This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 168.29 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 128,103 (2.4%) Reads that were too short: 35,732 (0.7%) Reads written (passing filters): 92,371 (1.7%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 23,519,158 bp (1.5%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 128103 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 663 1302.2 0 663 7 829 1302.2 0 829 8 62070 1302.2 0 62070 9 128 1302.2 0 128 10 23 1302.2 0 23 11 21 1302.2 0 21 12 16 1302.2 0 16 13 19 1302.2 0 19 14 14 1302.2 0 14 15 19 1302.2 0 19 16 20 1302.2 0 20 17 28 1302.2 0 28 18 32 1302.2 0 32 19 12 1302.2 0 12 20 23 1302.2 0 23 21 22 1302.2 0 22 22 624 1302.2 0 624 23 28 1302.2 0 28 24 35 1302.2 0 35 25 27 1302.2 0 27 26 42 1302.2 0 42 27 171 1302.2 0 171 28 23 1302.2 0 23 29 21 1302.2 0 21 30 29 1302.2 0 29 31 159 1302.2 0 159 32 19 1302.2 0 19 33 20 1302.2 0 20 34 27 1302.2 0 27 35 72 1302.2 0 72 36 45 1302.2 0 45 37 143 1302.2 0 143 38 22 1302.2 0 22 39 20 1302.2 0 20 40 24 1302.2 0 24 41 44 1302.2 0 44 42 16 1302.2 0 16 43 17 1302.2 0 17 44 17 1302.2 0 17 45 29 1302.2 0 29 46 25 1302.2 0 25 47 21 1302.2 0 21 48 15 1302.2 0 15 49 15 1302.2 0 15 50 23 1302.2 0 23 51 9 1302.2 0 9 52 20 1302.2 0 20 53 18 1302.2 0 18 54 80 1302.2 0 80 55 19 1302.2 0 19 56 15 1302.2 0 15 57 36 1302.2 0 36 58 28 1302.2 0 28 59 18 1302.2 0 18 60 10 1302.2 0 10 61 15 1302.2 0 15 62 15 1302.2 0 15 63 29 1302.2 0 29 64 19 1302.2 0 19 65 12 1302.2 0 12 66 16 1302.2 0 16 67 21 1302.2 0 21 68 24 1302.2 0 24 69 74 1302.2 0 74 70 18 1302.2 0 18 71 62 1302.2 0 62 72 23 1302.2 0 23 73 39 1302.2 0 39 74 23 1302.2 0 23 75 65 1302.2 0 65 76 274 1302.2 0 274 77 120 1302.2 0 120 78 374 1302.2 0 374 79 106 1302.2 0 106 80 315 1302.2 0 315 81 263 1302.2 0 263 82 390 1302.2 0 390 83 129 1302.2 0 129 84 304 1302.2 0 304 85 101 1302.2 0 101 86 72 1302.2 0 72 87 103 1302.2 0 103 88 189 1302.2 0 189 89 53 1302.2 0 53 90 44 1302.2 0 44 91 139 1302.2 0 139 92 159 1302.2 0 159 93 85 1302.2 0 85 94 275 1302.2 0 275 95 119 1302.2 0 119 96 488 1302.2 0 488 97 826 1302.2 0 826 98 1642 1302.2 0 1642 99 164 1302.2 0 164 100 83 1302.2 0 83 101 39 1302.2 0 39 102 38 1302.2 0 38 103 27 1302.2 0 27 104 31 1302.2 0 31 105 26 1302.2 0 26 106 21 1302.2 0 21 107 58 1302.2 0 58 108 127 1302.2 0 127 109 112 1302.2 0 112 110 62 1302.2 0 62 111 60 1302.2 0 60 112 127 1302.2 0 127 113 124 1302.2 0 124 114 270 1302.2 0 270 115 205 1302.2 0 205 116 163 1302.2 0 163 117 91 1302.2 0 91 118 63 1302.2 0 63 119 74 1302.2 0 74 120 166 1302.2 0 166 121 92 1302.2 0 92 122 121 1302.2 0 121 123 76 1302.2 0 76 124 93 1302.2 0 93 125 76 1302.2 0 76 126 89 1302.2 0 89 127 106 1302.2 0 106 128 146 1302.2 0 146 129 294 1302.2 0 294 130 403 1302.2 0 403 131 320 1302.2 0 320 132 318 1302.2 0 318 133 354 1302.2 0 354 134 528 1302.2 0 528 135 187 1302.2 0 187 136 149 1302.2 0 149 137 138 1302.2 0 138 138 121 1302.2 0 121 139 193 1302.2 0 193 140 107 1302.2 0 107 141 172 1302.2 0 172 142 112 1302.2 0 112 143 174 1302.2 0 174 144 106 1302.2 0 106 145 109 1302.2 0 109 146 112 1302.2 0 112 147 114 1302.2 0 114 148 160 1302.2 0 160 149 176 1302.2 0 176 150 308 1302.2 0 308 151 107 1302.2 0 107 152 93 1302.2 0 93 153 109 1302.2 0 109 154 129 1302.2 0 129 155 197 1302.2 0 197 156 242 1302.2 0 242 157 165 1302.2 0 165 158 184 1302.2 0 184 159 242 1302.2 0 242 160 204 1302.2 0 204 161 199 1302.2 0 199 162 210 1302.2 0 210 163 203 1302.2 0 203 164 263 1302.2 0 263 165 223 1302.2 0 223 166 217 1302.2 0 217 167 232 1302.2 0 232 168 335 1302.2 0 335 169 153 1302.2 0 153 170 149 1302.2 0 149 171 126 1302.2 0 126 172 256 1302.2 0 256 173 90 1302.2 0 90 174 85 1302.2 0 85 175 91 1302.2 0 91 176 158 1302.2 0 158 177 151 1302.2 0 151 178 218 1302.2 0 218 179 145 1302.2 0 145 180 114 1302.2 0 114 181 180 1302.2 0 180 182 288 1302.2 0 288 183 397 1302.2 0 397 184 393 1302.2 0 393 185 352 1302.2 0 352 186 286 1302.2 0 286 187 194 1302.2 0 194 188 353 1302.2 0 353 189 1566 1302.2 0 1566 190 577 1302.2 0 577 191 299 1302.2 0 299 192 219 1302.2 0 219 193 151 1302.2 0 151 194 136 1302.2 0 136 195 138 1302.2 0 138 196 316 1302.2 0 316 197 178 1302.2 0 178 198 81 1302.2 0 81 199 63 1302.2 0 63 200 126 1302.2 0 126 201 213 1302.2 0 213 202 224 1302.2 0 224 203 136 1302.2 0 136 204 180 1302.2 0 180 205 235 1302.2 0 235 206 228 1302.2 0 228 207 282 1302.2 0 282 208 370 1302.2 0 370 209 595 1302.2 0 595 210 716 1302.2 0 716 211 1171 1302.2 0 1171 212 349 1302.2 0 349 213 344 1302.2 0 344 214 413 1302.2 0 413 215 355 1302.2 0 355 216 410 1302.2 0 410 217 320 1302.2 0 320 218 244 1302.2 0 244 219 140 1302.2 0 140 220 164 1302.2 0 164 221 299 1302.2 0 299 222 340 1302.2 0 340 223 200 1302.2 0 200 224 241 1302.2 0 241 225 433 1302.2 0 433 226 427 1302.2 0 427 227 312 1302.2 0 312 228 333 1302.2 0 333 229 243 1302.2 0 243 230 226 1302.2 0 226 231 187 1302.2 0 187 232 247 1302.2 0 247 233 396 1302.2 0 396 234 269 1302.2 0 269 235 246 1302.2 0 246 236 269 1302.2 0 269 237 257 1302.2 0 257 238 221 1302.2 0 221 239 211 1302.2 0 211 240 563 1302.2 0 563 241 1208 1302.2 0 1208 242 3023 1302.2 0 3023 243 488 1302.2 0 488 244 247 1302.2 0 247 245 309 1302.2 0 309 246 231 1302.2 0 231 247 205 1302.2 0 205 248 312 1302.2 0 312 249 656 1302.2 0 656 250 1193 1302.2 0 1193 251 1656 1302.2 0 1656 252 185 1302.2 0 185 253 274 1302.2 0 274 254 424 1302.2 0 424 255 552 1302.2 0 552 256 225 1302.2 0 225 257 163 1302.2 0 163 258 173 1302.2 0 173 259 154 1302.2 0 154 260 215 1302.2 0 215 261 302 1302.2 0 302 262 399 1302.2 0 399 263 204 1302.2 0 204 264 284 1302.2 0 284 265 244 1302.2 0 244 266 243 1302.2 0 243 267 229 1302.2 0 229 268 250 1302.2 0 250 269 637 1302.2 0 637 270 406 1302.2 0 406 271 807 1302.2 0 807 272 331 1302.2 0 331 273 219 1302.2 0 219 274 280 1302.2 0 280 275 211 1302.2 0 211 276 245 1302.2 0 245 277 243 1302.2 0 243 278 192 1302.2 0 192 279 145 1302.2 0 145 280 169 1302.2 0 169 281 206 1302.2 0 206 282 180 1302.2 0 180 283 194 1302.2 0 194 284 202 1302.2 0 202 285 206 1302.2 0 206 286 95 1302.2 0 95 287 176 1302.2 0 176 288 169 1302.2 0 169 289 343 1302.2 0 343 290 317 1302.2 0 317 291 288 1302.2 0 288 292 203 1302.2 0 203 293 186 1302.2 0 186 294 192 1302.2 0 192 295 199 1302.2 0 199 296 189 1302.2 0 189 297 307 1302.2 0 307 298 262 1302.2 0 262 299 389 1302.2 0 389 300 224 1302.2 0 224 301 476 1302.2 0 476 Finished in 4.28 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 92,371 Reads with adapters: 63,123 (68.3%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 63,109 (68.3%) Total basepairs processed: 23,519,158 bp Total written (filtered): 17,415,038 bp (74.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 63123 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 25 0.0 3 0 0 21 4 16 647 0.0 3 0 581 47 19 17 61492 0.0 3 59683 1595 188 26 18 160 0.0 3 6 117 29 8 19 126 0.0 3 0 2 95 29 20 632 0.0 3 242 8 1 381 30 1 0.0 3 0 0 0 1 33 1 0.0 3 1 37 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 64 2 0.0 3 0 0 0 2 70 4 0.0 3 0 0 0 4 88 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 162 2 0.0 3 0 0 0 2 163 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 186 3 0.0 3 0 0 1 2 187 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.84 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 63,109 Reads with adapters: 706 (1.1%) Reads written (passing filters): 63,109 (100.0%) Total basepairs processed: 17,415,038 bp Total written (filtered): 17,412,817 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 706 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.9% C: 10.9% G: 23.5% T: 22.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 617 986.1 0 617 4 78 246.5 0 78 5 10 61.6 0 10 8 1 1.0 0 1