This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 169.19 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 100,063 (1.9%) Reads that were too short: 40,622 (0.8%) Reads written (passing filters): 59,441 (1.1%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 11,379,334 bp (0.7%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 100063 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 347 1302.2 0 347 7 11097 1302.2 0 11097 8 64 1302.2 0 64 9 2734 1302.2 0 2734 10 19 1302.2 0 19 11 7 1302.2 0 7 12 11 1302.2 0 11 13 17 1302.2 0 17 14 18 1302.2 0 18 15 7 1302.2 0 7 16 24 1302.2 0 24 17 35 1302.2 0 35 18 17 1302.2 0 17 19 6 1302.2 0 6 20 9 1302.2 0 9 21 7 1302.2 0 7 22 12 1302.2 0 12 23 9 1302.2 0 9 24 9 1302.2 0 9 25 14 1302.2 0 14 26 17 1302.2 0 17 27 18 1302.2 0 18 28 15 1302.2 0 15 29 16 1302.2 0 16 30 23 1302.2 0 23 31 19 1302.2 0 19 32 7 1302.2 0 7 33 17 1302.2 0 17 34 12 1302.2 0 12 35 11 1302.2 0 11 36 16 1302.2 0 16 37 15 1302.2 0 15 38 7 1302.2 0 7 39 30 1302.2 0 30 40 23 1302.2 0 23 41 31 1302.2 0 31 42 23 1302.2 0 23 43 18 1302.2 0 18 44 136 1302.2 0 136 45 48 1302.2 0 48 46 24 1302.2 0 24 47 9 1302.2 0 9 48 8 1302.2 0 8 49 13 1302.2 0 13 50 18 1302.2 0 18 51 21 1302.2 0 21 52 15 1302.2 0 15 53 107 1302.2 0 107 54 10 1302.2 0 10 55 8 1302.2 0 8 56 104 1302.2 0 104 57 23 1302.2 0 23 58 51 1302.2 0 51 59 52 1302.2 0 52 60 23 1302.2 0 23 61 14 1302.2 0 14 62 8 1302.2 0 8 63 12 1302.2 0 12 64 5 1302.2 0 5 65 5 1302.2 0 5 66 16 1302.2 0 16 67 16 1302.2 0 16 68 20 1302.2 0 20 69 11 1302.2 0 11 70 5 1302.2 0 5 71 15 1302.2 0 15 72 9 1302.2 0 9 73 43 1302.2 0 43 74 181 1302.2 0 181 75 440 1302.2 0 440 76 33 1302.2 0 33 77 23 1302.2 0 23 78 48 1302.2 0 48 79 85 1302.2 0 85 80 86 1302.2 0 86 81 278 1302.2 0 278 82 310 1302.2 0 310 83 279 1302.2 0 279 84 160 1302.2 0 160 85 119 1302.2 0 119 86 107 1302.2 0 107 87 91 1302.2 0 91 88 98 1302.2 0 98 89 30 1302.2 0 30 90 128 1302.2 0 128 91 340 1302.2 0 340 92 253 1302.2 0 253 93 405 1302.2 0 405 94 277 1302.2 0 277 95 98 1302.2 0 98 96 202 1302.2 0 202 97 536 1302.2 0 536 98 207 1302.2 0 207 99 87 1302.2 0 87 100 29 1302.2 0 29 101 48 1302.2 0 48 102 51 1302.2 0 51 103 84 1302.2 0 84 104 209 1302.2 0 209 105 57 1302.2 0 57 106 519 1302.2 0 519 107 899 1302.2 0 899 108 1447 1302.2 0 1447 109 65 1302.2 0 65 110 74 1302.2 0 74 111 63 1302.2 0 63 112 93 1302.2 0 93 113 175 1302.2 0 175 114 289 1302.2 0 289 115 393 1302.2 0 393 116 559 1302.2 0 559 117 87 1302.2 0 87 118 99 1302.2 0 99 119 377 1302.2 0 377 120 254 1302.2 0 254 121 307 1302.2 0 307 122 223 1302.2 0 223 123 196 1302.2 0 196 124 243 1302.2 0 243 125 358 1302.2 0 358 126 183 1302.2 0 183 127 223 1302.2 0 223 128 493 1302.2 0 493 129 388 1302.2 0 388 130 425 1302.2 0 425 131 130 1302.2 0 130 132 114 1302.2 0 114 133 243 1302.2 0 243 134 511 1302.2 0 511 135 444 1302.2 0 444 136 362 1302.2 0 362 137 272 1302.2 0 272 138 462 1302.2 0 462 139 379 1302.2 0 379 140 386 1302.2 0 386 141 591 1302.2 0 591 142 1055 1302.2 0 1055 143 380 1302.2 0 380 144 207 1302.2 0 207 145 186 1302.2 0 186 146 242 1302.2 0 242 147 293 1302.2 0 293 148 1746 1302.2 0 1746 149 1001 1302.2 0 1001 150 1622 1302.2 0 1622 151 151 1302.2 0 151 152 153 1302.2 0 153 153 209 1302.2 0 209 154 289 1302.2 0 289 155 1450 1302.2 0 1450 156 422 1302.2 0 422 157 505 1302.2 0 505 158 178 1302.2 0 178 159 235 1302.2 0 235 160 333 1302.2 0 333 161 416 1302.2 0 416 162 425 1302.2 0 425 163 1401 1302.2 0 1401 164 689 1302.2 0 689 165 944 1302.2 0 944 166 515 1302.2 0 515 167 456 1302.2 0 456 168 360 1302.2 0 360 169 221 1302.2 0 221 170 192 1302.2 0 192 171 310 1302.2 0 310 172 236 1302.2 0 236 173 131 1302.2 0 131 174 152 1302.2 0 152 175 305 1302.2 0 305 176 179 1302.2 0 179 177 200 1302.2 0 200 178 148 1302.2 0 148 179 133 1302.2 0 133 180 575 1302.2 0 575 181 985 1302.2 0 985 182 929 1302.2 0 929 183 567 1302.2 0 567 184 546 1302.2 0 546 185 471 1302.2 0 471 186 238 1302.2 0 238 187 199 1302.2 0 199 188 202 1302.2 0 202 189 244 1302.2 0 244 190 163 1302.2 0 163 191 307 1302.2 0 307 192 333 1302.2 0 333 193 300 1302.2 0 300 194 222 1302.2 0 222 195 305 1302.2 0 305 196 255 1302.2 0 255 197 97 1302.2 0 97 198 94 1302.2 0 94 199 166 1302.2 0 166 200 486 1302.2 0 486 201 352 1302.2 0 352 202 239 1302.2 0 239 203 231 1302.2 0 231 204 485 1302.2 0 485 205 392 1302.2 0 392 206 274 1302.2 0 274 207 499 1302.2 0 499 208 350 1302.2 0 350 209 251 1302.2 0 251 210 398 1302.2 0 398 211 283 1302.2 0 283 212 478 1302.2 0 478 213 313 1302.2 0 313 214 363 1302.2 0 363 215 519 1302.2 0 519 216 528 1302.2 0 528 217 422 1302.2 0 422 218 324 1302.2 0 324 219 253 1302.2 0 253 220 430 1302.2 0 430 221 498 1302.2 0 498 222 144 1302.2 0 144 223 112 1302.2 0 112 224 376 1302.2 0 376 225 488 1302.2 0 488 226 758 1302.2 0 758 227 720 1302.2 0 720 228 529 1302.2 0 529 229 391 1302.2 0 391 230 262 1302.2 0 262 231 350 1302.2 0 350 232 785 1302.2 0 785 233 449 1302.2 0 449 234 242 1302.2 0 242 235 341 1302.2 0 341 236 430 1302.2 0 430 237 334 1302.2 0 334 238 172 1302.2 0 172 239 131 1302.2 0 131 240 191 1302.2 0 191 241 180 1302.2 0 180 242 206 1302.2 0 206 243 171 1302.2 0 171 244 336 1302.2 0 336 245 402 1302.2 0 402 246 499 1302.2 0 499 247 378 1302.2 0 378 248 336 1302.2 0 336 249 285 1302.2 0 285 250 312 1302.2 0 312 251 154 1302.2 0 154 252 129 1302.2 0 129 253 310 1302.2 0 310 254 814 1302.2 0 814 255 1048 1302.2 0 1048 256 821 1302.2 0 821 257 592 1302.2 0 592 258 595 1302.2 0 595 259 310 1302.2 0 310 260 188 1302.2 0 188 261 108 1302.2 0 108 262 144 1302.2 0 144 263 204 1302.2 0 204 264 168 1302.2 0 168 265 326 1302.2 0 326 266 577 1302.2 0 577 267 498 1302.2 0 498 268 862 1302.2 0 862 269 1395 1302.2 0 1395 270 525 1302.2 0 525 271 442 1302.2 0 442 272 366 1302.2 0 366 273 337 1302.2 0 337 274 203 1302.2 0 203 275 334 1302.2 0 334 276 358 1302.2 0 358 277 261 1302.2 0 261 278 256 1302.2 0 256 279 332 1302.2 0 332 280 540 1302.2 0 540 281 503 1302.2 0 503 282 325 1302.2 0 325 283 292 1302.2 0 292 284 317 1302.2 0 317 285 249 1302.2 0 249 286 490 1302.2 0 490 287 386 1302.2 0 386 288 347 1302.2 0 347 289 522 1302.2 0 522 290 377 1302.2 0 377 291 289 1302.2 0 289 292 350 1302.2 0 350 293 430 1302.2 0 430 294 793 1302.2 0 793 295 1610 1302.2 0 1610 296 765 1302.2 0 765 297 633 1302.2 0 633 298 515 1302.2 0 515 299 237 1302.2 0 237 300 115 1302.2 0 115 301 40 1302.2 0 40 Finished in 2.55 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 59,441 Reads with adapters: 11,347 (19.1%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 11,329 (19.1%) Total basepairs processed: 11,379,334 bp Total written (filtered): 3,127,249 bp (27.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11347 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 1 0.0 3 0 0 1 16 87 0.0 3 0 75 9 3 17 10999 0.0 3 10664 298 28 9 18 63 0.0 3 4 31 26 2 19 43 0.0 3 3 1 13 26 20 67 0.0 3 55 0 0 12 21 1 0.0 3 0 1 30 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 43 32 0.0 3 0 0 0 32 45 1 0.0 3 0 0 0 1 46 8 0.0 3 0 0 0 8 47 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 69 8 0.0 3 0 0 0 8 72 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 145 7 0.0 3 0 0 0 7 155 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.50 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 11,329 Reads with adapters: 883 (7.8%) Reads written (passing filters): 11,329 (100.0%) Total basepairs processed: 3,127,249 bp Total written (filtered): 3,124,501 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 883 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 22.8% C: 50.7% G: 8.7% T: 17.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 785 177.0 0 785 4 97 44.3 0 97 5 1 11.1 0 1