This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 181.03 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 1,039,569 (19.5%) Reads that were too short: 363,611 (6.8%) Reads written (passing filters): 675,958 (12.7%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 118,349,953 bp (7.4%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 1039569 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 235 1302.2 0 235 7 372 1302.2 0 372 8 4821 1302.2 0 4821 9 26010 1302.2 0 26010 10 57 1302.2 0 57 11 31 1302.2 0 31 12 17 1302.2 0 17 13 6 1302.2 0 6 14 15 1302.2 0 15 15 28 1302.2 0 28 16 20 1302.2 0 20 17 7 1302.2 0 7 18 12 1302.2 0 12 19 22 1302.2 0 22 20 13 1302.2 0 13 21 17 1302.2 0 17 22 12 1302.2 0 12 23 30 1302.2 0 30 24 58 1302.2 0 58 25 24 1302.2 0 24 26 13 1302.2 0 13 27 16 1302.2 0 16 28 39 1302.2 0 39 29 32 1302.2 0 32 30 191 1302.2 0 191 31 65 1302.2 0 65 32 20 1302.2 0 20 33 21 1302.2 0 21 34 18 1302.2 0 18 35 22 1302.2 0 22 36 12 1302.2 0 12 37 105 1302.2 0 105 38 21 1302.2 0 21 39 11 1302.2 0 11 40 12 1302.2 0 12 41 21 1302.2 0 21 42 21 1302.2 0 21 43 11 1302.2 0 11 44 18 1302.2 0 18 45 33 1302.2 0 33 46 61 1302.2 0 61 47 68 1302.2 0 68 48 21 1302.2 0 21 49 17 1302.2 0 17 50 22 1302.2 0 22 51 27 1302.2 0 27 52 25 1302.2 0 25 53 13 1302.2 0 13 54 10 1302.2 0 10 55 14 1302.2 0 14 56 12 1302.2 0 12 57 16 1302.2 0 16 58 199 1302.2 0 199 59 78 1302.2 0 78 60 26 1302.2 0 26 61 13 1302.2 0 13 62 61 1302.2 0 61 63 41 1302.2 0 41 64 48 1302.2 0 48 65 31 1302.2 0 31 66 32 1302.2 0 32 67 31 1302.2 0 31 68 106 1302.2 0 106 69 62 1302.2 0 62 70 93 1302.2 0 93 71 331 1302.2 0 331 72 4493 1302.2 0 4493 73 2756 1302.2 0 2756 74 880 1302.2 0 880 75 845 1302.2 0 845 76 3087 1302.2 0 3087 77 10997 1302.2 0 10997 78 5327 1302.2 0 5327 79 7084 1302.2 0 7084 80 11915 1302.2 0 11915 81 12598 1302.2 0 12598 82 8384 1302.2 0 8384 83 8177 1302.2 0 8177 84 17166 1302.2 0 17166 85 12392 1302.2 0 12392 86 9711 1302.2 0 9711 87 1988 1302.2 0 1988 88 1429 1302.2 0 1429 89 4399 1302.2 0 4399 90 4142 1302.2 0 4142 91 1432 1302.2 0 1432 92 1886 1302.2 0 1886 93 5827 1302.2 0 5827 94 8634 1302.2 0 8634 95 14670 1302.2 0 14670 96 6786 1302.2 0 6786 97 1635 1302.2 0 1635 98 1676 1302.2 0 1676 99 6935 1302.2 0 6935 100 10727 1302.2 0 10727 101 6303 1302.2 0 6303 102 2677 1302.2 0 2677 103 2253 1302.2 0 2253 104 862 1302.2 0 862 105 2476 1302.2 0 2476 106 7989 1302.2 0 7989 107 1608 1302.2 0 1608 108 567 1302.2 0 567 109 1722 1302.2 0 1722 110 4410 1302.2 0 4410 111 4343 1302.2 0 4343 112 4684 1302.2 0 4684 113 4034 1302.2 0 4034 114 3936 1302.2 0 3936 115 7441 1302.2 0 7441 116 4548 1302.2 0 4548 117 4150 1302.2 0 4150 118 9424 1302.2 0 9424 119 7394 1302.2 0 7394 120 5317 1302.2 0 5317 121 3592 1302.2 0 3592 122 3260 1302.2 0 3260 123 3625 1302.2 0 3625 124 4684 1302.2 0 4684 125 5398 1302.2 0 5398 126 6563 1302.2 0 6563 127 7163 1302.2 0 7163 128 3886 1302.2 0 3886 129 3005 1302.2 0 3005 130 5546 1302.2 0 5546 131 8369 1302.2 0 8369 132 6133 1302.2 0 6133 133 4076 1302.2 0 4076 134 4134 1302.2 0 4134 135 4520 1302.2 0 4520 136 6628 1302.2 0 6628 137 7393 1302.2 0 7393 138 5789 1302.2 0 5789 139 4994 1302.2 0 4994 140 4776 1302.2 0 4776 141 7098 1302.2 0 7098 142 3713 1302.2 0 3713 143 3228 1302.2 0 3228 144 4157 1302.2 0 4157 145 7316 1302.2 0 7316 146 5311 1302.2 0 5311 147 4017 1302.2 0 4017 148 4396 1302.2 0 4396 149 4624 1302.2 0 4624 150 3460 1302.2 0 3460 151 2836 1302.2 0 2836 152 4072 1302.2 0 4072 153 5724 1302.2 0 5724 154 6040 1302.2 0 6040 155 3037 1302.2 0 3037 156 3060 1302.2 0 3060 157 5287 1302.2 0 5287 158 4955 1302.2 0 4955 159 4307 1302.2 0 4307 160 3559 1302.2 0 3559 161 3013 1302.2 0 3013 162 3760 1302.2 0 3760 163 3662 1302.2 0 3662 164 3533 1302.2 0 3533 165 3892 1302.2 0 3892 166 3559 1302.2 0 3559 167 3238 1302.2 0 3238 168 3660 1302.2 0 3660 169 4997 1302.2 0 4997 170 4910 1302.2 0 4910 171 2472 1302.2 0 2472 172 2170 1302.2 0 2170 173 4933 1302.2 0 4933 174 6779 1302.2 0 6779 175 3472 1302.2 0 3472 176 2139 1302.2 0 2139 177 2027 1302.2 0 2027 178 2923 1302.2 0 2923 179 5194 1302.2 0 5194 180 5326 1302.2 0 5326 181 3854 1302.2 0 3854 182 6030 1302.2 0 6030 183 4786 1302.2 0 4786 184 6661 1302.2 0 6661 185 5259 1302.2 0 5259 186 5046 1302.2 0 5046 187 3842 1302.2 0 3842 188 2420 1302.2 0 2420 189 1926 1302.2 0 1926 190 3425 1302.2 0 3425 191 6326 1302.2 0 6326 192 5887 1302.2 0 5887 193 5597 1302.2 0 5597 194 4760 1302.2 0 4760 195 2472 1302.2 0 2472 196 1617 1302.2 0 1617 197 4225 1302.2 0 4225 198 12080 1302.2 0 12080 199 8589 1302.2 0 8589 200 2426 1302.2 0 2426 201 1257 1302.2 0 1257 202 2569 1302.2 0 2569 203 5878 1302.2 0 5878 204 5518 1302.2 0 5518 205 4880 1302.2 0 4880 206 5863 1302.2 0 5863 207 3194 1302.2 0 3194 208 2019 1302.2 0 2019 209 2748 1302.2 0 2748 210 4271 1302.2 0 4271 211 5505 1302.2 0 5505 212 3604 1302.2 0 3604 213 3491 1302.2 0 3491 214 3782 1302.2 0 3782 215 3333 1302.2 0 3333 216 2752 1302.2 0 2752 217 3593 1302.2 0 3593 218 4509 1302.2 0 4509 219 4791 1302.2 0 4791 220 3189 1302.2 0 3189 221 1578 1302.2 0 1578 222 2220 1302.2 0 2220 223 3876 1302.2 0 3876 224 3462 1302.2 0 3462 225 2531 1302.2 0 2531 226 2921 1302.2 0 2921 227 3821 1302.2 0 3821 228 3306 1302.2 0 3306 229 2965 1302.2 0 2965 230 3675 1302.2 0 3675 231 4105 1302.2 0 4105 232 2236 1302.2 0 2236 233 2443 1302.2 0 2443 234 5929 1302.2 0 5929 235 7270 1302.2 0 7270 236 4436 1302.2 0 4436 237 3464 1302.2 0 3464 238 4184 1302.2 0 4184 239 4507 1302.2 0 4507 240 3764 1302.2 0 3764 241 3147 1302.2 0 3147 242 3398 1302.2 0 3398 243 3670 1302.2 0 3670 244 2801 1302.2 0 2801 245 2482 1302.2 0 2482 246 3643 1302.2 0 3643 247 3889 1302.2 0 3889 248 4201 1302.2 0 4201 249 2793 1302.2 0 2793 250 1926 1302.2 0 1926 251 2114 1302.2 0 2114 252 3404 1302.2 0 3404 253 3427 1302.2 0 3427 254 2222 1302.2 0 2222 255 2236 1302.2 0 2236 256 2261 1302.2 0 2261 257 2925 1302.2 0 2925 258 2660 1302.2 0 2660 259 2700 1302.2 0 2700 260 3071 1302.2 0 3071 261 4237 1302.2 0 4237 262 2815 1302.2 0 2815 263 2251 1302.2 0 2251 264 2474 1302.2 0 2474 265 3092 1302.2 0 3092 266 3107 1302.2 0 3107 267 4699 1302.2 0 4699 268 3259 1302.2 0 3259 269 2287 1302.2 0 2287 270 2562 1302.2 0 2562 271 3598 1302.2 0 3598 272 3987 1302.2 0 3987 273 5302 1302.2 0 5302 274 4939 1302.2 0 4939 275 3235 1302.2 0 3235 276 2280 1302.2 0 2280 277 3258 1302.2 0 3258 278 4024 1302.2 0 4024 279 4350 1302.2 0 4350 280 3343 1302.2 0 3343 281 2431 1302.2 0 2431 282 2514 1302.2 0 2514 283 2216 1302.2 0 2216 284 2473 1302.2 0 2473 285 3395 1302.2 0 3395 286 3900 1302.2 0 3900 287 2714 1302.2 0 2714 288 4060 1302.2 0 4060 289 4540 1302.2 0 4540 290 4051 1302.2 0 4051 291 3522 1302.2 0 3522 292 4228 1302.2 0 4228 293 4659 1302.2 0 4659 294 3947 1302.2 0 3947 295 3358 1302.2 0 3358 296 3996 1302.2 0 3996 297 4477 1302.2 0 4477 298 2902 1302.2 0 2902 299 3247 1302.2 0 3247 300 9601 1302.2 0 9601 301 11129 1302.2 0 11129 Finished in 25.46 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 675,958 Reads with adapters: 4,533 (0.7%) Reads that were too short: 148 (0.0%) Reads written (passing filters): 4,385 (0.6%) Total basepairs processed: 118,349,953 bp Total written (filtered): 1,177,564 bp (1.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4533 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 2 0.0 3 0 0 1 1 16 44 0.0 3 0 27 2 15 17 4036 0.0 3 3917 95 13 11 18 27 0.0 3 2 14 7 4 19 51 0.0 3 1 1 25 24 20 12 0.0 3 1 0 0 11 21 3 0.0 3 0 0 0 3 23 2 0.0 3 0 0 0 2 24 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 1 27 3 0.0 3 0 0 1 2 28 2 0.0 3 0 0 0 2 31 3 0.0 3 0 0 0 3 32 1 0.0 3 0 0 0 1 34 3 0.0 3 0 0 0 3 35 3 0.0 3 0 0 0 3 36 4 0.0 3 0 0 0 4 37 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 41 6 0.0 3 0 0 0 6 42 1 0.0 3 0 0 0 1 43 3 0.0 3 0 0 0 3 44 9 0.0 3 0 0 0 9 45 3 0.0 3 0 0 0 3 47 3 0.0 3 0 0 0 3 49 2 0.0 3 0 0 0 2 50 4 0.0 3 0 0 0 4 51 2 0.0 3 0 0 0 2 52 2 0.0 3 0 0 0 2 53 3 0.0 3 0 0 0 3 54 5 0.0 3 0 0 0 5 55 3 0.0 3 0 0 0 3 56 2 0.0 3 0 0 0 2 57 6 0.0 3 0 0 0 6 58 3 0.0 3 0 0 0 3 59 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 63 2 0.0 3 0 0 0 2 64 2 0.0 3 0 0 0 2 65 3 0.0 3 0 0 0 3 66 2 0.0 3 0 0 0 2 67 11 0.0 3 0 0 0 11 68 4 0.0 3 0 0 0 4 69 3 0.0 3 0 0 0 3 70 2 0.0 3 0 0 0 2 71 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 75 7 0.0 3 0 0 0 7 76 2 0.0 3 0 0 0 2 77 5 0.0 3 0 0 0 5 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 3 0.0 3 0 0 0 3 83 2 0.0 3 0 0 0 2 84 5 0.0 3 0 0 0 5 85 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 87 3 0.0 3 0 0 0 3 88 2 0.0 3 0 0 0 2 89 4 0.0 3 0 0 0 4 90 2 0.0 3 0 0 0 2 91 5 0.0 3 0 0 0 5 92 5 0.0 3 0 0 0 5 93 3 0.0 3 0 0 0 3 94 3 0.0 3 0 0 0 3 95 2 0.0 3 0 0 0 2 96 3 0.0 3 0 0 0 3 97 2 0.0 3 0 0 0 2 99 4 0.0 3 0 0 0 4 100 1 0.0 3 0 0 0 1 101 3 0.0 3 0 0 0 3 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 6 0.0 3 0 0 0 6 107 3 0.0 3 0 0 0 3 108 6 0.0 3 0 0 0 6 109 9 0.0 3 0 0 1 8 111 2 0.0 3 0 0 0 2 112 18 0.0 3 0 0 1 17 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 10 0.0 3 0 0 0 10 116 5 0.0 3 0 0 0 5 117 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 4 0.0 3 0 0 0 4 132 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 4 0.0 3 0 0 1 3 143 4 0.0 3 0 0 0 4 148 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 157 2 0.0 3 0 0 0 2 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 179 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 211 2 0.0 3 0 0 0 2 213 2 0.0 3 0 0 0 2 214 16 0.0 3 0 0 0 16 218 3 0.0 3 0 0 0 3 219 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 283 7 0.0 3 0 0 0 7 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.20 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 4,385 Reads with adapters: 164 (3.7%) Reads written (passing filters): 4,385 (100.0%) Total basepairs processed: 1,177,564 bp Total written (filtered): 1,177,048 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 164 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.7% C: 20.7% G: 13.4% T: 23.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 145 68.5 0 145 4 15 17.1 0 15 5 3 4.3 0 3 6 1 1.1 0 1