This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.08 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 69,969 (2.4%) Reads that were too short: 33,357 (1.1%) Reads written (passing filters): 36,612 (1.3%) Total basepairs processed: 881,011,047 bp Total written (filtered): 7,373,023 bp (0.8%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 69969 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 324 714.6 0 324 7 9539 714.6 0 9539 8 63 714.6 0 63 9 20 714.6 0 20 10 40 714.6 0 40 11 27 714.6 0 27 12 280 714.6 0 280 13 188 714.6 0 188 14 28 714.6 0 28 15 24 714.6 0 24 16 27 714.6 0 27 17 29 714.6 0 29 18 25 714.6 0 25 19 22 714.6 0 22 20 28 714.6 0 28 21 24 714.6 0 24 22 24 714.6 0 24 23 26 714.6 0 26 24 21 714.6 0 21 25 27 714.6 0 27 26 31 714.6 0 31 27 23 714.6 0 23 28 28 714.6 0 28 29 29 714.6 0 29 30 39 714.6 0 39 31 25 714.6 0 25 32 30 714.6 0 30 33 34 714.6 0 34 34 25 714.6 0 25 35 20 714.6 0 20 36 22 714.6 0 22 37 40 714.6 0 40 38 27 714.6 0 27 39 24 714.6 0 24 40 23 714.6 0 23 41 31 714.6 0 31 42 33 714.6 0 33 43 18 714.6 0 18 44 20 714.6 0 20 45 28 714.6 0 28 46 19 714.6 0 19 47 17 714.6 0 17 48 22 714.6 0 22 49 32 714.6 0 32 50 30 714.6 0 30 51 11 714.6 0 11 52 22 714.6 0 22 53 20 714.6 0 20 54 28 714.6 0 28 55 37 714.6 0 37 56 26 714.6 0 26 57 17 714.6 0 17 58 15 714.6 0 15 59 14 714.6 0 14 60 22 714.6 0 22 61 23 714.6 0 23 62 38 714.6 0 38 63 22 714.6 0 22 64 23 714.6 0 23 65 32 714.6 0 32 66 24 714.6 0 24 67 31 714.6 0 31 68 17 714.6 0 17 69 31 714.6 0 31 70 31 714.6 0 31 71 28 714.6 0 28 72 39 714.6 0 39 73 35 714.6 0 35 74 70 714.6 0 70 75 165 714.6 0 165 76 106 714.6 0 106 77 88 714.6 0 88 78 81 714.6 0 81 79 168 714.6 0 168 80 345 714.6 0 345 81 290 714.6 0 290 82 2028 714.6 0 2028 83 905 714.6 0 905 84 407 714.6 0 407 85 149 714.6 0 149 86 198 714.6 0 198 87 92 714.6 0 92 88 160 714.6 0 160 89 209 714.6 0 209 90 145 714.6 0 145 91 67 714.6 0 67 92 133 714.6 0 133 93 146 714.6 0 146 94 88 714.6 0 88 95 92 714.6 0 92 96 64 714.6 0 64 97 78 714.6 0 78 98 56 714.6 0 56 99 46 714.6 0 46 100 49 714.6 0 49 101 72 714.6 0 72 102 70 714.6 0 70 103 37 714.6 0 37 104 22 714.6 0 22 105 43 714.6 0 43 106 50 714.6 0 50 107 43 714.6 0 43 108 39 714.6 0 39 109 56 714.6 0 56 110 56 714.6 0 56 111 71 714.6 0 71 112 72 714.6 0 72 113 116 714.6 0 116 114 116 714.6 0 116 115 74 714.6 0 74 116 70 714.6 0 70 117 115 714.6 0 115 118 140 714.6 0 140 119 167 714.6 0 167 120 121 714.6 0 121 121 118 714.6 0 118 122 185 714.6 0 185 123 182 714.6 0 182 124 130 714.6 0 130 125 203 714.6 0 203 126 148 714.6 0 148 127 168 714.6 0 168 128 96 714.6 0 96 129 96 714.6 0 96 130 94 714.6 0 94 131 126 714.6 0 126 132 161 714.6 0 161 133 184 714.6 0 184 134 117 714.6 0 117 135 138 714.6 0 138 136 138 714.6 0 138 137 130 714.6 0 130 138 154 714.6 0 154 139 174 714.6 0 174 140 102 714.6 0 102 141 76 714.6 0 76 142 116 714.6 0 116 143 169 714.6 0 169 144 147 714.6 0 147 145 118 714.6 0 118 146 112 714.6 0 112 147 119 714.6 0 119 148 104 714.6 0 104 149 133 714.6 0 133 150 106 714.6 0 106 151 118 714.6 0 118 152 163 714.6 0 163 153 217 714.6 0 217 154 188 714.6 0 188 155 130 714.6 0 130 156 188 714.6 0 188 157 161 714.6 0 161 158 156 714.6 0 156 159 164 714.6 0 164 160 108 714.6 0 108 161 88 714.6 0 88 162 96 714.6 0 96 163 200 714.6 0 200 164 175 714.6 0 175 165 166 714.6 0 166 166 135 714.6 0 135 167 730 714.6 0 730 168 709 714.6 0 709 169 395 714.6 0 395 170 173 714.6 0 173 171 467 714.6 0 467 172 652 714.6 0 652 173 244 714.6 0 244 174 168 714.6 0 168 175 122 714.6 0 122 176 113 714.6 0 113 177 132 714.6 0 132 178 145 714.6 0 145 179 158 714.6 0 158 180 168 714.6 0 168 181 162 714.6 0 162 182 150 714.6 0 150 183 293 714.6 0 293 184 287 714.6 0 287 185 166 714.6 0 166 186 98 714.6 0 98 187 351 714.6 0 351 188 235 714.6 0 235 189 417 714.6 0 417 190 255 714.6 0 255 191 175 714.6 0 175 192 130 714.6 0 130 193 96 714.6 0 96 194 116 714.6 0 116 195 150 714.6 0 150 196 190 714.6 0 190 197 140 714.6 0 140 198 121 714.6 0 121 199 116 714.6 0 116 200 440 714.6 0 440 201 1634 714.6 0 1634 202 618 714.6 0 618 203 172 714.6 0 172 204 155 714.6 0 155 205 147 714.6 0 147 206 143 714.6 0 143 207 112 714.6 0 112 208 126 714.6 0 126 209 104 714.6 0 104 210 105 714.6 0 105 211 140 714.6 0 140 212 159 714.6 0 159 213 140 714.6 0 140 214 158 714.6 0 158 215 221 714.6 0 221 216 197 714.6 0 197 217 216 714.6 0 216 218 145 714.6 0 145 219 134 714.6 0 134 220 119 714.6 0 119 221 140 714.6 0 140 222 204 714.6 0 204 223 215 714.6 0 215 224 251 714.6 0 251 225 286 714.6 0 286 226 350 714.6 0 350 227 514 714.6 0 514 228 2554 714.6 0 2554 229 2813 714.6 0 2813 230 1612 714.6 0 1612 231 249 714.6 0 249 232 313 714.6 0 313 233 138 714.6 0 138 234 147 714.6 0 147 235 90 714.6 0 90 236 101 714.6 0 101 237 95 714.6 0 95 238 153 714.6 0 153 239 117 714.6 0 117 240 161 714.6 0 161 241 111 714.6 0 111 242 135 714.6 0 135 243 196 714.6 0 196 244 197 714.6 0 197 245 185 714.6 0 185 246 208 714.6 0 208 247 110 714.6 0 110 248 96 714.6 0 96 249 136 714.6 0 136 250 239 714.6 0 239 251 510 714.6 0 510 252 201 714.6 0 201 253 227 714.6 0 227 254 191 714.6 0 191 255 204 714.6 0 204 256 220 714.6 0 220 257 235 714.6 0 235 258 242 714.6 0 242 259 158 714.6 0 158 260 201 714.6 0 201 261 706 714.6 0 706 262 4110 714.6 0 4110 263 1015 714.6 0 1015 264 491 714.6 0 491 265 1342 714.6 0 1342 266 208 714.6 0 208 267 99 714.6 0 99 268 377 714.6 0 377 269 1677 714.6 0 1677 270 700 714.6 0 700 271 260 714.6 0 260 272 123 714.6 0 123 273 92 714.6 0 92 274 70 714.6 0 70 275 100 714.6 0 100 276 145 714.6 0 145 277 336 714.6 0 336 278 163 714.6 0 163 279 94 714.6 0 94 280 89 714.6 0 89 281 170 714.6 0 170 282 235 714.6 0 235 283 335 714.6 0 335 284 177 714.6 0 177 285 107 714.6 0 107 286 68 714.6 0 68 287 98 714.6 0 98 288 115 714.6 0 115 289 125 714.6 0 125 290 198 714.6 0 198 291 178 714.6 0 178 292 186 714.6 0 186 293 176 714.6 0 176 294 98 714.6 0 98 295 58 714.6 0 58 296 86 714.6 0 86 297 116 714.6 0 116 298 128 714.6 0 128 299 157 714.6 0 157 300 398 714.6 0 398 301 135 714.6 0 135 Finished in 1.39 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 36,612 Reads with adapters: 9,759 (26.7%) Reads that were too short: 21 (0.1%) Reads written (passing filters): 9,738 (26.6%) Total basepairs processed: 7,373,023 bp Total written (filtered): 2,693,464 bp (36.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9759 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 80 0.0 3 0 68 7 5 17 9579 0.0 3 9326 212 34 7 18 30 0.0 3 1 23 4 2 19 13 0.0 3 0 0 7 6 20 7 0.0 3 0 0 0 7 25 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 46 3 0.0 3 0 0 0 3 50 3 0.0 3 0 0 0 3 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 71 4 0.0 3 0 0 0 4 72 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 96 3 0.0 3 0 0 0 3 100 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 1 1 121 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.35 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 9,738 Reads with adapters: 726 (7.5%) Reads written (passing filters): 9,738 (100.0%) Total basepairs processed: 2,693,464 bp Total written (filtered): 2,691,284 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 726 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.3% C: 58.8% G: 9.2% T: 4.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 725 152.2 0 725 5 1 9.5 0 1