This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 174.47 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 2,978,740 (55.8%) Reads that were too short: 1,039,970 (19.5%) Reads written (passing filters): 1,938,770 (36.3%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 323,093,858 bp (20.1%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 2978740 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 208 1302.2 0 208 7 667 1302.2 0 667 8 343 1302.2 0 343 9 14209 1302.2 0 14209 10 55 1302.2 0 55 11 8 1302.2 0 8 12 2 1302.2 0 2 13 9 1302.2 0 9 14 10 1302.2 0 10 15 4 1302.2 0 4 16 10 1302.2 0 10 17 7 1302.2 0 7 18 8 1302.2 0 8 19 4 1302.2 0 4 20 3 1302.2 0 3 21 3 1302.2 0 3 22 12 1302.2 0 12 23 17 1302.2 0 17 24 10 1302.2 0 10 25 19 1302.2 0 19 26 6 1302.2 0 6 27 11 1302.2 0 11 28 12 1302.2 0 12 29 7 1302.2 0 7 30 14 1302.2 0 14 31 8 1302.2 0 8 32 8 1302.2 0 8 33 5 1302.2 0 5 34 6 1302.2 0 6 35 15 1302.2 0 15 36 8 1302.2 0 8 37 10 1302.2 0 10 38 5 1302.2 0 5 39 20 1302.2 0 20 40 20 1302.2 0 20 41 65 1302.2 0 65 42 102 1302.2 0 102 43 162 1302.2 0 162 44 17 1302.2 0 17 45 8 1302.2 0 8 46 5 1302.2 0 5 47 12 1302.2 0 12 48 9 1302.2 0 9 49 17 1302.2 0 17 50 20 1302.2 0 20 51 29 1302.2 0 29 52 64 1302.2 0 64 53 12 1302.2 0 12 54 7 1302.2 0 7 55 8 1302.2 0 8 56 13 1302.2 0 13 57 18 1302.2 0 18 58 16 1302.2 0 16 59 10 1302.2 0 10 60 16 1302.2 0 16 61 14 1302.2 0 14 62 230 1302.2 0 230 63 108 1302.2 0 108 64 146 1302.2 0 146 65 84 1302.2 0 84 66 364 1302.2 0 364 67 131 1302.2 0 131 68 337 1302.2 0 337 69 164 1302.2 0 164 70 343 1302.2 0 343 71 2038 1302.2 0 2038 72 8901 1302.2 0 8901 73 33224 1302.2 0 33224 74 9968 1302.2 0 9968 75 14788 1302.2 0 14788 76 7455 1302.2 0 7455 77 8650 1302.2 0 8650 78 14740 1302.2 0 14740 79 10273 1302.2 0 10273 80 15050 1302.2 0 15050 81 9009 1302.2 0 9009 82 8350 1302.2 0 8350 83 5821 1302.2 0 5821 84 5724 1302.2 0 5724 85 8314 1302.2 0 8314 86 9826 1302.2 0 9826 87 4204 1302.2 0 4204 88 5665 1302.2 0 5665 89 19600 1302.2 0 19600 90 9670 1302.2 0 9670 91 29778 1302.2 0 29778 92 9324 1302.2 0 9324 93 12510 1302.2 0 12510 94 12037 1302.2 0 12037 95 30828 1302.2 0 30828 96 13243 1302.2 0 13243 97 8685 1302.2 0 8685 98 4371 1302.2 0 4371 99 7419 1302.2 0 7419 100 7598 1302.2 0 7598 101 17182 1302.2 0 17182 102 16760 1302.2 0 16760 103 20272 1302.2 0 20272 104 13645 1302.2 0 13645 105 47112 1302.2 0 47112 106 42763 1302.2 0 42763 107 28810 1302.2 0 28810 108 14095 1302.2 0 14095 109 10700 1302.2 0 10700 110 9428 1302.2 0 9428 111 21217 1302.2 0 21217 112 18144 1302.2 0 18144 113 22034 1302.2 0 22034 114 20933 1302.2 0 20933 115 21348 1302.2 0 21348 116 8745 1302.2 0 8745 117 12135 1302.2 0 12135 118 12163 1302.2 0 12163 119 17371 1302.2 0 17371 120 16486 1302.2 0 16486 121 25751 1302.2 0 25751 122 13073 1302.2 0 13073 123 22533 1302.2 0 22533 124 18674 1302.2 0 18674 125 19273 1302.2 0 19273 126 23585 1302.2 0 23585 127 21091 1302.2 0 21091 128 15693 1302.2 0 15693 129 10547 1302.2 0 10547 130 14165 1302.2 0 14165 131 9744 1302.2 0 9744 132 16725 1302.2 0 16725 133 20010 1302.2 0 20010 134 20679 1302.2 0 20679 135 17317 1302.2 0 17317 136 13700 1302.2 0 13700 137 11329 1302.2 0 11329 138 10256 1302.2 0 10256 139 10577 1302.2 0 10577 140 12916 1302.2 0 12916 141 15913 1302.2 0 15913 142 10995 1302.2 0 10995 143 12879 1302.2 0 12879 144 11026 1302.2 0 11026 145 17047 1302.2 0 17047 146 17405 1302.2 0 17405 147 13466 1302.2 0 13466 148 20607 1302.2 0 20607 149 16635 1302.2 0 16635 150 19388 1302.2 0 19388 151 11182 1302.2 0 11182 152 15241 1302.2 0 15241 153 19936 1302.2 0 19936 154 18594 1302.2 0 18594 155 14910 1302.2 0 14910 156 12088 1302.2 0 12088 157 10784 1302.2 0 10784 158 13449 1302.2 0 13449 159 12198 1302.2 0 12198 160 13152 1302.2 0 13152 161 15335 1302.2 0 15335 162 18545 1302.2 0 18545 163 18471 1302.2 0 18471 164 17765 1302.2 0 17765 165 14463 1302.2 0 14463 166 12121 1302.2 0 12121 167 10212 1302.2 0 10212 168 10264 1302.2 0 10264 169 13113 1302.2 0 13113 170 13127 1302.2 0 13127 171 10297 1302.2 0 10297 172 16090 1302.2 0 16090 173 14478 1302.2 0 14478 174 27623 1302.2 0 27623 175 14179 1302.2 0 14179 176 11903 1302.2 0 11903 177 6993 1302.2 0 6993 178 11937 1302.2 0 11937 179 12005 1302.2 0 12005 180 14975 1302.2 0 14975 181 13021 1302.2 0 13021 182 10095 1302.2 0 10095 183 11613 1302.2 0 11613 184 11536 1302.2 0 11536 185 14488 1302.2 0 14488 186 18023 1302.2 0 18023 187 14396 1302.2 0 14396 188 12994 1302.2 0 12994 189 10670 1302.2 0 10670 190 13505 1302.2 0 13505 191 15986 1302.2 0 15986 192 16642 1302.2 0 16642 193 16543 1302.2 0 16543 194 14453 1302.2 0 14453 195 6606 1302.2 0 6606 196 7285 1302.2 0 7285 197 7975 1302.2 0 7975 198 15171 1302.2 0 15171 199 22810 1302.2 0 22810 200 11980 1302.2 0 11980 201 7849 1302.2 0 7849 202 7919 1302.2 0 7919 203 8418 1302.2 0 8418 204 9634 1302.2 0 9634 205 10900 1302.2 0 10900 206 12598 1302.2 0 12598 207 9776 1302.2 0 9776 208 8773 1302.2 0 8773 209 11265 1302.2 0 11265 210 8490 1302.2 0 8490 211 13354 1302.2 0 13354 212 8341 1302.2 0 8341 213 12429 1302.2 0 12429 214 9803 1302.2 0 9803 215 10849 1302.2 0 10849 216 9561 1302.2 0 9561 217 10016 1302.2 0 10016 218 13403 1302.2 0 13403 219 15104 1302.2 0 15104 220 11989 1302.2 0 11989 221 7805 1302.2 0 7805 222 8564 1302.2 0 8564 223 9197 1302.2 0 9197 224 11051 1302.2 0 11051 225 13551 1302.2 0 13551 226 11883 1302.2 0 11883 227 14946 1302.2 0 14946 228 11882 1302.2 0 11882 229 12775 1302.2 0 12775 230 14249 1302.2 0 14249 231 12241 1302.2 0 12241 232 8109 1302.2 0 8109 233 8152 1302.2 0 8152 234 12451 1302.2 0 12451 235 13562 1302.2 0 13562 236 15383 1302.2 0 15383 237 13117 1302.2 0 13117 238 12820 1302.2 0 12820 239 13320 1302.2 0 13320 240 10645 1302.2 0 10645 241 7564 1302.2 0 7564 242 8454 1302.2 0 8454 243 7731 1302.2 0 7731 244 8562 1302.2 0 8562 245 10391 1302.2 0 10391 246 12207 1302.2 0 12207 247 16922 1302.2 0 16922 248 15871 1302.2 0 15871 249 13752 1302.2 0 13752 250 10210 1302.2 0 10210 251 7969 1302.2 0 7969 252 6943 1302.2 0 6943 253 9355 1302.2 0 9355 254 10272 1302.2 0 10272 255 13677 1302.2 0 13677 256 14559 1302.2 0 14559 257 16663 1302.2 0 16663 258 11919 1302.2 0 11919 259 12640 1302.2 0 12640 260 9782 1302.2 0 9782 261 9643 1302.2 0 9643 262 7769 1302.2 0 7769 263 6424 1302.2 0 6424 264 7326 1302.2 0 7326 265 7514 1302.2 0 7514 266 10277 1302.2 0 10277 267 11008 1302.2 0 11008 268 9108 1302.2 0 9108 269 9184 1302.2 0 9184 270 8734 1302.2 0 8734 271 9556 1302.2 0 9556 272 10174 1302.2 0 10174 273 13963 1302.2 0 13963 274 12337 1302.2 0 12337 275 10457 1302.2 0 10457 276 7382 1302.2 0 7382 277 6850 1302.2 0 6850 278 8430 1302.2 0 8430 279 11919 1302.2 0 11919 280 13497 1302.2 0 13497 281 9708 1302.2 0 9708 282 12414 1302.2 0 12414 283 8246 1302.2 0 8246 284 12112 1302.2 0 12112 285 12853 1302.2 0 12853 286 11575 1302.2 0 11575 287 9920 1302.2 0 9920 288 7431 1302.2 0 7431 289 6993 1302.2 0 6993 290 7239 1302.2 0 7239 291 9053 1302.2 0 9053 292 8486 1302.2 0 8486 293 10060 1302.2 0 10060 294 9916 1302.2 0 9916 295 9389 1302.2 0 9389 296 7850 1302.2 0 7850 297 6205 1302.2 0 6205 298 4812 1302.2 0 4812 299 3736 1302.2 0 3736 300 6809 1302.2 0 6809 301 9843 1302.2 0 9843 Finished in 69.61 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 1,938,770 Reads with adapters: 15,313 (0.8%) Reads that were too short: 327 (0.0%) Reads written (passing filters): 14,986 (0.8%) Total basepairs processed: 323,093,858 bp Total written (filtered): 4,071,577 bp (1.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15313 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 14 0.0 3 0 0 9 5 16 152 0.0 3 0 99 9 44 17 14338 0.0 3 13818 447 49 24 18 72 0.0 3 5 32 23 12 19 73 0.0 3 14 1 7 51 20 27 0.0 3 0 0 0 27 21 10 0.0 3 0 0 1 9 22 7 0.0 3 0 0 0 7 23 5 0.0 3 0 0 0 5 24 6 0.0 3 0 0 0 6 25 5 0.0 3 0 0 0 5 26 5 0.0 3 0 0 0 5 27 9 0.0 3 0 0 0 9 28 7 0.0 3 0 0 1 6 29 2 0.0 3 0 0 0 2 30 2 0.0 3 0 0 0 2 31 5 0.0 3 0 0 0 5 32 2 0.0 3 0 0 0 2 33 5 0.0 3 1 0 1 3 34 4 0.0 3 0 0 0 4 35 5 0.0 3 0 0 0 5 36 1 0.0 3 0 0 0 1 37 6 0.0 3 0 0 1 5 38 2 0.0 3 0 0 1 1 39 6 0.0 3 0 0 0 6 40 5 0.0 3 0 0 0 5 41 3 0.0 3 0 0 0 3 42 2 0.0 3 0 0 0 2 43 4 0.0 3 0 0 0 4 44 3 0.0 3 0 0 0 3 45 5 0.0 3 0 0 0 5 46 8 0.0 3 0 0 0 8 47 3 0.0 3 0 0 0 3 48 3 0.0 3 0 0 0 3 49 4 0.0 3 0 0 0 4 50 4 0.0 3 0 0 0 4 51 7 0.0 3 0 0 0 7 52 2 0.0 3 0 0 0 2 53 7 0.0 3 0 0 0 7 54 8 0.0 3 0 0 0 8 55 5 0.0 3 0 0 0 5 56 1 0.0 3 0 0 0 1 57 4 0.0 3 0 0 0 4 58 8 0.0 3 0 0 0 8 59 5 0.0 3 0 0 0 5 60 5 0.0 3 0 0 1 4 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 63 6 0.0 3 0 0 0 6 64 4 0.0 3 0 0 0 4 65 6 0.0 3 0 0 0 6 66 7 0.0 3 0 0 0 7 67 15 0.0 3 0 0 0 15 68 7 0.0 3 0 0 0 7 69 10 0.0 3 0 0 0 10 70 12 0.0 3 0 0 1 11 71 5 0.0 3 0 0 0 5 72 8 0.0 3 0 0 0 8 73 6 0.0 3 0 0 0 6 74 2 0.0 3 0 0 0 2 75 4 0.0 3 0 0 0 4 76 2 0.0 3 0 0 0 2 77 4 0.0 3 0 0 0 4 78 3 0.0 3 0 0 0 3 79 5 0.0 3 0 0 0 5 80 2 0.0 3 0 0 0 2 81 4 0.0 3 0 0 0 4 82 8 0.0 3 0 0 1 7 83 2 0.0 3 0 0 0 2 84 5 0.0 3 0 0 0 5 85 2 0.0 3 0 0 0 2 86 7 0.0 3 0 0 0 7 87 5 0.0 3 0 0 0 5 88 6 0.0 3 0 0 0 6 89 5 0.0 3 0 0 0 5 90 1 0.0 3 0 0 0 1 91 3 0.0 3 0 0 0 3 92 6 0.0 3 0 0 0 6 93 8 0.0 3 0 0 0 8 94 9 0.0 3 0 0 0 9 95 3 0.0 3 0 0 0 3 96 4 0.0 3 0 0 0 4 97 5 0.0 3 0 0 0 5 98 3 0.0 3 0 0 0 3 99 6 0.0 3 0 0 0 6 101 2 0.0 3 0 0 0 2 102 3 0.0 3 0 0 0 3 103 9 0.0 3 0 0 0 9 104 5 0.0 3 0 0 0 5 105 2 0.0 3 0 0 0 2 106 3 0.0 3 0 0 0 3 107 2 0.0 3 0 0 1 1 108 2 0.0 3 0 0 0 2 109 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 111 6 0.0 3 0 0 0 6 112 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 114 6 0.0 3 0 0 0 6 115 3 0.0 3 0 0 0 3 116 1 0.0 3 0 0 0 1 117 4 0.0 3 0 0 0 4 118 3 0.0 3 0 0 0 3 119 8 0.0 3 0 0 0 8 120 4 0.0 3 0 0 0 4 121 3 0.0 3 0 0 0 3 122 11 0.0 3 0 0 2 9 123 4 0.0 3 0 0 0 4 125 2 0.0 3 0 0 0 2 126 2 0.0 3 0 0 0 2 127 4 0.0 3 0 0 0 4 129 3 0.0 3 0 0 0 3 130 2 0.0 3 0 0 0 2 131 2 0.0 3 0 0 0 2 132 3 0.0 3 0 0 0 3 133 4 0.0 3 0 0 0 4 134 3 0.0 3 0 0 0 3 135 1 0.0 3 0 0 0 1 136 3 0.0 3 0 0 0 3 137 3 0.0 3 0 0 0 3 138 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 8 0.0 3 0 0 0 8 142 3 0.0 3 0 0 0 3 143 1 0.0 3 0 0 1 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 4 0.0 3 0 0 1 3 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 5 0.0 3 0 0 0 5 153 7 0.0 3 0 0 0 7 154 2 0.0 3 0 0 0 2 155 3 0.0 3 0 0 0 3 157 4 0.0 3 0 0 0 4 158 2 0.0 3 0 0 0 2 159 4 0.0 3 0 0 0 4 160 2 0.0 3 0 0 0 2 161 3 0.0 3 0 0 0 3 162 4 0.0 3 0 0 0 4 163 2 0.0 3 0 0 1 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 5 0.0 3 0 0 0 5 170 3 0.0 3 0 0 0 3 171 4 0.0 3 0 0 0 4 172 4 0.0 3 0 0 0 4 173 3 0.0 3 0 0 0 3 174 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 176 3 0.0 3 0 0 0 3 178 1 0.0 3 0 0 0 1 179 3 0.0 3 0 0 0 3 183 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 185 3 0.0 3 0 0 0 3 186 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 190 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 197 3 0.0 3 0 0 0 3 199 2 0.0 3 0 0 0 2 200 2 0.0 3 0 0 0 2 202 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 212 2 0.0 3 0 0 0 2 215 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.69 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 14,986 Reads with adapters: 1,798 (12.0%) Reads written (passing filters): 14,986 (100.0%) Total basepairs processed: 4,071,577 bp Total written (filtered): 4,066,089 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1798 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.3% C: 11.7% G: 46.7% T: 12.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1707 234.2 0 1707 4 89 58.5 0 89 5 1 14.6 0 1 6 1 3.7 0 1