This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.08 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 21,556 (0.7%) Reads that were too short: 5,519 (0.2%) Reads written (passing filters): 16,037 (0.5%) Total basepairs processed: 881,011,047 bp Total written (filtered): 3,595,285 bp (0.4%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 21556 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 80 714.6 0 80 7 70 714.6 0 70 8 116 714.6 0 116 9 1904 714.6 0 1904 10 82 714.6 0 82 11 63 714.6 0 63 12 57 714.6 0 57 13 73 714.6 0 73 14 56 714.6 0 56 15 48 714.6 0 48 16 62 714.6 0 62 17 73 714.6 0 73 18 71 714.6 0 71 19 64 714.6 0 64 20 56 714.6 0 56 21 292 714.6 0 292 22 66 714.6 0 66 23 60 714.6 0 60 24 67 714.6 0 67 25 56 714.6 0 56 26 47 714.6 0 47 27 50 714.6 0 50 28 65 714.6 0 65 29 42 714.6 0 42 30 65 714.6 0 65 31 52 714.6 0 52 32 48 714.6 0 48 33 39 714.6 0 39 34 65 714.6 0 65 35 43 714.6 0 43 36 56 714.6 0 56 37 72 714.6 0 72 38 57 714.6 0 57 39 59 714.6 0 59 40 92 714.6 0 92 41 67 714.6 0 67 42 81 714.6 0 81 43 265 714.6 0 265 44 55 714.6 0 55 45 67 714.6 0 67 46 503 714.6 0 503 47 411 714.6 0 411 48 209 714.6 0 209 49 1112 714.6 0 1112 50 885 714.6 0 885 51 82 714.6 0 82 52 62 714.6 0 62 53 42 714.6 0 42 54 56 714.6 0 56 55 55 714.6 0 55 56 55 714.6 0 55 57 44 714.6 0 44 58 49 714.6 0 49 59 51 714.6 0 51 60 79 714.6 0 79 61 67 714.6 0 67 62 43 714.6 0 43 63 39 714.6 0 39 64 57 714.6 0 57 65 42 714.6 0 42 66 41 714.6 0 41 67 50 714.6 0 50 68 49 714.6 0 49 69 60 714.6 0 60 70 50 714.6 0 50 71 50 714.6 0 50 72 55 714.6 0 55 73 58 714.6 0 58 74 36 714.6 0 36 75 37 714.6 0 37 76 48 714.6 0 48 77 68 714.6 0 68 78 99 714.6 0 99 79 87 714.6 0 87 80 52 714.6 0 52 81 40 714.6 0 40 82 47 714.6 0 47 83 55 714.6 0 55 84 103 714.6 0 103 85 178 714.6 0 178 86 119 714.6 0 119 87 35 714.6 0 35 88 36 714.6 0 36 89 42 714.6 0 42 90 30 714.6 0 30 91 48 714.6 0 48 92 40 714.6 0 40 93 41 714.6 0 41 94 41 714.6 0 41 95 50 714.6 0 50 96 39 714.6 0 39 97 31 714.6 0 31 98 25 714.6 0 25 99 40 714.6 0 40 100 38 714.6 0 38 101 33 714.6 0 33 102 34 714.6 0 34 103 22 714.6 0 22 104 32 714.6 0 32 105 23 714.6 0 23 106 33 714.6 0 33 107 33 714.6 0 33 108 28 714.6 0 28 109 41 714.6 0 41 110 26 714.6 0 26 111 33 714.6 0 33 112 138 714.6 0 138 113 146 714.6 0 146 114 143 714.6 0 143 115 31 714.6 0 31 116 32 714.6 0 32 117 41 714.6 0 41 118 36 714.6 0 36 119 33 714.6 0 33 120 27 714.6 0 27 121 27 714.6 0 27 122 25 714.6 0 25 123 40 714.6 0 40 124 31 714.6 0 31 125 44 714.6 0 44 126 37 714.6 0 37 127 60 714.6 0 60 128 59 714.6 0 59 129 48 714.6 0 48 130 78 714.6 0 78 131 51 714.6 0 51 132 64 714.6 0 64 133 119 714.6 0 119 134 126 714.6 0 126 135 57 714.6 0 57 136 107 714.6 0 107 137 49 714.6 0 49 138 36 714.6 0 36 139 49 714.6 0 49 140 50 714.6 0 50 141 106 714.6 0 106 142 827 714.6 0 827 143 129 714.6 0 129 144 46 714.6 0 46 145 49 714.6 0 49 146 29 714.6 0 29 147 66 714.6 0 66 148 52 714.6 0 52 149 49 714.6 0 49 150 23 714.6 0 23 151 37 714.6 0 37 152 73 714.6 0 73 153 98 714.6 0 98 154 98 714.6 0 98 155 37 714.6 0 37 156 35 714.6 0 35 157 37 714.6 0 37 158 36 714.6 0 36 159 44 714.6 0 44 160 19 714.6 0 19 161 30 714.6 0 30 162 32 714.6 0 32 163 33 714.6 0 33 164 36 714.6 0 36 165 34 714.6 0 34 166 33 714.6 0 33 167 24 714.6 0 24 168 35 714.6 0 35 169 42 714.6 0 42 170 25 714.6 0 25 171 31 714.6 0 31 172 38 714.6 0 38 173 36 714.6 0 36 174 29 714.6 0 29 175 39 714.6 0 39 176 43 714.6 0 43 177 33 714.6 0 33 178 29 714.6 0 29 179 30 714.6 0 30 180 38 714.6 0 38 181 26 714.6 0 26 182 40 714.6 0 40 183 32 714.6 0 32 184 45 714.6 0 45 185 36 714.6 0 36 186 19 714.6 0 19 187 33 714.6 0 33 188 31 714.6 0 31 189 37 714.6 0 37 190 40 714.6 0 40 191 42 714.6 0 42 192 32 714.6 0 32 193 28 714.6 0 28 194 28 714.6 0 28 195 30 714.6 0 30 196 39 714.6 0 39 197 31 714.6 0 31 198 30 714.6 0 30 199 26 714.6 0 26 200 33 714.6 0 33 201 33 714.6 0 33 202 25 714.6 0 25 203 27 714.6 0 27 204 38 714.6 0 38 205 41 714.6 0 41 206 23 714.6 0 23 207 42 714.6 0 42 208 26 714.6 0 26 209 32 714.6 0 32 210 22 714.6 0 22 211 26 714.6 0 26 212 59 714.6 0 59 213 56 714.6 0 56 214 60 714.6 0 60 215 62 714.6 0 62 216 53 714.6 0 53 217 46 714.6 0 46 218 58 714.6 0 58 219 52 714.6 0 52 220 49 714.6 0 49 221 57 714.6 0 57 222 45 714.6 0 45 223 32 714.6 0 32 224 208 714.6 0 208 225 39 714.6 0 39 226 32 714.6 0 32 227 33 714.6 0 33 228 25 714.6 0 25 229 27 714.6 0 27 230 60 714.6 0 60 231 51 714.6 0 51 232 31 714.6 0 31 233 31 714.6 0 31 234 28 714.6 0 28 235 103 714.6 0 103 236 135 714.6 0 135 237 110 714.6 0 110 238 66 714.6 0 66 239 61 714.6 0 61 240 37 714.6 0 37 241 28 714.6 0 28 242 30 714.6 0 30 243 40 714.6 0 40 244 58 714.6 0 58 245 65 714.6 0 65 246 63 714.6 0 63 247 53 714.6 0 53 248 40 714.6 0 40 249 44 714.6 0 44 250 34 714.6 0 34 251 29 714.6 0 29 252 45 714.6 0 45 253 78 714.6 0 78 254 69 714.6 0 69 255 70 714.6 0 70 256 120 714.6 0 120 257 154 714.6 0 154 258 61 714.6 0 61 259 35 714.6 0 35 260 37 714.6 0 37 261 54 714.6 0 54 262 29 714.6 0 29 263 25 714.6 0 25 264 38 714.6 0 38 265 16 714.6 0 16 266 35 714.6 0 35 267 33 714.6 0 33 268 22 714.6 0 22 269 21 714.6 0 21 270 26 714.6 0 26 271 28 714.6 0 28 272 26 714.6 0 26 273 31 714.6 0 31 274 42 714.6 0 42 275 23 714.6 0 23 276 31 714.6 0 31 277 24 714.6 0 24 278 32 714.6 0 32 279 32 714.6 0 32 280 34 714.6 0 34 281 56 714.6 0 56 282 36 714.6 0 36 283 35 714.6 0 35 284 65 714.6 0 65 285 129 714.6 0 129 286 57 714.6 0 57 287 80 714.6 0 80 288 33 714.6 0 33 289 38 714.6 0 38 290 46 714.6 0 46 291 101 714.6 0 101 292 76 714.6 0 76 293 49 714.6 0 49 294 22 714.6 0 22 295 18 714.6 0 18 296 22 714.6 0 22 297 29 714.6 0 29 298 106 714.6 0 106 299 364 714.6 0 364 300 359 714.6 0 359 301 35 714.6 0 35 Finished in 0.64 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 16,037 Reads with adapters: 1,973 (12.3%) Reads that were too short: 28 (0.2%) Reads written (passing filters): 1,945 (12.1%) Total basepairs processed: 3,595,285 bp Total written (filtered): 528,738 bp (14.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1973 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 1 0.0 3 0 0 0 1 16 15 0.0 3 0 11 3 1 17 1828 0.0 3 1749 70 6 3 18 17 0.0 3 0 1 14 2 19 32 0.0 3 1 0 1 30 20 3 0.0 3 0 0 0 3 21 2 0.0 3 1 1 25 2 0.0 3 0 0 0 2 26 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 1 38 1 0.0 3 0 0 0 1 39 4 0.0 3 0 0 0 4 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 50 11 0.0 3 0 0 0 11 53 3 0.0 3 0 0 0 3 54 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 101 5 0.0 3 0 0 0 5 102 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 149 2 0.0 3 0 0 0 2 151 3 0.0 3 0 0 0 3 152 2 0.0 3 0 0 0 2 153 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 170 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 179 4 0.0 3 0 0 0 4 199 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (31 us/read; 1.94 M reads/minute). === Summary === Total reads processed: 1,945 Reads with adapters: 97 (5.0%) Reads written (passing filters): 1,945 (100.0%) Total basepairs processed: 528,738 bp Total written (filtered): 528,446 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 97 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 55.7% C: 13.4% G: 23.7% T: 7.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 96 30.4 0 96 4 1 7.6 0 1