This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 171.46 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 291,314 (5.5%) Reads that were too short: 73,463 (1.4%) Reads written (passing filters): 217,851 (4.1%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 51,176,015 bp (3.2%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 291314 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 634 1302.2 0 634 7 679 1302.2 0 679 8 1045 1302.2 0 1045 9 78356 1302.2 0 78356 10 112 1302.2 0 112 11 18 1302.2 0 18 12 26 1302.2 0 26 13 17 1302.2 0 17 14 19 1302.2 0 19 15 17 1302.2 0 17 16 21 1302.2 0 21 17 29 1302.2 0 29 18 22 1302.2 0 22 19 17 1302.2 0 17 20 24 1302.2 0 24 21 17 1302.2 0 17 22 32 1302.2 0 32 23 19 1302.2 0 19 24 29 1302.2 0 29 25 41 1302.2 0 41 26 77 1302.2 0 77 27 22 1302.2 0 22 28 44 1302.2 0 44 29 89 1302.2 0 89 30 47 1302.2 0 47 31 1123 1302.2 0 1123 32 85 1302.2 0 85 33 52 1302.2 0 52 34 50 1302.2 0 50 35 143 1302.2 0 143 36 68 1302.2 0 68 37 56 1302.2 0 56 38 125 1302.2 0 125 39 70 1302.2 0 70 40 103 1302.2 0 103 41 136 1302.2 0 136 42 2045 1302.2 0 2045 43 2824 1302.2 0 2824 44 3354 1302.2 0 3354 45 36 1302.2 0 36 46 28 1302.2 0 28 47 54 1302.2 0 54 48 168 1302.2 0 168 49 45 1302.2 0 45 50 77 1302.2 0 77 51 34 1302.2 0 34 52 33 1302.2 0 33 53 60 1302.2 0 60 54 41 1302.2 0 41 55 82 1302.2 0 82 56 79 1302.2 0 79 57 77 1302.2 0 77 58 96 1302.2 0 96 59 150 1302.2 0 150 60 281 1302.2 0 281 61 445 1302.2 0 445 62 511 1302.2 0 511 63 1046 1302.2 0 1046 64 1948 1302.2 0 1948 65 30205 1302.2 0 30205 66 9297 1302.2 0 9297 67 18788 1302.2 0 18788 68 108 1302.2 0 108 69 24 1302.2 0 24 70 18 1302.2 0 18 71 14 1302.2 0 14 72 7 1302.2 0 7 73 23 1302.2 0 23 74 52 1302.2 0 52 75 176 1302.2 0 176 76 120 1302.2 0 120 77 83 1302.2 0 83 78 174 1302.2 0 174 79 131 1302.2 0 131 80 241 1302.2 0 241 81 588 1302.2 0 588 82 243 1302.2 0 243 83 78 1302.2 0 78 84 288 1302.2 0 288 85 212 1302.2 0 212 86 372 1302.2 0 372 87 1420 1302.2 0 1420 88 450 1302.2 0 450 89 184 1302.2 0 184 90 553 1302.2 0 553 91 410 1302.2 0 410 92 216 1302.2 0 216 93 169 1302.2 0 169 94 411 1302.2 0 411 95 293 1302.2 0 293 96 1187 1302.2 0 1187 97 1315 1302.2 0 1315 98 275 1302.2 0 275 99 113 1302.2 0 113 100 250 1302.2 0 250 101 373 1302.2 0 373 102 724 1302.2 0 724 103 371 1302.2 0 371 104 310 1302.2 0 310 105 51 1302.2 0 51 106 115 1302.2 0 115 107 267 1302.2 0 267 108 318 1302.2 0 318 109 45 1302.2 0 45 110 59 1302.2 0 59 111 136 1302.2 0 136 112 486 1302.2 0 486 113 249 1302.2 0 249 114 228 1302.2 0 228 115 326 1302.2 0 326 116 237 1302.2 0 237 117 227 1302.2 0 227 118 208 1302.2 0 208 119 551 1302.2 0 551 120 595 1302.2 0 595 121 431 1302.2 0 431 122 483 1302.2 0 483 123 341 1302.2 0 341 124 279 1302.2 0 279 125 349 1302.2 0 349 126 346 1302.2 0 346 127 465 1302.2 0 465 128 691 1302.2 0 691 129 409 1302.2 0 409 130 210 1302.2 0 210 131 213 1302.2 0 213 132 493 1302.2 0 493 133 1350 1302.2 0 1350 134 1205 1302.2 0 1205 135 582 1302.2 0 582 136 665 1302.2 0 665 137 957 1302.2 0 957 138 1660 1302.2 0 1660 139 977 1302.2 0 977 140 684 1302.2 0 684 141 707 1302.2 0 707 142 492 1302.2 0 492 143 401 1302.2 0 401 144 352 1302.2 0 352 145 300 1302.2 0 300 146 423 1302.2 0 423 147 436 1302.2 0 436 148 474 1302.2 0 474 149 687 1302.2 0 687 150 649 1302.2 0 649 151 352 1302.2 0 352 152 186 1302.2 0 186 153 200 1302.2 0 200 154 378 1302.2 0 378 155 580 1302.2 0 580 156 383 1302.2 0 383 157 350 1302.2 0 350 158 573 1302.2 0 573 159 1309 1302.2 0 1309 160 826 1302.2 0 826 161 640 1302.2 0 640 162 391 1302.2 0 391 163 577 1302.2 0 577 164 597 1302.2 0 597 165 702 1302.2 0 702 166 564 1302.2 0 564 167 474 1302.2 0 474 168 567 1302.2 0 567 169 344 1302.2 0 344 170 691 1302.2 0 691 171 855 1302.2 0 855 172 596 1302.2 0 596 173 333 1302.2 0 333 174 437 1302.2 0 437 175 584 1302.2 0 584 176 612 1302.2 0 612 177 376 1302.2 0 376 178 230 1302.2 0 230 179 263 1302.2 0 263 180 360 1302.2 0 360 181 743 1302.2 0 743 182 803 1302.2 0 803 183 650 1302.2 0 650 184 687 1302.2 0 687 185 775 1302.2 0 775 186 1013 1302.2 0 1013 187 932 1302.2 0 932 188 761 1302.2 0 761 189 515 1302.2 0 515 190 276 1302.2 0 276 191 267 1302.2 0 267 192 367 1302.2 0 367 193 371 1302.2 0 371 194 688 1302.2 0 688 195 823 1302.2 0 823 196 504 1302.2 0 504 197 212 1302.2 0 212 198 199 1302.2 0 199 199 516 1302.2 0 516 200 1572 1302.2 0 1572 201 794 1302.2 0 794 202 291 1302.2 0 291 203 222 1302.2 0 222 204 361 1302.2 0 361 205 442 1302.2 0 442 206 1086 1302.2 0 1086 207 1426 1302.2 0 1426 208 994 1302.2 0 994 209 476 1302.2 0 476 210 477 1302.2 0 477 211 662 1302.2 0 662 212 568 1302.2 0 568 213 460 1302.2 0 460 214 625 1302.2 0 625 215 733 1302.2 0 733 216 913 1302.2 0 913 217 1013 1302.2 0 1013 218 675 1302.2 0 675 219 1246 1302.2 0 1246 220 1452 1302.2 0 1452 221 787 1302.2 0 787 222 341 1302.2 0 341 223 289 1302.2 0 289 224 1085 1302.2 0 1085 225 833 1302.2 0 833 226 755 1302.2 0 755 227 713 1302.2 0 713 228 987 1302.2 0 987 229 860 1302.2 0 860 230 962 1302.2 0 962 231 1118 1302.2 0 1118 232 491 1302.2 0 491 233 249 1302.2 0 249 234 166 1302.2 0 166 235 374 1302.2 0 374 236 947 1302.2 0 947 237 794 1302.2 0 794 238 618 1302.2 0 618 239 475 1302.2 0 475 240 550 1302.2 0 550 241 418 1302.2 0 418 242 278 1302.2 0 278 243 417 1302.2 0 417 244 774 1302.2 0 774 245 990 1302.2 0 990 246 1160 1302.2 0 1160 247 830 1302.2 0 830 248 900 1302.2 0 900 249 988 1302.2 0 988 250 545 1302.2 0 545 251 223 1302.2 0 223 252 129 1302.2 0 129 253 324 1302.2 0 324 254 856 1302.2 0 856 255 2183 1302.2 0 2183 256 2214 1302.2 0 2214 257 1956 1302.2 0 1956 258 1770 1302.2 0 1770 259 950 1302.2 0 950 260 594 1302.2 0 594 261 432 1302.2 0 432 262 436 1302.2 0 436 263 228 1302.2 0 228 264 301 1302.2 0 301 265 281 1302.2 0 281 266 577 1302.2 0 577 267 1180 1302.2 0 1180 268 1846 1302.2 0 1846 269 1220 1302.2 0 1220 270 622 1302.2 0 622 271 511 1302.2 0 511 272 379 1302.2 0 379 273 345 1302.2 0 345 274 661 1302.2 0 661 275 918 1302.2 0 918 276 572 1302.2 0 572 277 277 1302.2 0 277 278 253 1302.2 0 253 279 513 1302.2 0 513 280 925 1302.2 0 925 281 1083 1302.2 0 1083 282 659 1302.2 0 659 283 522 1302.2 0 522 284 322 1302.2 0 322 285 564 1302.2 0 564 286 641 1302.2 0 641 287 704 1302.2 0 704 288 564 1302.2 0 564 289 658 1302.2 0 658 290 552 1302.2 0 552 291 456 1302.2 0 456 292 398 1302.2 0 398 293 674 1302.2 0 674 294 1319 1302.2 0 1319 295 1138 1302.2 0 1138 296 628 1302.2 0 628 297 726 1302.2 0 726 298 631 1302.2 0 631 299 494 1302.2 0 494 300 470 1302.2 0 470 301 1768 1302.2 0 1768 Finished in 10.00 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 217,851 Reads with adapters: 80,097 (36.8%) Reads that were too short: 35 (0.0%) Reads written (passing filters): 80,062 (36.8%) Total basepairs processed: 51,176,015 bp Total written (filtered): 22,014,549 bp (43.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 80097 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 27 0.0 3 0 0 21 6 16 622 0.0 3 0 573 21 28 17 78974 0.0 3 76388 2277 238 71 18 197 0.0 3 12 153 23 9 19 56 0.0 3 0 0 20 36 20 142 0.0 3 1 0 0 141 22 1 0.0 3 1 27 1 0.0 3 0 0 0 1 32 1 0.0 3 0 1 33 2 0.0 3 1 0 0 1 34 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 38 1 0.0 3 1 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 61 5 0.0 3 0 0 0 5 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 111 2 0.0 3 0 0 0 2 116 1 0.0 3 1 125 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 0 3 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 223 2 0.0 3 0 0 0 2 224 5 0.0 3 0 0 0 5 225 3 0.0 3 0 0 0 3 230 5 0.0 3 0 0 0 5 231 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.65 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 80,062 Reads with adapters: 2,864 (3.6%) Reads written (passing filters): 80,062 (100.0%) Total basepairs processed: 22,014,549 bp Total written (filtered): 22,005,657 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2864 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 51.9% C: 7.6% G: 30.0% T: 10.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2574 1251.0 0 2574 4 287 312.7 0 287 6 2 19.5 0 2 10 1 0.1 1 0 1