This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 178.53 s (33 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 110,553 (2.1%) Reads that were too short: 48,315 (0.9%) Reads written (passing filters): 62,238 (1.2%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 10,709,680 bp (0.7%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 110553 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 24 1302.2 0 24 7 40 1302.2 0 40 8 174 1302.2 0 174 9 4213 1302.2 0 4213 10 12 1302.2 0 12 11 3 1302.2 0 3 12 2 1302.2 0 2 13 4 1302.2 0 4 14 1 1302.2 0 1 15 3 1302.2 0 3 16 5 1302.2 0 5 17 4 1302.2 0 4 18 9 1302.2 0 9 19 5 1302.2 0 5 20 9 1302.2 0 9 21 1 1302.2 0 1 22 5 1302.2 0 5 23 2 1302.2 0 2 24 4 1302.2 0 4 25 4 1302.2 0 4 26 2 1302.2 0 2 27 3 1302.2 0 3 28 5 1302.2 0 5 29 14 1302.2 0 14 30 21 1302.2 0 21 31 5 1302.2 0 5 32 21 1302.2 0 21 33 2 1302.2 0 2 34 5 1302.2 0 5 35 5 1302.2 0 5 36 8 1302.2 0 8 37 9 1302.2 0 9 38 8 1302.2 0 8 39 3 1302.2 0 3 40 3 1302.2 0 3 41 7 1302.2 0 7 42 2 1302.2 0 2 43 8 1302.2 0 8 44 8 1302.2 0 8 45 56 1302.2 0 56 46 79 1302.2 0 79 47 61 1302.2 0 61 48 8 1302.2 0 8 49 4 1302.2 0 4 50 7 1302.2 0 7 51 89 1302.2 0 89 52 80 1302.2 0 80 53 22 1302.2 0 22 54 2 1302.2 0 2 55 6 1302.2 0 6 56 6 1302.2 0 6 57 5 1302.2 0 5 58 4 1302.2 0 4 59 2 1302.2 0 2 60 26 1302.2 0 26 61 14 1302.2 0 14 62 2 1302.2 0 2 63 3 1302.2 0 3 64 2 1302.2 0 2 65 5 1302.2 0 5 66 8 1302.2 0 8 67 2 1302.2 0 2 68 6 1302.2 0 6 69 4 1302.2 0 4 70 8 1302.2 0 8 71 21 1302.2 0 21 72 1 1302.2 0 1 73 6 1302.2 0 6 74 1 1302.2 0 1 75 5 1302.2 0 5 76 65 1302.2 0 65 77 803 1302.2 0 803 78 314 1302.2 0 314 79 277 1302.2 0 277 80 213 1302.2 0 213 81 163 1302.2 0 163 82 98 1302.2 0 98 83 159 1302.2 0 159 84 260 1302.2 0 260 85 31 1302.2 0 31 86 30 1302.2 0 30 87 57 1302.2 0 57 88 76 1302.2 0 76 89 19 1302.2 0 19 90 175 1302.2 0 175 91 275 1302.2 0 275 92 896 1302.2 0 896 93 1987 1302.2 0 1987 94 1224 1302.2 0 1224 95 1082 1302.2 0 1082 96 234 1302.2 0 234 97 1154 1302.2 0 1154 98 663 1302.2 0 663 99 377 1302.2 0 377 100 949 1302.2 0 949 101 270 1302.2 0 270 102 48 1302.2 0 48 103 59 1302.2 0 59 104 190 1302.2 0 190 105 51 1302.2 0 51 106 63 1302.2 0 63 107 201 1302.2 0 201 108 41 1302.2 0 41 109 94 1302.2 0 94 110 244 1302.2 0 244 111 131 1302.2 0 131 112 142 1302.2 0 142 113 669 1302.2 0 669 114 1106 1302.2 0 1106 115 1928 1302.2 0 1928 116 1412 1302.2 0 1412 117 454 1302.2 0 454 118 653 1302.2 0 653 119 555 1302.2 0 555 120 306 1302.2 0 306 121 284 1302.2 0 284 122 328 1302.2 0 328 123 329 1302.2 0 329 124 388 1302.2 0 388 125 300 1302.2 0 300 126 183 1302.2 0 183 127 179 1302.2 0 179 128 382 1302.2 0 382 129 527 1302.2 0 527 130 662 1302.2 0 662 131 1084 1302.2 0 1084 132 464 1302.2 0 464 133 531 1302.2 0 531 134 541 1302.2 0 541 135 1652 1302.2 0 1652 136 1097 1302.2 0 1097 137 1252 1302.2 0 1252 138 511 1302.2 0 511 139 372 1302.2 0 372 140 220 1302.2 0 220 141 165 1302.2 0 165 142 310 1302.2 0 310 143 528 1302.2 0 528 144 520 1302.2 0 520 145 398 1302.2 0 398 146 205 1302.2 0 205 147 136 1302.2 0 136 148 133 1302.2 0 133 149 165 1302.2 0 165 150 220 1302.2 0 220 151 203 1302.2 0 203 152 290 1302.2 0 290 153 301 1302.2 0 301 154 493 1302.2 0 493 155 613 1302.2 0 613 156 359 1302.2 0 359 157 613 1302.2 0 613 158 478 1302.2 0 478 159 354 1302.2 0 354 160 315 1302.2 0 315 161 275 1302.2 0 275 162 439 1302.2 0 439 163 533 1302.2 0 533 164 760 1302.2 0 760 165 553 1302.2 0 553 166 441 1302.2 0 441 167 613 1302.2 0 613 168 561 1302.2 0 561 169 342 1302.2 0 342 170 364 1302.2 0 364 171 579 1302.2 0 579 172 255 1302.2 0 255 173 377 1302.2 0 377 174 309 1302.2 0 309 175 204 1302.2 0 204 176 178 1302.2 0 178 177 360 1302.2 0 360 178 723 1302.2 0 723 179 779 1302.2 0 779 180 724 1302.2 0 724 181 441 1302.2 0 441 182 515 1302.2 0 515 183 553 1302.2 0 553 184 563 1302.2 0 563 185 413 1302.2 0 413 186 163 1302.2 0 163 187 139 1302.2 0 139 188 266 1302.2 0 266 189 656 1302.2 0 656 190 1179 1302.2 0 1179 191 1107 1302.2 0 1107 192 585 1302.2 0 585 193 259 1302.2 0 259 194 186 1302.2 0 186 195 435 1302.2 0 435 196 417 1302.2 0 417 197 503 1302.2 0 503 198 345 1302.2 0 345 199 168 1302.2 0 168 200 198 1302.2 0 198 201 287 1302.2 0 287 202 924 1302.2 0 924 203 1850 1302.2 0 1850 204 1451 1302.2 0 1451 205 558 1302.2 0 558 206 259 1302.2 0 259 207 295 1302.2 0 295 208 362 1302.2 0 362 209 951 1302.2 0 951 210 1603 1302.2 0 1603 211 1123 1302.2 0 1123 212 833 1302.2 0 833 213 485 1302.2 0 485 214 460 1302.2 0 460 215 843 1302.2 0 843 216 777 1302.2 0 777 217 782 1302.2 0 782 218 411 1302.2 0 411 219 168 1302.2 0 168 220 285 1302.2 0 285 221 526 1302.2 0 526 222 368 1302.2 0 368 223 585 1302.2 0 585 224 688 1302.2 0 688 225 936 1302.2 0 936 226 1055 1302.2 0 1055 227 1251 1302.2 0 1251 228 434 1302.2 0 434 229 312 1302.2 0 312 230 200 1302.2 0 200 231 258 1302.2 0 258 232 512 1302.2 0 512 233 570 1302.2 0 570 234 894 1302.2 0 894 235 1238 1302.2 0 1238 236 973 1302.2 0 973 237 722 1302.2 0 722 238 246 1302.2 0 246 239 149 1302.2 0 149 240 187 1302.2 0 187 241 344 1302.2 0 344 242 472 1302.2 0 472 243 415 1302.2 0 415 244 453 1302.2 0 453 245 291 1302.2 0 291 246 199 1302.2 0 199 247 132 1302.2 0 132 248 125 1302.2 0 125 249 201 1302.2 0 201 250 447 1302.2 0 447 251 384 1302.2 0 384 252 969 1302.2 0 969 253 726 1302.2 0 726 254 505 1302.2 0 505 255 296 1302.2 0 296 256 131 1302.2 0 131 257 176 1302.2 0 176 258 133 1302.2 0 133 259 192 1302.2 0 192 260 401 1302.2 0 401 261 575 1302.2 0 575 262 470 1302.2 0 470 263 676 1302.2 0 676 264 501 1302.2 0 501 265 452 1302.2 0 452 266 212 1302.2 0 212 267 92 1302.2 0 92 268 108 1302.2 0 108 269 168 1302.2 0 168 270 435 1302.2 0 435 271 647 1302.2 0 647 272 484 1302.2 0 484 273 369 1302.2 0 369 274 274 1302.2 0 274 275 192 1302.2 0 192 276 226 1302.2 0 226 277 336 1302.2 0 336 278 370 1302.2 0 370 279 214 1302.2 0 214 280 132 1302.2 0 132 281 204 1302.2 0 204 282 203 1302.2 0 203 283 355 1302.2 0 355 284 435 1302.2 0 435 285 313 1302.2 0 313 286 195 1302.2 0 195 287 121 1302.2 0 121 288 161 1302.2 0 161 289 388 1302.2 0 388 290 658 1302.2 0 658 291 667 1302.2 0 667 292 373 1302.2 0 373 293 204 1302.2 0 204 294 170 1302.2 0 170 295 164 1302.2 0 164 296 362 1302.2 0 362 297 683 1302.2 0 683 298 716 1302.2 0 716 299 612 1302.2 0 612 300 647 1302.2 0 647 301 235 1302.2 0 235 Finished in 2.78 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 62,238 Reads with adapters: 4,185 (6.7%) Reads that were too short: 21 (0.0%) Reads written (passing filters): 4,164 (6.7%) Total basepairs processed: 10,709,680 bp Total written (filtered): 1,130,186 bp (10.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4185 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 37 0.0 3 0 34 2 1 20 4091 0.0 4 4024 58 6 0 3 21 13 0.0 4 3 8 1 1 22 4 0.0 4 0 0 1 0 3 23 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 1 47 8 0.0 4 0 0 0 0 8 53 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 69 5 0.0 4 0 0 0 1 4 81 1 0.0 4 0 0 0 0 1 99 2 0.0 4 0 0 0 0 2 102 1 0.0 4 0 0 0 0 1 103 2 0.0 4 0 0 0 0 2 110 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 121 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 143 2 0.0 4 0 0 0 0 2 157 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.18 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 4,164 Reads with adapters: 48 (1.2%) Reads written (passing filters): 4,164 (100.0%) Total basepairs processed: 1,130,186 bp Total written (filtered): 1,129,431 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 48 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 4.2% G: 89.6% T: 6.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 16.3 0 1 9 4 0.0 0 0 4 10 16 0.0 1 1 15 11 25 0.0 1 1 24 13 1 0.0 1 0 1 267 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.