This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 80.37 s (27 us/read; 2.19 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 555,101 (19.0%) Reads that were too short: 39,375 (1.3%) Reads written (passing filters): 515,726 (17.6%) Total basepairs processed: 881,011,047 bp Total written (filtered): 135,722,534 bp (15.4%) === Adapter 1 === Sequence: AATCCT; Type: regular 5'; Length: 6; Trimmed: 555101 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 207 714.6 0 207 7 202 714.6 0 202 8 435 714.6 0 435 9 17825 714.6 0 17825 10 211 714.6 0 211 11 186 714.6 0 186 12 367 714.6 0 367 13 769 714.6 0 769 14 141 714.6 0 141 15 399 714.6 0 399 16 142 714.6 0 142 17 224 714.6 0 224 18 564 714.6 0 564 19 309 714.6 0 309 20 331 714.6 0 331 21 146 714.6 0 146 22 327 714.6 0 327 23 623 714.6 0 623 24 1070 714.6 0 1070 25 1563 714.6 0 1563 26 2137 714.6 0 2137 27 3214 714.6 0 3214 28 5806 714.6 0 5806 29 10672 714.6 0 10672 30 195439 714.6 0 195439 31 171708 714.6 0 171708 32 57684 714.6 0 57684 33 444 714.6 0 444 34 60 714.6 0 60 35 32 714.6 0 32 36 56 714.6 0 56 37 139 714.6 0 139 38 35 714.6 0 35 39 15 714.6 0 15 40 25 714.6 0 25 41 20 714.6 0 20 42 22 714.6 0 22 43 17 714.6 0 17 44 35 714.6 0 35 45 15 714.6 0 15 46 26 714.6 0 26 47 29 714.6 0 29 48 13 714.6 0 13 49 19 714.6 0 19 50 28 714.6 0 28 51 16 714.6 0 16 52 22 714.6 0 22 53 26 714.6 0 26 54 75 714.6 0 75 55 1537 714.6 0 1537 56 261 714.6 0 261 57 16 714.6 0 16 58 7 714.6 0 7 59 9 714.6 0 9 60 13 714.6 0 13 61 15 714.6 0 15 62 12 714.6 0 12 63 9 714.6 0 9 64 8 714.6 0 8 65 30 714.6 0 30 66 12 714.6 0 12 67 13 714.6 0 13 68 9 714.6 0 9 69 32 714.6 0 32 70 77 714.6 0 77 71 256 714.6 0 256 72 170 714.6 0 170 73 85 714.6 0 85 74 159 714.6 0 159 75 93 714.6 0 93 76 148 714.6 0 148 77 198 714.6 0 198 78 121 714.6 0 121 79 145 714.6 0 145 80 241 714.6 0 241 81 308 714.6 0 308 82 311 714.6 0 311 83 307 714.6 0 307 84 245 714.6 0 245 85 321 714.6 0 321 86 301 714.6 0 301 87 652 714.6 0 652 88 425 714.6 0 425 89 532 714.6 0 532 90 252 714.6 0 252 91 211 714.6 0 211 92 371 714.6 0 371 93 248 714.6 0 248 94 115 714.6 0 115 95 158 714.6 0 158 96 171 714.6 0 171 97 148 714.6 0 148 98 133 714.6 0 133 99 110 714.6 0 110 100 287 714.6 0 287 101 218 714.6 0 218 102 1013 714.6 0 1013 103 782 714.6 0 782 104 612 714.6 0 612 105 875 714.6 0 875 106 501 714.6 0 501 107 309 714.6 0 309 108 205 714.6 0 205 109 293 714.6 0 293 110 350 714.6 0 350 111 275 714.6 0 275 112 277 714.6 0 277 113 264 714.6 0 264 114 180 714.6 0 180 115 158 714.6 0 158 116 159 714.6 0 159 117 169 714.6 0 169 118 438 714.6 0 438 119 456 714.6 0 456 120 267 714.6 0 267 121 163 714.6 0 163 122 279 714.6 0 279 123 290 714.6 0 290 124 683 714.6 0 683 125 801 714.6 0 801 126 1052 714.6 0 1052 127 227 714.6 0 227 128 186 714.6 0 186 129 136 714.6 0 136 130 147 714.6 0 147 131 238 714.6 0 238 132 210 714.6 0 210 133 278 714.6 0 278 134 210 714.6 0 210 135 166 714.6 0 166 136 222 714.6 0 222 137 331 714.6 0 331 138 390 714.6 0 390 139 368 714.6 0 368 140 214 714.6 0 214 141 259 714.6 0 259 142 501 714.6 0 501 143 383 714.6 0 383 144 267 714.6 0 267 145 247 714.6 0 247 146 223 714.6 0 223 147 286 714.6 0 286 148 253 714.6 0 253 149 511 714.6 0 511 150 386 714.6 0 386 151 273 714.6 0 273 152 272 714.6 0 272 153 251 714.6 0 251 154 267 714.6 0 267 155 221 714.6 0 221 156 177 714.6 0 177 157 389 714.6 0 389 158 259 714.6 0 259 159 653 714.6 0 653 160 887 714.6 0 887 161 360 714.6 0 360 162 308 714.6 0 308 163 497 714.6 0 497 164 491 714.6 0 491 165 281 714.6 0 281 166 301 714.6 0 301 167 484 714.6 0 484 168 257 714.6 0 257 169 249 714.6 0 249 170 198 714.6 0 198 171 199 714.6 0 199 172 250 714.6 0 250 173 195 714.6 0 195 174 182 714.6 0 182 175 215 714.6 0 215 176 202 714.6 0 202 177 263 714.6 0 263 178 243 714.6 0 243 179 379 714.6 0 379 180 292 714.6 0 292 181 199 714.6 0 199 182 266 714.6 0 266 183 343 714.6 0 343 184 400 714.6 0 400 185 299 714.6 0 299 186 234 714.6 0 234 187 129 714.6 0 129 188 288 714.6 0 288 189 383 714.6 0 383 190 298 714.6 0 298 191 212 714.6 0 212 192 170 714.6 0 170 193 131 714.6 0 131 194 199 714.6 0 199 195 186 714.6 0 186 196 179 714.6 0 179 197 299 714.6 0 299 198 200 714.6 0 200 199 310 714.6 0 310 200 350 714.6 0 350 201 296 714.6 0 296 202 382 714.6 0 382 203 423 714.6 0 423 204 575 714.6 0 575 205 477 714.6 0 477 206 563 714.6 0 563 207 289 714.6 0 289 208 184 714.6 0 184 209 133 714.6 0 133 210 184 714.6 0 184 211 186 714.6 0 186 212 196 714.6 0 196 213 228 714.6 0 228 214 254 714.6 0 254 215 324 714.6 0 324 216 242 714.6 0 242 217 192 714.6 0 192 218 296 714.6 0 296 219 278 714.6 0 278 220 251 714.6 0 251 221 125 714.6 0 125 222 213 714.6 0 213 223 305 714.6 0 305 224 385 714.6 0 385 225 317 714.6 0 317 226 469 714.6 0 469 227 275 714.6 0 275 228 464 714.6 0 464 229 284 714.6 0 284 230 202 714.6 0 202 231 275 714.6 0 275 232 404 714.6 0 404 233 323 714.6 0 323 234 242 714.6 0 242 235 132 714.6 0 132 236 107 714.6 0 107 237 100 714.6 0 100 238 97 714.6 0 97 239 142 714.6 0 142 240 195 714.6 0 195 241 233 714.6 0 233 242 274 714.6 0 274 243 328 714.6 0 328 244 221 714.6 0 221 245 232 714.6 0 232 246 164 714.6 0 164 247 151 714.6 0 151 248 64 714.6 0 64 249 150 714.6 0 150 250 272 714.6 0 272 251 357 714.6 0 357 252 622 714.6 0 622 253 522 714.6 0 522 254 1724 714.6 0 1724 255 960 714.6 0 960 256 911 714.6 0 911 257 430 714.6 0 430 258 368 714.6 0 368 259 360 714.6 0 360 260 1002 714.6 0 1002 261 5376 714.6 0 5376 262 4007 714.6 0 4007 263 468 714.6 0 468 264 362 714.6 0 362 265 411 714.6 0 411 266 157 714.6 0 157 267 132 714.6 0 132 268 143 714.6 0 143 269 151 714.6 0 151 270 171 714.6 0 171 271 243 714.6 0 243 272 350 714.6 0 350 273 242 714.6 0 242 274 207 714.6 0 207 275 922 714.6 0 922 276 461 714.6 0 461 277 824 714.6 0 824 278 796 714.6 0 796 279 402 714.6 0 402 280 156 714.6 0 156 281 187 714.6 0 187 282 235 714.6 0 235 283 332 714.6 0 332 284 263 714.6 0 263 285 116 714.6 0 116 286 139 714.6 0 139 287 87 714.6 0 87 288 55 714.6 0 55 289 137 714.6 0 137 290 212 714.6 0 212 291 319 714.6 0 319 292 267 714.6 0 267 293 147 714.6 0 147 294 81 714.6 0 81 295 93 714.6 0 93 296 78 714.6 0 78 297 106 714.6 0 106 298 97 714.6 0 97 299 122 714.6 0 122 300 164 714.6 0 164 301 99 714.6 0 99 Finished in 21.05 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 515,726 Reads with adapters: 19,011 (3.7%) Reads that were too short: 375 (0.1%) Reads written (passing filters): 18,636 (3.6%) Total basepairs processed: 135,722,534 bp Total written (filtered): 5,104,483 bp (3.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 19011 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 5 2 16 195 0.0 3 0 186 6 3 17 18170 0.0 3 17625 473 57 15 18 55 0.0 3 2 41 7 5 19 17 0.0 3 0 0 12 5 20 54 0.0 3 34 1 0 19 21 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 28 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 67 18 0.0 3 0 0 1 17 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 70 13 0.0 3 0 0 0 13 71 3 0.0 3 0 0 0 3 73 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 76 3 0.0 3 0 0 0 3 79 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 1 87 2 0.0 3 0 0 0 2 94 2 0.0 3 0 0 0 2 96 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 117 3 0.0 3 0 0 0 3 120 8 0.0 3 0 0 0 8 123 2 0.0 3 0 0 0 2 130 2 0.0 3 0 0 0 2 132 2 0.0 3 0 0 0 2 133 4 0.0 3 0 0 0 4 136 1 0.0 3 0 0 0 1 137 3 0.0 3 0 0 0 3 138 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 141 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 1 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 168 3 0.0 3 0 0 0 3 169 33 0.0 3 0 0 1 32 170 1 0.0 3 0 0 0 1 171 6 0.0 3 0 0 0 6 172 4 0.0 3 0 0 0 4 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 19 0.0 3 0 0 0 19 183 4 0.0 3 0 0 0 4 184 1 0.0 3 0 0 0 1 194 4 0.0 3 0 0 0 4 195 11 0.0 3 0 0 0 11 197 1 0.0 3 0 0 0 1 198 26 0.0 3 0 0 0 26 199 1 0.0 3 0 0 0 1 202 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 232 4 0.0 3 0 0 0 4 237 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 247 18 0.0 3 0 0 0 18 256 7 0.0 3 0 0 0 7 257 27 0.0 3 0 0 0 27 258 64 0.0 3 0 0 0 64 259 83 0.0 3 0 0 2 81 260 25 0.0 3 0 0 0 25 262 4 0.0 3 0 0 0 4 263 2 0.0 3 0 0 0 2 264 1 0.0 3 0 0 0 1 265 22 0.0 3 0 0 0 22 266 6 0.0 3 0 0 0 6 267 2 0.0 3 0 0 0 2 268 5 0.0 3 0 0 0 5 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.66 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 18,636 Reads with adapters: 532 (2.9%) Reads written (passing filters): 18,636 (100.0%) Total basepairs processed: 5,104,483 bp Total written (filtered): 5,102,875 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 532 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 24.1% C: 5.5% G: 67.7% T: 2.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 522 291.2 0 522 4 8 72.8 0 8 5 2 18.2 0 2