This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 174.11 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 513,916 (9.6%) Reads that were too short: 228,197 (4.3%) Reads written (passing filters): 285,719 (5.4%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 49,184,073 bp (3.1%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 513916 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 417 1302.2 0 417 7 23 1302.2 0 23 8 273 1302.2 0 273 9 1141 1302.2 0 1141 10 23 1302.2 0 23 11 8 1302.2 0 8 12 17 1302.2 0 17 13 16 1302.2 0 16 14 8 1302.2 0 8 15 15 1302.2 0 15 16 16 1302.2 0 16 17 5 1302.2 0 5 18 15 1302.2 0 15 19 12 1302.2 0 12 20 8 1302.2 0 8 21 7 1302.2 0 7 22 11 1302.2 0 11 23 7 1302.2 0 7 24 13 1302.2 0 13 25 26 1302.2 0 26 26 9 1302.2 0 9 27 9 1302.2 0 9 28 8 1302.2 0 8 29 11 1302.2 0 11 30 35 1302.2 0 35 31 23 1302.2 0 23 32 24 1302.2 0 24 33 2665 1302.2 0 2665 34 926 1302.2 0 926 35 20 1302.2 0 20 36 16 1302.2 0 16 37 12 1302.2 0 12 38 21 1302.2 0 21 39 27 1302.2 0 27 40 13 1302.2 0 13 41 30 1302.2 0 30 42 96 1302.2 0 96 43 25 1302.2 0 25 44 5 1302.2 0 5 45 13 1302.2 0 13 46 13 1302.2 0 13 47 11 1302.2 0 11 48 14 1302.2 0 14 49 13 1302.2 0 13 50 21 1302.2 0 21 51 44 1302.2 0 44 52 135 1302.2 0 135 53 86 1302.2 0 86 54 189 1302.2 0 189 55 28 1302.2 0 28 56 21 1302.2 0 21 57 41 1302.2 0 41 58 28 1302.2 0 28 59 28 1302.2 0 28 60 21 1302.2 0 21 61 19 1302.2 0 19 62 53 1302.2 0 53 63 125 1302.2 0 125 64 231 1302.2 0 231 65 450 1302.2 0 450 66 468 1302.2 0 468 67 928 1302.2 0 928 68 2866 1302.2 0 2866 69 52025 1302.2 0 52025 70 1152 1302.2 0 1152 71 6564 1302.2 0 6564 72 61 1302.2 0 61 73 45 1302.2 0 45 74 8 1302.2 0 8 75 11 1302.2 0 11 76 13 1302.2 0 13 77 21 1302.2 0 21 78 21 1302.2 0 21 79 49 1302.2 0 49 80 617 1302.2 0 617 81 1058 1302.2 0 1058 82 1657 1302.2 0 1657 83 353 1302.2 0 353 84 1743 1302.2 0 1743 85 1584 1302.2 0 1584 86 689 1302.2 0 689 87 314 1302.2 0 314 88 983 1302.2 0 983 89 132 1302.2 0 132 90 84 1302.2 0 84 91 355 1302.2 0 355 92 940 1302.2 0 940 93 197 1302.2 0 197 94 120 1302.2 0 120 95 525 1302.2 0 525 96 1258 1302.2 0 1258 97 1356 1302.2 0 1356 98 1199 1302.2 0 1199 99 425 1302.2 0 425 100 354 1302.2 0 354 101 1519 1302.2 0 1519 102 11563 1302.2 0 11563 103 2290 1302.2 0 2290 104 2424 1302.2 0 2424 105 921 1302.2 0 921 106 270 1302.2 0 270 107 443 1302.2 0 443 108 1615 1302.2 0 1615 109 520 1302.2 0 520 110 173 1302.2 0 173 111 511 1302.2 0 511 112 904 1302.2 0 904 113 621 1302.2 0 621 114 1118 1302.2 0 1118 115 460 1302.2 0 460 116 378 1302.2 0 378 117 880 1302.2 0 880 118 930 1302.2 0 930 119 922 1302.2 0 922 120 1683 1302.2 0 1683 121 1552 1302.2 0 1552 122 1497 1302.2 0 1497 123 1746 1302.2 0 1746 124 1768 1302.2 0 1768 125 1112 1302.2 0 1112 126 1374 1302.2 0 1374 127 1079 1302.2 0 1079 128 991 1302.2 0 991 129 1356 1302.2 0 1356 130 867 1302.2 0 867 131 982 1302.2 0 982 132 1946 1302.2 0 1946 133 3533 1302.2 0 3533 134 3105 1302.2 0 3105 135 1478 1302.2 0 1478 136 2037 1302.2 0 2037 137 2583 1302.2 0 2583 138 4280 1302.2 0 4280 139 2664 1302.2 0 2664 140 2635 1302.2 0 2635 141 1318 1302.2 0 1318 142 1706 1302.2 0 1706 143 2227 1302.2 0 2227 144 1051 1302.2 0 1051 145 790 1302.2 0 790 146 1062 1302.2 0 1062 147 2259 1302.2 0 2259 148 3668 1302.2 0 3668 149 2001 1302.2 0 2001 150 1895 1302.2 0 1895 151 1160 1302.2 0 1160 152 1145 1302.2 0 1145 153 930 1302.2 0 930 154 736 1302.2 0 736 155 1089 1302.2 0 1089 156 1450 1302.2 0 1450 157 1393 1302.2 0 1393 158 2248 1302.2 0 2248 159 2709 1302.2 0 2709 160 2490 1302.2 0 2490 161 1982 1302.2 0 1982 162 1980 1302.2 0 1980 163 1932 1302.2 0 1932 164 1671 1302.2 0 1671 165 1239 1302.2 0 1239 166 1530 1302.2 0 1530 167 1774 1302.2 0 1774 168 2959 1302.2 0 2959 169 2880 1302.2 0 2880 170 1662 1302.2 0 1662 171 2455 1302.2 0 2455 172 2885 1302.2 0 2885 173 1102 1302.2 0 1102 174 1137 1302.2 0 1137 175 2515 1302.2 0 2515 176 7371 1302.2 0 7371 177 5413 1302.2 0 5413 178 2989 1302.2 0 2989 179 1284 1302.2 0 1284 180 1029 1302.2 0 1029 181 1431 1302.2 0 1431 182 2007 1302.2 0 2007 183 2229 1302.2 0 2229 184 2139 1302.2 0 2139 185 2081 1302.2 0 2081 186 2427 1302.2 0 2427 187 3366 1302.2 0 3366 188 5014 1302.2 0 5014 189 5925 1302.2 0 5925 190 1185 1302.2 0 1185 191 735 1302.2 0 735 192 796 1302.2 0 796 193 1956 1302.2 0 1956 194 1912 1302.2 0 1912 195 2496 1302.2 0 2496 196 3440 1302.2 0 3440 197 1324 1302.2 0 1324 198 886 1302.2 0 886 199 1383 1302.2 0 1383 200 2494 1302.2 0 2494 201 2852 1302.2 0 2852 202 1323 1302.2 0 1323 203 733 1302.2 0 733 204 1131 1302.2 0 1131 205 1780 1302.2 0 1780 206 1838 1302.2 0 1838 207 2074 1302.2 0 2074 208 2784 1302.2 0 2784 209 1672 1302.2 0 1672 210 1468 1302.2 0 1468 211 1552 1302.2 0 1552 212 1751 1302.2 0 1751 213 2825 1302.2 0 2825 214 2535 1302.2 0 2535 215 3188 1302.2 0 3188 216 2574 1302.2 0 2574 217 2273 1302.2 0 2273 218 2276 1302.2 0 2276 219 3239 1302.2 0 3239 220 2055 1302.2 0 2055 221 2973 1302.2 0 2973 222 2908 1302.2 0 2908 223 1603 1302.2 0 1603 224 1951 1302.2 0 1951 225 2683 1302.2 0 2683 226 2127 1302.2 0 2127 227 1842 1302.2 0 1842 228 1836 1302.2 0 1836 229 2605 1302.2 0 2605 230 2896 1302.2 0 2896 231 2179 1302.2 0 2179 232 1986 1302.2 0 1986 233 2407 1302.2 0 2407 234 934 1302.2 0 934 235 889 1302.2 0 889 236 1712 1302.2 0 1712 237 2518 1302.2 0 2518 238 2737 1302.2 0 2737 239 2638 1302.2 0 2638 240 2355 1302.2 0 2355 241 1987 1302.2 0 1987 242 1634 1302.2 0 1634 243 1526 1302.2 0 1526 244 2659 1302.2 0 2659 245 2320 1302.2 0 2320 246 2730 1302.2 0 2730 247 1987 1302.2 0 1987 248 2311 1302.2 0 2311 249 2574 1302.2 0 2574 250 2111 1302.2 0 2111 251 981 1302.2 0 981 252 799 1302.2 0 799 253 1451 1302.2 0 1451 254 2612 1302.2 0 2612 255 2707 1302.2 0 2707 256 2701 1302.2 0 2701 257 2140 1302.2 0 2140 258 2742 1302.2 0 2742 259 3305 1302.2 0 3305 260 1854 1302.2 0 1854 261 1599 1302.2 0 1599 262 1157 1302.2 0 1157 263 1398 1302.2 0 1398 264 2227 1302.2 0 2227 265 2700 1302.2 0 2700 266 3166 1302.2 0 3166 267 2948 1302.2 0 2948 268 3006 1302.2 0 3006 269 4547 1302.2 0 4547 270 3582 1302.2 0 3582 271 2617 1302.2 0 2617 272 1457 1302.2 0 1457 273 1490 1302.2 0 1490 274 1989 1302.2 0 1989 275 2872 1302.2 0 2872 276 4439 1302.2 0 4439 277 3671 1302.2 0 3671 278 2581 1302.2 0 2581 279 1636 1302.2 0 1636 280 1606 1302.2 0 1606 281 3822 1302.2 0 3822 282 4191 1302.2 0 4191 283 2512 1302.2 0 2512 284 1842 1302.2 0 1842 285 1823 1302.2 0 1823 286 2129 1302.2 0 2129 287 2736 1302.2 0 2736 288 3392 1302.2 0 3392 289 3294 1302.2 0 3294 290 2394 1302.2 0 2394 291 1523 1302.2 0 1523 292 1398 1302.2 0 1398 293 1649 1302.2 0 1649 294 2952 1302.2 0 2952 295 4001 1302.2 0 4001 296 2271 1302.2 0 2271 297 1252 1302.2 0 1252 298 1515 1302.2 0 1515 299 1856 1302.2 0 1856 300 1663 1302.2 0 1663 301 3313 1302.2 0 3313 Finished in 12.67 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 285,719 Reads with adapters: 1,231 (0.4%) Reads that were too short: 65 (0.0%) Reads written (passing filters): 1,166 (0.4%) Total basepairs processed: 49,184,073 bp Total written (filtered): 313,553 bp (0.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1231 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 2 0.0 3 0 0 0 0 2 19 23 0.0 3 0 20 1 0 2 20 1093 0.0 4 1078 8 1 2 4 21 10 0.0 4 4 4 0 1 1 22 13 0.0 4 6 0 0 1 6 24 2 0.0 4 0 0 0 0 2 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 28 2 0.0 4 0 0 0 0 2 29 1 0.0 4 0 0 0 0 1 30 2 0.0 4 0 0 0 0 2 32 1 0.0 4 0 0 0 0 1 34 2 0.0 4 0 0 0 0 2 35 1 0.0 4 0 0 0 0 1 38 3 0.0 4 0 0 0 0 3 41 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 52 3 0.0 4 0 0 0 0 3 54 2 0.0 4 0 0 0 0 2 55 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 60 3 0.0 4 0 0 0 0 3 64 2 0.0 4 0 0 0 0 2 65 2 0.0 4 0 0 0 0 2 68 2 0.0 4 0 0 0 0 2 74 3 0.0 4 0 0 0 0 3 75 2 0.0 4 0 0 0 0 2 76 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 78 3 0.0 4 0 0 0 0 3 80 2 0.0 4 0 0 0 0 2 87 1 0.0 4 0 0 0 0 1 90 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 94 3 0.0 4 0 0 0 0 3 97 2 0.0 4 0 0 0 0 2 98 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 0 0 2 104 1 0.0 4 0 0 0 0 1 107 2 0.0 4 0 0 0 0 2 109 2 0.0 4 0 0 0 0 2 110 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 119 2 0.0 4 0 0 0 1 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 2 0.0 4 0 0 0 0 2 132 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 164 2 0.0 4 0 0 0 0 2 165 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.05 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 1,166 Reads with adapters: 16 (1.4%) Reads written (passing filters): 1,166 (100.0%) Total basepairs processed: 313,553 bp Total written (filtered): 311,854 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 16 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 12.5% C: 0.0% G: 62.5% T: 18.8% none/other: 6.2% Overview of removed sequences length count expect max.err error counts 10 4 0.0 1 0 4 11 6 0.0 1 0 6 257 1 0.0 1 1 261 1 0.0 1 1 265 1 0.0 1 1 267 1 0.0 1 1 271 1 0.0 1 1 272 1 0.0 1 0 1