This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 161.00 s (30 us/read; 1.99 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 109,454 (2.1%) Reads that were too short: 8,579 (0.2%) Reads written (passing filters): 100,875 (1.9%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 27,908,857 bp (1.7%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 109454 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2111 1302.2 0 2111 7 78192 1302.2 0 78192 8 240 1302.2 0 240 9 6816 1302.2 0 6816 10 15 1302.2 0 15 11 8 1302.2 0 8 12 6 1302.2 0 6 13 7 1302.2 0 7 14 10 1302.2 0 10 15 7 1302.2 0 7 16 12 1302.2 0 12 17 7 1302.2 0 7 18 4 1302.2 0 4 19 8 1302.2 0 8 21 6 1302.2 0 6 22 7 1302.2 0 7 23 9 1302.2 0 9 24 20 1302.2 0 20 25 14 1302.2 0 14 26 28 1302.2 0 28 27 19 1302.2 0 19 28 75 1302.2 0 75 29 28 1302.2 0 28 30 52 1302.2 0 52 31 38 1302.2 0 38 32 4 1302.2 0 4 33 18 1302.2 0 18 34 7 1302.2 0 7 35 5 1302.2 0 5 36 3 1302.2 0 3 37 7 1302.2 0 7 38 258 1302.2 0 258 39 15 1302.2 0 15 40 8 1302.2 0 8 41 6 1302.2 0 6 42 7 1302.2 0 7 43 8 1302.2 0 8 44 10 1302.2 0 10 45 9 1302.2 0 9 46 11 1302.2 0 11 47 9 1302.2 0 9 48 6 1302.2 0 6 49 17 1302.2 0 17 50 10 1302.2 0 10 51 15 1302.2 0 15 52 13 1302.2 0 13 53 37 1302.2 0 37 54 10 1302.2 0 10 55 3 1302.2 0 3 56 12 1302.2 0 12 57 6 1302.2 0 6 58 15 1302.2 0 15 59 7 1302.2 0 7 60 4 1302.2 0 4 61 9 1302.2 0 9 62 7 1302.2 0 7 63 11 1302.2 0 11 64 8 1302.2 0 8 65 5 1302.2 0 5 66 26 1302.2 0 26 67 42 1302.2 0 42 68 40 1302.2 0 40 69 138 1302.2 0 138 70 17 1302.2 0 17 71 33 1302.2 0 33 72 12 1302.2 0 12 73 11 1302.2 0 11 74 7 1302.2 0 7 75 10 1302.2 0 10 76 16 1302.2 0 16 77 133 1302.2 0 133 78 21 1302.2 0 21 79 76 1302.2 0 76 80 8 1302.2 0 8 81 10 1302.2 0 10 82 18 1302.2 0 18 83 6 1302.2 0 6 84 12 1302.2 0 12 85 7 1302.2 0 7 86 7 1302.2 0 7 87 7 1302.2 0 7 88 10 1302.2 0 10 89 7 1302.2 0 7 90 5 1302.2 0 5 91 13 1302.2 0 13 92 14 1302.2 0 14 93 19 1302.2 0 19 94 16 1302.2 0 16 95 10 1302.2 0 10 96 16 1302.2 0 16 97 29 1302.2 0 29 98 257 1302.2 0 257 99 340 1302.2 0 340 100 19 1302.2 0 19 101 16 1302.2 0 16 102 13 1302.2 0 13 103 6 1302.2 0 6 104 14 1302.2 0 14 105 9 1302.2 0 9 106 9 1302.2 0 9 107 11 1302.2 0 11 108 12 1302.2 0 12 109 10 1302.2 0 10 110 9 1302.2 0 9 111 14 1302.2 0 14 112 23 1302.2 0 23 113 25 1302.2 0 25 114 125 1302.2 0 125 115 141 1302.2 0 141 116 180 1302.2 0 180 117 16 1302.2 0 16 118 9 1302.2 0 9 119 14 1302.2 0 14 120 27 1302.2 0 27 121 32 1302.2 0 32 122 17 1302.2 0 17 123 40 1302.2 0 40 124 26 1302.2 0 26 125 19 1302.2 0 19 126 23 1302.2 0 23 127 21 1302.2 0 21 128 27 1302.2 0 27 129 58 1302.2 0 58 130 72 1302.2 0 72 131 49 1302.2 0 49 132 48 1302.2 0 48 133 118 1302.2 0 118 134 102 1302.2 0 102 135 89 1302.2 0 89 136 79 1302.2 0 79 137 101 1302.2 0 101 138 139 1302.2 0 139 139 95 1302.2 0 95 140 87 1302.2 0 87 141 146 1302.2 0 146 142 106 1302.2 0 106 143 743 1302.2 0 743 144 934 1302.2 0 934 145 2820 1302.2 0 2820 146 49 1302.2 0 49 147 58 1302.2 0 58 148 68 1302.2 0 68 149 642 1302.2 0 642 150 684 1302.2 0 684 151 293 1302.2 0 293 152 85 1302.2 0 85 153 23 1302.2 0 23 154 80 1302.2 0 80 155 37 1302.2 0 37 156 39 1302.2 0 39 157 33 1302.2 0 33 158 25 1302.2 0 25 159 40 1302.2 0 40 160 47 1302.2 0 47 161 51 1302.2 0 51 162 43 1302.2 0 43 163 58 1302.2 0 58 164 51 1302.2 0 51 165 43 1302.2 0 43 166 62 1302.2 0 62 167 49 1302.2 0 49 168 78 1302.2 0 78 169 39 1302.2 0 39 170 27 1302.2 0 27 171 30 1302.2 0 30 172 54 1302.2 0 54 173 29 1302.2 0 29 174 21 1302.2 0 21 175 27 1302.2 0 27 176 21 1302.2 0 21 177 34 1302.2 0 34 178 34 1302.2 0 34 179 51 1302.2 0 51 180 29 1302.2 0 29 181 48 1302.2 0 48 182 83 1302.2 0 83 183 59 1302.2 0 59 184 122 1302.2 0 122 185 63 1302.2 0 63 186 58 1302.2 0 58 187 16 1302.2 0 16 188 36 1302.2 0 36 189 106 1302.2 0 106 190 116 1302.2 0 116 191 242 1302.2 0 242 192 107 1302.2 0 107 193 43 1302.2 0 43 194 36 1302.2 0 36 195 39 1302.2 0 39 196 114 1302.2 0 114 197 46 1302.2 0 46 198 19 1302.2 0 19 199 16 1302.2 0 16 200 10 1302.2 0 10 201 57 1302.2 0 57 202 54 1302.2 0 54 203 34 1302.2 0 34 204 54 1302.2 0 54 205 64 1302.2 0 64 206 72 1302.2 0 72 207 39 1302.2 0 39 208 47 1302.2 0 47 209 73 1302.2 0 73 210 107 1302.2 0 107 211 177 1302.2 0 177 212 83 1302.2 0 83 213 93 1302.2 0 93 214 81 1302.2 0 81 215 129 1302.2 0 129 216 121 1302.2 0 121 217 100 1302.2 0 100 218 51 1302.2 0 51 219 29 1302.2 0 29 220 38 1302.2 0 38 221 70 1302.2 0 70 222 105 1302.2 0 105 223 47 1302.2 0 47 224 59 1302.2 0 59 225 110 1302.2 0 110 226 93 1302.2 0 93 227 90 1302.2 0 90 228 101 1302.2 0 101 229 42 1302.2 0 42 230 50 1302.2 0 50 231 28 1302.2 0 28 232 40 1302.2 0 40 233 84 1302.2 0 84 234 71 1302.2 0 71 235 64 1302.2 0 64 236 91 1302.2 0 91 237 68 1302.2 0 68 238 56 1302.2 0 56 239 40 1302.2 0 40 240 37 1302.2 0 37 241 49 1302.2 0 49 242 40 1302.2 0 40 243 49 1302.2 0 49 244 51 1302.2 0 51 245 68 1302.2 0 68 246 65 1302.2 0 65 247 27 1302.2 0 27 248 30 1302.2 0 30 249 284 1302.2 0 284 250 188 1302.2 0 188 251 171 1302.2 0 171 252 33 1302.2 0 33 253 109 1302.2 0 109 254 266 1302.2 0 266 255 346 1302.2 0 346 256 73 1302.2 0 73 257 42 1302.2 0 42 258 52 1302.2 0 52 259 60 1302.2 0 60 260 34 1302.2 0 34 261 69 1302.2 0 69 262 65 1302.2 0 65 263 50 1302.2 0 50 264 80 1302.2 0 80 265 114 1302.2 0 114 266 79 1302.2 0 79 267 29 1302.2 0 29 268 36 1302.2 0 36 269 361 1302.2 0 361 270 680 1302.2 0 680 271 90 1302.2 0 90 272 48 1302.2 0 48 273 74 1302.2 0 74 274 86 1302.2 0 86 275 126 1302.2 0 126 276 61 1302.2 0 61 277 91 1302.2 0 91 278 73 1302.2 0 73 279 35 1302.2 0 35 280 45 1302.2 0 45 281 75 1302.2 0 75 282 142 1302.2 0 142 283 124 1302.2 0 124 284 56 1302.2 0 56 285 78 1302.2 0 78 286 61 1302.2 0 61 287 39 1302.2 0 39 288 30 1302.2 0 30 289 81 1302.2 0 81 290 75 1302.2 0 75 291 73 1302.2 0 73 292 46 1302.2 0 46 293 36 1302.2 0 36 294 69 1302.2 0 69 295 29 1302.2 0 29 296 39 1302.2 0 39 297 24 1302.2 0 24 298 76 1302.2 0 76 299 111 1302.2 0 111 300 120 1302.2 0 120 301 74 1302.2 0 74 Finished in 5.02 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 100,875 Reads with adapters: 80,216 (79.5%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 80,211 (79.5%) Total basepairs processed: 27,908,857 bp Total written (filtered): 21,975,182 bp (78.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 80216 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 30 0.0 3 0 0 29 1 19 1037 0.0 3 0 1005 26 3 3 20 78881 0.0 4 77685 1152 31 4 9 21 152 0.0 4 27 123 2 22 11 0.0 4 4 1 6 23 43 0.0 4 0 0 0 39 4 24 4 0.0 4 2 0 0 2 29 1 0.0 4 1 34 1 0.0 4 0 0 0 0 1 36 1 0.0 4 1 40 1 0.0 4 0 0 0 0 1 41 1 0.0 4 1 91 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 44 0.0 4 0 0 0 0 44 167 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.38 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 80,211 Reads with adapters: 265 (0.3%) Reads written (passing filters): 80,211 (100.0%) Total basepairs processed: 21,975,182 bp Total written (filtered): 21,968,995 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 265 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.0% C: 3.0% G: 88.7% T: 5.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 1253.3 0 5 4 6 313.3 0 6 5 1 78.3 0 1 10 2 0.1 1 0 2 11 221 0.0 1 2 219 12 11 0.0 1 0 11 15 1 0.0 1 0 1 85 1 0.0 1 1 127 1 0.0 1 0 1 140 1 0.0 1 1 152 1 0.0 1 1 183 5 0.0 1 5 210 1 0.0 1 0 1 225 5 0.0 1 5 259 2 0.0 1 2 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.