This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.14 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 730,451 (25.0%) Reads that were too short: 154,766 (5.3%) Reads written (passing filters): 575,685 (19.7%) Total basepairs processed: 881,011,047 bp Total written (filtered): 96,701,752 bp (11.0%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 730451 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 290 714.6 0 290 7 12701 714.6 0 12701 8 10684 714.6 0 10684 9 279 714.6 0 279 10 92 714.6 0 92 11 51 714.6 0 51 12 66 714.6 0 66 13 62 714.6 0 62 14 74 714.6 0 74 15 88 714.6 0 88 16 60 714.6 0 60 17 54 714.6 0 54 18 43 714.6 0 43 19 52 714.6 0 52 20 52 714.6 0 52 21 48 714.6 0 48 22 43 714.6 0 43 23 46 714.6 0 46 24 42 714.6 0 42 25 47 714.6 0 47 26 85 714.6 0 85 27 77 714.6 0 77 28 89 714.6 0 89 29 60 714.6 0 60 30 67 714.6 0 67 31 44 714.6 0 44 32 52 714.6 0 52 33 37 714.6 0 37 34 49 714.6 0 49 35 60 714.6 0 60 36 78 714.6 0 78 37 49 714.6 0 49 38 58 714.6 0 58 39 56 714.6 0 56 40 86 714.6 0 86 41 44 714.6 0 44 42 56 714.6 0 56 43 69 714.6 0 69 44 53 714.6 0 53 45 63 714.6 0 63 46 55 714.6 0 55 47 33 714.6 0 33 48 65 714.6 0 65 49 57 714.6 0 57 50 64 714.6 0 64 51 64 714.6 0 64 52 52 714.6 0 52 53 59 714.6 0 59 54 92 714.6 0 92 55 56 714.6 0 56 56 47 714.6 0 47 57 57 714.6 0 57 58 65 714.6 0 65 59 82 714.6 0 82 60 58 714.6 0 58 61 69 714.6 0 69 62 46 714.6 0 46 63 77 714.6 0 77 64 45 714.6 0 45 65 52 714.6 0 52 66 81 714.6 0 81 67 66 714.6 0 66 68 117 714.6 0 117 69 210 714.6 0 210 70 119 714.6 0 119 71 117 714.6 0 117 72 230 714.6 0 230 73 86 714.6 0 86 74 83 714.6 0 83 75 93 714.6 0 93 76 91 714.6 0 91 77 162 714.6 0 162 78 780 714.6 0 780 79 2653 714.6 0 2653 80 1792 714.6 0 1792 81 4259 714.6 0 4259 82 4013 714.6 0 4013 83 3906 714.6 0 3906 84 6158 714.6 0 6158 85 5551 714.6 0 5551 86 3373 714.6 0 3373 87 966 714.6 0 966 88 1001 714.6 0 1001 89 682 714.6 0 682 90 1026 714.6 0 1026 91 704 714.6 0 704 92 1692 714.6 0 1692 93 2437 714.6 0 2437 94 1492 714.6 0 1492 95 3422 714.6 0 3422 96 2170 714.6 0 2170 97 1524 714.6 0 1524 98 4555 714.6 0 4555 99 2614 714.6 0 2614 100 1482 714.6 0 1482 101 2919 714.6 0 2919 102 2436 714.6 0 2436 103 1339 714.6 0 1339 104 1049 714.6 0 1049 105 1243 714.6 0 1243 106 1079 714.6 0 1079 107 999 714.6 0 999 108 1308 714.6 0 1308 109 1520 714.6 0 1520 110 2092 714.6 0 2092 111 2428 714.6 0 2428 112 1892 714.6 0 1892 113 2916 714.6 0 2916 114 3027 714.6 0 3027 115 3235 714.6 0 3235 116 3287 714.6 0 3287 117 4667 714.6 0 4667 118 13146 714.6 0 13146 119 41359 714.6 0 41359 120 18407 714.6 0 18407 121 4396 714.6 0 4396 122 5637 714.6 0 5637 123 5291 714.6 0 5291 124 2490 714.6 0 2490 125 2077 714.6 0 2077 126 2934 714.6 0 2934 127 2818 714.6 0 2818 128 2066 714.6 0 2066 129 3320 714.6 0 3320 130 2956 714.6 0 2956 131 2597 714.6 0 2597 132 3733 714.6 0 3733 133 2886 714.6 0 2886 134 3359 714.6 0 3359 135 3218 714.6 0 3218 136 3371 714.6 0 3371 137 3748 714.6 0 3748 138 3600 714.6 0 3600 139 4226 714.6 0 4226 140 15179 714.6 0 15179 141 11093 714.6 0 11093 142 10089 714.6 0 10089 143 4755 714.6 0 4755 144 4516 714.6 0 4516 145 11007 714.6 0 11007 146 39096 714.6 0 39096 147 24833 714.6 0 24833 148 8212 714.6 0 8212 149 34982 714.6 0 34982 150 5454 714.6 0 5454 151 3384 714.6 0 3384 152 4739 714.6 0 4739 153 4663 714.6 0 4663 154 2641 714.6 0 2641 155 3245 714.6 0 3245 156 2877 714.6 0 2877 157 2477 714.6 0 2477 158 2985 714.6 0 2985 159 2231 714.6 0 2231 160 2321 714.6 0 2321 161 1902 714.6 0 1902 162 2394 714.6 0 2394 163 2191 714.6 0 2191 164 2372 714.6 0 2372 165 4267 714.6 0 4267 166 7271 714.6 0 7271 167 7412 714.6 0 7412 168 2642 714.6 0 2642 169 2677 714.6 0 2677 170 2399 714.6 0 2399 171 2424 714.6 0 2424 172 2697 714.6 0 2697 173 2817 714.6 0 2817 174 2441 714.6 0 2441 175 2736 714.6 0 2736 176 2756 714.6 0 2756 177 1990 714.6 0 1990 178 2718 714.6 0 2718 179 2027 714.6 0 2027 180 1941 714.6 0 1941 181 2647 714.6 0 2647 182 2100 714.6 0 2100 183 2428 714.6 0 2428 184 2514 714.6 0 2514 185 2962 714.6 0 2962 186 2407 714.6 0 2407 187 2111 714.6 0 2111 188 2585 714.6 0 2585 189 2344 714.6 0 2344 190 2185 714.6 0 2185 191 2251 714.6 0 2251 192 2754 714.6 0 2754 193 3216 714.6 0 3216 194 2275 714.6 0 2275 195 3200 714.6 0 3200 196 3079 714.6 0 3079 197 2056 714.6 0 2056 198 2839 714.6 0 2839 199 1863 714.6 0 1863 200 2400 714.6 0 2400 201 2537 714.6 0 2537 202 1692 714.6 0 1692 203 1940 714.6 0 1940 204 2074 714.6 0 2074 205 2202 714.6 0 2202 206 2096 714.6 0 2096 207 1848 714.6 0 1848 208 1942 714.6 0 1942 209 2184 714.6 0 2184 210 1947 714.6 0 1947 211 1749 714.6 0 1749 212 1886 714.6 0 1886 213 1655 714.6 0 1655 214 1733 714.6 0 1733 215 1688 714.6 0 1688 216 2124 714.6 0 2124 217 2535 714.6 0 2535 218 2048 714.6 0 2048 219 1983 714.6 0 1983 220 1830 714.6 0 1830 221 1907 714.6 0 1907 222 1638 714.6 0 1638 223 1340 714.6 0 1340 224 1523 714.6 0 1523 225 1429 714.6 0 1429 226 1371 714.6 0 1371 227 1365 714.6 0 1365 228 1344 714.6 0 1344 229 1534 714.6 0 1534 230 1661 714.6 0 1661 231 1506 714.6 0 1506 232 1494 714.6 0 1494 233 1696 714.6 0 1696 234 1480 714.6 0 1480 235 1525 714.6 0 1525 236 1615 714.6 0 1615 237 2034 714.6 0 2034 238 1894 714.6 0 1894 239 1690 714.6 0 1690 240 1940 714.6 0 1940 241 1680 714.6 0 1680 242 1417 714.6 0 1417 243 1306 714.6 0 1306 244 1459 714.6 0 1459 245 1338 714.6 0 1338 246 1404 714.6 0 1404 247 1533 714.6 0 1533 248 1858 714.6 0 1858 249 1492 714.6 0 1492 250 1807 714.6 0 1807 251 1255 714.6 0 1255 252 974 714.6 0 974 253 1250 714.6 0 1250 254 1018 714.6 0 1018 255 1030 714.6 0 1030 256 1041 714.6 0 1041 257 1116 714.6 0 1116 258 1131 714.6 0 1131 259 1167 714.6 0 1167 260 1667 714.6 0 1667 261 1451 714.6 0 1451 262 1230 714.6 0 1230 263 1223 714.6 0 1223 264 975 714.6 0 975 265 1059 714.6 0 1059 266 1303 714.6 0 1303 267 1129 714.6 0 1129 268 1343 714.6 0 1343 269 1449 714.6 0 1449 270 1544 714.6 0 1544 271 1525 714.6 0 1525 272 1103 714.6 0 1103 273 1324 714.6 0 1324 274 1254 714.6 0 1254 275 1217 714.6 0 1217 276 1136 714.6 0 1136 277 997 714.6 0 997 278 936 714.6 0 936 279 1211 714.6 0 1211 280 761 714.6 0 761 281 1182 714.6 0 1182 282 1115 714.6 0 1115 283 765 714.6 0 765 284 1122 714.6 0 1122 285 1089 714.6 0 1089 286 1029 714.6 0 1029 287 1177 714.6 0 1177 288 1704 714.6 0 1704 289 1725 714.6 0 1725 290 1763 714.6 0 1763 291 1786 714.6 0 1786 292 1812 714.6 0 1812 293 1590 714.6 0 1590 294 2112 714.6 0 2112 295 2335 714.6 0 2335 296 2005 714.6 0 2005 297 2546 714.6 0 2546 298 2420 714.6 0 2420 299 1760 714.6 0 1760 300 1317 714.6 0 1317 301 2157 714.6 0 2157 Finished in 19.82 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 575,685 Reads with adapters: 11,173 (1.9%) Reads that were too short: 150 (0.0%) Reads written (passing filters): 11,023 (1.9%) Total basepairs processed: 96,701,752 bp Total written (filtered): 2,996,073 bp (3.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11173 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 6 0.0 3 0 0 0 0 6 18 6 0.0 3 0 0 5 1 19 91 0.0 3 0 86 2 0 3 20 10763 0.0 4 10625 130 3 4 1 21 36 0.0 4 6 28 1 1 22 8 0.0 4 1 0 2 1 4 23 7 0.0 4 0 0 0 4 3 24 2 0.0 4 0 0 0 0 2 25 1 0.0 4 0 0 0 0 1 26 3 0.0 4 0 0 0 0 3 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 1 36 3 0.0 4 0 0 0 0 3 37 1 0.0 4 0 0 0 0 1 39 3 0.0 4 0 0 0 1 2 40 1 0.0 4 0 0 0 0 1 41 2 0.0 4 0 0 0 0 2 42 3 0.0 4 0 0 0 1 2 43 1 0.0 4 0 0 0 0 1 45 5 0.0 4 0 0 0 1 4 47 1 0.0 4 0 0 0 0 1 48 4 0.0 4 0 0 0 0 4 50 39 0.0 4 0 0 0 0 39 52 1 0.0 4 0 0 0 0 1 53 3 0.0 4 0 0 0 0 3 54 1 0.0 4 0 0 0 0 1 55 2 0.0 4 0 0 0 0 2 56 6 0.0 4 0 0 0 0 6 58 2 0.0 4 0 0 0 0 2 59 2 0.0 4 0 0 0 0 2 61 2 0.0 4 0 0 0 0 2 62 1 0.0 4 0 0 0 0 1 66 3 0.0 4 0 0 0 0 3 67 1 0.0 4 0 0 0 0 1 68 3 0.0 4 0 0 0 0 3 69 2 0.0 4 0 0 0 0 2 70 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 75 2 0.0 4 0 0 0 0 2 76 4 0.0 4 0 0 0 1 3 77 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 79 6 0.0 4 0 0 0 0 6 82 2 0.0 4 0 0 0 0 2 83 1 0.0 4 0 0 0 0 1 84 4 0.0 4 0 0 0 0 4 85 2 0.0 4 0 0 0 0 2 86 3 0.0 4 0 0 0 0 3 87 1 0.0 4 0 0 0 0 1 88 3 0.0 4 0 0 0 0 3 89 2 0.0 4 0 0 0 0 2 90 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 94 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 96 3 0.0 4 0 0 0 0 3 97 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 99 2 0.0 4 0 0 0 0 2 100 2 0.0 4 0 0 0 0 2 101 2 0.0 4 0 0 0 0 2 103 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 2 0.0 4 0 0 0 0 2 110 2 0.0 4 0 0 0 0 2 111 4 0.0 4 0 0 0 0 4 112 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 114 2 0.0 4 0 0 0 0 2 115 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 118 2 0.0 4 0 0 0 0 2 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 121 2 0.0 4 0 0 0 0 2 122 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 124 4 0.0 4 0 0 0 0 4 125 2 0.0 4 0 0 0 0 2 126 2 0.0 4 0 0 0 0 2 127 4 0.0 4 0 0 0 0 4 129 1 0.0 4 0 0 0 0 1 131 4 0.0 4 0 0 0 0 4 132 2 0.0 4 0 0 0 0 2 133 2 0.0 4 0 0 0 0 2 135 2 0.0 4 0 0 0 0 2 137 2 0.0 4 0 0 0 0 2 141 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 146 2 0.0 4 0 0 0 0 2 148 2 0.0 4 0 0 0 0 2 150 2 0.0 4 0 0 0 0 2 151 1 0.0 4 0 0 0 0 1 152 3 0.0 4 0 0 0 0 3 153 1 0.0 4 0 0 0 0 1 155 2 0.0 4 0 0 0 0 2 156 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 0 0 0 1 159 4 0.0 4 0 0 0 0 4 160 2 0.0 4 0 0 0 0 2 161 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 166 2 0.0 4 0 0 0 0 2 180 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 208 3 0.0 4 0 0 0 0 3 216 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 244 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 11,023 Reads with adapters: 576 (5.2%) Reads written (passing filters): 11,023 (100.0%) Total basepairs processed: 2,996,073 bp Total written (filtered): 2,989,783 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 576 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.5% C: 0.9% G: 98.4% T: 0.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 172.2 0 1 4 1 43.1 0 1 5 1 10.8 0 1 9 3 0.0 0 0 3 10 22 0.0 1 4 18 11 547 0.0 1 2 545 14 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.