This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 169.40 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 90,128 (1.7%) Reads that were too short: 35,402 (0.7%) Reads written (passing filters): 54,726 (1.0%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 12,996,997 bp (0.8%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 90128 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 619 1302.2 0 619 7 14592 1302.2 0 14592 8 306 1302.2 0 306 9 9535 1302.2 0 9535 10 21 1302.2 0 21 11 10 1302.2 0 10 12 21 1302.2 0 21 13 17 1302.2 0 17 14 26 1302.2 0 26 15 22 1302.2 0 22 16 5066 1302.2 0 5066 17 191 1302.2 0 191 18 29 1302.2 0 29 19 19 1302.2 0 19 20 15 1302.2 0 15 21 15 1302.2 0 15 22 26 1302.2 0 26 23 14 1302.2 0 14 24 17 1302.2 0 17 25 31 1302.2 0 31 26 19 1302.2 0 19 27 18 1302.2 0 18 28 15 1302.2 0 15 29 22 1302.2 0 22 30 29 1302.2 0 29 31 23 1302.2 0 23 32 19 1302.2 0 19 33 22 1302.2 0 22 34 8 1302.2 0 8 35 17 1302.2 0 17 36 20 1302.2 0 20 37 13 1302.2 0 13 38 21 1302.2 0 21 39 16 1302.2 0 16 40 13 1302.2 0 13 41 23 1302.2 0 23 42 14 1302.2 0 14 43 24 1302.2 0 24 44 33 1302.2 0 33 45 13 1302.2 0 13 46 27 1302.2 0 27 47 15 1302.2 0 15 48 19 1302.2 0 19 49 34 1302.2 0 34 50 2637 1302.2 0 2637 51 141 1302.2 0 141 52 42 1302.2 0 42 53 26 1302.2 0 26 54 13 1302.2 0 13 55 17 1302.2 0 17 56 40 1302.2 0 40 57 10 1302.2 0 10 58 20 1302.2 0 20 59 20 1302.2 0 20 60 14 1302.2 0 14 61 21 1302.2 0 21 62 13 1302.2 0 13 63 12 1302.2 0 12 64 15 1302.2 0 15 65 15 1302.2 0 15 66 12 1302.2 0 12 67 31 1302.2 0 31 68 221 1302.2 0 221 69 95 1302.2 0 95 70 11 1302.2 0 11 71 42 1302.2 0 42 72 21 1302.2 0 21 73 16 1302.2 0 16 74 63 1302.2 0 63 75 37 1302.2 0 37 76 43 1302.2 0 43 77 19 1302.2 0 19 78 22 1302.2 0 22 79 17 1302.2 0 17 80 64 1302.2 0 64 81 137 1302.2 0 137 82 70 1302.2 0 70 83 275 1302.2 0 275 84 526 1302.2 0 526 85 151 1302.2 0 151 86 268 1302.2 0 268 87 435 1302.2 0 435 88 273 1302.2 0 273 89 97 1302.2 0 97 90 137 1302.2 0 137 91 144 1302.2 0 144 92 359 1302.2 0 359 93 269 1302.2 0 269 94 151 1302.2 0 151 95 70 1302.2 0 70 96 83 1302.2 0 83 97 49 1302.2 0 49 98 217 1302.2 0 217 99 44 1302.2 0 44 100 25 1302.2 0 25 101 11 1302.2 0 11 102 21 1302.2 0 21 103 20 1302.2 0 20 104 34 1302.2 0 34 105 61 1302.2 0 61 106 15 1302.2 0 15 107 13 1302.2 0 13 108 43 1302.2 0 43 109 29 1302.2 0 29 110 24 1302.2 0 24 111 37 1302.2 0 37 112 29 1302.2 0 29 113 40 1302.2 0 40 114 62 1302.2 0 62 115 46 1302.2 0 46 116 58 1302.2 0 58 117 107 1302.2 0 107 118 104 1302.2 0 104 119 162 1302.2 0 162 120 125 1302.2 0 125 121 283 1302.2 0 283 122 105 1302.2 0 105 123 81 1302.2 0 81 124 129 1302.2 0 129 125 91 1302.2 0 91 126 86 1302.2 0 86 127 69 1302.2 0 69 128 74 1302.2 0 74 129 97 1302.2 0 97 130 289 1302.2 0 289 131 62 1302.2 0 62 132 159 1302.2 0 159 133 125 1302.2 0 125 134 305 1302.2 0 305 135 129 1302.2 0 129 136 84 1302.2 0 84 137 86 1302.2 0 86 138 137 1302.2 0 137 139 132 1302.2 0 132 140 107 1302.2 0 107 141 85 1302.2 0 85 142 77 1302.2 0 77 143 142 1302.2 0 142 144 102 1302.2 0 102 145 126 1302.2 0 126 146 105 1302.2 0 105 147 125 1302.2 0 125 148 123 1302.2 0 123 149 104 1302.2 0 104 150 74 1302.2 0 74 151 89 1302.2 0 89 152 144 1302.2 0 144 153 233 1302.2 0 233 154 291 1302.2 0 291 155 220 1302.2 0 220 156 203 1302.2 0 203 157 119 1302.2 0 119 158 192 1302.2 0 192 159 147 1302.2 0 147 160 286 1302.2 0 286 161 182 1302.2 0 182 162 175 1302.2 0 175 163 277 1302.2 0 277 164 318 1302.2 0 318 165 337 1302.2 0 337 166 214 1302.2 0 214 167 208 1302.2 0 208 168 188 1302.2 0 188 169 120 1302.2 0 120 170 75 1302.2 0 75 171 124 1302.2 0 124 172 348 1302.2 0 348 173 138 1302.2 0 138 174 135 1302.2 0 135 175 139 1302.2 0 139 176 113 1302.2 0 113 177 114 1302.2 0 114 178 207 1302.2 0 207 179 257 1302.2 0 257 180 347 1302.2 0 347 181 336 1302.2 0 336 182 279 1302.2 0 279 183 413 1302.2 0 413 184 535 1302.2 0 535 185 463 1302.2 0 463 186 280 1302.2 0 280 187 66 1302.2 0 66 188 140 1302.2 0 140 189 240 1302.2 0 240 190 250 1302.2 0 250 191 155 1302.2 0 155 192 296 1302.2 0 296 193 206 1302.2 0 206 194 110 1302.2 0 110 195 134 1302.2 0 134 196 321 1302.2 0 321 197 204 1302.2 0 204 198 102 1302.2 0 102 199 121 1302.2 0 121 200 446 1302.2 0 446 201 336 1302.2 0 336 202 261 1302.2 0 261 203 147 1302.2 0 147 204 142 1302.2 0 142 205 225 1302.2 0 225 206 173 1302.2 0 173 207 183 1302.2 0 183 208 282 1302.2 0 282 209 403 1302.2 0 403 210 264 1302.2 0 264 211 150 1302.2 0 150 212 248 1302.2 0 248 213 238 1302.2 0 238 214 205 1302.2 0 205 215 355 1302.2 0 355 216 370 1302.2 0 370 217 460 1302.2 0 460 218 622 1302.2 0 622 219 9428 1302.2 0 9428 220 1191 1302.2 0 1191 221 3270 1302.2 0 3270 222 417 1302.2 0 417 223 214 1302.2 0 214 224 192 1302.2 0 192 225 261 1302.2 0 261 226 378 1302.2 0 378 227 270 1302.2 0 270 228 189 1302.2 0 189 229 169 1302.2 0 169 230 144 1302.2 0 144 231 90 1302.2 0 90 232 223 1302.2 0 223 233 301 1302.2 0 301 234 165 1302.2 0 165 235 94 1302.2 0 94 236 154 1302.2 0 154 237 163 1302.2 0 163 238 131 1302.2 0 131 239 128 1302.2 0 128 240 218 1302.2 0 218 241 151 1302.2 0 151 242 173 1302.2 0 173 243 136 1302.2 0 136 244 163 1302.2 0 163 245 229 1302.2 0 229 246 114 1302.2 0 114 247 118 1302.2 0 118 248 162 1302.2 0 162 249 515 1302.2 0 515 250 1476 1302.2 0 1476 251 737 1302.2 0 737 252 203 1302.2 0 203 253 237 1302.2 0 237 254 532 1302.2 0 532 255 357 1302.2 0 357 256 316 1302.2 0 316 257 259 1302.2 0 259 258 187 1302.2 0 187 259 129 1302.2 0 129 260 111 1302.2 0 111 261 85 1302.2 0 85 262 132 1302.2 0 132 263 195 1302.2 0 195 264 284 1302.2 0 284 265 253 1302.2 0 253 266 161 1302.2 0 161 267 90 1302.2 0 90 268 88 1302.2 0 88 269 198 1302.2 0 198 270 172 1302.2 0 172 271 151 1302.2 0 151 272 136 1302.2 0 136 273 95 1302.2 0 95 274 79 1302.2 0 79 275 91 1302.2 0 91 276 116 1302.2 0 116 277 197 1302.2 0 197 278 200 1302.2 0 200 279 313 1302.2 0 313 280 270 1302.2 0 270 281 298 1302.2 0 298 282 214 1302.2 0 214 283 156 1302.2 0 156 284 203 1302.2 0 203 285 256 1302.2 0 256 286 134 1302.2 0 134 287 103 1302.2 0 103 288 118 1302.2 0 118 289 119 1302.2 0 119 290 193 1302.2 0 193 291 235 1302.2 0 235 292 216 1302.2 0 216 293 124 1302.2 0 124 294 121 1302.2 0 121 295 125 1302.2 0 125 296 96 1302.2 0 96 297 220 1302.2 0 220 298 170 1302.2 0 170 299 61 1302.2 0 61 300 40 1302.2 0 40 301 71 1302.2 0 71 Finished in 2.65 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 54,726 Reads with adapters: 9,885 (18.1%) Reads that were too short: 31 (0.1%) Reads written (passing filters): 9,854 (18.0%) Total basepairs processed: 12,996,997 bp Total written (filtered): 2,704,433 bp (20.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9885 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 7 0.0 3 0 0 5 2 16 123 0.0 3 0 71 6 46 17 9444 0.0 3 9108 289 33 14 18 102 0.0 3 1 17 78 6 19 125 0.0 3 0 0 5 120 20 5 0.0 3 0 0 0 5 24 1 0.0 3 0 0 0 1 30 1 0.0 3 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 54 8 0.0 3 0 0 0 8 57 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 109 3 0.0 3 0 0 0 3 110 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 120 3 0.0 3 0 0 0 3 122 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 3 0.0 3 0 0 0 3 283 2 0.0 3 0 0 0 2 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.44 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 9,854 Reads with adapters: 302 (3.1%) Reads written (passing filters): 9,854 (100.0%) Total basepairs processed: 2,704,433 bp Total written (filtered): 2,703,476 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 302 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.5% C: 19.9% G: 12.9% T: 49.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 252 154.0 0 252 4 49 38.5 0 49 5 1 9.6 0 1