This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 172.51 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 336,129 (6.3%) Reads that were too short: 127,973 (2.4%) Reads written (passing filters): 208,156 (3.9%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 38,739,090 bp (2.4%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 336129 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 617 1302.2 0 617 7 3765 1302.2 0 3765 8 569 1302.2 0 569 9 35673 1302.2 0 35673 10 62 1302.2 0 62 11 8 1302.2 0 8 12 11 1302.2 0 11 13 14 1302.2 0 14 14 73 1302.2 0 73 15 17 1302.2 0 17 16 15 1302.2 0 15 17 31 1302.2 0 31 18 15 1302.2 0 15 19 16 1302.2 0 16 20 12 1302.2 0 12 21 12 1302.2 0 12 22 10 1302.2 0 10 23 17 1302.2 0 17 24 8 1302.2 0 8 25 7 1302.2 0 7 26 22 1302.2 0 22 27 14 1302.2 0 14 28 15 1302.2 0 15 29 19 1302.2 0 19 30 7 1302.2 0 7 31 25 1302.2 0 25 32 27 1302.2 0 27 33 17 1302.2 0 17 34 26 1302.2 0 26 35 25 1302.2 0 25 36 74 1302.2 0 74 37 34 1302.2 0 34 38 59 1302.2 0 59 39 27 1302.2 0 27 40 23 1302.2 0 23 41 14 1302.2 0 14 42 20 1302.2 0 20 43 14 1302.2 0 14 44 21 1302.2 0 21 45 18 1302.2 0 18 46 8 1302.2 0 8 47 16 1302.2 0 16 48 24 1302.2 0 24 49 5 1302.2 0 5 50 10 1302.2 0 10 51 21 1302.2 0 21 52 13 1302.2 0 13 53 10 1302.2 0 10 54 16 1302.2 0 16 55 10 1302.2 0 10 56 24 1302.2 0 24 57 125 1302.2 0 125 58 38 1302.2 0 38 59 9 1302.2 0 9 60 11 1302.2 0 11 61 32 1302.2 0 32 62 38 1302.2 0 38 63 14 1302.2 0 14 64 14 1302.2 0 14 65 14 1302.2 0 14 66 17 1302.2 0 17 67 46 1302.2 0 46 68 21 1302.2 0 21 69 9 1302.2 0 9 70 27 1302.2 0 27 71 61 1302.2 0 61 72 57 1302.2 0 57 73 76 1302.2 0 76 74 72 1302.2 0 72 75 60 1302.2 0 60 76 330 1302.2 0 330 77 89 1302.2 0 89 78 240 1302.2 0 240 79 762 1302.2 0 762 80 422 1302.2 0 422 81 459 1302.2 0 459 82 772 1302.2 0 772 83 1258 1302.2 0 1258 84 427 1302.2 0 427 85 733 1302.2 0 733 86 628 1302.2 0 628 87 2783 1302.2 0 2783 88 1094 1302.2 0 1094 89 4219 1302.2 0 4219 90 1194 1302.2 0 1194 91 1171 1302.2 0 1171 92 719 1302.2 0 719 93 680 1302.2 0 680 94 635 1302.2 0 635 95 309 1302.2 0 309 96 101 1302.2 0 101 97 340 1302.2 0 340 98 336 1302.2 0 336 99 674 1302.2 0 674 100 1792 1302.2 0 1792 101 533 1302.2 0 533 102 573 1302.2 0 573 103 1154 1302.2 0 1154 104 1307 1302.2 0 1307 105 1516 1302.2 0 1516 106 597 1302.2 0 597 107 276 1302.2 0 276 108 336 1302.2 0 336 109 997 1302.2 0 997 110 165 1302.2 0 165 111 286 1302.2 0 286 112 663 1302.2 0 663 113 708 1302.2 0 708 114 394 1302.2 0 394 115 382 1302.2 0 382 116 592 1302.2 0 592 117 828 1302.2 0 828 118 542 1302.2 0 542 119 831 1302.2 0 831 120 1289 1302.2 0 1289 121 711 1302.2 0 711 122 1267 1302.2 0 1267 123 459 1302.2 0 459 124 340 1302.2 0 340 125 516 1302.2 0 516 126 688 1302.2 0 688 127 349 1302.2 0 349 128 378 1302.2 0 378 129 611 1302.2 0 611 130 808 1302.2 0 808 131 878 1302.2 0 878 132 476 1302.2 0 476 133 918 1302.2 0 918 134 1541 1302.2 0 1541 135 1894 1302.2 0 1894 136 769 1302.2 0 769 137 928 1302.2 0 928 138 1056 1302.2 0 1056 139 1092 1302.2 0 1092 140 7057 1302.2 0 7057 141 2874 1302.2 0 2874 142 2961 1302.2 0 2961 143 659 1302.2 0 659 144 1432 1302.2 0 1432 145 1629 1302.2 0 1629 146 17784 1302.2 0 17784 147 6013 1302.2 0 6013 148 9639 1302.2 0 9639 149 993 1302.2 0 993 150 7538 1302.2 0 7538 151 2798 1302.2 0 2798 152 4993 1302.2 0 4993 153 661 1302.2 0 661 154 713 1302.2 0 713 155 734 1302.2 0 734 156 5201 1302.2 0 5201 157 2507 1302.2 0 2507 158 2178 1302.2 0 2178 159 1027 1302.2 0 1027 160 1629 1302.2 0 1629 161 1487 1302.2 0 1487 162 1309 1302.2 0 1309 163 821 1302.2 0 821 164 1137 1302.2 0 1137 165 918 1302.2 0 918 166 1299 1302.2 0 1299 167 894 1302.2 0 894 168 1172 1302.2 0 1172 169 879 1302.2 0 879 170 555 1302.2 0 555 171 367 1302.2 0 367 172 335 1302.2 0 335 173 705 1302.2 0 705 174 229 1302.2 0 229 175 294 1302.2 0 294 176 299 1302.2 0 299 177 454 1302.2 0 454 178 571 1302.2 0 571 179 1072 1302.2 0 1072 180 984 1302.2 0 984 181 1516 1302.2 0 1516 182 2232 1302.2 0 2232 183 1469 1302.2 0 1469 184 1428 1302.2 0 1428 185 1441 1302.2 0 1441 186 1310 1302.2 0 1310 187 394 1302.2 0 394 188 508 1302.2 0 508 189 611 1302.2 0 611 190 744 1302.2 0 744 191 889 1302.2 0 889 192 502 1302.2 0 502 193 391 1302.2 0 391 194 327 1302.2 0 327 195 429 1302.2 0 429 196 405 1302.2 0 405 197 1089 1302.2 0 1089 198 509 1302.2 0 509 199 189 1302.2 0 189 200 223 1302.2 0 223 201 583 1302.2 0 583 202 1469 1302.2 0 1469 203 1657 1302.2 0 1657 204 1219 1302.2 0 1219 205 1168 1302.2 0 1168 206 948 1302.2 0 948 207 2375 1302.2 0 2375 208 2755 1302.2 0 2755 209 1870 1302.2 0 1870 210 1999 1302.2 0 1999 211 789 1302.2 0 789 212 1343 1302.2 0 1343 213 1286 1302.2 0 1286 214 1072 1302.2 0 1072 215 949 1302.2 0 949 216 1325 1302.2 0 1325 217 1293 1302.2 0 1293 218 692 1302.2 0 692 219 566 1302.2 0 566 220 1429 1302.2 0 1429 221 3091 1302.2 0 3091 222 3887 1302.2 0 3887 223 1951 1302.2 0 1951 224 1611 1302.2 0 1611 225 1813 1302.2 0 1813 226 1084 1302.2 0 1084 227 856 1302.2 0 856 228 1153 1302.2 0 1153 229 492 1302.2 0 492 230 419 1302.2 0 419 231 621 1302.2 0 621 232 1033 1302.2 0 1033 233 851 1302.2 0 851 234 1179 1302.2 0 1179 235 1248 1302.2 0 1248 236 545 1302.2 0 545 237 441 1302.2 0 441 238 301 1302.2 0 301 239 218 1302.2 0 218 240 290 1302.2 0 290 241 862 1302.2 0 862 242 1175 1302.2 0 1175 243 2183 1302.2 0 2183 244 1279 1302.2 0 1279 245 813 1302.2 0 813 246 515 1302.2 0 515 247 506 1302.2 0 506 248 310 1302.2 0 310 249 258 1302.2 0 258 250 518 1302.2 0 518 251 1608 1302.2 0 1608 252 2032 1302.2 0 2032 253 1017 1302.2 0 1017 254 1972 1302.2 0 1972 255 5045 1302.2 0 5045 256 19238 1302.2 0 19238 257 3374 1302.2 0 3374 258 2810 1302.2 0 2810 259 587 1302.2 0 587 260 497 1302.2 0 497 261 414 1302.2 0 414 262 718 1302.2 0 718 263 1290 1302.2 0 1290 264 1340 1302.2 0 1340 265 1359 1302.2 0 1359 266 1929 1302.2 0 1929 267 996 1302.2 0 996 268 455 1302.2 0 455 269 253 1302.2 0 253 270 789 1302.2 0 789 271 802 1302.2 0 802 272 609 1302.2 0 609 273 458 1302.2 0 458 274 354 1302.2 0 354 275 360 1302.2 0 360 276 371 1302.2 0 371 277 583 1302.2 0 583 278 787 1302.2 0 787 279 993 1302.2 0 993 280 974 1302.2 0 974 281 646 1302.2 0 646 282 860 1302.2 0 860 283 1370 1302.2 0 1370 284 1130 1302.2 0 1130 285 628 1302.2 0 628 286 556 1302.2 0 556 287 875 1302.2 0 875 288 429 1302.2 0 429 289 328 1302.2 0 328 290 854 1302.2 0 854 291 874 1302.2 0 874 292 987 1302.2 0 987 293 938 1302.2 0 938 294 435 1302.2 0 435 295 381 1302.2 0 381 296 759 1302.2 0 759 297 408 1302.2 0 408 298 1160 1302.2 0 1160 299 1984 1302.2 0 1984 300 582 1302.2 0 582 301 68 1302.2 0 68 Finished in 8.51 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 208,156 Reads with adapters: 36,391 (17.5%) Reads that were too short: 28 (0.0%) Reads written (passing filters): 36,363 (17.5%) Total basepairs processed: 38,739,090 bp Total written (filtered): 9,996,155 bp (25.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 36391 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 10 0.0 3 0 0 7 3 16 354 0.0 3 0 321 19 14 17 35765 0.0 3 34446 1147 124 48 18 98 0.0 3 15 58 17 8 19 17 0.0 3 0 0 8 9 20 83 0.0 3 0 1 0 82 23 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 1 31 1 0.0 3 1 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 3 0.0 3 0 0 1 2 107 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 144 4 0.0 3 0 0 0 4 145 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.64 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 36,363 Reads with adapters: 261 (0.7%) Reads written (passing filters): 36,363 (100.0%) Total basepairs processed: 9,996,155 bp Total written (filtered): 9,995,316 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 261 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 40.2% C: 6.9% G: 39.5% T: 13.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 205 568.2 0 205 4 56 142.0 0 56