This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 167.78 s (31 us/read; 1.91 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 206,298 (3.9%) Reads that were too short: 24,609 (0.5%) Reads written (passing filters): 181,689 (3.4%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 38,311,841 bp (2.4%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 206298 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4295 1302.2 0 4295 7 476 1302.2 0 476 8 1416 1302.2 0 1416 9 3179 1302.2 0 3179 10 3517 1302.2 0 3517 11 18 1302.2 0 18 12 109 1302.2 0 109 13 13 1302.2 0 13 14 4 1302.2 0 4 15 9 1302.2 0 9 16 12 1302.2 0 12 17 15 1302.2 0 15 18 12 1302.2 0 12 19 11 1302.2 0 11 20 5 1302.2 0 5 21 7 1302.2 0 7 22 33 1302.2 0 33 23 17 1302.2 0 17 24 7 1302.2 0 7 25 6 1302.2 0 6 26 11 1302.2 0 11 27 10 1302.2 0 10 28 9 1302.2 0 9 29 5 1302.2 0 5 30 9 1302.2 0 9 31 15 1302.2 0 15 32 15 1302.2 0 15 33 13 1302.2 0 13 34 7 1302.2 0 7 35 17 1302.2 0 17 36 10 1302.2 0 10 37 30 1302.2 0 30 38 21 1302.2 0 21 39 37 1302.2 0 37 40 113 1302.2 0 113 41 238 1302.2 0 238 42 35 1302.2 0 35 43 13 1302.2 0 13 44 11 1302.2 0 11 45 16 1302.2 0 16 46 11 1302.2 0 11 47 11 1302.2 0 11 48 9 1302.2 0 9 49 16 1302.2 0 16 50 15 1302.2 0 15 51 40 1302.2 0 40 52 34 1302.2 0 34 53 23 1302.2 0 23 54 28 1302.2 0 28 55 14 1302.2 0 14 56 15 1302.2 0 15 57 25 1302.2 0 25 58 22 1302.2 0 22 59 13 1302.2 0 13 60 46 1302.2 0 46 61 29 1302.2 0 29 62 23 1302.2 0 23 63 76 1302.2 0 76 64 20 1302.2 0 20 65 44 1302.2 0 44 66 43 1302.2 0 43 67 30 1302.2 0 30 68 58 1302.2 0 58 69 25 1302.2 0 25 70 30 1302.2 0 30 71 16 1302.2 0 16 72 71 1302.2 0 71 73 59 1302.2 0 59 74 66 1302.2 0 66 75 145 1302.2 0 145 76 193 1302.2 0 193 77 192 1302.2 0 192 78 370 1302.2 0 370 79 1080 1302.2 0 1080 80 281 1302.2 0 281 81 511 1302.2 0 511 82 896 1302.2 0 896 83 495 1302.2 0 495 84 622 1302.2 0 622 85 1072 1302.2 0 1072 86 992 1302.2 0 992 87 1802 1302.2 0 1802 88 772 1302.2 0 772 89 865 1302.2 0 865 90 1128 1302.2 0 1128 91 1359 1302.2 0 1359 92 12221 1302.2 0 12221 93 67876 1302.2 0 67876 94 64603 1302.2 0 64603 95 695 1302.2 0 695 96 72 1302.2 0 72 97 19 1302.2 0 19 98 23 1302.2 0 23 99 19 1302.2 0 19 100 18 1302.2 0 18 101 17 1302.2 0 17 102 20 1302.2 0 20 103 3 1302.2 0 3 104 7 1302.2 0 7 105 21 1302.2 0 21 106 25 1302.2 0 25 107 130 1302.2 0 130 108 81 1302.2 0 81 109 11 1302.2 0 11 110 18 1302.2 0 18 111 77 1302.2 0 77 112 73 1302.2 0 73 113 9 1302.2 0 9 114 72 1302.2 0 72 115 113 1302.2 0 113 116 128 1302.2 0 128 117 57 1302.2 0 57 118 20 1302.2 0 20 119 26 1302.2 0 26 120 33 1302.2 0 33 121 22 1302.2 0 22 122 23 1302.2 0 23 123 12 1302.2 0 12 124 34 1302.2 0 34 125 24 1302.2 0 24 126 22 1302.2 0 22 127 34 1302.2 0 34 128 25 1302.2 0 25 129 32 1302.2 0 32 130 78 1302.2 0 78 131 84 1302.2 0 84 132 28 1302.2 0 28 133 28 1302.2 0 28 134 26 1302.2 0 26 135 36 1302.2 0 36 136 39 1302.2 0 39 137 54 1302.2 0 54 138 69 1302.2 0 69 139 97 1302.2 0 97 140 78 1302.2 0 78 141 108 1302.2 0 108 142 24 1302.2 0 24 143 36 1302.2 0 36 144 91 1302.2 0 91 145 32 1302.2 0 32 146 25 1302.2 0 25 147 22 1302.2 0 22 148 24 1302.2 0 24 149 17 1302.2 0 17 150 17 1302.2 0 17 151 10 1302.2 0 10 152 9 1302.2 0 9 153 15 1302.2 0 15 154 14 1302.2 0 14 155 8 1302.2 0 8 156 46 1302.2 0 46 157 22 1302.2 0 22 158 45 1302.2 0 45 159 24 1302.2 0 24 160 20 1302.2 0 20 161 18 1302.2 0 18 162 11 1302.2 0 11 163 16 1302.2 0 16 164 29 1302.2 0 29 165 36 1302.2 0 36 166 34 1302.2 0 34 167 23 1302.2 0 23 168 24 1302.2 0 24 169 45 1302.2 0 45 170 18 1302.2 0 18 171 29 1302.2 0 29 172 32 1302.2 0 32 173 25 1302.2 0 25 174 21 1302.2 0 21 175 47 1302.2 0 47 176 37 1302.2 0 37 177 228 1302.2 0 228 178 370 1302.2 0 370 179 741 1302.2 0 741 180 154 1302.2 0 154 181 27 1302.2 0 27 182 29 1302.2 0 29 183 27 1302.2 0 27 184 24 1302.2 0 24 185 49 1302.2 0 49 186 51 1302.2 0 51 187 39 1302.2 0 39 188 120 1302.2 0 120 189 53 1302.2 0 53 190 135 1302.2 0 135 191 126 1302.2 0 126 192 70 1302.2 0 70 193 46 1302.2 0 46 194 67 1302.2 0 67 195 69 1302.2 0 69 196 67 1302.2 0 67 197 94 1302.2 0 94 198 123 1302.2 0 123 199 142 1302.2 0 142 200 202 1302.2 0 202 201 2767 1302.2 0 2767 202 5461 1302.2 0 5461 203 6911 1302.2 0 6911 204 535 1302.2 0 535 205 255 1302.2 0 255 206 541 1302.2 0 541 207 121 1302.2 0 121 208 164 1302.2 0 164 209 77 1302.2 0 77 210 46 1302.2 0 46 211 60 1302.2 0 60 212 53 1302.2 0 53 213 44 1302.2 0 44 214 81 1302.2 0 81 215 34 1302.2 0 34 216 62 1302.2 0 62 217 75 1302.2 0 75 218 114 1302.2 0 114 219 50 1302.2 0 50 220 41 1302.2 0 41 221 38 1302.2 0 38 222 74 1302.2 0 74 223 139 1302.2 0 139 224 47 1302.2 0 47 225 187 1302.2 0 187 226 333 1302.2 0 333 227 517 1302.2 0 517 228 440 1302.2 0 440 229 265 1302.2 0 265 230 27 1302.2 0 27 231 15 1302.2 0 15 232 18 1302.2 0 18 233 16 1302.2 0 16 234 11 1302.2 0 11 235 19 1302.2 0 19 236 46 1302.2 0 46 237 45 1302.2 0 45 238 55 1302.2 0 55 239 5 1302.2 0 5 240 1 1302.2 0 1 241 6 1302.2 0 6 242 6 1302.2 0 6 243 10 1302.2 0 10 244 12 1302.2 0 12 245 31 1302.2 0 31 246 53 1302.2 0 53 247 19 1302.2 0 19 248 11 1302.2 0 11 249 6 1302.2 0 6 250 8 1302.2 0 8 251 7 1302.2 0 7 252 17 1302.2 0 17 253 13 1302.2 0 13 254 44 1302.2 0 44 255 99 1302.2 0 99 256 39 1302.2 0 39 257 22 1302.2 0 22 258 50 1302.2 0 50 259 57 1302.2 0 57 260 94 1302.2 0 94 261 59 1302.2 0 59 262 44 1302.2 0 44 263 21 1302.2 0 21 264 7 1302.2 0 7 265 23 1302.2 0 23 266 48 1302.2 0 48 267 26 1302.2 0 26 268 14 1302.2 0 14 269 40 1302.2 0 40 270 33 1302.2 0 33 271 21 1302.2 0 21 272 57 1302.2 0 57 273 37 1302.2 0 37 274 13 1302.2 0 13 275 14 1302.2 0 14 276 5 1302.2 0 5 277 18 1302.2 0 18 278 56 1302.2 0 56 279 63 1302.2 0 63 280 70 1302.2 0 70 281 60 1302.2 0 60 282 49 1302.2 0 49 283 18 1302.2 0 18 284 48 1302.2 0 48 285 157 1302.2 0 157 286 494 1302.2 0 494 287 4862 1302.2 0 4862 288 18 1302.2 0 18 289 7 1302.2 0 7 290 15 1302.2 0 15 291 16 1302.2 0 16 292 107 1302.2 0 107 293 306 1302.2 0 306 294 35 1302.2 0 35 295 36 1302.2 0 36 296 15 1302.2 0 15 297 9 1302.2 0 9 298 18 1302.2 0 18 299 19 1302.2 0 19 300 11 1302.2 0 11 301 13 1302.2 0 13 Finished in 7.95 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 181,689 Reads with adapters: 2,398 (1.3%) Reads that were too short: 112 (0.1%) Reads written (passing filters): 2,286 (1.3%) Total basepairs processed: 38,311,841 bp Total written (filtered): 446,706 bp (1.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2398 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 19 0.0 3 0 8 0 11 17 1088 0.0 3 1050 32 4 2 18 34 0.0 3 1 1 28 4 19 30 0.0 3 2 1 7 20 20 3 0.0 3 0 0 1 2 22 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 27 1 0.0 3 1 28 2 0.0 3 2 30 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 74 14 0.0 3 0 0 0 14 75 1 0.0 3 0 0 0 1 76 5 0.0 3 0 0 0 5 77 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 79 9 0.0 3 0 0 0 9 80 4 0.0 3 0 0 0 4 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 1 84 3 0.0 3 0 0 1 2 90 1 0.0 3 0 0 0 1 98 9 0.0 3 0 0 0 9 99 667 0.0 3 0 0 2 665 100 100 0.0 3 0 0 0 100 101 6 0.0 3 0 0 0 6 102 243 0.0 3 0 0 5 238 103 29 0.0 3 0 0 1 28 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 125 5 0.0 3 0 0 0 5 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 13 0.0 3 0 0 0 13 162 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 185 3 0.0 3 0 0 0 3 186 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 191 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 200 36 0.0 3 0 0 1 35 201 20 0.0 3 0 0 0 20 202 3 0.0 3 0 0 0 3 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 2,286 Reads with adapters: 100 (4.4%) Reads written (passing filters): 2,286 (100.0%) Total basepairs processed: 446,706 bp Total written (filtered): 446,391 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 100 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.0% C: 2.0% G: 36.0% T: 33.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 85 35.7 0 85 4 15 8.9 0 15