This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 171.55 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 249,186 (4.7%) Reads that were too short: 105,288 (2.0%) Reads written (passing filters): 143,898 (2.7%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 30,498,593 bp (1.9%) === Adapter 1 === Sequence: TGTATA; Type: regular 5'; Length: 6; Trimmed: 249186 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1100 1302.2 0 1100 7 27458 1302.2 0 27458 8 311 1302.2 0 311 9 18914 1302.2 0 18914 10 24 1302.2 0 24 11 11 1302.2 0 11 12 4 1302.2 0 4 13 8 1302.2 0 8 14 2 1302.2 0 2 15 1 1302.2 0 1 16 1 1302.2 0 1 17 13 1302.2 0 13 18 12 1302.2 0 12 19 21 1302.2 0 21 20 19 1302.2 0 19 21 107 1302.2 0 107 22 16 1302.2 0 16 23 48 1302.2 0 48 24 9 1302.2 0 9 25 159 1302.2 0 159 26 17 1302.2 0 17 27 4 1302.2 0 4 28 10 1302.2 0 10 29 3 1302.2 0 3 30 9 1302.2 0 9 31 11 1302.2 0 11 32 148 1302.2 0 148 33 69 1302.2 0 69 34 10767 1302.2 0 10767 35 524 1302.2 0 524 36 195 1302.2 0 195 37 19 1302.2 0 19 38 33 1302.2 0 33 39 8 1302.2 0 8 40 8 1302.2 0 8 41 6 1302.2 0 6 42 9 1302.2 0 9 43 6 1302.2 0 6 44 16 1302.2 0 16 45 5 1302.2 0 5 46 10 1302.2 0 10 47 3 1302.2 0 3 48 4 1302.2 0 4 49 13 1302.2 0 13 50 6 1302.2 0 6 51 4 1302.2 0 4 52 3 1302.2 0 3 53 231 1302.2 0 231 54 4 1302.2 0 4 55 5 1302.2 0 5 56 7 1302.2 0 7 57 14 1302.2 0 14 58 12 1302.2 0 12 59 28 1302.2 0 28 60 20 1302.2 0 20 61 11 1302.2 0 11 62 9 1302.2 0 9 63 3 1302.2 0 3 64 3 1302.2 0 3 65 6 1302.2 0 6 66 5 1302.2 0 5 67 6 1302.2 0 6 68 65 1302.2 0 65 69 79 1302.2 0 79 70 15 1302.2 0 15 71 3 1302.2 0 3 72 9 1302.2 0 9 73 6 1302.2 0 6 74 124 1302.2 0 124 75 46 1302.2 0 46 76 14 1302.2 0 14 77 86 1302.2 0 86 78 246 1302.2 0 246 79 450 1302.2 0 450 80 361 1302.2 0 361 81 336 1302.2 0 336 82 551 1302.2 0 551 83 489 1302.2 0 489 84 299 1302.2 0 299 85 631 1302.2 0 631 86 522 1302.2 0 522 87 813 1302.2 0 813 88 473 1302.2 0 473 89 146 1302.2 0 146 90 326 1302.2 0 326 91 210 1302.2 0 210 92 110 1302.2 0 110 93 314 1302.2 0 314 94 446 1302.2 0 446 95 1149 1302.2 0 1149 96 1748 1302.2 0 1748 97 1564 1302.2 0 1564 98 1075 1302.2 0 1075 99 1036 1302.2 0 1036 100 710 1302.2 0 710 101 1525 1302.2 0 1525 102 460 1302.2 0 460 103 174 1302.2 0 174 104 183 1302.2 0 183 105 165 1302.2 0 165 106 84 1302.2 0 84 107 198 1302.2 0 198 108 231 1302.2 0 231 109 135 1302.2 0 135 110 103 1302.2 0 103 111 664 1302.2 0 664 112 1154 1302.2 0 1154 113 1879 1302.2 0 1879 114 982 1302.2 0 982 115 558 1302.2 0 558 116 448 1302.2 0 448 117 340 1302.2 0 340 118 268 1302.2 0 268 119 345 1302.2 0 345 120 432 1302.2 0 432 121 370 1302.2 0 370 122 263 1302.2 0 263 123 296 1302.2 0 296 124 220 1302.2 0 220 125 410 1302.2 0 410 126 419 1302.2 0 419 127 551 1302.2 0 551 128 496 1302.2 0 496 129 1218 1302.2 0 1218 130 1087 1302.2 0 1087 131 1856 1302.2 0 1856 132 782 1302.2 0 782 133 1102 1302.2 0 1102 134 906 1302.2 0 906 135 602 1302.2 0 602 136 576 1302.2 0 576 137 487 1302.2 0 487 138 637 1302.2 0 637 139 654 1302.2 0 654 140 745 1302.2 0 745 141 528 1302.2 0 528 142 744 1302.2 0 744 143 906 1302.2 0 906 144 1425 1302.2 0 1425 145 765 1302.2 0 765 146 701 1302.2 0 701 147 1142 1302.2 0 1142 148 869 1302.2 0 869 149 811 1302.2 0 811 150 611 1302.2 0 611 151 448 1302.2 0 448 152 314 1302.2 0 314 153 303 1302.2 0 303 154 344 1302.2 0 344 155 471 1302.2 0 471 156 505 1302.2 0 505 157 533 1302.2 0 533 158 750 1302.2 0 750 159 791 1302.2 0 791 160 812 1302.2 0 812 161 634 1302.2 0 634 162 618 1302.2 0 618 163 535 1302.2 0 535 164 683 1302.2 0 683 165 737 1302.2 0 737 166 719 1302.2 0 719 167 768 1302.2 0 768 168 1003 1302.2 0 1003 169 1067 1302.2 0 1067 170 557 1302.2 0 557 171 737 1302.2 0 737 172 423 1302.2 0 423 173 394 1302.2 0 394 174 320 1302.2 0 320 175 1035 1302.2 0 1035 176 727 1302.2 0 727 177 587 1302.2 0 587 178 353 1302.2 0 353 179 275 1302.2 0 275 180 369 1302.2 0 369 181 706 1302.2 0 706 182 873 1302.2 0 873 183 853 1302.2 0 853 184 808 1302.2 0 808 185 1179 1302.2 0 1179 186 1140 1302.2 0 1140 187 1087 1302.2 0 1087 188 846 1302.2 0 846 189 428 1302.2 0 428 190 470 1302.2 0 470 191 324 1302.2 0 324 192 677 1302.2 0 677 193 1697 1302.2 0 1697 194 1268 1302.2 0 1268 195 1785 1302.2 0 1785 196 703 1302.2 0 703 197 442 1302.2 0 442 198 373 1302.2 0 373 199 902 1302.2 0 902 200 1117 1302.2 0 1117 201 937 1302.2 0 937 202 298 1302.2 0 298 203 264 1302.2 0 264 204 592 1302.2 0 592 205 1265 1302.2 0 1265 206 1438 1302.2 0 1438 207 1513 1302.2 0 1513 208 975 1302.2 0 975 209 632 1302.2 0 632 210 489 1302.2 0 489 211 640 1302.2 0 640 212 987 1302.2 0 987 213 1309 1302.2 0 1309 214 1106 1302.2 0 1106 215 1094 1302.2 0 1094 216 1002 1302.2 0 1002 217 916 1302.2 0 916 218 1127 1302.2 0 1127 219 1548 1302.2 0 1548 220 1438 1302.2 0 1438 221 1127 1302.2 0 1127 222 548 1302.2 0 548 223 416 1302.2 0 416 224 760 1302.2 0 760 225 979 1302.2 0 979 226 1049 1302.2 0 1049 227 1223 1302.2 0 1223 228 1261 1302.2 0 1261 229 1775 1302.2 0 1775 230 1485 1302.2 0 1485 231 1110 1302.2 0 1110 232 919 1302.2 0 919 233 549 1302.2 0 549 234 573 1302.2 0 573 235 942 1302.2 0 942 236 1634 1302.2 0 1634 237 1503 1302.2 0 1503 238 1136 1302.2 0 1136 239 1262 1302.2 0 1262 240 1073 1302.2 0 1073 241 814 1302.2 0 814 242 519 1302.2 0 519 243 510 1302.2 0 510 244 636 1302.2 0 636 245 1054 1302.2 0 1054 246 1440 1302.2 0 1440 247 1566 1302.2 0 1566 248 1845 1302.2 0 1845 249 990 1302.2 0 990 250 616 1302.2 0 616 251 296 1302.2 0 296 252 250 1302.2 0 250 253 323 1302.2 0 323 254 715 1302.2 0 715 255 863 1302.2 0 863 256 933 1302.2 0 933 257 1202 1302.2 0 1202 258 1730 1302.2 0 1730 259 1507 1302.2 0 1507 260 949 1302.2 0 949 261 572 1302.2 0 572 262 463 1302.2 0 463 263 506 1302.2 0 506 264 376 1302.2 0 376 265 501 1302.2 0 501 266 1016 1302.2 0 1016 267 1848 1302.2 0 1848 268 2210 1302.2 0 2210 269 2509 1302.2 0 2509 270 1506 1302.2 0 1506 271 645 1302.2 0 645 272 662 1302.2 0 662 273 855 1302.2 0 855 274 1324 1302.2 0 1324 275 1457 1302.2 0 1457 276 985 1302.2 0 985 277 646 1302.2 0 646 278 475 1302.2 0 475 279 826 1302.2 0 826 280 1452 1302.2 0 1452 281 1767 1302.2 0 1767 282 1524 1302.2 0 1524 283 888 1302.2 0 888 284 921 1302.2 0 921 285 532 1302.2 0 532 286 813 1302.2 0 813 287 1050 1302.2 0 1050 288 1504 1302.2 0 1504 289 912 1302.2 0 912 290 1190 1302.2 0 1190 291 820 1302.2 0 820 292 1147 1302.2 0 1147 293 1302 1302.2 0 1302 294 2116 1302.2 0 2116 295 1872 1302.2 0 1872 296 1173 1302.2 0 1173 297 674 1302.2 0 674 298 1180 1302.2 0 1180 299 1185 1302.2 0 1185 300 1939 1302.2 0 1939 301 1230 1302.2 0 1230 Finished in 6.35 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 143,898 Reads with adapters: 19,468 (13.5%) Reads that were too short: 60 (0.0%) Reads written (passing filters): 19,408 (13.5%) Total basepairs processed: 30,498,593 bp Total written (filtered): 5,327,565 bp (17.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 19468 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 6 0.0 3 0 0 5 1 16 193 0.0 3 0 183 6 4 17 19036 0.0 3 18420 535 63 18 18 57 0.0 3 5 42 5 5 19 24 0.0 3 9 2 6 7 20 18 0.0 3 1 0 0 17 21 1 0.0 3 1 22 1 0.0 3 0 0 0 1 25 1 0.0 3 1 29 5 0.0 3 0 0 0 5 30 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 62 3 0.0 3 0 0 0 3 63 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 72 3 0.0 3 0 0 0 3 73 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 4 0.0 3 0 0 0 4 99 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 141 7 0.0 3 0 0 0 7 142 2 0.0 3 0 0 0 2 144 10 0.0 3 0 0 0 10 145 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 158 5 0.0 3 0 0 0 5 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 4 0.0 3 0 0 0 4 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 192 6 0.0 3 0 0 0 6 193 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 1 267 1 0.0 3 0 0 0 1 281 8 0.0 3 0 0 0 8 282 14 0.0 3 0 0 0 14 288 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.88 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 19,408 Reads with adapters: 834 (4.3%) Reads written (passing filters): 19,408 (100.0%) Total basepairs processed: 5,327,565 bp Total written (filtered): 5,324,948 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 834 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.8% C: 3.5% G: 27.7% T: 35.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 720 303.2 0 720 4 113 75.8 0 113 5 1 19.0 0 1