This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 179.88 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 1,085,626 (20.4%) Reads that were too short: 517,770 (9.7%) Reads written (passing filters): 567,856 (10.6%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 92,231,100 bp (5.7%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 1085626 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 139 1302.2 0 139 7 1986 1302.2 0 1986 8 2126 1302.2 0 2126 9 135 1302.2 0 135 10 387 1302.2 0 387 11 14146 1302.2 0 14146 12 65 1302.2 0 65 13 23 1302.2 0 23 14 20 1302.2 0 20 15 21 1302.2 0 21 16 24 1302.2 0 24 17 10 1302.2 0 10 18 15 1302.2 0 15 19 13 1302.2 0 13 20 25 1302.2 0 25 21 11 1302.2 0 11 22 28 1302.2 0 28 23 21 1302.2 0 21 24 28 1302.2 0 28 25 14 1302.2 0 14 26 19 1302.2 0 19 27 16 1302.2 0 16 28 14 1302.2 0 14 29 15 1302.2 0 15 30 18 1302.2 0 18 31 18 1302.2 0 18 32 27 1302.2 0 27 33 18 1302.2 0 18 34 27 1302.2 0 27 35 25 1302.2 0 25 36 20 1302.2 0 20 37 19 1302.2 0 19 38 33 1302.2 0 33 39 34 1302.2 0 34 40 14 1302.2 0 14 41 21 1302.2 0 21 42 23 1302.2 0 23 43 37 1302.2 0 37 44 20 1302.2 0 20 45 18 1302.2 0 18 46 14 1302.2 0 14 47 16 1302.2 0 16 48 21 1302.2 0 21 49 22 1302.2 0 22 50 15 1302.2 0 15 51 15 1302.2 0 15 52 20 1302.2 0 20 53 40 1302.2 0 40 54 63 1302.2 0 63 55 26 1302.2 0 26 56 23 1302.2 0 23 57 24 1302.2 0 24 58 21 1302.2 0 21 59 21 1302.2 0 21 60 26 1302.2 0 26 61 20 1302.2 0 20 62 34 1302.2 0 34 63 37 1302.2 0 37 64 140 1302.2 0 140 65 67 1302.2 0 67 66 38 1302.2 0 38 67 47 1302.2 0 47 68 346 1302.2 0 346 69 200 1302.2 0 200 70 340 1302.2 0 340 71 408 1302.2 0 408 72 2743 1302.2 0 2743 73 2236 1302.2 0 2236 74 3534 1302.2 0 3534 75 3900 1302.2 0 3900 76 1551 1302.2 0 1551 77 3117 1302.2 0 3117 78 4972 1302.2 0 4972 79 2815 1302.2 0 2815 80 3418 1302.2 0 3418 81 2337 1302.2 0 2337 82 1969 1302.2 0 1969 83 1348 1302.2 0 1348 84 2663 1302.2 0 2663 85 1100 1302.2 0 1100 86 1841 1302.2 0 1841 87 3505 1302.2 0 3505 88 12096 1302.2 0 12096 89 802 1302.2 0 802 90 1447 1302.2 0 1447 91 9832 1302.2 0 9832 92 2629 1302.2 0 2629 93 5473 1302.2 0 5473 94 2269 1302.2 0 2269 95 1183 1302.2 0 1183 96 579 1302.2 0 579 97 1696 1302.2 0 1696 98 2250 1302.2 0 2250 99 1713 1302.2 0 1713 100 1314 1302.2 0 1314 101 3907 1302.2 0 3907 102 1803 1302.2 0 1803 103 3759 1302.2 0 3759 104 9740 1302.2 0 9740 105 3335 1302.2 0 3335 106 1169 1302.2 0 1169 107 3007 1302.2 0 3007 108 3876 1302.2 0 3876 109 2029 1302.2 0 2029 110 2027 1302.2 0 2027 111 2528 1302.2 0 2528 112 1551 1302.2 0 1551 113 2740 1302.2 0 2740 114 1620 1302.2 0 1620 115 1932 1302.2 0 1932 116 3255 1302.2 0 3255 117 7746 1302.2 0 7746 118 2507 1302.2 0 2507 119 5010 1302.2 0 5010 120 5797 1302.2 0 5797 121 4609 1302.2 0 4609 122 4588 1302.2 0 4588 123 7827 1302.2 0 7827 124 6390 1302.2 0 6390 125 5788 1302.2 0 5788 126 5623 1302.2 0 5623 127 2095 1302.2 0 2095 128 2325 1302.2 0 2325 129 3321 1302.2 0 3321 130 5302 1302.2 0 5302 131 3766 1302.2 0 3766 132 6716 1302.2 0 6716 133 4009 1302.2 0 4009 134 4452 1302.2 0 4452 135 4182 1302.2 0 4182 136 4226 1302.2 0 4226 137 4013 1302.2 0 4013 138 3303 1302.2 0 3303 139 3773 1302.2 0 3773 140 1845 1302.2 0 1845 141 2896 1302.2 0 2896 142 4565 1302.2 0 4565 143 7365 1302.2 0 7365 144 2994 1302.2 0 2994 145 4204 1302.2 0 4204 146 3705 1302.2 0 3705 147 3595 1302.2 0 3595 148 3892 1302.2 0 3892 149 3439 1302.2 0 3439 150 5589 1302.2 0 5589 151 5186 1302.2 0 5186 152 6005 1302.2 0 6005 153 3605 1302.2 0 3605 154 4093 1302.2 0 4093 155 6295 1302.2 0 6295 156 5038 1302.2 0 5038 157 5233 1302.2 0 5233 158 5949 1302.2 0 5949 159 3971 1302.2 0 3971 160 4554 1302.2 0 4554 161 4013 1302.2 0 4013 162 4753 1302.2 0 4753 163 5579 1302.2 0 5579 164 4719 1302.2 0 4719 165 3845 1302.2 0 3845 166 3904 1302.2 0 3904 167 4655 1302.2 0 4655 168 4574 1302.2 0 4574 169 4327 1302.2 0 4327 170 7692 1302.2 0 7692 171 13093 1302.2 0 13093 172 7932 1302.2 0 7932 173 3355 1302.2 0 3355 174 3630 1302.2 0 3630 175 3348 1302.2 0 3348 176 4859 1302.2 0 4859 177 4802 1302.2 0 4802 178 8185 1302.2 0 8185 179 6487 1302.2 0 6487 180 5894 1302.2 0 5894 181 3842 1302.2 0 3842 182 4340 1302.2 0 4340 183 5066 1302.2 0 5066 184 3744 1302.2 0 3744 185 3016 1302.2 0 3016 186 2409 1302.2 0 2409 187 4670 1302.2 0 4670 188 8547 1302.2 0 8547 189 8020 1302.2 0 8020 190 6245 1302.2 0 6245 191 4816 1302.2 0 4816 192 4369 1302.2 0 4369 193 3122 1302.2 0 3122 194 3464 1302.2 0 3464 195 9648 1302.2 0 9648 196 6240 1302.2 0 6240 197 2573 1302.2 0 2573 198 2844 1302.2 0 2844 199 2956 1302.2 0 2956 200 4415 1302.2 0 4415 201 6151 1302.2 0 6151 202 4389 1302.2 0 4389 203 3359 1302.2 0 3359 204 3900 1302.2 0 3900 205 3122 1302.2 0 3122 206 1898 1302.2 0 1898 207 3980 1302.2 0 3980 208 5487 1302.2 0 5487 209 2809 1302.2 0 2809 210 2365 1302.2 0 2365 211 3733 1302.2 0 3733 212 2620 1302.2 0 2620 213 3179 1302.2 0 3179 214 4771 1302.2 0 4771 215 6426 1302.2 0 6426 216 6909 1302.2 0 6909 217 5329 1302.2 0 5329 218 4112 1302.2 0 4112 219 2254 1302.2 0 2254 220 2631 1302.2 0 2631 221 8055 1302.2 0 8055 222 7148 1302.2 0 7148 223 4832 1302.2 0 4832 224 5224 1302.2 0 5224 225 6749 1302.2 0 6749 226 8033 1302.2 0 8033 227 10723 1302.2 0 10723 228 7330 1302.2 0 7330 229 8850 1302.2 0 8850 230 5405 1302.2 0 5405 231 5624 1302.2 0 5624 232 8577 1302.2 0 8577 233 7650 1302.2 0 7650 234 3057 1302.2 0 3057 235 2473 1302.2 0 2473 236 3854 1302.2 0 3854 237 3654 1302.2 0 3654 238 3732 1302.2 0 3732 239 4251 1302.2 0 4251 240 4215 1302.2 0 4215 241 5090 1302.2 0 5090 242 7123 1302.2 0 7123 243 4946 1302.2 0 4946 244 4238 1302.2 0 4238 245 3143 1302.2 0 3143 246 2203 1302.2 0 2203 247 2700 1302.2 0 2700 248 5209 1302.2 0 5209 249 8251 1302.2 0 8251 250 10283 1302.2 0 10283 251 9241 1302.2 0 9241 252 9835 1302.2 0 9835 253 10115 1302.2 0 10115 254 5572 1302.2 0 5572 255 4216 1302.2 0 4216 256 2589 1302.2 0 2589 257 3551 1302.2 0 3551 258 3111 1302.2 0 3111 259 3988 1302.2 0 3988 260 3889 1302.2 0 3889 261 5892 1302.2 0 5892 262 6522 1302.2 0 6522 263 9281 1302.2 0 9281 264 10655 1302.2 0 10655 265 8054 1302.2 0 8054 266 4809 1302.2 0 4809 267 2646 1302.2 0 2646 268 3285 1302.2 0 3285 269 3941 1302.2 0 3941 270 3984 1302.2 0 3984 271 5959 1302.2 0 5959 272 5457 1302.2 0 5457 273 5320 1302.2 0 5320 274 6844 1302.2 0 6844 275 7314 1302.2 0 7314 276 8747 1302.2 0 8747 277 7725 1302.2 0 7725 278 5111 1302.2 0 5111 279 3666 1302.2 0 3666 280 5161 1302.2 0 5161 281 7418 1302.2 0 7418 282 7520 1302.2 0 7520 283 5984 1302.2 0 5984 284 7076 1302.2 0 7076 285 3950 1302.2 0 3950 286 3034 1302.2 0 3034 287 3565 1302.2 0 3565 288 2814 1302.2 0 2814 289 3959 1302.2 0 3959 290 4538 1302.2 0 4538 291 6580 1302.2 0 6580 292 9898 1302.2 0 9898 293 10759 1302.2 0 10759 294 2208 1302.2 0 2208 295 2394 1302.2 0 2394 296 3000 1302.2 0 3000 297 2942 1302.2 0 2942 298 1962 1302.2 0 1962 299 2130 1302.2 0 2130 300 2163 1302.2 0 2163 301 1501 1302.2 0 1501 Finished in 20.94 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 567,856 Reads with adapters: 1,957 (0.3%) Reads that were too short: 144 (0.0%) Reads written (passing filters): 1,813 (0.3%) Total basepairs processed: 92,231,100 bp Total written (filtered): 477,294 bp (0.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1957 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 16 0.0 3 0 11 0 5 17 1628 0.0 3 1563 51 9 5 18 4 0.0 3 0 2 2 19 5 0.0 3 0 0 3 2 20 3 0.0 3 0 0 0 3 21 2 0.0 3 0 0 0 2 22 4 0.0 3 0 0 0 4 23 4 0.0 3 0 0 0 4 24 4 0.0 3 0 0 0 4 25 3 0.0 3 0 0 0 3 26 2 0.0 3 0 0 0 2 27 1 0.0 3 0 0 0 1 28 2 0.0 3 0 0 0 2 30 4 0.0 3 0 0 0 4 31 4 0.0 3 1 0 1 2 32 2 0.0 3 0 0 0 2 33 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 41 3 0.0 3 0 0 0 3 42 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 45 4 0.0 3 0 0 0 4 47 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 50 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 1 54 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 0 3 56 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 59 4 0.0 3 0 0 0 4 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 3 0.0 3 0 0 0 3 67 6 0.0 3 0 0 0 6 68 2 0.0 3 0 0 0 2 70 7 0.0 3 0 0 0 7 71 1 0.0 3 0 0 0 1 72 3 0.0 3 0 0 0 3 73 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 85 5 0.0 3 0 0 0 5 86 3 0.0 3 0 0 0 3 87 3 0.0 3 0 0 0 3 88 7 0.0 3 0 0 1 6 89 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 91 4 0.0 3 0 0 0 4 92 3 0.0 3 0 0 0 3 93 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 100 3 0.0 3 0 0 0 3 102 2 0.0 3 0 0 0 2 103 3 0.0 3 0 0 0 3 105 3 0.0 3 0 0 0 3 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 113 6 0.0 3 0 0 0 6 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 1 1 138 1 0.0 3 0 0 0 1 140 3 0.0 3 0 0 0 3 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 157 3 0.0 3 0 0 0 3 158 2 0.0 3 0 0 0 2 159 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 1 162 15 0.0 3 0 0 0 15 163 1 0.0 3 0 0 0 1 164 5 0.0 3 0 0 0 5 165 2 0.0 3 0 0 0 2 167 2 0.0 3 0 0 0 2 169 2 0.0 3 0 0 0 2 172 3 0.0 3 0 0 0 3 173 1 0.0 3 0 0 0 1 174 3 0.0 3 0 0 0 3 177 3 0.0 3 0 0 0 3 180 3 0.0 3 0 0 0 3 181 2 0.0 3 0 0 0 2 182 11 0.0 3 0 0 0 11 183 7 0.0 3 0 0 0 7 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 198 2 0.0 3 0 0 0 2 201 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 4 0.0 3 0 0 0 4 218 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 274 5 0.0 3 0 0 0 5 275 2 0.0 3 0 0 0 2 276 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 4 0.0 3 0 0 0 4 284 6 0.0 3 0 0 0 6 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 1,813 Reads with adapters: 196 (10.8%) Reads written (passing filters): 1,813 (100.0%) Total basepairs processed: 477,294 bp Total written (filtered): 476,700 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 196 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 24.0% C: 52.0% G: 9.2% T: 14.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 190 28.3 0 190 4 6 7.1 0 6