This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 169.79 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 64,332 (1.2%) Reads that were too short: 22,870 (0.4%) Reads written (passing filters): 41,462 (0.8%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 8,662,910 bp (0.5%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 64332 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 279 1302.2 0 279 7 10982 1302.2 0 10982 8 90 1302.2 0 90 9 2758 1302.2 0 2758 10 10 1302.2 0 10 11 9 1302.2 0 9 12 8 1302.2 0 8 13 7 1302.2 0 7 14 6 1302.2 0 6 15 9 1302.2 0 9 16 43 1302.2 0 43 17 8 1302.2 0 8 18 8 1302.2 0 8 19 6 1302.2 0 6 20 7 1302.2 0 7 21 3 1302.2 0 3 22 8 1302.2 0 8 23 10 1302.2 0 10 24 3 1302.2 0 3 25 7 1302.2 0 7 26 7 1302.2 0 7 27 9 1302.2 0 9 28 111 1302.2 0 111 29 17 1302.2 0 17 30 12 1302.2 0 12 31 8 1302.2 0 8 32 8 1302.2 0 8 33 18 1302.2 0 18 34 6 1302.2 0 6 35 11 1302.2 0 11 36 7 1302.2 0 7 37 11 1302.2 0 11 38 7 1302.2 0 7 39 8 1302.2 0 8 40 6 1302.2 0 6 41 10 1302.2 0 10 42 8 1302.2 0 8 43 12 1302.2 0 12 44 279 1302.2 0 279 45 6 1302.2 0 6 46 51 1302.2 0 51 47 10 1302.2 0 10 48 799 1302.2 0 799 49 8 1302.2 0 8 50 11 1302.2 0 11 51 7 1302.2 0 7 52 11 1302.2 0 11 53 16 1302.2 0 16 54 9 1302.2 0 9 55 9 1302.2 0 9 56 29 1302.2 0 29 57 10 1302.2 0 10 58 434 1302.2 0 434 59 184 1302.2 0 184 60 13 1302.2 0 13 61 24 1302.2 0 24 62 9 1302.2 0 9 63 6 1302.2 0 6 64 7 1302.2 0 7 65 10 1302.2 0 10 66 8 1302.2 0 8 67 10 1302.2 0 10 68 11 1302.2 0 11 69 9 1302.2 0 9 70 10 1302.2 0 10 71 4 1302.2 0 4 72 4 1302.2 0 4 73 5 1302.2 0 5 74 4 1302.2 0 4 75 6 1302.2 0 6 76 7 1302.2 0 7 77 7 1302.2 0 7 78 3 1302.2 0 3 79 20 1302.2 0 20 80 13 1302.2 0 13 81 25 1302.2 0 25 82 41 1302.2 0 41 83 28 1302.2 0 28 84 42 1302.2 0 42 85 11 1302.2 0 11 86 13 1302.2 0 13 87 22 1302.2 0 22 88 21 1302.2 0 21 89 6 1302.2 0 6 90 8 1302.2 0 8 91 68 1302.2 0 68 92 96 1302.2 0 96 93 35 1302.2 0 35 94 70 1302.2 0 70 95 33 1302.2 0 33 96 41 1302.2 0 41 97 426 1302.2 0 426 98 351 1302.2 0 351 99 51 1302.2 0 51 100 16 1302.2 0 16 101 37 1302.2 0 37 102 37 1302.2 0 37 103 71 1302.2 0 71 104 245 1302.2 0 245 105 58 1302.2 0 58 106 571 1302.2 0 571 107 853 1302.2 0 853 108 1463 1302.2 0 1463 109 35 1302.2 0 35 110 52 1302.2 0 52 111 34 1302.2 0 34 112 39 1302.2 0 39 113 73 1302.2 0 73 114 147 1302.2 0 147 115 211 1302.2 0 211 116 416 1302.2 0 416 117 43 1302.2 0 43 118 41 1302.2 0 41 119 228 1302.2 0 228 120 118 1302.2 0 118 121 147 1302.2 0 147 122 67 1302.2 0 67 123 107 1302.2 0 107 124 83 1302.2 0 83 125 129 1302.2 0 129 126 79 1302.2 0 79 127 77 1302.2 0 77 128 204 1302.2 0 204 129 149 1302.2 0 149 130 137 1302.2 0 137 131 57 1302.2 0 57 132 62 1302.2 0 62 133 179 1302.2 0 179 134 384 1302.2 0 384 135 368 1302.2 0 368 136 263 1302.2 0 263 137 200 1302.2 0 200 138 363 1302.2 0 363 139 280 1302.2 0 280 140 286 1302.2 0 286 141 470 1302.2 0 470 142 950 1302.2 0 950 143 279 1302.2 0 279 144 77 1302.2 0 77 145 81 1302.2 0 81 146 98 1302.2 0 98 147 131 1302.2 0 131 148 1528 1302.2 0 1528 149 716 1302.2 0 716 150 1088 1302.2 0 1088 151 50 1302.2 0 50 152 58 1302.2 0 58 153 76 1302.2 0 76 154 83 1302.2 0 83 155 983 1302.2 0 983 156 234 1302.2 0 234 157 375 1302.2 0 375 158 85 1302.2 0 85 159 122 1302.2 0 122 160 109 1302.2 0 109 161 137 1302.2 0 137 162 200 1302.2 0 200 163 855 1302.2 0 855 164 375 1302.2 0 375 165 529 1302.2 0 529 166 219 1302.2 0 219 167 265 1302.2 0 265 168 183 1302.2 0 183 169 73 1302.2 0 73 170 63 1302.2 0 63 171 153 1302.2 0 153 172 74 1302.2 0 74 173 47 1302.2 0 47 174 64 1302.2 0 64 175 84 1302.2 0 84 176 66 1302.2 0 66 177 89 1302.2 0 89 178 58 1302.2 0 58 179 60 1302.2 0 60 180 302 1302.2 0 302 181 594 1302.2 0 594 182 513 1302.2 0 513 183 336 1302.2 0 336 184 252 1302.2 0 252 185 166 1302.2 0 166 186 100 1302.2 0 100 187 52 1302.2 0 52 188 96 1302.2 0 96 189 142 1302.2 0 142 190 85 1302.2 0 85 191 145 1302.2 0 145 192 141 1302.2 0 141 193 124 1302.2 0 124 194 109 1302.2 0 109 195 155 1302.2 0 155 196 118 1302.2 0 118 197 34 1302.2 0 34 198 28 1302.2 0 28 199 50 1302.2 0 50 200 148 1302.2 0 148 201 188 1302.2 0 188 202 171 1302.2 0 171 203 176 1302.2 0 176 204 257 1302.2 0 257 205 181 1302.2 0 181 206 128 1302.2 0 128 207 187 1302.2 0 187 208 143 1302.2 0 143 209 99 1302.2 0 99 210 160 1302.2 0 160 211 94 1302.2 0 94 212 135 1302.2 0 135 213 178 1302.2 0 178 214 227 1302.2 0 227 215 289 1302.2 0 289 216 267 1302.2 0 267 217 250 1302.2 0 250 218 154 1302.2 0 154 219 117 1302.2 0 117 220 176 1302.2 0 176 221 238 1302.2 0 238 222 81 1302.2 0 81 223 47 1302.2 0 47 224 130 1302.2 0 130 225 228 1302.2 0 228 226 389 1302.2 0 389 227 462 1302.2 0 462 228 270 1302.2 0 270 229 240 1302.2 0 240 230 96 1302.2 0 96 231 240 1302.2 0 240 232 537 1302.2 0 537 233 269 1302.2 0 269 234 168 1302.2 0 168 235 198 1302.2 0 198 236 232 1302.2 0 232 237 140 1302.2 0 140 238 108 1302.2 0 108 239 90 1302.2 0 90 240 104 1302.2 0 104 241 88 1302.2 0 88 242 85 1302.2 0 85 243 77 1302.2 0 77 244 163 1302.2 0 163 245 163 1302.2 0 163 246 147 1302.2 0 147 247 123 1302.2 0 123 248 126 1302.2 0 126 249 119 1302.2 0 119 250 137 1302.2 0 137 251 47 1302.2 0 47 252 109 1302.2 0 109 253 255 1302.2 0 255 254 713 1302.2 0 713 255 618 1302.2 0 618 256 392 1302.2 0 392 257 237 1302.2 0 237 258 314 1302.2 0 314 259 265 1302.2 0 265 260 219 1302.2 0 219 261 171 1302.2 0 171 262 95 1302.2 0 95 263 197 1302.2 0 197 264 258 1302.2 0 258 265 331 1302.2 0 331 266 331 1302.2 0 331 267 169 1302.2 0 169 268 232 1302.2 0 232 269 1030 1302.2 0 1030 270 469 1302.2 0 469 271 367 1302.2 0 367 272 212 1302.2 0 212 273 212 1302.2 0 212 274 109 1302.2 0 109 275 278 1302.2 0 278 276 317 1302.2 0 317 277 299 1302.2 0 299 278 228 1302.2 0 228 279 238 1302.2 0 238 280 264 1302.2 0 264 281 387 1302.2 0 387 282 236 1302.2 0 236 283 200 1302.2 0 200 284 290 1302.2 0 290 285 347 1302.2 0 347 286 218 1302.2 0 218 287 165 1302.2 0 165 288 175 1302.2 0 175 289 299 1302.2 0 299 290 189 1302.2 0 189 291 109 1302.2 0 109 292 126 1302.2 0 126 293 140 1302.2 0 140 294 478 1302.2 0 478 295 733 1302.2 0 733 296 378 1302.2 0 378 297 251 1302.2 0 251 298 167 1302.2 0 167 299 41 1302.2 0 41 300 57 1302.2 0 57 301 24 1302.2 0 24 Finished in 2.18 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 41,462 Reads with adapters: 2,836 (6.8%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 2,826 (6.8%) Total basepairs processed: 8,662,910 bp Total written (filtered): 766,414 bp (8.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2836 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 9 0.0 3 0 0 0 0 9 18 3 0.0 3 0 0 1 0 2 19 43 0.0 3 0 34 0 0 9 20 2714 0.0 4 2664 37 3 9 1 21 18 0.0 4 4 10 0 2 2 22 14 0.0 4 6 0 1 0 7 23 4 0.0 4 1 0 0 1 2 24 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 12 0.0 4 0 0 0 0 12 128 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 287 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (42 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 2,826 Reads with adapters: 45 (1.6%) Reads written (passing filters): 2,826 (100.0%) Total basepairs processed: 766,414 bp Total written (filtered): 764,967 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 45 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 13.3% C: 0.0% G: 84.4% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 6 1 0.7 0 1 9 5 0.0 0 0 5 10 25 0.0 1 0 25 11 10 0.0 1 0 10 257 3 0.0 1 2 1 265 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.