This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.88 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 54,759 (1.9%) Reads that were too short: 5,499 (0.2%) Reads written (passing filters): 49,260 (1.7%) Total basepairs processed: 881,011,047 bp Total written (filtered): 12,742,398 bp (1.4%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 54759 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 296 714.6 0 296 7 727 714.6 0 727 8 22050 714.6 0 22050 9 9298 714.6 0 9298 10 93 714.6 0 93 11 58 714.6 0 58 12 72 714.6 0 72 13 60 714.6 0 60 14 65 714.6 0 65 15 63 714.6 0 63 16 65 714.6 0 65 17 56 714.6 0 56 18 63 714.6 0 63 19 74 714.6 0 74 20 62 714.6 0 62 21 63 714.6 0 63 22 65 714.6 0 65 23 59 714.6 0 59 24 80 714.6 0 80 25 49 714.6 0 49 26 71 714.6 0 71 27 59 714.6 0 59 28 66 714.6 0 66 29 54 714.6 0 54 30 64 714.6 0 64 31 69 714.6 0 69 32 47 714.6 0 47 33 52 714.6 0 52 34 49 714.6 0 49 35 68 714.6 0 68 36 54 714.6 0 54 37 56 714.6 0 56 38 59 714.6 0 59 39 52 714.6 0 52 40 42 714.6 0 42 41 49 714.6 0 49 42 61 714.6 0 61 43 53 714.6 0 53 44 63 714.6 0 63 45 59 714.6 0 59 46 48 714.6 0 48 47 55 714.6 0 55 48 46 714.6 0 46 49 48 714.6 0 48 50 39 714.6 0 39 51 50 714.6 0 50 52 50 714.6 0 50 53 50 714.6 0 50 54 40 714.6 0 40 55 45 714.6 0 45 56 42 714.6 0 42 57 31 714.6 0 31 58 47 714.6 0 47 59 42 714.6 0 42 60 54 714.6 0 54 61 34 714.6 0 34 62 33 714.6 0 33 63 36 714.6 0 36 64 26 714.6 0 26 65 42 714.6 0 42 66 34 714.6 0 34 67 42 714.6 0 42 68 56 714.6 0 56 69 23 714.6 0 23 70 35 714.6 0 35 71 25 714.6 0 25 72 29 714.6 0 29 73 22 714.6 0 22 74 20 714.6 0 20 75 30 714.6 0 30 76 24 714.6 0 24 77 25 714.6 0 25 78 21 714.6 0 21 79 22 714.6 0 22 80 15 714.6 0 15 81 20 714.6 0 20 82 36 714.6 0 36 83 28 714.6 0 28 84 39 714.6 0 39 85 31 714.6 0 31 86 26 714.6 0 26 87 29 714.6 0 29 88 40 714.6 0 40 89 23 714.6 0 23 90 38 714.6 0 38 91 109 714.6 0 109 92 54 714.6 0 54 93 35 714.6 0 35 94 45 714.6 0 45 95 47 714.6 0 47 96 37 714.6 0 37 97 96 714.6 0 96 98 264 714.6 0 264 99 76 714.6 0 76 100 25 714.6 0 25 101 32 714.6 0 32 102 35 714.6 0 35 103 26 714.6 0 26 104 71 714.6 0 71 105 40 714.6 0 40 106 43 714.6 0 43 107 48 714.6 0 48 108 51 714.6 0 51 109 50 714.6 0 50 110 216 714.6 0 216 111 4469 714.6 0 4469 112 204 714.6 0 204 113 219 714.6 0 219 114 51 714.6 0 51 115 143 714.6 0 143 116 197 714.6 0 197 117 229 714.6 0 229 118 1461 714.6 0 1461 119 841 714.6 0 841 120 24 714.6 0 24 121 27 714.6 0 27 122 34 714.6 0 34 123 21 714.6 0 21 124 28 714.6 0 28 125 35 714.6 0 35 126 56 714.6 0 56 127 81 714.6 0 81 128 37 714.6 0 37 129 22 714.6 0 22 130 22 714.6 0 22 131 32 714.6 0 32 132 38 714.6 0 38 133 55 714.6 0 55 134 25 714.6 0 25 135 26 714.6 0 26 136 25 714.6 0 25 137 33 714.6 0 33 138 54 714.6 0 54 139 38 714.6 0 38 140 29 714.6 0 29 141 30 714.6 0 30 142 29 714.6 0 29 143 32 714.6 0 32 144 38 714.6 0 38 145 38 714.6 0 38 146 56 714.6 0 56 147 54 714.6 0 54 148 44 714.6 0 44 149 38 714.6 0 38 150 28 714.6 0 28 151 101 714.6 0 101 152 460 714.6 0 460 153 101 714.6 0 101 154 52 714.6 0 52 155 36 714.6 0 36 156 28 714.6 0 28 157 38 714.6 0 38 158 15 714.6 0 15 159 39 714.6 0 39 160 22 714.6 0 22 161 35 714.6 0 35 162 60 714.6 0 60 163 57 714.6 0 57 164 48 714.6 0 48 165 36 714.6 0 36 166 37 714.6 0 37 167 48 714.6 0 48 168 26 714.6 0 26 169 37 714.6 0 37 170 34 714.6 0 34 171 44 714.6 0 44 172 43 714.6 0 43 173 41 714.6 0 41 174 31 714.6 0 31 175 33 714.6 0 33 176 34 714.6 0 34 177 41 714.6 0 41 178 28 714.6 0 28 179 55 714.6 0 55 180 198 714.6 0 198 181 124 714.6 0 124 182 118 714.6 0 118 183 50 714.6 0 50 184 51 714.6 0 51 185 36 714.6 0 36 186 21 714.6 0 21 187 30 714.6 0 30 188 29 714.6 0 29 189 33 714.6 0 33 190 40 714.6 0 40 191 33 714.6 0 33 192 43 714.6 0 43 193 25 714.6 0 25 194 26 714.6 0 26 195 31 714.6 0 31 196 27 714.6 0 27 197 42 714.6 0 42 198 21 714.6 0 21 199 49 714.6 0 49 200 52 714.6 0 52 201 22 714.6 0 22 202 28 714.6 0 28 203 18 714.6 0 18 204 36 714.6 0 36 205 45 714.6 0 45 206 28 714.6 0 28 207 19 714.6 0 19 208 31 714.6 0 31 209 40 714.6 0 40 210 35 714.6 0 35 211 43 714.6 0 43 212 36 714.6 0 36 213 61 714.6 0 61 214 40 714.6 0 40 215 46 714.6 0 46 216 60 714.6 0 60 217 71 714.6 0 71 218 235 714.6 0 235 219 355 714.6 0 355 220 64 714.6 0 64 221 52 714.6 0 52 222 37 714.6 0 37 223 59 714.6 0 59 224 77 714.6 0 77 225 70 714.6 0 70 226 65 714.6 0 65 227 45 714.6 0 45 228 45 714.6 0 45 229 86 714.6 0 86 230 48 714.6 0 48 231 70 714.6 0 70 232 73 714.6 0 73 233 117 714.6 0 117 234 86 714.6 0 86 235 89 714.6 0 89 236 103 714.6 0 103 237 122 714.6 0 122 238 75 714.6 0 75 239 70 714.6 0 70 240 31 714.6 0 31 241 32 714.6 0 32 242 32 714.6 0 32 243 22 714.6 0 22 244 42 714.6 0 42 245 71 714.6 0 71 246 60 714.6 0 60 247 37 714.6 0 37 248 52 714.6 0 52 249 78 714.6 0 78 250 61 714.6 0 61 251 24 714.6 0 24 252 37 714.6 0 37 253 40 714.6 0 40 254 110 714.6 0 110 255 120 714.6 0 120 256 82 714.6 0 82 257 51 714.6 0 51 258 36 714.6 0 36 259 55 714.6 0 55 260 38 714.6 0 38 261 27 714.6 0 27 262 30 714.6 0 30 263 37 714.6 0 37 264 52 714.6 0 52 265 65 714.6 0 65 266 43 714.6 0 43 267 34 714.6 0 34 268 42 714.6 0 42 269 35 714.6 0 35 270 33 714.6 0 33 271 22 714.6 0 22 272 27 714.6 0 27 273 50 714.6 0 50 274 36 714.6 0 36 275 36 714.6 0 36 276 38 714.6 0 38 277 60 714.6 0 60 278 42 714.6 0 42 279 48 714.6 0 48 280 52 714.6 0 52 281 68 714.6 0 68 282 44 714.6 0 44 283 54 714.6 0 54 284 51 714.6 0 51 285 57 714.6 0 57 286 55 714.6 0 55 287 47 714.6 0 47 288 65 714.6 0 65 289 56 714.6 0 56 290 49 714.6 0 49 291 33 714.6 0 33 292 43 714.6 0 43 293 54 714.6 0 54 294 40 714.6 0 40 295 26 714.6 0 26 296 28 714.6 0 28 297 18 714.6 0 18 298 26 714.6 0 26 299 24 714.6 0 24 300 50 714.6 0 50 301 11 714.6 0 11 Finished in 2.46 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 49,260 Reads with adapters: 9,766 (19.8%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 9,761 (19.8%) Total basepairs processed: 12,742,398 bp Total written (filtered): 2,655,650 bp (20.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9766 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 11 0.0 3 0 0 0 0 11 18 8 0.0 3 0 0 6 1 1 19 126 0.0 3 0 123 3 20 9577 0.0 4 9450 122 3 1 1 21 26 0.0 4 7 12 1 0 6 22 5 0.0 4 0 0 1 1 3 23 5 0.0 4 0 0 0 3 2 31 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 196 1 0.0 4 1 228 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.32 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 9,761 Reads with adapters: 270 (2.8%) Reads written (passing filters): 9,761 (100.0%) Total basepairs processed: 2,655,650 bp Total written (filtered): 2,652,233 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 270 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.2% C: 3.3% G: 94.1% T: 0.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 152.5 0 1 4 3 38.1 0 3 9 20 0.0 0 2 18 10 158 0.0 1 7 151 11 85 0.0 1 1 84 223 2 0.0 1 2 261 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.