This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 168.83 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 124,213 (2.3%) Reads that were too short: 17,895 (0.3%) Reads written (passing filters): 106,318 (2.0%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 28,184,256 bp (1.8%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 124213 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1590 1302.2 0 1590 7 69822 1302.2 0 69822 8 332 1302.2 0 332 9 7038 1302.2 0 7038 10 159 1302.2 0 159 11 10 1302.2 0 10 12 18 1302.2 0 18 13 10 1302.2 0 10 14 12 1302.2 0 12 15 8 1302.2 0 8 16 47 1302.2 0 47 17 1877 1302.2 0 1877 18 705 1302.2 0 705 19 19 1302.2 0 19 20 10 1302.2 0 10 21 39 1302.2 0 39 22 618 1302.2 0 618 23 347 1302.2 0 347 24 3 1302.2 0 3 25 8 1302.2 0 8 26 30 1302.2 0 30 27 25 1302.2 0 25 28 103 1302.2 0 103 29 53 1302.2 0 53 30 497 1302.2 0 497 31 264 1302.2 0 264 32 11 1302.2 0 11 33 30 1302.2 0 30 34 12 1302.2 0 12 35 93 1302.2 0 93 36 53 1302.2 0 53 37 21 1302.2 0 21 38 8 1302.2 0 8 39 13 1302.2 0 13 40 13 1302.2 0 13 41 10 1302.2 0 10 42 35 1302.2 0 35 43 6 1302.2 0 6 44 13 1302.2 0 13 45 71 1302.2 0 71 46 6 1302.2 0 6 47 5 1302.2 0 5 48 5 1302.2 0 5 49 18 1302.2 0 18 50 11 1302.2 0 11 51 29 1302.2 0 29 52 8 1302.2 0 8 53 24 1302.2 0 24 54 19 1302.2 0 19 55 16 1302.2 0 16 56 20 1302.2 0 20 57 16 1302.2 0 16 58 15 1302.2 0 15 59 12 1302.2 0 12 60 24 1302.2 0 24 61 20 1302.2 0 20 62 12 1302.2 0 12 63 4 1302.2 0 4 64 5 1302.2 0 5 65 10 1302.2 0 10 66 26 1302.2 0 26 67 32 1302.2 0 32 68 48 1302.2 0 48 69 138 1302.2 0 138 70 9 1302.2 0 9 71 40 1302.2 0 40 72 3 1302.2 0 3 73 18 1302.2 0 18 74 9 1302.2 0 9 75 41 1302.2 0 41 76 42 1302.2 0 42 77 97 1302.2 0 97 78 182 1302.2 0 182 79 216 1302.2 0 216 80 33 1302.2 0 33 81 194 1302.2 0 194 82 380 1302.2 0 380 83 97 1302.2 0 97 84 228 1302.2 0 228 85 259 1302.2 0 259 86 54 1302.2 0 54 87 53 1302.2 0 53 88 304 1302.2 0 304 89 34 1302.2 0 34 90 23 1302.2 0 23 91 252 1302.2 0 252 92 122 1302.2 0 122 93 83 1302.2 0 83 94 177 1302.2 0 177 95 50 1302.2 0 50 96 57 1302.2 0 57 97 118 1302.2 0 118 98 225 1302.2 0 225 99 255 1302.2 0 255 100 29 1302.2 0 29 101 24 1302.2 0 24 102 11 1302.2 0 11 103 14 1302.2 0 14 104 16 1302.2 0 16 105 18 1302.2 0 18 106 9 1302.2 0 9 107 11 1302.2 0 11 108 22 1302.2 0 22 109 17 1302.2 0 17 110 8 1302.2 0 8 111 32 1302.2 0 32 112 43 1302.2 0 43 113 38 1302.2 0 38 114 227 1302.2 0 227 115 216 1302.2 0 216 116 309 1302.2 0 309 117 64 1302.2 0 64 118 48 1302.2 0 48 119 35 1302.2 0 35 120 53 1302.2 0 53 121 57 1302.2 0 57 122 70 1302.2 0 70 123 56 1302.2 0 56 124 56 1302.2 0 56 125 69 1302.2 0 69 126 50 1302.2 0 50 127 50 1302.2 0 50 128 58 1302.2 0 58 129 130 1302.2 0 130 130 154 1302.2 0 154 131 89 1302.2 0 89 132 119 1302.2 0 119 133 148 1302.2 0 148 134 157 1302.2 0 157 135 173 1302.2 0 173 136 148 1302.2 0 148 137 149 1302.2 0 149 138 202 1302.2 0 202 139 133 1302.2 0 133 140 125 1302.2 0 125 141 162 1302.2 0 162 142 156 1302.2 0 156 143 803 1302.2 0 803 144 1044 1302.2 0 1044 145 3144 1302.2 0 3144 146 90 1302.2 0 90 147 118 1302.2 0 118 148 144 1302.2 0 144 149 800 1302.2 0 800 150 880 1302.2 0 880 151 292 1302.2 0 292 152 135 1302.2 0 135 153 70 1302.2 0 70 154 138 1302.2 0 138 155 126 1302.2 0 126 156 150 1302.2 0 150 157 148 1302.2 0 148 158 110 1302.2 0 110 159 121 1302.2 0 121 160 182 1302.2 0 182 161 201 1302.2 0 201 162 164 1302.2 0 164 163 160 1302.2 0 160 164 181 1302.2 0 181 165 149 1302.2 0 149 166 183 1302.2 0 183 167 155 1302.2 0 155 168 208 1302.2 0 208 169 110 1302.2 0 110 170 80 1302.2 0 80 171 96 1302.2 0 96 172 201 1302.2 0 201 173 80 1302.2 0 80 174 61 1302.2 0 61 175 53 1302.2 0 53 176 76 1302.2 0 76 177 87 1302.2 0 87 178 117 1302.2 0 117 179 103 1302.2 0 103 180 115 1302.2 0 115 181 159 1302.2 0 159 182 197 1302.2 0 197 183 276 1302.2 0 276 184 266 1302.2 0 266 185 168 1302.2 0 168 186 131 1302.2 0 131 187 57 1302.2 0 57 188 88 1302.2 0 88 189 186 1302.2 0 186 190 168 1302.2 0 168 191 325 1302.2 0 325 192 177 1302.2 0 177 193 121 1302.2 0 121 194 94 1302.2 0 94 195 67 1302.2 0 67 196 265 1302.2 0 265 197 172 1302.2 0 172 198 50 1302.2 0 50 199 29 1302.2 0 29 200 83 1302.2 0 83 201 198 1302.2 0 198 202 139 1302.2 0 139 203 105 1302.2 0 105 204 129 1302.2 0 129 205 167 1302.2 0 167 206 134 1302.2 0 134 207 103 1302.2 0 103 208 163 1302.2 0 163 209 213 1302.2 0 213 210 195 1302.2 0 195 211 314 1302.2 0 314 212 197 1302.2 0 197 213 250 1302.2 0 250 214 257 1302.2 0 257 215 305 1302.2 0 305 216 290 1302.2 0 290 217 251 1302.2 0 251 218 165 1302.2 0 165 219 106 1302.2 0 106 220 114 1302.2 0 114 221 249 1302.2 0 249 222 286 1302.2 0 286 223 151 1302.2 0 151 224 154 1302.2 0 154 225 260 1302.2 0 260 226 214 1302.2 0 214 227 192 1302.2 0 192 228 176 1302.2 0 176 229 144 1302.2 0 144 230 115 1302.2 0 115 231 81 1302.2 0 81 232 166 1302.2 0 166 233 295 1302.2 0 295 234 210 1302.2 0 210 235 121 1302.2 0 121 236 173 1302.2 0 173 237 179 1302.2 0 179 238 100 1302.2 0 100 239 87 1302.2 0 87 240 140 1302.2 0 140 241 160 1302.2 0 160 242 169 1302.2 0 169 243 159 1302.2 0 159 244 167 1302.2 0 167 245 208 1302.2 0 208 246 205 1302.2 0 205 247 114 1302.2 0 114 248 103 1302.2 0 103 249 345 1302.2 0 345 250 322 1302.2 0 322 251 265 1302.2 0 265 252 110 1302.2 0 110 253 192 1302.2 0 192 254 304 1302.2 0 304 255 418 1302.2 0 418 256 113 1302.2 0 113 257 58 1302.2 0 58 258 66 1302.2 0 66 259 87 1302.2 0 87 260 72 1302.2 0 72 261 85 1302.2 0 85 262 94 1302.2 0 94 263 92 1302.2 0 92 264 112 1302.2 0 112 265 178 1302.2 0 178 266 133 1302.2 0 133 267 102 1302.2 0 102 268 153 1302.2 0 153 269 749 1302.2 0 749 270 984 1302.2 0 984 271 224 1302.2 0 224 272 132 1302.2 0 132 273 145 1302.2 0 145 274 169 1302.2 0 169 275 439 1302.2 0 439 276 163 1302.2 0 163 277 145 1302.2 0 145 278 122 1302.2 0 122 279 104 1302.2 0 104 280 109 1302.2 0 109 281 138 1302.2 0 138 282 122 1302.2 0 122 283 122 1302.2 0 122 284 99 1302.2 0 99 285 95 1302.2 0 95 286 91 1302.2 0 91 287 79 1302.2 0 79 288 130 1302.2 0 130 289 172 1302.2 0 172 290 185 1302.2 0 185 291 183 1302.2 0 183 292 167 1302.2 0 167 293 103 1302.2 0 103 294 110 1302.2 0 110 295 131 1302.2 0 131 296 108 1302.2 0 108 297 53 1302.2 0 53 298 265 1302.2 0 265 299 207 1302.2 0 207 300 100 1302.2 0 100 301 74 1302.2 0 74 Finished in 5.59 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 106,318 Reads with adapters: 7,429 (7.0%) Reads that were too short: 104 (0.1%) Reads written (passing filters): 7,325 (6.9%) Total basepairs processed: 28,184,256 bp Total written (filtered): 1,992,706 bp (7.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7429 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 1 0.0 3 0 0 1 16 69 0.0 3 0 50 5 14 17 6935 0.0 3 6647 245 31 12 18 99 0.0 3 1 13 82 3 19 64 0.0 3 0 1 5 58 20 11 0.0 3 0 0 0 11 30 2 0.0 3 0 0 1 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 34 90 0.0 3 0 0 1 89 35 1 0.0 3 0 0 0 1 37 6 0.0 3 0 0 0 6 38 1 0.0 3 0 0 0 1 43 4 0.0 3 0 0 0 4 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 115 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 1 1 145 2 0.0 3 0 0 0 2 146 30 0.0 3 0 0 0 30 147 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 149 8 0.0 3 0 0 1 7 156 2 0.0 3 0 0 0 2 162 16 0.0 3 0 0 0 16 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 5 0.0 3 0 0 0 5 166 2 0.0 3 0 0 0 2 179 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 0 2 226 1 0.0 3 0 0 0 1 264 2 0.0 3 0 0 0 2 270 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 12 0.0 3 0 0 0 12 282 25 0.0 3 0 0 0 25 283 5 0.0 3 0 0 0 5 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.33 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 7,325 Reads with adapters: 661 (9.0%) Reads written (passing filters): 7,325 (100.0%) Total basepairs processed: 1,992,706 bp Total written (filtered): 1,990,656 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 661 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.8% C: 14.4% G: 40.7% T: 15.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 594 114.5 0 594 4 67 28.6 0 67