This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 79.20 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 394,473 (13.5%) Reads that were too short: 146,384 (5.0%) Reads written (passing filters): 248,089 (8.5%) Total basepairs processed: 881,011,047 bp Total written (filtered): 48,825,907 bp (5.5%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 394473 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 10143 714.6 0 10143 7 379 714.6 0 379 8 24729 714.6 0 24729 9 96 714.6 0 96 10 29 714.6 0 29 11 23 714.6 0 23 12 37 714.6 0 37 13 27 714.6 0 27 14 17 714.6 0 17 15 25 714.6 0 25 16 28 714.6 0 28 17 23 714.6 0 23 18 37 714.6 0 37 19 22 714.6 0 22 20 21 714.6 0 21 21 22 714.6 0 22 22 31 714.6 0 31 23 30 714.6 0 30 24 24 714.6 0 24 25 48 714.6 0 48 26 31 714.6 0 31 27 31 714.6 0 31 28 29 714.6 0 29 29 24 714.6 0 24 30 27 714.6 0 27 31 31 714.6 0 31 32 23 714.6 0 23 33 70 714.6 0 70 34 27 714.6 0 27 35 49 714.6 0 49 36 26 714.6 0 26 37 26 714.6 0 26 38 19 714.6 0 19 39 28 714.6 0 28 40 22 714.6 0 22 41 27 714.6 0 27 42 55 714.6 0 55 43 23 714.6 0 23 44 23 714.6 0 23 45 28 714.6 0 28 46 26 714.6 0 26 47 25 714.6 0 25 48 27 714.6 0 27 49 18 714.6 0 18 50 19 714.6 0 19 51 41 714.6 0 41 52 56 714.6 0 56 53 263 714.6 0 263 54 41271 714.6 0 41271 55 580 714.6 0 580 56 56 714.6 0 56 57 37 714.6 0 37 58 45 714.6 0 45 59 41 714.6 0 41 60 97 714.6 0 97 61 47 714.6 0 47 62 54 714.6 0 54 63 90 714.6 0 90 64 89 714.6 0 89 65 136 714.6 0 136 66 175 714.6 0 175 67 274 714.6 0 274 68 512 714.6 0 512 69 8998 714.6 0 8998 70 6734 714.6 0 6734 71 1198 714.6 0 1198 72 39 714.6 0 39 73 75 714.6 0 75 74 95 714.6 0 95 75 338 714.6 0 338 76 330 714.6 0 330 77 174 714.6 0 174 78 237 714.6 0 237 79 364 714.6 0 364 80 907 714.6 0 907 81 1044 714.6 0 1044 82 758 714.6 0 758 83 489 714.6 0 489 84 742 714.6 0 742 85 1030 714.6 0 1030 86 592 714.6 0 592 87 903 714.6 0 903 88 1735 714.6 0 1735 89 723 714.6 0 723 90 634 714.6 0 634 91 722 714.6 0 722 92 365 714.6 0 365 93 581 714.6 0 581 94 1045 714.6 0 1045 95 572 714.6 0 572 96 914 714.6 0 914 97 1001 714.6 0 1001 98 878 714.6 0 878 99 645 714.6 0 645 100 1575 714.6 0 1575 101 2204 714.6 0 2204 102 3243 714.6 0 3243 103 1591 714.6 0 1591 104 905 714.6 0 905 105 775 714.6 0 775 106 646 714.6 0 646 107 348 714.6 0 348 108 592 714.6 0 592 109 375 714.6 0 375 110 410 714.6 0 410 111 585 714.6 0 585 112 931 714.6 0 931 113 749 714.6 0 749 114 933 714.6 0 933 115 780 714.6 0 780 116 830 714.6 0 830 117 1204 714.6 0 1204 118 1692 714.6 0 1692 119 1124 714.6 0 1124 120 772 714.6 0 772 121 803 714.6 0 803 122 1101 714.6 0 1101 123 1299 714.6 0 1299 124 1225 714.6 0 1225 125 916 714.6 0 916 126 1009 714.6 0 1009 127 934 714.6 0 934 128 746 714.6 0 746 129 895 714.6 0 895 130 1142 714.6 0 1142 131 1381 714.6 0 1381 132 2275 714.6 0 2275 133 1921 714.6 0 1921 134 1502 714.6 0 1502 135 1055 714.6 0 1055 136 1396 714.6 0 1396 137 1822 714.6 0 1822 138 1827 714.6 0 1827 139 1525 714.6 0 1525 140 1143 714.6 0 1143 141 844 714.6 0 844 142 922 714.6 0 922 143 1283 714.6 0 1283 144 1290 714.6 0 1290 145 1023 714.6 0 1023 146 986 714.6 0 986 147 1156 714.6 0 1156 148 1643 714.6 0 1643 149 1090 714.6 0 1090 150 1344 714.6 0 1344 151 1427 714.6 0 1427 152 1100 714.6 0 1100 153 1098 714.6 0 1098 154 1167 714.6 0 1167 155 1006 714.6 0 1006 156 1445 714.6 0 1445 157 1837 714.6 0 1837 158 2021 714.6 0 2021 159 1632 714.6 0 1632 160 1488 714.6 0 1488 161 1346 714.6 0 1346 162 1268 714.6 0 1268 163 1263 714.6 0 1263 164 1353 714.6 0 1353 165 989 714.6 0 989 166 858 714.6 0 858 167 1140 714.6 0 1140 168 1598 714.6 0 1598 169 1692 714.6 0 1692 170 1384 714.6 0 1384 171 1344 714.6 0 1344 172 1282 714.6 0 1282 173 1094 714.6 0 1094 174 779 714.6 0 779 175 1109 714.6 0 1109 176 2082 714.6 0 2082 177 2690 714.6 0 2690 178 2261 714.6 0 2261 179 1734 714.6 0 1734 180 1434 714.6 0 1434 181 1227 714.6 0 1227 182 1383 714.6 0 1383 183 1489 714.6 0 1489 184 1386 714.6 0 1386 185 1468 714.6 0 1468 186 1414 714.6 0 1414 187 1740 714.6 0 1740 188 2177 714.6 0 2177 189 2152 714.6 0 2152 190 1129 714.6 0 1129 191 854 714.6 0 854 192 919 714.6 0 919 193 1164 714.6 0 1164 194 1091 714.6 0 1091 195 1738 714.6 0 1738 196 2214 714.6 0 2214 197 1196 714.6 0 1196 198 1087 714.6 0 1087 199 1093 714.6 0 1093 200 1495 714.6 0 1495 201 1739 714.6 0 1739 202 1218 714.6 0 1218 203 937 714.6 0 937 204 1252 714.6 0 1252 205 1415 714.6 0 1415 206 1246 714.6 0 1246 207 1216 714.6 0 1216 208 1087 714.6 0 1087 209 1417 714.6 0 1417 210 1314 714.6 0 1314 211 1398 714.6 0 1398 212 1382 714.6 0 1382 213 1542 714.6 0 1542 214 1556 714.6 0 1556 215 1542 714.6 0 1542 216 1357 714.6 0 1357 217 1592 714.6 0 1592 218 1547 714.6 0 1547 219 1443 714.6 0 1443 220 1266 714.6 0 1266 221 1585 714.6 0 1585 222 1979 714.6 0 1979 223 1780 714.6 0 1780 224 1728 714.6 0 1728 225 1501 714.6 0 1501 226 1625 714.6 0 1625 227 1368 714.6 0 1368 228 1198 714.6 0 1198 229 1412 714.6 0 1412 230 1431 714.6 0 1431 231 1089 714.6 0 1089 232 1091 714.6 0 1091 233 1256 714.6 0 1256 234 1264 714.6 0 1264 235 1141 714.6 0 1141 236 1047 714.6 0 1047 237 1909 714.6 0 1909 238 1840 714.6 0 1840 239 1291 714.6 0 1291 240 1294 714.6 0 1294 241 1542 714.6 0 1542 242 1489 714.6 0 1489 243 1214 714.6 0 1214 244 1922 714.6 0 1922 245 1887 714.6 0 1887 246 1564 714.6 0 1564 247 1356 714.6 0 1356 248 1553 714.6 0 1553 249 1596 714.6 0 1596 250 1647 714.6 0 1647 251 1155 714.6 0 1155 252 614 714.6 0 614 253 714 714.6 0 714 254 1231 714.6 0 1231 255 1512 714.6 0 1512 256 1827 714.6 0 1827 257 1606 714.6 0 1606 258 1633 714.6 0 1633 259 1825 714.6 0 1825 260 1600 714.6 0 1600 261 1245 714.6 0 1245 262 820 714.6 0 820 263 879 714.6 0 879 264 1117 714.6 0 1117 265 1368 714.6 0 1368 266 1935 714.6 0 1935 267 1462 714.6 0 1462 268 1344 714.6 0 1344 269 2242 714.6 0 2242 270 2625 714.6 0 2625 271 2128 714.6 0 2128 272 1186 714.6 0 1186 273 1511 714.6 0 1511 274 1411 714.6 0 1411 275 1566 714.6 0 1566 276 2407 714.6 0 2407 277 1940 714.6 0 1940 278 1104 714.6 0 1104 279 961 714.6 0 961 280 1149 714.6 0 1149 281 1878 714.6 0 1878 282 1906 714.6 0 1906 283 1368 714.6 0 1368 284 1255 714.6 0 1255 285 1276 714.6 0 1276 286 1196 714.6 0 1196 287 1542 714.6 0 1542 288 2253 714.6 0 2253 289 1384 714.6 0 1384 290 1016 714.6 0 1016 291 1097 714.6 0 1097 292 1243 714.6 0 1243 293 1496 714.6 0 1496 294 1878 714.6 0 1878 295 1872 714.6 0 1872 296 1380 714.6 0 1380 297 1389 714.6 0 1389 298 1592 714.6 0 1592 299 1621 714.6 0 1621 300 1228 714.6 0 1228 301 2071 714.6 0 2071 Finished in 8.67 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 248,089 Reads with adapters: 25,385 (10.2%) Reads that were too short: 119 (0.0%) Reads written (passing filters): 25,266 (10.2%) Total basepairs processed: 48,825,907 bp Total written (filtered): 6,948,876 bp (14.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 25385 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 9 0.0 3 0 0 5 4 16 188 0.0 3 0 165 17 6 17 17379 0.0 3 16615 664 77 23 18 7468 0.0 3 7189 233 38 8 19 53 0.0 3 1 24 16 12 20 34 0.0 3 0 0 5 29 21 11 0.0 3 0 0 0 11 22 2 0.0 3 0 0 0 2 23 2 0.0 3 1 0 0 1 27 1 0.0 3 0 0 0 1 28 3 0.0 3 0 0 0 3 30 2 0.0 3 0 0 0 2 31 6 0.0 3 0 0 0 6 33 1 0.0 3 0 0 0 1 34 7 0.0 3 0 0 0 7 35 2 0.0 3 1 0 0 1 36 3 0.0 3 0 0 0 3 37 8 0.0 3 0 0 0 8 38 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 45 3 0.0 3 0 0 0 3 46 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 0 3 49 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 7 0.0 3 0 0 0 7 57 3 0.0 3 0 0 0 3 58 2 0.0 3 0 0 0 2 59 3 0.0 3 0 0 1 2 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 79 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 87 1 0.0 3 0 0 0 1 91 14 0.0 3 0 0 0 14 92 4 0.0 3 0 0 0 4 93 1 0.0 3 0 0 0 1 94 5 0.0 3 0 0 1 4 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 5 0.0 3 0 0 1 4 102 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 104 4 0.0 3 0 0 0 4 105 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 3 0.0 3 0 0 0 3 115 4 0.0 3 0 0 0 4 116 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 120 3 0.0 3 0 0 0 3 121 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 15 0.0 3 0 0 0 15 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 165 6 0.0 3 0 0 0 6 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 200 2 0.0 3 0 0 0 2 214 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 219 7 0.0 3 0 0 0 7 220 6 0.0 3 0 0 1 5 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 229 2 0.0 3 0 0 0 2 236 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 240 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 271 2 0.0 3 0 0 0 2 283 6 0.0 3 0 0 0 6 284 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.87 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 25,266 Reads with adapters: 722 (2.9%) Reads written (passing filters): 25,266 (100.0%) Total basepairs processed: 6,948,876 bp Total written (filtered): 6,946,690 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 722 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 41.3% C: 11.8% G: 42.1% T: 4.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 703 394.8 0 703 4 18 98.7 0 18 5 1 24.7 0 1