This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.07 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 264,460 (9.0%) Reads that were too short: 120,575 (4.1%) Reads written (passing filters): 143,885 (4.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 25,003,948 bp (2.8%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 264460 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 623 714.6 0 623 7 11896 714.6 0 11896 8 32 714.6 0 32 9 23 714.6 0 23 10 34 714.6 0 34 11 28 714.6 0 28 12 22 714.6 0 22 13 29 714.6 0 29 14 29 714.6 0 29 15 20 714.6 0 20 16 30 714.6 0 30 17 29 714.6 0 29 18 22 714.6 0 22 19 31 714.6 0 31 20 36 714.6 0 36 21 19 714.6 0 19 22 27 714.6 0 27 23 27 714.6 0 27 24 26 714.6 0 26 25 27 714.6 0 27 26 15 714.6 0 15 27 27 714.6 0 27 28 33 714.6 0 33 29 23 714.6 0 23 30 15 714.6 0 15 31 28 714.6 0 28 32 23 714.6 0 23 33 18 714.6 0 18 34 20 714.6 0 20 35 26 714.6 0 26 36 24 714.6 0 24 37 31 714.6 0 31 38 28 714.6 0 28 39 20 714.6 0 20 40 22 714.6 0 22 41 24 714.6 0 24 42 27 714.6 0 27 43 24 714.6 0 24 44 14 714.6 0 14 45 27 714.6 0 27 46 25 714.6 0 25 47 19 714.6 0 19 48 20 714.6 0 20 49 17 714.6 0 17 50 19 714.6 0 19 51 26 714.6 0 26 52 22 714.6 0 22 53 20 714.6 0 20 54 25 714.6 0 25 55 18 714.6 0 18 56 15 714.6 0 15 57 29 714.6 0 29 58 142 714.6 0 142 59 46 714.6 0 46 60 50 714.6 0 50 61 23 714.6 0 23 62 17 714.6 0 17 63 23 714.6 0 23 64 16 714.6 0 16 65 24 714.6 0 24 66 13 714.6 0 13 67 15 714.6 0 15 68 31 714.6 0 31 69 18 714.6 0 18 70 22 714.6 0 22 71 31 714.6 0 31 72 36 714.6 0 36 73 262 714.6 0 262 74 187 714.6 0 187 75 489 714.6 0 489 76 428 714.6 0 428 77 352 714.6 0 352 78 445 714.6 0 445 79 607 714.6 0 607 80 448 714.6 0 448 81 557 714.6 0 557 82 428 714.6 0 428 83 500 714.6 0 500 84 370 714.6 0 370 85 460 714.6 0 460 86 647 714.6 0 647 87 333 714.6 0 333 88 863 714.6 0 863 89 411 714.6 0 411 90 533 714.6 0 533 91 603 714.6 0 603 92 676 714.6 0 676 93 553 714.6 0 553 94 1024 714.6 0 1024 95 1012 714.6 0 1012 96 1555 714.6 0 1555 97 1487 714.6 0 1487 98 1663 714.6 0 1663 99 1633 714.6 0 1633 100 885 714.6 0 885 101 772 714.6 0 772 102 692 714.6 0 692 103 757 714.6 0 757 104 1031 714.6 0 1031 105 1065 714.6 0 1065 106 2057 714.6 0 2057 107 1012 714.6 0 1012 108 1851 714.6 0 1851 109 1630 714.6 0 1630 110 1736 714.6 0 1736 111 848 714.6 0 848 112 1391 714.6 0 1391 113 1379 714.6 0 1379 114 2577 714.6 0 2577 115 2708 714.6 0 2708 116 1700 714.6 0 1700 117 1018 714.6 0 1018 118 641 714.6 0 641 119 434 714.6 0 434 120 425 714.6 0 425 121 709 714.6 0 709 122 1284 714.6 0 1284 123 1205 714.6 0 1205 124 848 714.6 0 848 125 521 714.6 0 521 126 787 714.6 0 787 127 941 714.6 0 941 128 865 714.6 0 865 129 1683 714.6 0 1683 130 2325 714.6 0 2325 131 1332 714.6 0 1332 132 1091 714.6 0 1091 133 799 714.6 0 799 134 675 714.6 0 675 135 1073 714.6 0 1073 136 1231 714.6 0 1231 137 1799 714.6 0 1799 138 1272 714.6 0 1272 139 723 714.6 0 723 140 802 714.6 0 802 141 1140 714.6 0 1140 142 1381 714.6 0 1381 143 948 714.6 0 948 144 836 714.6 0 836 145 740 714.6 0 740 146 889 714.6 0 889 147 934 714.6 0 934 148 1515 714.6 0 1515 149 1215 714.6 0 1215 150 1025 714.6 0 1025 151 1084 714.6 0 1084 152 695 714.6 0 695 153 958 714.6 0 958 154 1202 714.6 0 1202 155 1329 714.6 0 1329 156 1567 714.6 0 1567 157 1379 714.6 0 1379 158 1506 714.6 0 1506 159 950 714.6 0 950 160 681 714.6 0 681 161 1067 714.6 0 1067 162 846 714.6 0 846 163 1311 714.6 0 1311 164 1278 714.6 0 1278 165 1063 714.6 0 1063 166 1149 714.6 0 1149 167 1122 714.6 0 1122 168 1041 714.6 0 1041 169 920 714.6 0 920 170 750 714.6 0 750 171 714 714.6 0 714 172 590 714.6 0 590 173 563 714.6 0 563 174 677 714.6 0 677 175 930 714.6 0 930 176 1219 714.6 0 1219 177 894 714.6 0 894 178 907 714.6 0 907 179 870 714.6 0 870 180 792 714.6 0 792 181 894 714.6 0 894 182 932 714.6 0 932 183 968 714.6 0 968 184 926 714.6 0 926 185 1118 714.6 0 1118 186 1090 714.6 0 1090 187 1185 714.6 0 1185 188 1006 714.6 0 1006 189 750 714.6 0 750 190 634 714.6 0 634 191 714 714.6 0 714 192 1042 714.6 0 1042 193 1221 714.6 0 1221 194 1521 714.6 0 1521 195 933 714.6 0 933 196 1375 714.6 0 1375 197 615 714.6 0 615 198 794 714.6 0 794 199 1099 714.6 0 1099 200 1484 714.6 0 1484 201 1566 714.6 0 1566 202 1146 714.6 0 1146 203 873 714.6 0 873 204 999 714.6 0 999 205 1041 714.6 0 1041 206 966 714.6 0 966 207 991 714.6 0 991 208 1132 714.6 0 1132 209 1604 714.6 0 1604 210 1475 714.6 0 1475 211 1078 714.6 0 1078 212 744 714.6 0 744 213 757 714.6 0 757 214 799 714.6 0 799 215 889 714.6 0 889 216 879 714.6 0 879 217 896 714.6 0 896 218 923 714.6 0 923 219 1024 714.6 0 1024 220 880 714.6 0 880 221 1233 714.6 0 1233 222 1390 714.6 0 1390 223 967 714.6 0 967 224 849 714.6 0 849 225 887 714.6 0 887 226 872 714.6 0 872 227 883 714.6 0 883 228 1086 714.6 0 1086 229 1006 714.6 0 1006 230 1154 714.6 0 1154 231 919 714.6 0 919 232 908 714.6 0 908 233 809 714.6 0 809 234 875 714.6 0 875 235 1221 714.6 0 1221 236 1512 714.6 0 1512 237 1344 714.6 0 1344 238 1535 714.6 0 1535 239 933 714.6 0 933 240 989 714.6 0 989 241 1009 714.6 0 1009 242 1186 714.6 0 1186 243 1106 714.6 0 1106 244 1541 714.6 0 1541 245 1859 714.6 0 1859 246 1842 714.6 0 1842 247 1716 714.6 0 1716 248 1926 714.6 0 1926 249 1485 714.6 0 1485 250 582 714.6 0 582 251 563 714.6 0 563 252 297 714.6 0 297 253 739 714.6 0 739 254 1142 714.6 0 1142 255 1232 714.6 0 1232 256 1364 714.6 0 1364 257 2011 714.6 0 2011 258 2549 714.6 0 2549 259 2382 714.6 0 2382 260 1504 714.6 0 1504 261 862 714.6 0 862 262 568 714.6 0 568 263 735 714.6 0 735 264 824 714.6 0 824 265 899 714.6 0 899 266 826 714.6 0 826 267 1155 714.6 0 1155 268 1403 714.6 0 1403 269 1811 714.6 0 1811 270 1318 714.6 0 1318 271 1226 714.6 0 1226 272 935 714.6 0 935 273 1456 714.6 0 1456 274 1948 714.6 0 1948 275 1214 714.6 0 1214 276 1160 714.6 0 1160 277 700 714.6 0 700 278 626 714.6 0 626 279 884 714.6 0 884 280 1550 714.6 0 1550 281 1723 714.6 0 1723 282 1478 714.6 0 1478 283 1123 714.6 0 1123 284 885 714.6 0 885 285 1371 714.6 0 1371 286 1508 714.6 0 1508 287 1416 714.6 0 1416 288 1621 714.6 0 1621 289 894 714.6 0 894 290 877 714.6 0 877 291 908 714.6 0 908 292 1189 714.6 0 1189 293 1349 714.6 0 1349 294 1628 714.6 0 1628 295 2040 714.6 0 2040 296 2195 714.6 0 2195 297 1734 714.6 0 1734 298 1358 714.6 0 1358 299 1491 714.6 0 1491 300 1157 714.6 0 1157 301 2027 714.6 0 2027 Finished in 4.75 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 143,885 Reads with adapters: 12,484 (8.7%) Reads that were too short: 46 (0.0%) Reads written (passing filters): 12,438 (8.6%) Total basepairs processed: 25,003,948 bp Total written (filtered): 3,435,492 bp (13.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12484 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 93 0.0 3 0 87 4 2 17 9632 0.0 3 9303 286 34 9 18 58 0.0 3 3 48 4 3 19 2362 0.0 3 2288 57 12 5 20 257 0.0 3 242 12 2 1 21 2 0.0 3 0 1 1 22 2 0.0 3 0 0 0 2 23 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 42 3 0.0 3 0 0 0 3 43 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 72 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 1 97 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 108 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 136 3 0.0 3 0 0 0 3 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.44 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 12,438 Reads with adapters: 760 (6.1%) Reads written (passing filters): 12,438 (100.0%) Total basepairs processed: 3,435,492 bp Total written (filtered): 3,433,202 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 760 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 31.2% C: 43.4% G: 19.2% T: 6.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 751 194.3 0 751 4 8 48.6 0 8 5 1 12.1 0 1