This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 171.06 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 156,360 (2.9%) Reads that were too short: 38,628 (0.7%) Reads written (passing filters): 117,732 (2.2%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 30,905,861 bp (1.9%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 156360 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 809 1302.2 0 809 7 1065 1302.2 0 1065 8 29009 1302.2 0 29009 9 60819 1302.2 0 60819 10 96 1302.2 0 96 11 4 1302.2 0 4 12 2 1302.2 0 2 13 1 1302.2 0 1 14 1 1302.2 0 1 15 1 1302.2 0 1 17 2 1302.2 0 2 18 5 1302.2 0 5 19 2 1302.2 0 2 20 2 1302.2 0 2 21 2 1302.2 0 2 22 5 1302.2 0 5 23 3 1302.2 0 3 24 4 1302.2 0 4 25 4 1302.2 0 4 26 1 1302.2 0 1 27 2 1302.2 0 2 28 6 1302.2 0 6 29 5 1302.2 0 5 30 10 1302.2 0 10 31 18 1302.2 0 18 32 30 1302.2 0 30 33 27 1302.2 0 27 34 46 1302.2 0 46 35 5 1302.2 0 5 36 3 1302.2 0 3 37 18 1302.2 0 18 38 131 1302.2 0 131 39 10 1302.2 0 10 40 3 1302.2 0 3 41 7 1302.2 0 7 42 5 1302.2 0 5 43 3 1302.2 0 3 44 1 1302.2 0 1 45 4 1302.2 0 4 46 1 1302.2 0 1 47 1 1302.2 0 1 48 4 1302.2 0 4 49 6 1302.2 0 6 50 2 1302.2 0 2 51 4 1302.2 0 4 52 3 1302.2 0 3 53 5 1302.2 0 5 54 7 1302.2 0 7 55 11 1302.2 0 11 56 19 1302.2 0 19 57 14 1302.2 0 14 58 13 1302.2 0 13 59 14 1302.2 0 14 60 146 1302.2 0 146 61 91 1302.2 0 91 62 97 1302.2 0 97 63 3 1302.2 0 3 64 4 1302.2 0 4 65 4 1302.2 0 4 66 29 1302.2 0 29 67 18 1302.2 0 18 68 16 1302.2 0 16 69 2 1302.2 0 2 70 13 1302.2 0 13 71 46 1302.2 0 46 72 7 1302.2 0 7 73 8 1302.2 0 8 74 6 1302.2 0 6 75 5 1302.2 0 5 76 4 1302.2 0 4 77 47 1302.2 0 47 78 6 1302.2 0 6 79 90 1302.2 0 90 80 19 1302.2 0 19 81 20 1302.2 0 20 82 66 1302.2 0 66 83 21 1302.2 0 21 84 27 1302.2 0 27 85 11 1302.2 0 11 86 24 1302.2 0 24 87 31 1302.2 0 31 88 8 1302.2 0 8 89 15 1302.2 0 15 90 6 1302.2 0 6 91 26 1302.2 0 26 92 72 1302.2 0 72 93 56 1302.2 0 56 94 50 1302.2 0 50 95 63 1302.2 0 63 96 94 1302.2 0 94 97 929 1302.2 0 929 98 173 1302.2 0 173 99 276 1302.2 0 276 100 25 1302.2 0 25 101 28 1302.2 0 28 102 38 1302.2 0 38 103 144 1302.2 0 144 104 49 1302.2 0 49 105 46 1302.2 0 46 106 30 1302.2 0 30 107 29 1302.2 0 29 108 73 1302.2 0 73 109 107 1302.2 0 107 110 20 1302.2 0 20 111 106 1302.2 0 106 112 183 1302.2 0 183 113 337 1302.2 0 337 114 298 1302.2 0 298 115 3039 1302.2 0 3039 116 912 1302.2 0 912 117 598 1302.2 0 598 118 56 1302.2 0 56 119 53 1302.2 0 53 120 118 1302.2 0 118 121 141 1302.2 0 141 122 118 1302.2 0 118 123 121 1302.2 0 121 124 125 1302.2 0 125 125 125 1302.2 0 125 126 97 1302.2 0 97 127 148 1302.2 0 148 128 133 1302.2 0 133 129 120 1302.2 0 120 130 148 1302.2 0 148 131 59 1302.2 0 59 132 90 1302.2 0 90 133 148 1302.2 0 148 134 155 1302.2 0 155 135 169 1302.2 0 169 136 176 1302.2 0 176 137 254 1302.2 0 254 138 177 1302.2 0 177 139 246 1302.2 0 246 140 104 1302.2 0 104 141 163 1302.2 0 163 142 143 1302.2 0 143 143 197 1302.2 0 197 144 175 1302.2 0 175 145 100 1302.2 0 100 146 153 1302.2 0 153 147 116 1302.2 0 116 148 134 1302.2 0 134 149 94 1302.2 0 94 150 112 1302.2 0 112 151 125 1302.2 0 125 152 80 1302.2 0 80 153 65 1302.2 0 65 154 98 1302.2 0 98 155 136 1302.2 0 136 156 217 1302.2 0 217 157 143 1302.2 0 143 158 100 1302.2 0 100 159 203 1302.2 0 203 160 130 1302.2 0 130 161 368 1302.2 0 368 162 191 1302.2 0 191 163 237 1302.2 0 237 164 237 1302.2 0 237 165 284 1302.2 0 284 166 451 1302.2 0 451 167 387 1302.2 0 387 168 250 1302.2 0 250 169 189 1302.2 0 189 170 351 1302.2 0 351 171 189 1302.2 0 189 172 337 1302.2 0 337 173 141 1302.2 0 141 174 89 1302.2 0 89 175 148 1302.2 0 148 176 234 1302.2 0 234 177 240 1302.2 0 240 178 157 1302.2 0 157 179 326 1302.2 0 326 180 121 1302.2 0 121 181 279 1302.2 0 279 182 317 1302.2 0 317 183 283 1302.2 0 283 184 316 1302.2 0 316 185 299 1302.2 0 299 186 596 1302.2 0 596 187 249 1302.2 0 249 188 198 1302.2 0 198 189 315 1302.2 0 315 190 519 1302.2 0 519 191 293 1302.2 0 293 192 271 1302.2 0 271 193 253 1302.2 0 253 194 233 1302.2 0 233 195 201 1302.2 0 201 196 330 1302.2 0 330 197 252 1302.2 0 252 198 167 1302.2 0 167 199 158 1302.2 0 158 200 200 1302.2 0 200 201 345 1302.2 0 345 202 264 1302.2 0 264 203 245 1302.2 0 245 204 316 1302.2 0 316 205 258 1302.2 0 258 206 224 1302.2 0 224 207 191 1302.2 0 191 208 265 1302.2 0 265 209 258 1302.2 0 258 210 166 1302.2 0 166 211 278 1302.2 0 278 212 299 1302.2 0 299 213 307 1302.2 0 307 214 326 1302.2 0 326 215 271 1302.2 0 271 216 369 1302.2 0 369 217 483 1302.2 0 483 218 341 1302.2 0 341 219 337 1302.2 0 337 220 465 1302.2 0 465 221 228 1302.2 0 228 222 258 1302.2 0 258 223 146 1302.2 0 146 224 104 1302.2 0 104 225 317 1302.2 0 317 226 363 1302.2 0 363 227 330 1302.2 0 330 228 390 1302.2 0 390 229 317 1302.2 0 317 230 276 1302.2 0 276 231 291 1302.2 0 291 232 345 1302.2 0 345 233 331 1302.2 0 331 234 279 1302.2 0 279 235 240 1302.2 0 240 236 711 1302.2 0 711 237 657 1302.2 0 657 238 472 1302.2 0 472 239 321 1302.2 0 321 240 217 1302.2 0 217 241 160 1302.2 0 160 242 151 1302.2 0 151 243 161 1302.2 0 161 244 144 1302.2 0 144 245 1017 1302.2 0 1017 246 2373 1302.2 0 2373 247 2890 1302.2 0 2890 248 251 1302.2 0 251 249 297 1302.2 0 297 250 163 1302.2 0 163 251 116 1302.2 0 116 252 96 1302.2 0 96 253 142 1302.2 0 142 254 346 1302.2 0 346 255 569 1302.2 0 569 256 641 1302.2 0 641 257 381 1302.2 0 381 258 311 1302.2 0 311 259 427 1302.2 0 427 260 557 1302.2 0 557 261 376 1302.2 0 376 262 327 1302.2 0 327 263 201 1302.2 0 201 264 496 1302.2 0 496 265 603 1302.2 0 603 266 1216 1302.2 0 1216 267 1461 1302.2 0 1461 268 402 1302.2 0 402 269 411 1302.2 0 411 270 424 1302.2 0 424 271 261 1302.2 0 261 272 192 1302.2 0 192 273 171 1302.2 0 171 274 174 1302.2 0 174 275 249 1302.2 0 249 276 326 1302.2 0 326 277 343 1302.2 0 343 278 321 1302.2 0 321 279 209 1302.2 0 209 280 332 1302.2 0 332 281 230 1302.2 0 230 282 428 1302.2 0 428 283 322 1302.2 0 322 284 325 1302.2 0 325 285 384 1302.2 0 384 286 477 1302.2 0 477 287 455 1302.2 0 455 288 466 1302.2 0 466 289 431 1302.2 0 431 290 319 1302.2 0 319 291 201 1302.2 0 201 292 137 1302.2 0 137 293 272 1302.2 0 272 294 327 1302.2 0 327 295 755 1302.2 0 755 296 455 1302.2 0 455 297 211 1302.2 0 211 298 172 1302.2 0 172 299 155 1302.2 0 155 300 118 1302.2 0 118 301 44 1302.2 0 44 Finished in 6.24 s (53 us/read; 1.13 M reads/minute). === Summary === Total reads processed: 117,732 Reads with adapters: 63,588 (54.0%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 63,571 (54.0%) Total basepairs processed: 30,905,861 bp Total written (filtered): 17,290,123 bp (55.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 63588 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 8 0.0 3 0 0 0 1 7 18 25 0.0 3 0 0 24 1 19 977 0.0 3 0 943 20 2 12 20 62136 0.0 4 61187 914 26 6 3 21 155 0.0 4 35 110 4 6 22 188 0.0 4 164 3 15 1 5 23 19 0.0 4 1 1 0 15 2 24 6 0.0 4 0 0 0 0 6 26 1 0.0 4 0 0 0 0 1 29 1 0.0 4 1 32 1 0.0 4 1 37 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 49 0.0 4 0 0 0 0 49 129 1 0.0 4 0 0 0 1 130 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 248 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.67 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 63,571 Reads with adapters: 2,896 (4.6%) Reads written (passing filters): 63,571 (100.0%) Total basepairs processed: 17,290,123 bp Total written (filtered): 16,719,306 bp (96.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2896 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 74.7% C: 1.7% G: 23.0% T: 0.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 993.3 0 2 4 6 248.3 0 6 5 1 62.1 0 1 9 52 0.2 0 2 50 10 458 0.1 1 17 441 11 184 0.0 1 0 184 208 1 0.0 1 1 256 5 0.0 1 5 257 2071 0.0 1 1914 157 258 91 0.0 1 87 4 259 14 0.0 1 12 2 260 9 0.0 1 8 1 261 1 0.0 1 1 270 1 0.0 1 1