This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.25 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 61,248 (2.1%) Reads that were too short: 9,063 (0.3%) Reads written (passing filters): 52,185 (1.8%) Total basepairs processed: 881,011,047 bp Total written (filtered): 8,768,207 bp (1.0%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 61248 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 117 714.6 0 117 7 1844 714.6 0 1844 8 172 714.6 0 172 9 4076 714.6 0 4076 10 29 714.6 0 29 11 31 714.6 0 31 12 40 714.6 0 40 13 27 714.6 0 27 14 35 714.6 0 35 15 27 714.6 0 27 16 34 714.6 0 34 17 46 714.6 0 46 18 43 714.6 0 43 19 36 714.6 0 36 20 33 714.6 0 33 21 37 714.6 0 37 22 34 714.6 0 34 23 36 714.6 0 36 24 34 714.6 0 34 25 32 714.6 0 32 26 28 714.6 0 28 27 36 714.6 0 36 28 29 714.6 0 29 29 53 714.6 0 53 30 2943 714.6 0 2943 31 367 714.6 0 367 32 41 714.6 0 41 33 142 714.6 0 142 34 103 714.6 0 103 35 100 714.6 0 100 36 44 714.6 0 44 37 33 714.6 0 33 38 31 714.6 0 31 39 30 714.6 0 30 40 26 714.6 0 26 41 35 714.6 0 35 42 27 714.6 0 27 43 36 714.6 0 36 44 31 714.6 0 31 45 28 714.6 0 28 46 53 714.6 0 53 47 40 714.6 0 40 48 21 714.6 0 21 49 31 714.6 0 31 50 53 714.6 0 53 51 23 714.6 0 23 52 31 714.6 0 31 53 41 714.6 0 41 54 32 714.6 0 32 55 195 714.6 0 195 56 40 714.6 0 40 57 46 714.6 0 46 58 106 714.6 0 106 59 33 714.6 0 33 60 59 714.6 0 59 61 28 714.6 0 28 62 25 714.6 0 25 63 29 714.6 0 29 64 32 714.6 0 32 65 30 714.6 0 30 66 30 714.6 0 30 67 15 714.6 0 15 68 33 714.6 0 33 69 26 714.6 0 26 70 21 714.6 0 21 71 28 714.6 0 28 72 156 714.6 0 156 73 29 714.6 0 29 74 29 714.6 0 29 75 21 714.6 0 21 76 24 714.6 0 24 77 31 714.6 0 31 78 28 714.6 0 28 79 30 714.6 0 30 80 22 714.6 0 22 81 19 714.6 0 19 82 33 714.6 0 33 83 31 714.6 0 31 84 90 714.6 0 90 85 37 714.6 0 37 86 26 714.6 0 26 87 21 714.6 0 21 88 31 714.6 0 31 89 28 714.6 0 28 90 34 714.6 0 34 91 36 714.6 0 36 92 45 714.6 0 45 93 53 714.6 0 53 94 80 714.6 0 80 95 44 714.6 0 44 96 68 714.6 0 68 97 86 714.6 0 86 98 51 714.6 0 51 99 55 714.6 0 55 100 58 714.6 0 58 101 50 714.6 0 50 102 39 714.6 0 39 103 36 714.6 0 36 104 54 714.6 0 54 105 57 714.6 0 57 106 445 714.6 0 445 107 1413 714.6 0 1413 108 1663 714.6 0 1663 109 1060 714.6 0 1060 110 26 714.6 0 26 111 31 714.6 0 31 112 35 714.6 0 35 113 25 714.6 0 25 114 26 714.6 0 26 115 28 714.6 0 28 116 45 714.6 0 45 117 42 714.6 0 42 118 20 714.6 0 20 119 34 714.6 0 34 120 36 714.6 0 36 121 82 714.6 0 82 122 54 714.6 0 54 123 53 714.6 0 53 124 41 714.6 0 41 125 35 714.6 0 35 126 29 714.6 0 29 127 132 714.6 0 132 128 395 714.6 0 395 129 415 714.6 0 415 130 87 714.6 0 87 131 78 714.6 0 78 132 38 714.6 0 38 133 42 714.6 0 42 134 66 714.6 0 66 135 86 714.6 0 86 136 237 714.6 0 237 137 165 714.6 0 165 138 116 714.6 0 116 139 37 714.6 0 37 140 75 714.6 0 75 141 105 714.6 0 105 142 121 714.6 0 121 143 273 714.6 0 273 144 681 714.6 0 681 145 389 714.6 0 389 146 60 714.6 0 60 147 106 714.6 0 106 148 40 714.6 0 40 149 97 714.6 0 97 150 53 714.6 0 53 151 44 714.6 0 44 152 60 714.6 0 60 153 339 714.6 0 339 154 157 714.6 0 157 155 130 714.6 0 130 156 83 714.6 0 83 157 40 714.6 0 40 158 34 714.6 0 34 159 84 714.6 0 84 160 64 714.6 0 64 161 39 714.6 0 39 162 41 714.6 0 41 163 32 714.6 0 32 164 45 714.6 0 45 165 76 714.6 0 76 166 68 714.6 0 68 167 81 714.6 0 81 168 68 714.6 0 68 169 158 714.6 0 158 170 146 714.6 0 146 171 68 714.6 0 68 172 36 714.6 0 36 173 51 714.6 0 51 174 95 714.6 0 95 175 132 714.6 0 132 176 87 714.6 0 87 177 46 714.6 0 46 178 51 714.6 0 51 179 91 714.6 0 91 180 77 714.6 0 77 181 120 714.6 0 120 182 161 714.6 0 161 183 277 714.6 0 277 184 318 714.6 0 318 185 902 714.6 0 902 186 20754 714.6 0 20754 187 2532 714.6 0 2532 188 1404 714.6 0 1404 189 47 714.6 0 47 190 28 714.6 0 28 191 53 714.6 0 53 192 48 714.6 0 48 193 32 714.6 0 32 194 53 714.6 0 53 195 39 714.6 0 39 196 22 714.6 0 22 197 39 714.6 0 39 198 32 714.6 0 32 199 34 714.6 0 34 200 58 714.6 0 58 201 33 714.6 0 33 202 56 714.6 0 56 203 78 714.6 0 78 204 191 714.6 0 191 205 198 714.6 0 198 206 72 714.6 0 72 207 51 714.6 0 51 208 55 714.6 0 55 209 77 714.6 0 77 210 66 714.6 0 66 211 42 714.6 0 42 212 50 714.6 0 50 213 62 714.6 0 62 214 58 714.6 0 58 215 63 714.6 0 63 216 111 714.6 0 111 217 688 714.6 0 688 218 1375 714.6 0 1375 219 373 714.6 0 373 220 31 714.6 0 31 221 43 714.6 0 43 222 60 714.6 0 60 223 42 714.6 0 42 224 30 714.6 0 30 225 34 714.6 0 34 226 69 714.6 0 69 227 130 714.6 0 130 228 39 714.6 0 39 229 47 714.6 0 47 230 45 714.6 0 45 231 37 714.6 0 37 232 38 714.6 0 38 233 51 714.6 0 51 234 45 714.6 0 45 235 59 714.6 0 59 236 74 714.6 0 74 237 76 714.6 0 76 238 86 714.6 0 86 239 52 714.6 0 52 240 35 714.6 0 35 241 38 714.6 0 38 242 35 714.6 0 35 243 50 714.6 0 50 244 65 714.6 0 65 245 147 714.6 0 147 246 62 714.6 0 62 247 36 714.6 0 36 248 33 714.6 0 33 249 22 714.6 0 22 250 30 714.6 0 30 251 35 714.6 0 35 252 76 714.6 0 76 253 140 714.6 0 140 254 131 714.6 0 131 255 88 714.6 0 88 256 64 714.6 0 64 257 69 714.6 0 69 258 68 714.6 0 68 259 48 714.6 0 48 260 56 714.6 0 56 261 71 714.6 0 71 262 128 714.6 0 128 263 116 714.6 0 116 264 61 714.6 0 61 265 70 714.6 0 70 266 61 714.6 0 61 267 38 714.6 0 38 268 41 714.6 0 41 269 49 714.6 0 49 270 39 714.6 0 39 271 44 714.6 0 44 272 67 714.6 0 67 273 65 714.6 0 65 274 47 714.6 0 47 275 68 714.6 0 68 276 44 714.6 0 44 277 93 714.6 0 93 278 199 714.6 0 199 279 116 714.6 0 116 280 185 714.6 0 185 281 88 714.6 0 88 282 99 714.6 0 99 283 49 714.6 0 49 284 46 714.6 0 46 285 170 714.6 0 170 286 120 714.6 0 120 287 55 714.6 0 55 288 33 714.6 0 33 289 46 714.6 0 46 290 44 714.6 0 44 291 41 714.6 0 41 292 47 714.6 0 47 293 38 714.6 0 38 294 39 714.6 0 39 295 31 714.6 0 31 296 37 714.6 0 37 297 82 714.6 0 82 298 111 714.6 0 111 299 89 714.6 0 89 300 76 714.6 0 76 301 38 714.6 0 38 Finished in 1.91 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 52,185 Reads with adapters: 1,905 (3.7%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 1,900 (3.6%) Total basepairs processed: 8,768,207 bp Total written (filtered): 513,596 bp (5.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1905 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 2 0.0 3 0 0 2 19 24 0.0 3 0 23 0 0 1 20 1802 0.0 4 1783 17 0 0 2 21 5 0.0 4 1 4 23 1 0.0 4 1 25 2 0.0 4 0 0 0 0 2 70 1 0.0 4 1 107 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 59 0.0 4 0 0 0 0 59 149 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.06 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 1,900 Reads with adapters: 42 (2.2%) Reads written (passing filters): 1,900 (100.0%) Total basepairs processed: 513,596 bp Total written (filtered): 512,627 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 42 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.5% C: 2.4% G: 88.1% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 29.7 0 2 11 37 0.0 1 0 37 12 1 0.0 1 0 1 272 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.