This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 172.68 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 57,944 (1.1%) Reads that were too short: 25,369 (0.5%) Reads written (passing filters): 32,575 (0.6%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 6,641,210 bp (0.4%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 57944 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 84 1302.2 0 84 7 261 1302.2 0 261 8 218 1302.2 0 218 9 10928 1302.2 0 10928 10 23 1302.2 0 23 11 8 1302.2 0 8 12 13 1302.2 0 13 13 10 1302.2 0 10 14 7 1302.2 0 7 15 6 1302.2 0 6 16 8 1302.2 0 8 17 8 1302.2 0 8 18 5 1302.2 0 5 19 7 1302.2 0 7 20 5 1302.2 0 5 21 5 1302.2 0 5 22 8 1302.2 0 8 23 8 1302.2 0 8 24 9 1302.2 0 9 25 9 1302.2 0 9 26 12 1302.2 0 12 27 9 1302.2 0 9 28 6 1302.2 0 6 29 4 1302.2 0 4 30 5 1302.2 0 5 31 10 1302.2 0 10 32 7 1302.2 0 7 33 13 1302.2 0 13 34 6 1302.2 0 6 35 7 1302.2 0 7 36 5 1302.2 0 5 37 13 1302.2 0 13 38 140 1302.2 0 140 39 7 1302.2 0 7 40 14 1302.2 0 14 41 11 1302.2 0 11 42 12 1302.2 0 12 43 18 1302.2 0 18 44 66 1302.2 0 66 45 13 1302.2 0 13 46 15 1302.2 0 15 47 7 1302.2 0 7 48 6 1302.2 0 6 49 2 1302.2 0 2 50 5 1302.2 0 5 51 14 1302.2 0 14 52 6 1302.2 0 6 53 8 1302.2 0 8 54 14 1302.2 0 14 55 5 1302.2 0 5 56 12 1302.2 0 12 57 8 1302.2 0 8 58 14 1302.2 0 14 59 2 1302.2 0 2 60 4 1302.2 0 4 61 8 1302.2 0 8 62 6 1302.2 0 6 63 2 1302.2 0 2 64 6 1302.2 0 6 65 10 1302.2 0 10 66 7 1302.2 0 7 67 14 1302.2 0 14 68 11 1302.2 0 11 69 43 1302.2 0 43 70 18 1302.2 0 18 71 247 1302.2 0 247 72 8 1302.2 0 8 73 5 1302.2 0 5 74 74 1302.2 0 74 75 21 1302.2 0 21 76 59 1302.2 0 59 77 8 1302.2 0 8 78 34 1302.2 0 34 79 168 1302.2 0 168 80 119 1302.2 0 119 81 210 1302.2 0 210 82 1553 1302.2 0 1553 83 182 1302.2 0 182 84 387 1302.2 0 387 85 40 1302.2 0 40 86 25 1302.2 0 25 87 54 1302.2 0 54 88 49 1302.2 0 49 89 15 1302.2 0 15 90 5 1302.2 0 5 91 59 1302.2 0 59 92 33 1302.2 0 33 93 52 1302.2 0 52 94 42 1302.2 0 42 95 42 1302.2 0 42 96 29 1302.2 0 29 97 101 1302.2 0 101 98 55 1302.2 0 55 99 30 1302.2 0 30 100 27 1302.2 0 27 101 92 1302.2 0 92 102 31 1302.2 0 31 103 38 1302.2 0 38 104 47 1302.2 0 47 105 21 1302.2 0 21 106 12 1302.2 0 12 107 32 1302.2 0 32 108 33 1302.2 0 33 109 14 1302.2 0 14 110 23 1302.2 0 23 111 42 1302.2 0 42 112 25 1302.2 0 25 113 40 1302.2 0 40 114 35 1302.2 0 35 115 43 1302.2 0 43 116 40 1302.2 0 40 117 178 1302.2 0 178 118 103 1302.2 0 103 119 302 1302.2 0 302 120 135 1302.2 0 135 121 146 1302.2 0 146 122 134 1302.2 0 134 123 202 1302.2 0 202 124 87 1302.2 0 87 125 169 1302.2 0 169 126 86 1302.2 0 86 127 56 1302.2 0 56 128 69 1302.2 0 69 129 71 1302.2 0 71 130 88 1302.2 0 88 131 96 1302.2 0 96 132 237 1302.2 0 237 133 169 1302.2 0 169 134 163 1302.2 0 163 135 125 1302.2 0 125 136 118 1302.2 0 118 137 173 1302.2 0 173 138 171 1302.2 0 171 139 138 1302.2 0 138 140 87 1302.2 0 87 141 53 1302.2 0 53 142 93 1302.2 0 93 143 171 1302.2 0 171 144 172 1302.2 0 172 145 129 1302.2 0 129 146 152 1302.2 0 152 147 76 1302.2 0 76 148 138 1302.2 0 138 149 47 1302.2 0 47 150 50 1302.2 0 50 151 60 1302.2 0 60 152 100 1302.2 0 100 153 172 1302.2 0 172 154 200 1302.2 0 200 155 153 1302.2 0 153 156 116 1302.2 0 116 157 133 1302.2 0 133 158 159 1302.2 0 159 159 188 1302.2 0 188 160 85 1302.2 0 85 161 95 1302.2 0 95 162 107 1302.2 0 107 163 219 1302.2 0 219 164 162 1302.2 0 162 165 180 1302.2 0 180 166 158 1302.2 0 158 167 603 1302.2 0 603 168 403 1302.2 0 403 169 858 1302.2 0 858 170 153 1302.2 0 153 171 520 1302.2 0 520 172 453 1302.2 0 453 173 250 1302.2 0 250 174 188 1302.2 0 188 175 57 1302.2 0 57 176 58 1302.2 0 58 177 106 1302.2 0 106 178 142 1302.2 0 142 179 192 1302.2 0 192 180 204 1302.2 0 204 181 173 1302.2 0 173 182 151 1302.2 0 151 183 194 1302.2 0 194 184 204 1302.2 0 204 185 129 1302.2 0 129 186 89 1302.2 0 89 187 79 1302.2 0 79 188 161 1302.2 0 161 189 493 1302.2 0 493 190 437 1302.2 0 437 191 169 1302.2 0 169 192 119 1302.2 0 119 193 260 1302.2 0 260 194 85 1302.2 0 85 195 93 1302.2 0 93 196 223 1302.2 0 223 197 128 1302.2 0 128 198 82 1302.2 0 82 199 104 1302.2 0 104 200 284 1302.2 0 284 201 1504 1302.2 0 1504 202 925 1302.2 0 925 203 253 1302.2 0 253 204 174 1302.2 0 174 205 120 1302.2 0 120 206 140 1302.2 0 140 207 109 1302.2 0 109 208 149 1302.2 0 149 209 120 1302.2 0 120 210 156 1302.2 0 156 211 170 1302.2 0 170 212 196 1302.2 0 196 213 146 1302.2 0 146 214 153 1302.2 0 153 215 339 1302.2 0 339 216 277 1302.2 0 277 217 264 1302.2 0 264 218 142 1302.2 0 142 219 115 1302.2 0 115 220 122 1302.2 0 122 221 128 1302.2 0 128 222 237 1302.2 0 237 223 258 1302.2 0 258 224 485 1302.2 0 485 225 385 1302.2 0 385 226 351 1302.2 0 351 227 832 1302.2 0 832 228 1633 1302.2 0 1633 229 891 1302.2 0 891 230 1326 1302.2 0 1326 231 101 1302.2 0 101 232 114 1302.2 0 114 233 128 1302.2 0 128 234 119 1302.2 0 119 235 131 1302.2 0 131 236 104 1302.2 0 104 237 94 1302.2 0 94 238 107 1302.2 0 107 239 100 1302.2 0 100 240 115 1302.2 0 115 241 90 1302.2 0 90 242 141 1302.2 0 141 243 282 1302.2 0 282 244 302 1302.2 0 302 245 251 1302.2 0 251 246 95 1302.2 0 95 247 91 1302.2 0 91 248 74 1302.2 0 74 249 141 1302.2 0 141 250 270 1302.2 0 270 251 302 1302.2 0 302 252 213 1302.2 0 213 253 375 1302.2 0 375 254 392 1302.2 0 392 255 273 1302.2 0 273 256 193 1302.2 0 193 257 214 1302.2 0 214 258 315 1302.2 0 315 259 188 1302.2 0 188 260 160 1302.2 0 160 261 517 1302.2 0 517 262 705 1302.2 0 705 263 633 1302.2 0 633 264 413 1302.2 0 413 265 370 1302.2 0 370 266 125 1302.2 0 125 267 87 1302.2 0 87 268 192 1302.2 0 192 269 715 1302.2 0 715 270 630 1302.2 0 630 271 318 1302.2 0 318 272 162 1302.2 0 162 273 125 1302.2 0 125 274 118 1302.2 0 118 275 143 1302.2 0 143 276 127 1302.2 0 127 277 162 1302.2 0 162 278 178 1302.2 0 178 279 123 1302.2 0 123 280 97 1302.2 0 97 281 117 1302.2 0 117 282 290 1302.2 0 290 283 251 1302.2 0 251 284 222 1302.2 0 222 285 138 1302.2 0 138 286 130 1302.2 0 130 287 61 1302.2 0 61 288 56 1302.2 0 56 289 77 1302.2 0 77 290 134 1302.2 0 134 291 170 1302.2 0 170 292 173 1302.2 0 173 293 135 1302.2 0 135 294 68 1302.2 0 68 295 38 1302.2 0 38 296 41 1302.2 0 41 297 73 1302.2 0 73 298 189 1302.2 0 189 299 299 1302.2 0 299 300 603 1302.2 0 603 301 93 1302.2 0 93 Finished in 1.57 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 32,575 Reads with adapters: 11,163 (34.3%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 11,158 (34.3%) Total basepairs processed: 6,641,210 bp Total written (filtered): 3,035,178 bp (45.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11163 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 17 1 0.0 3 0 0 0 0 1 18 5 0.0 3 0 0 4 1 19 100 0.0 3 0 97 1 1 1 20 11004 0.0 4 10823 170 3 5 3 21 29 0.0 4 5 22 1 1 22 10 0.0 4 2 0 2 1 5 23 3 0.0 4 0 0 0 2 1 25 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.48 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 11,158 Reads with adapters: 1,017 (9.1%) Reads written (passing filters): 11,158 (100.0%) Total basepairs processed: 3,035,178 bp Total written (filtered): 2,808,034 bp (92.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1017 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 84.3% C: 2.3% G: 12.9% T: 0.6% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 5 1 10.9 0 1 8 1 0.2 0 1 9 31 0.0 0 0 31 10 38 0.0 1 2 36 11 65 0.0 1 0 65 35 2 0.0 1 1 1 156 3 0.0 1 3 256 5 0.0 1 5 257 828 0.0 1 773 55 258 27 0.0 1 25 2 259 6 0.0 1 6 260 5 0.0 1 5 263 4 0.0 1 2 2 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.