1302.2 0 143 274 90 1302.2 0 90 275 81 1302.2 0 81 276 92 1302.2 0 92 277 120 1302.2 0 120 278 135 1302.2 0 135 279 267 1302.2 0 267 280 202 1302.2 0 202 281 72 1302.2 0 72 282 108 1302.2 0 108 283 265 1302.2 0 265 284 124 1302.2 0 124 285 58 1302.2 0 58 286 79 1302.2 0 79 287 71 1302.2 0 71 288 52 1302.2 0 52 289 54 1302.2 0 54 290 74 1302.2 0 74 291 156 1302.2 0 156 292 359 1302.2 0 359 293 285 1302.2 0 285 294 142 1302.2 0 142 295 70 1302.2 0 70 296 44 1302.2 0 44 297 36 1302.2 0 36 298 139 1302.2 0 139 299 151 1302.2 0 151 300 50 1302.2 0 50 301 35 1302.2 0 35 Finished in 5.53 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 127,876 Reads with adapters: 376 (0.3%) Reads that were too short: 114 (0.1%) Reads written (passing filters): 262 (0.2%) Total basepairs processed: 27,664,139 bp Total written (filtered): 58,331 bp (0.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 376 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 5 0.0 3 0 0 1 4 17 146 0.0 3 141 4 1 18 12 0.0 3 0 0 11 1 19 8 0.0 3 0 0 0 8 20 5 0.0 3 1 0 0 4 22 1 0.0 3 0 0 0 1 23 2 0.0 3 0 0 0 2 25 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 53 64 0.0 3 0 0 1 63 56 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 59 3 0.0 3 0 0 0 3 62 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 89 3 0.0 3 0 0 0 3 108 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 142 6 0.0 3 0 0 0 6 144 1 0.0 3 0 0 0 1 145 3 0.0 3 0 0 0 3 153 2 0.0 3 0 0 0 2 165 27 0.0 3 0 0 0 27 166 3 0.0 3 0 0 0 3 167 2 0.0 3 0 0 0 2 168 9 0.0 3 0 0 1 8 210 2 0.0 3 0 0 0 2 221 6 0.0 3 0 0 0 6 222 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 231 2 0.0 3 0 0 0 2 232 18 0.0 3 0 0 0 18 233 19 0.0 3 0 0 0 19 236 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 262 Reads with adapters: 12 (4.6%) Reads written (passing filters): 262 (100.0%) Total basepairs processed: 58,331 bp Total written (filtered): 58,291 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 12 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 41.7% C: 41.7% G: 8.3% T: 8.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 11 4.1 0 11 7 1 0.0 0 1