This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 175.29 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 216,125 (4.1%) Reads that were too short: 69,211 (1.3%) Reads written (passing filters): 146,914 (2.8%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 25,707,196 bp (1.6%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 216125 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 39 1302.2 0 39 7 3334 1302.2 0 3334 8 300 1302.2 0 300 9 8 1302.2 0 8 10 9 1302.2 0 9 11 7 1302.2 0 7 12 14 1302.2 0 14 13 11 1302.2 0 11 14 4153 1302.2 0 4153 15 558 1302.2 0 558 16 8 1302.2 0 8 17 8 1302.2 0 8 18 8 1302.2 0 8 19 11 1302.2 0 11 20 9 1302.2 0 9 21 5 1302.2 0 5 22 13 1302.2 0 13 23 9 1302.2 0 9 24 14 1302.2 0 14 25 15 1302.2 0 15 26 77 1302.2 0 77 27 62 1302.2 0 62 28 4 1302.2 0 4 29 6 1302.2 0 6 30 10 1302.2 0 10 31 5 1302.2 0 5 32 16 1302.2 0 16 33 15 1302.2 0 15 34 9 1302.2 0 9 35 15 1302.2 0 15 36 13 1302.2 0 13 37 7 1302.2 0 7 38 15 1302.2 0 15 39 9 1302.2 0 9 40 41 1302.2 0 41 41 10 1302.2 0 10 42 26 1302.2 0 26 43 22 1302.2 0 22 44 38 1302.2 0 38 45 13 1302.2 0 13 46 8 1302.2 0 8 47 11 1302.2 0 11 48 10 1302.2 0 10 49 13 1302.2 0 13 50 4 1302.2 0 4 51 11 1302.2 0 11 52 9 1302.2 0 9 53 10 1302.2 0 10 54 14 1302.2 0 14 55 19 1302.2 0 19 56 14 1302.2 0 14 57 18 1302.2 0 18 58 10 1302.2 0 10 59 8 1302.2 0 8 60 30 1302.2 0 30 61 8 1302.2 0 8 62 13 1302.2 0 13 63 14 1302.2 0 14 64 10 1302.2 0 10 65 46 1302.2 0 46 66 23 1302.2 0 23 67 22 1302.2 0 22 68 11 1302.2 0 11 69 15 1302.2 0 15 70 15 1302.2 0 15 71 14 1302.2 0 14 72 15 1302.2 0 15 73 8 1302.2 0 8 74 14 1302.2 0 14 75 20 1302.2 0 20 76 21 1302.2 0 21 77 15 1302.2 0 15 78 39 1302.2 0 39 79 64 1302.2 0 64 80 179 1302.2 0 179 81 398 1302.2 0 398 82 98 1302.2 0 98 83 185 1302.2 0 185 84 135 1302.2 0 135 85 70 1302.2 0 70 86 105 1302.2 0 105 87 454 1302.2 0 454 88 345 1302.2 0 345 89 82 1302.2 0 82 90 293 1302.2 0 293 91 686 1302.2 0 686 92 208 1302.2 0 208 93 210 1302.2 0 210 94 555 1302.2 0 555 95 321 1302.2 0 321 96 164 1302.2 0 164 97 419 1302.2 0 419 98 408 1302.2 0 408 99 350 1302.2 0 350 100 276 1302.2 0 276 101 720 1302.2 0 720 102 576 1302.2 0 576 103 877 1302.2 0 877 104 13890 1302.2 0 13890 105 19162 1302.2 0 19162 106 23883 1302.2 0 23883 107 313 1302.2 0 313 108 97 1302.2 0 97 109 33 1302.2 0 33 110 242 1302.2 0 242 111 235 1302.2 0 235 112 267 1302.2 0 267 113 238 1302.2 0 238 114 167 1302.2 0 167 115 96 1302.2 0 96 116 105 1302.2 0 105 117 190 1302.2 0 190 118 118 1302.2 0 118 119 1043 1302.2 0 1043 120 730 1302.2 0 730 121 417 1302.2 0 417 122 320 1302.2 0 320 123 323 1302.2 0 323 124 299 1302.2 0 299 125 365 1302.2 0 365 126 308 1302.2 0 308 127 252 1302.2 0 252 128 285 1302.2 0 285 129 371 1302.2 0 371 130 126 1302.2 0 126 131 261 1302.2 0 261 132 413 1302.2 0 413 133 308 1302.2 0 308 134 270 1302.2 0 270 135 234 1302.2 0 234 136 245 1302.2 0 245 137 338 1302.2 0 338 138 964 1302.2 0 964 139 719 1302.2 0 719 140 443 1302.2 0 443 141 268 1302.2 0 268 142 376 1302.2 0 376 143 308 1302.2 0 308 144 300 1302.2 0 300 145 613 1302.2 0 613 146 408 1302.2 0 408 147 229 1302.2 0 229 148 258 1302.2 0 258 149 114 1302.2 0 114 150 137 1302.2 0 137 151 275 1302.2 0 275 152 246 1302.2 0 246 153 358 1302.2 0 358 154 386 1302.2 0 386 155 588 1302.2 0 588 156 451 1302.2 0 451 157 731 1302.2 0 731 158 1449 1302.2 0 1449 159 12512 1302.2 0 12512 160 3463 1302.2 0 3463 161 17732 1302.2 0 17732 162 453 1302.2 0 453 163 490 1302.2 0 490 164 381 1302.2 0 381 165 417 1302.2 0 417 166 289 1302.2 0 289 167 745 1302.2 0 745 168 490 1302.2 0 490 169 409 1302.2 0 409 170 260 1302.2 0 260 171 785 1302.2 0 785 172 561 1302.2 0 561 173 405 1302.2 0 405 174 388 1302.2 0 388 175 154 1302.2 0 154 176 140 1302.2 0 140 177 228 1302.2 0 228 178 298 1302.2 0 298 179 405 1302.2 0 405 180 631 1302.2 0 631 181 654 1302.2 0 654 182 490 1302.2 0 490 183 428 1302.2 0 428 184 841 1302.2 0 841 185 627 1302.2 0 627 186 375 1302.2 0 375 187 251 1302.2 0 251 188 772 1302.2 0 772 189 367 1302.2 0 367 190 413 1302.2 0 413 191 301 1302.2 0 301 192 357 1302.2 0 357 193 164 1302.2 0 164 194 192 1302.2 0 192 195 352 1302.2 0 352 196 347 1302.2 0 347 197 180 1302.2 0 180 198 322 1302.2 0 322 199 453 1302.2 0 453 200 2724 1302.2 0 2724 201 1467 1302.2 0 1467 202 666 1302.2 0 666 203 298 1302.2 0 298 204 488 1302.2 0 488 205 559 1302.2 0 559 206 471 1302.2 0 471 207 720 1302.2 0 720 208 473 1302.2 0 473 209 304 1302.2 0 304 210 438 1302.2 0 438 211 427 1302.2 0 427 212 462 1302.2 0 462 213 414 1302.2 0 414 214 435 1302.2 0 435 215 691 1302.2 0 691 216 611 1302.2 0 611 217 733 1302.2 0 733 218 514 1302.2 0 514 219 458 1302.2 0 458 220 757 1302.2 0 757 221 1057 1302.2 0 1057 222 366 1302.2 0 366 223 363 1302.2 0 363 224 1017 1302.2 0 1017 225 684 1302.2 0 684 226 714 1302.2 0 714 227 604 1302.2 0 604 228 878 1302.2 0 878 229 537 1302.2 0 537 230 445 1302.2 0 445 231 311 1302.2 0 311 232 499 1302.2 0 499 233 324 1302.2 0 324 234 231 1302.2 0 231 235 539 1302.2 0 539 236 1036 1302.2 0 1036 237 353 1302.2 0 353 238 735 1302.2 0 735 239 755 1302.2 0 755 240 4985 1302.2 0 4985 241 2992 1302.2 0 2992 242 2272 1302.2 0 2272 243 754 1302.2 0 754 244 728 1302.2 0 728 245 625 1302.2 0 625 246 434 1302.2 0 434 247 419 1302.2 0 419 248 664 1302.2 0 664 249 624 1302.2 0 624 250 561 1302.2 0 561 251 431 1302.2 0 431 252 498 1302.2 0 498 253 1050 1302.2 0 1050 254 1253 1302.2 0 1253 255 918 1302.2 0 918 256 587 1302.2 0 587 257 402 1302.2 0 402 258 442 1302.2 0 442 259 297 1302.2 0 297 260 308 1302.2 0 308 261 355 1302.2 0 355 262 353 1302.2 0 353 263 664 1302.2 0 664 264 909 1302.2 0 909 265 852 1302.2 0 852 266 510 1302.2 0 510 267 404 1302.2 0 404 268 494 1302.2 0 494 269 670 1302.2 0 670 270 447 1302.2 0 447 271 569 1302.2 0 569 272 505 1302.2 0 505 273 713 1302.2 0 713 274 738 1302.2 0 738 275 841 1302.2 0 841 276 756 1302.2 0 756 277 597 1302.2 0 597 278 713 1302.2 0 713 279 780 1302.2 0 780 280 988 1302.2 0 988 281 1080 1302.2 0 1080 282 894 1302.2 0 894 283 847 1302.2 0 847 284 899 1302.2 0 899 285 543 1302.2 0 543 286 485 1302.2 0 485 287 355 1302.2 0 355 288 397 1302.2 0 397 289 391 1302.2 0 391 290 401 1302.2 0 401 291 481 1302.2 0 481 292 386 1302.2 0 386 293 413 1302.2 0 413 294 485 1302.2 0 485 295 260 1302.2 0 260 296 170 1302.2 0 170 297 412 1302.2 0 412 298 560 1302.2 0 560 299 839 1302.2 0 839 300 1951 1302.2 0 1951 301 1493 1302.2 0 1493 Finished in 6.82 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 146,914 Reads with adapters: 2,050 (1.4%) Reads that were too short: 26 (0.0%) Reads written (passing filters): 2,024 (1.4%) Total basepairs processed: 25,707,196 bp Total written (filtered): 355,642 bp (1.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2050 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 1 0.0 3 0 0 0 0 1 18 2 0.0 3 0 0 0 0 2 19 8 0.0 3 0 2 0 1 5 20 159 0.0 4 152 2 0 3 2 21 4 0.0 4 0 0 0 3 1 22 5 0.0 4 0 0 0 0 5 24 2 0.0 4 0 0 0 0 2 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 30 1828 0.0 4 0 0 0 0 1828 31 1 0.0 4 0 0 0 0 1 32 2 0.0 4 0 0 0 0 2 33 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 48 2 0.0 4 0 0 0 0 2 49 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 0 2 115 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 127 6 0.0 4 0 0 0 0 6 128 1 0.0 4 0 0 0 0 1 137 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 185 3 0.0 4 0 0 0 0 3 186 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (30 us/read; 2.02 M reads/minute). === Summary === Total reads processed: 2,024 Reads with adapters: 22 (1.1%) Reads written (passing filters): 2,024 (100.0%) Total basepairs processed: 355,642 bp Total written (filtered): 354,218 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 22 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 72.7% C: 0.0% G: 27.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 31.6 0 4 4 7 7.9 0 7 11 6 0.0 1 0 6 258 1 0.0 1 1 259 1 0.0 1 0 1 267 3 0.0 1 3