This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 176.89 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 660,537 (12.4%) Reads that were too short: 312,872 (5.9%) Reads written (passing filters): 347,665 (6.5%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 54,673,170 bp (3.4%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 660537 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 22 1302.2 0 22 7 82 1302.2 0 82 8 1577 1302.2 0 1577 9 4 1302.2 0 4 10 7 1302.2 0 7 11 11 1302.2 0 11 12 4 1302.2 0 4 13 5 1302.2 0 5 14 3 1302.2 0 3 15 6 1302.2 0 6 16 14 1302.2 0 14 17 5 1302.2 0 5 18 10 1302.2 0 10 19 9 1302.2 0 9 20 4 1302.2 0 4 21 10 1302.2 0 10 22 13 1302.2 0 13 23 12 1302.2 0 12 24 19 1302.2 0 19 25 32 1302.2 0 32 26 643 1302.2 0 643 27 432 1302.2 0 432 28 540 1302.2 0 540 29 4 1302.2 0 4 30 7 1302.2 0 7 31 4 1302.2 0 4 32 12 1302.2 0 12 33 6 1302.2 0 6 34 5 1302.2 0 5 35 3 1302.2 0 3 36 8 1302.2 0 8 37 8 1302.2 0 8 38 6 1302.2 0 6 39 7 1302.2 0 7 40 39 1302.2 0 39 41 35 1302.2 0 35 42 38 1302.2 0 38 43 12 1302.2 0 12 44 10 1302.2 0 10 45 6 1302.2 0 6 46 8 1302.2 0 8 47 7 1302.2 0 7 48 14 1302.2 0 14 49 17 1302.2 0 17 50 5 1302.2 0 5 51 7 1302.2 0 7 52 4 1302.2 0 4 53 4 1302.2 0 4 54 14 1302.2 0 14 55 4 1302.2 0 4 56 5 1302.2 0 5 57 1 1302.2 0 1 58 1 1302.2 0 1 59 5 1302.2 0 5 60 3 1302.2 0 3 61 24 1302.2 0 24 62 9 1302.2 0 9 63 10 1302.2 0 10 64 15 1302.2 0 15 65 12 1302.2 0 12 66 16 1302.2 0 16 67 11 1302.2 0 11 68 17 1302.2 0 17 69 42 1302.2 0 42 70 11 1302.2 0 11 71 193 1302.2 0 193 72 6 1302.2 0 6 73 4 1302.2 0 4 74 6 1302.2 0 6 75 6 1302.2 0 6 76 7 1302.2 0 7 77 12 1302.2 0 12 78 46 1302.2 0 46 79 205 1302.2 0 205 80 413 1302.2 0 413 81 857 1302.2 0 857 82 970 1302.2 0 970 83 902 1302.2 0 902 84 846 1302.2 0 846 85 1689 1302.2 0 1689 86 869 1302.2 0 869 87 268 1302.2 0 268 88 611 1302.2 0 611 89 1460 1302.2 0 1460 90 216 1302.2 0 216 91 1060 1302.2 0 1060 92 582 1302.2 0 582 93 571 1302.2 0 571 94 547 1302.2 0 547 95 3742 1302.2 0 3742 96 5570 1302.2 0 5570 97 2600 1302.2 0 2600 98 1483 1302.2 0 1483 99 1722 1302.2 0 1722 100 968 1302.2 0 968 101 1598 1302.2 0 1598 102 3005 1302.2 0 3005 103 1564 1302.2 0 1564 104 711 1302.2 0 711 105 3568 1302.2 0 3568 106 3257 1302.2 0 3257 107 1800 1302.2 0 1800 108 10288 1302.2 0 10288 109 6400 1302.2 0 6400 110 3782 1302.2 0 3782 111 2419 1302.2 0 2419 112 5423 1302.2 0 5423 113 3507 1302.2 0 3507 114 10343 1302.2 0 10343 115 6693 1302.2 0 6693 116 2051 1302.2 0 2051 117 1684 1302.2 0 1684 118 1006 1302.2 0 1006 119 675 1302.2 0 675 120 862 1302.2 0 862 121 1658 1302.2 0 1658 122 3824 1302.2 0 3824 123 2770 1302.2 0 2770 124 3835 1302.2 0 3835 125 1310 1302.2 0 1310 126 2440 1302.2 0 2440 127 2350 1302.2 0 2350 128 1727 1302.2 0 1727 129 4179 1302.2 0 4179 130 2762 1302.2 0 2762 131 2975 1302.2 0 2975 132 2949 1302.2 0 2949 133 2318 1302.2 0 2318 134 2039 1302.2 0 2039 135 2407 1302.2 0 2407 136 3947 1302.2 0 3947 137 5301 1302.2 0 5301 138 3563 1302.2 0 3563 139 2897 1302.2 0 2897 140 2510 1302.2 0 2510 141 5453 1302.2 0 5453 142 3612 1302.2 0 3612 143 2467 1302.2 0 2467 144 1436 1302.2 0 1436 145 1519 1302.2 0 1519 146 2573 1302.2 0 2573 147 2947 1302.2 0 2947 148 9873 1302.2 0 9873 149 3730 1302.2 0 3730 150 4645 1302.2 0 4645 151 1779 1302.2 0 1779 152 1786 1302.2 0 1786 153 3119 1302.2 0 3119 154 1894 1302.2 0 1894 155 3527 1302.2 0 3527 156 3432 1302.2 0 3432 157 3355 1302.2 0 3355 158 2357 1302.2 0 2357 159 2097 1302.2 0 2097 160 2289 1302.2 0 2289 161 2693 1302.2 0 2693 162 2157 1302.2 0 2157 163 5419 1302.2 0 5419 164 3058 1302.2 0 3058 165 4546 1302.2 0 4546 166 3167 1302.2 0 3167 167 3049 1302.2 0 3049 168 3007 1302.2 0 3007 169 3329 1302.2 0 3329 170 2488 1302.2 0 2488 171 1213 1302.2 0 1213 172 2304 1302.2 0 2304 173 1209 1302.2 0 1209 174 2719 1302.2 0 2719 175 3595 1302.2 0 3595 176 5002 1302.2 0 5002 177 2152 1302.2 0 2152 178 1483 1302.2 0 1483 179 1291 1302.2 0 1291 180 1255 1302.2 0 1255 181 2648 1302.2 0 2648 182 2999 1302.2 0 2999 183 3001 1302.2 0 3001 184 2432 1302.2 0 2432 185 3075 1302.2 0 3075 186 4994 1302.2 0 4994 187 4666 1302.2 0 4666 188 2832 1302.2 0 2832 189 1372 1302.2 0 1372 190 1630 1302.2 0 1630 191 1569 1302.2 0 1569 192 3650 1302.2 0 3650 193 4730 1302.2 0 4730 194 7104 1302.2 0 7104 195 2124 1302.2 0 2124 196 2729 1302.2 0 2729 197 1309 1302.2 0 1309 198 1698 1302.2 0 1698 199 3807 1302.2 0 3807 200 4808 1302.2 0 4808 201 4694 1302.2 0 4694 202 4675 1302.2 0 4675 203 1545 1302.2 0 1545 204 1766 1302.2 0 1766 205 3402 1302.2 0 3402 206 3967 1302.2 0 3967 207 2266 1302.2 0 2266 208 4686 1302.2 0 4686 209 4593 1302.2 0 4593 210 3721 1302.2 0 3721 211 3290 1302.2 0 3290 212 1871 1302.2 0 1871 213 2452 1302.2 0 2452 214 2141 1302.2 0 2141 215 1906 1302.2 0 1906 216 2359 1302.2 0 2359 217 2164 1302.2 0 2164 218 2792 1302.2 0 2792 219 4586 1302.2 0 4586 220 2883 1302.2 0 2883 221 2424 1302.2 0 2424 222 3500 1302.2 0 3500 223 1737 1302.2 0 1737 224 1558 1302.2 0 1558 225 1826 1302.2 0 1826 226 2494 1302.2 0 2494 227 2868 1302.2 0 2868 228 3471 1302.2 0 3471 229 3838 1302.2 0 3838 230 5138 1302.2 0 5138 231 3507 1302.2 0 3507 232 1942 1302.2 0 1942 233 1728 1302.2 0 1728 234 1425 1302.2 0 1425 235 1907 1302.2 0 1907 236 3529 1302.2 0 3529 237 5549 1302.2 0 5549 238 5108 1302.2 0 5108 239 3663 1302.2 0 3663 240 3383 1302.2 0 3383 241 1796 1302.2 0 1796 242 1865 1302.2 0 1865 243 1845 1302.2 0 1845 244 3489 1302.2 0 3489 245 4424 1302.2 0 4424 246 6717 1302.2 0 6717 247 6431 1302.2 0 6431 248 5299 1302.2 0 5299 249 3298 1302.2 0 3298 250 1191 1302.2 0 1191 251 971 1302.2 0 971 252 654 1302.2 0 654 253 1735 1302.2 0 1735 254 2249 1302.2 0 2249 255 2483 1302.2 0 2483 256 3395 1302.2 0 3395 257 5317 1302.2 0 5317 258 6370 1302.2 0 6370 259 5103 1302.2 0 5103 260 2618 1302.2 0 2618 261 1528 1302.2 0 1528 262 1442 1302.2 0 1442 263 1926 1302.2 0 1926 264 2318 1302.2 0 2318 265 2995 1302.2 0 2995 266 2771 1302.2 0 2771 267 4838 1302.2 0 4838 268 5074 1302.2 0 5074 269 8574 1302.2 0 8574 270 3792 1302.2 0 3792 271 2572 1302.2 0 2572 272 1810 1302.2 0 1810 273 2493 1302.2 0 2493 274 2975 1302.2 0 2975 275 3071 1302.2 0 3071 276 3179 1302.2 0 3179 277 1906 1302.2 0 1906 278 1475 1302.2 0 1475 279 2367 1302.2 0 2367 280 3665 1302.2 0 3665 281 3451 1302.2 0 3451 282 4492 1302.2 0 4492 283 1938 1302.2 0 1938 284 2048 1302.2 0 2048 285 2700 1302.2 0 2700 286 3164 1302.2 0 3164 287 5048 1302.2 0 5048 288 4807 1302.2 0 4807 289 3735 1302.2 0 3735 290 2338 1302.2 0 2338 291 1402 1302.2 0 1402 292 1990 1302.2 0 1990 293 3064 1302.2 0 3064 294 3480 1302.2 0 3480 295 6165 1302.2 0 6165 296 4508 1302.2 0 4508 297 2971 1302.2 0 2971 298 2350 1302.2 0 2350 299 1945 1302.2 0 1945 300 1688 1302.2 0 1688 301 3907 1302.2 0 3907 Finished in 14.40 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 347,665 Reads with adapters: 1,666 (0.5%) Reads that were too short: 90 (0.0%) Reads written (passing filters): 1,576 (0.5%) Total basepairs processed: 54,673,170 bp Total written (filtered): 425,005 bp (0.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1666 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 9 0.0 3 0 8 0 0 1 20 1522 0.0 4 1495 19 1 0 7 21 7 0.0 4 1 5 1 22 4 0.0 4 0 0 0 1 3 26 14 0.0 4 0 0 0 0 14 29 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 39 2 0.0 4 0 0 0 0 2 42 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 49 2 0.0 4 0 0 0 0 2 51 2 0.0 4 0 0 0 0 2 52 5 0.0 4 0 0 0 1 4 54 3 0.0 4 0 0 0 0 3 55 2 0.0 4 0 0 0 0 2 56 2 0.0 4 0 0 0 0 2 57 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 65 2 0.0 4 0 0 0 0 2 66 2 0.0 4 0 0 0 0 2 67 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 71 2 0.0 4 0 0 0 0 2 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 80 2 0.0 4 0 0 0 0 2 81 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 86 2 0.0 4 0 0 0 0 2 87 1 0.0 4 0 0 0 0 1 88 3 0.0 4 0 0 0 1 2 90 1 0.0 4 0 0 0 0 1 91 3 0.0 4 0 0 0 0 3 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 1 96 2 0.0 4 0 0 0 0 2 98 3 0.0 4 0 0 0 0 3 104 1 0.0 4 0 0 0 0 1 110 2 0.0 4 0 0 0 0 2 111 2 0.0 4 0 0 0 0 2 112 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 114 2 0.0 4 0 0 0 0 2 115 1 0.0 4 0 0 0 0 1 120 2 0.0 4 0 0 0 0 2 123 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 128 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 136 2 0.0 4 0 0 0 0 2 137 1 0.0 4 0 0 0 0 1 138 2 0.0 4 0 0 0 0 2 146 1 0.0 4 0 0 0 0 1 147 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 1,576 Reads with adapters: 51 (3.2%) Reads written (passing filters): 1,576 (100.0%) Total basepairs processed: 425,005 bp Total written (filtered): 423,928 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 51 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.0% C: 2.0% G: 94.1% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 11 49 0.0 1 0 49 266 1 0.0 1 1 272 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.