This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 180.25 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 962,551 (18.0%) Reads that were too short: 459,291 (8.6%) Reads written (passing filters): 503,260 (9.4%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 81,625,157 bp (5.1%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 962551 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 118 1302.2 0 118 7 1808 1302.2 0 1808 8 2956 1302.2 0 2956 9 125 1302.2 0 125 10 359 1302.2 0 359 11 14067 1302.2 0 14067 12 35 1302.2 0 35 13 18 1302.2 0 18 14 17 1302.2 0 17 15 17 1302.2 0 17 16 26 1302.2 0 26 17 19 1302.2 0 19 18 18 1302.2 0 18 19 16 1302.2 0 16 20 25 1302.2 0 25 21 19 1302.2 0 19 22 26 1302.2 0 26 23 17 1302.2 0 17 24 23 1302.2 0 23 25 27 1302.2 0 27 26 22 1302.2 0 22 27 14 1302.2 0 14 28 11 1302.2 0 11 29 20 1302.2 0 20 30 26 1302.2 0 26 31 22 1302.2 0 22 32 20 1302.2 0 20 33 15 1302.2 0 15 34 25 1302.2 0 25 35 24 1302.2 0 24 36 28 1302.2 0 28 37 15 1302.2 0 15 38 30 1302.2 0 30 39 25 1302.2 0 25 40 17 1302.2 0 17 41 21 1302.2 0 21 42 20 1302.2 0 20 43 23 1302.2 0 23 44 16 1302.2 0 16 45 21 1302.2 0 21 46 15 1302.2 0 15 47 16 1302.2 0 16 48 18 1302.2 0 18 49 18 1302.2 0 18 50 23 1302.2 0 23 51 18 1302.2 0 18 52 30 1302.2 0 30 53 29 1302.2 0 29 54 36 1302.2 0 36 55 21 1302.2 0 21 56 18 1302.2 0 18 57 16 1302.2 0 16 58 20 1302.2 0 20 59 18 1302.2 0 18 60 22 1302.2 0 22 61 18 1302.2 0 18 62 14 1302.2 0 14 63 28 1302.2 0 28 64 21 1302.2 0 21 65 16 1302.2 0 16 66 19 1302.2 0 19 67 15 1302.2 0 15 68 30 1302.2 0 30 69 27 1302.2 0 27 70 30 1302.2 0 30 71 41 1302.2 0 41 72 74 1302.2 0 74 73 45 1302.2 0 45 74 268 1302.2 0 268 75 153 1302.2 0 153 76 119 1302.2 0 119 77 548 1302.2 0 548 78 1990 1302.2 0 1990 79 2253 1302.2 0 2253 80 4609 1302.2 0 4609 81 2944 1302.2 0 2944 82 2134 1302.2 0 2134 83 3114 1302.2 0 3114 84 7554 1302.2 0 7554 85 1618 1302.2 0 1618 86 2232 1302.2 0 2232 87 5878 1302.2 0 5878 88 9990 1302.2 0 9990 89 497 1302.2 0 497 90 951 1302.2 0 951 91 10081 1302.2 0 10081 92 3204 1302.2 0 3204 93 7061 1302.2 0 7061 94 1713 1302.2 0 1713 95 2252 1302.2 0 2252 96 744 1302.2 0 744 97 1244 1302.2 0 1244 98 1867 1302.2 0 1867 99 1411 1302.2 0 1411 100 985 1302.2 0 985 101 3105 1302.2 0 3105 102 1193 1302.2 0 1193 103 2491 1302.2 0 2491 104 8356 1302.2 0 8356 105 3100 1302.2 0 3100 106 1203 1302.2 0 1203 107 2584 1302.2 0 2584 108 2276 1302.2 0 2276 109 1666 1302.2 0 1666 110 1480 1302.2 0 1480 111 2001 1302.2 0 2001 112 1292 1302.2 0 1292 113 2296 1302.2 0 2296 114 1540 1302.2 0 1540 115 1704 1302.2 0 1704 116 2529 1302.2 0 2529 117 6553 1302.2 0 6553 118 1814 1302.2 0 1814 119 3478 1302.2 0 3478 120 3358 1302.2 0 3358 121 3305 1302.2 0 3305 122 3795 1302.2 0 3795 123 5347 1302.2 0 5347 124 5680 1302.2 0 5680 125 4521 1302.2 0 4521 126 3982 1302.2 0 3982 127 1660 1302.2 0 1660 128 2136 1302.2 0 2136 129 3645 1302.2 0 3645 130 6671 1302.2 0 6671 131 3900 1302.2 0 3900 132 7936 1302.2 0 7936 133 3951 1302.2 0 3951 134 4236 1302.2 0 4236 135 3360 1302.2 0 3360 136 3313 1302.2 0 3313 137 3822 1302.2 0 3822 138 3005 1302.2 0 3005 139 3247 1302.2 0 3247 140 1429 1302.2 0 1429 141 2724 1302.2 0 2724 142 4376 1302.2 0 4376 143 7634 1302.2 0 7634 144 2701 1302.2 0 2701 145 3476 1302.2 0 3476 146 3318 1302.2 0 3318 147 3111 1302.2 0 3111 148 3512 1302.2 0 3512 149 3435 1302.2 0 3435 150 4733 1302.2 0 4733 151 4240 1302.2 0 4240 152 5215 1302.2 0 5215 153 3484 1302.2 0 3484 154 3900 1302.2 0 3900 155 6109 1302.2 0 6109 156 4970 1302.2 0 4970 157 5212 1302.2 0 5212 158 6623 1302.2 0 6623 159 3527 1302.2 0 3527 160 3995 1302.2 0 3995 161 3680 1302.2 0 3680 162 4857 1302.2 0 4857 163 5316 1302.2 0 5316 164 3935 1302.2 0 3935 165 3279 1302.2 0 3279 166 3261 1302.2 0 3261 167 4346 1302.2 0 4346 168 3833 1302.2 0 3833 169 3703 1302.2 0 3703 170 7333 1302.2 0 7333 171 10668 1302.2 0 10668 172 6421 1302.2 0 6421 173 2507 1302.2 0 2507 174 3432 1302.2 0 3432 175 2718 1302.2 0 2718 176 3737 1302.2 0 3737 177 3993 1302.2 0 3993 178 7176 1302.2 0 7176 179 6832 1302.2 0 6832 180 5093 1302.2 0 5093 181 3491 1302.2 0 3491 182 4113 1302.2 0 4113 183 4984 1302.2 0 4984 184 3453 1302.2 0 3453 185 2402 1302.2 0 2402 186 1814 1302.2 0 1814 187 3892 1302.2 0 3892 188 7917 1302.2 0 7917 189 8244 1302.2 0 8244 190 5414 1302.2 0 5414 191 3826 1302.2 0 3826 192 3806 1302.2 0 3806 193 2613 1302.2 0 2613 194 3278 1302.2 0 3278 195 10314 1302.2 0 10314 196 4785 1302.2 0 4785 197 1617 1302.2 0 1617 198 2751 1302.2 0 2751 199 2391 1302.2 0 2391 200 3559 1302.2 0 3559 201 4960 1302.2 0 4960 202 3098 1302.2 0 3098 203 2848 1302.2 0 2848 204 3187 1302.2 0 3187 205 2755 1302.2 0 2755 206 1603 1302.2 0 1603 207 2109 1302.2 0 2109 208 2747 1302.2 0 2747 209 2609 1302.2 0 2609 210 2536 1302.2 0 2536 211 2783 1302.2 0 2783 212 2651 1302.2 0 2651 213 3177 1302.2 0 3177 214 5094 1302.2 0 5094 215 5807 1302.2 0 5807 216 5816 1302.2 0 5816 217 5385 1302.2 0 5385 218 3788 1302.2 0 3788 219 2121 1302.2 0 2121 220 1972 1302.2 0 1972 221 5707 1302.2 0 5707 222 5360 1302.2 0 5360 223 4043 1302.2 0 4043 224 5255 1302.2 0 5255 225 5869 1302.2 0 5869 226 8938 1302.2 0 8938 227 13354 1302.2 0 13354 228 8310 1302.2 0 8310 229 8344 1302.2 0 8344 230 5511 1302.2 0 5511 231 6136 1302.2 0 6136 232 9601 1302.2 0 9601 233 8802 1302.2 0 8802 234 2738 1302.2 0 2738 235 2249 1302.2 0 2249 236 2842 1302.2 0 2842 237 2776 1302.2 0 2776 238 2514 1302.2 0 2514 239 3104 1302.2 0 3104 240 2856 1302.2 0 2856 241 3522 1302.2 0 3522 242 4301 1302.2 0 4301 243 3469 1302.2 0 3469 244 3383 1302.2 0 3383 245 2652 1302.2 0 2652 246 1826 1302.2 0 1826 247 2219 1302.2 0 2219 248 5707 1302.2 0 5707 249 12494 1302.2 0 12494 250 11928 1302.2 0 11928 251 6001 1302.2 0 6001 252 5504 1302.2 0 5504 253 7362 1302.2 0 7362 254 4737 1302.2 0 4737 255 3800 1302.2 0 3800 256 2373 1302.2 0 2373 257 3074 1302.2 0 3074 258 2913 1302.2 0 2913 259 4148 1302.2 0 4148 260 3922 1302.2 0 3922 261 5333 1302.2 0 5333 262 4359 1302.2 0 4359 263 5229 1302.2 0 5229 264 6902 1302.2 0 6902 265 5113 1302.2 0 5113 266 3349 1302.2 0 3349 267 2036 1302.2 0 2036 268 2601 1302.2 0 2601 269 3828 1302.2 0 3828 270 3776 1302.2 0 3776 271 5886 1302.2 0 5886 272 5981 1302.2 0 5981 273 6038 1302.2 0 6038 274 7354 1302.2 0 7354 275 6714 1302.2 0 6714 276 8005 1302.2 0 8005 277 6396 1302.2 0 6396 278 4437 1302.2 0 4437 279 3348 1302.2 0 3348 280 4055 1302.2 0 4055 281 7918 1302.2 0 7918 282 6590 1302.2 0 6590 283 3712 1302.2 0 3712 284 5745 1302.2 0 5745 285 3760 1302.2 0 3760 286 2362 1302.2 0 2362 287 1829 1302.2 0 1829 288 1939 1302.2 0 1939 289 3729 1302.2 0 3729 290 4564 1302.2 0 4564 291 7426 1302.2 0 7426 292 8721 1302.2 0 8721 293 7458 1302.2 0 7458 294 1406 1302.2 0 1406 295 1459 1302.2 0 1459 296 1647 1302.2 0 1647 297 3325 1302.2 0 3325 298 4367 1302.2 0 4367 299 4061 1302.2 0 4061 300 2967 1302.2 0 2967 301 1836 1302.2 0 1836 Finished in 21.10 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 503,260 Reads with adapters: 3,109 (0.6%) Reads that were too short: 110 (0.0%) Reads written (passing filters): 2,999 (0.6%) Total basepairs processed: 81,625,157 bp Total written (filtered): 812,133 bp (1.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3109 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 1 1 19 43 0.0 3 0 34 0 3 6 20 2880 0.0 4 2833 45 1 0 1 21 14 0.0 4 4 6 0 2 2 22 9 0.0 4 1 0 0 1 7 23 7 0.0 4 4 0 0 3 24 1 0.0 4 0 0 0 0 1 25 3 0.0 4 0 0 0 0 3 28 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 1 31 1 0.0 4 0 0 0 0 1 33 2 0.0 4 0 0 0 0 2 34 2 0.0 4 0 0 0 0 2 35 1 0.0 4 0 0 0 0 1 36 3 0.0 4 0 0 0 0 3 37 1 0.0 4 0 0 0 1 38 2 0.0 4 0 0 0 0 2 39 1 0.0 4 0 0 0 0 1 40 2 0.0 4 0 0 0 0 2 41 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 46 2 0.0 4 0 0 0 0 2 47 3 0.0 4 0 0 0 0 3 48 2 0.0 4 0 0 0 0 2 49 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 51 2 0.0 4 0 0 0 0 2 52 2 0.0 4 0 0 0 0 2 55 1 0.0 4 0 0 0 0 1 56 2 0.0 4 0 0 0 0 2 57 1 0.0 4 0 0 0 0 1 59 5 0.0 4 0 0 0 0 5 61 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 63 2 0.0 4 0 0 0 0 2 64 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 66 2 0.0 4 0 0 0 0 2 67 1 0.0 4 0 0 0 1 69 2 0.0 4 0 0 0 0 2 70 2 0.0 4 0 0 0 0 2 71 1 0.0 4 0 0 0 0 1 74 3 0.0 4 0 0 0 0 3 76 4 0.0 4 0 0 0 0 4 78 1 0.0 4 0 0 0 0 1 79 4 0.0 4 0 0 0 1 3 81 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 88 2 0.0 4 0 0 0 0 2 90 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 93 3 0.0 4 0 0 0 0 3 94 2 0.0 4 0 0 0 0 2 96 2 0.0 4 0 0 0 0 2 97 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 0 2 109 3 0.0 4 0 0 0 0 3 112 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 115 2 0.0 4 0 0 0 0 2 116 1 0.0 4 0 0 0 0 1 118 3 0.0 4 0 0 0 0 3 121 1 0.0 4 0 0 0 0 1 122 2 0.0 4 0 0 0 0 2 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 7 0.0 4 0 0 0 0 7 126 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 129 2 0.0 4 0 0 0 0 2 132 2 0.0 4 0 0 0 0 2 133 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 140 2 0.0 4 0 0 0 0 2 142 1 0.0 4 0 0 0 0 1 143 2 0.0 4 0 0 0 0 2 144 1 0.0 4 0 0 0 0 1 145 2 0.0 4 0 0 0 0 2 146 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 1 204 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 226 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.13 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 2,999 Reads with adapters: 98 (3.3%) Reads written (passing filters): 2,999 (100.0%) Total basepairs processed: 812,133 bp Total written (filtered): 810,276 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 98 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 0.0% G: 95.9% T: 2.0% none/other: 1.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 10 1 0.0 1 0 1 11 94 0.0 1 0 94 268 1 0.0 1 1 272 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.