This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.61 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 113,385 (3.9%) Reads that were too short: 12,509 (0.4%) Reads written (passing filters): 100,876 (3.4%) Total basepairs processed: 881,011,047 bp Total written (filtered): 22,645,227 bp (2.6%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 113385 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1684 714.6 0 1684 7 18524 714.6 0 18524 8 154 714.6 0 154 9 4111 714.6 0 4111 10 45 714.6 0 45 11 46 714.6 0 46 12 88 714.6 0 88 13 40 714.6 0 40 14 44 714.6 0 44 15 43 714.6 0 43 16 76 714.6 0 76 17 40 714.6 0 40 18 57 714.6 0 57 19 40 714.6 0 40 20 40 714.6 0 40 21 50 714.6 0 50 22 48 714.6 0 48 23 41 714.6 0 41 24 56 714.6 0 56 25 56 714.6 0 56 26 42 714.6 0 42 27 48 714.6 0 48 28 47 714.6 0 47 29 35 714.6 0 35 30 53 714.6 0 53 31 52 714.6 0 52 32 49 714.6 0 49 33 35 714.6 0 35 34 53 714.6 0 53 35 45 714.6 0 45 36 79 714.6 0 79 37 42 714.6 0 42 38 42 714.6 0 42 39 41 714.6 0 41 40 47 714.6 0 47 41 54 714.6 0 54 42 49 714.6 0 49 43 46 714.6 0 46 44 38 714.6 0 38 45 41 714.6 0 41 46 38 714.6 0 38 47 44 714.6 0 44 48 45 714.6 0 45 49 35 714.6 0 35 50 51 714.6 0 51 51 41 714.6 0 41 52 52 714.6 0 52 53 67 714.6 0 67 54 76 714.6 0 76 55 54 714.6 0 54 56 46 714.6 0 46 57 48 714.6 0 48 58 41 714.6 0 41 59 58 714.6 0 58 60 58 714.6 0 58 61 39 714.6 0 39 62 98 714.6 0 98 63 39 714.6 0 39 64 41 714.6 0 41 65 47 714.6 0 47 66 54 714.6 0 54 67 43 714.6 0 43 68 78 714.6 0 78 69 94 714.6 0 94 70 130 714.6 0 130 71 146 714.6 0 146 72 349 714.6 0 349 73 1187 714.6 0 1187 74 286 714.6 0 286 75 325 714.6 0 325 76 603 714.6 0 603 77 323 714.6 0 323 78 265 714.6 0 265 79 504 714.6 0 504 80 499 714.6 0 499 81 404 714.6 0 404 82 431 714.6 0 431 83 409 714.6 0 409 84 480 714.6 0 480 85 1064 714.6 0 1064 86 11921 714.6 0 11921 87 17257 714.6 0 17257 88 18825 714.6 0 18825 89 116 714.6 0 116 90 47 714.6 0 47 91 41 714.6 0 41 92 48 714.6 0 48 93 67 714.6 0 67 94 128 714.6 0 128 95 93 714.6 0 93 96 62 714.6 0 62 97 45 714.6 0 45 98 47 714.6 0 47 99 82 714.6 0 82 100 293 714.6 0 293 101 131 714.6 0 131 102 55 714.6 0 55 103 40 714.6 0 40 104 24 714.6 0 24 105 39 714.6 0 39 106 30 714.6 0 30 107 35 714.6 0 35 108 52 714.6 0 52 109 53 714.6 0 53 110 78 714.6 0 78 111 62 714.6 0 62 112 68 714.6 0 68 113 39 714.6 0 39 114 42 714.6 0 42 115 73 714.6 0 73 116 96 714.6 0 96 117 88 714.6 0 88 118 71 714.6 0 71 119 72 714.6 0 72 120 83 714.6 0 83 121 96 714.6 0 96 122 70 714.6 0 70 123 77 714.6 0 77 124 200 714.6 0 200 125 868 714.6 0 868 126 198 714.6 0 198 127 895 714.6 0 895 128 119 714.6 0 119 129 111 714.6 0 111 130 159 714.6 0 159 131 159 714.6 0 159 132 125 714.6 0 125 133 100 714.6 0 100 134 98 714.6 0 98 135 125 714.6 0 125 136 137 714.6 0 137 137 129 714.6 0 129 138 128 714.6 0 128 139 111 714.6 0 111 140 103 714.6 0 103 141 106 714.6 0 106 142 109 714.6 0 109 143 127 714.6 0 127 144 402 714.6 0 402 145 1319 714.6 0 1319 146 2272 714.6 0 2272 147 408 714.6 0 408 148 65 714.6 0 65 149 61 714.6 0 61 150 81 714.6 0 81 151 73 714.6 0 73 152 70 714.6 0 70 153 76 714.6 0 76 154 116 714.6 0 116 155 101 714.6 0 101 156 120 714.6 0 120 157 105 714.6 0 105 158 99 714.6 0 99 159 82 714.6 0 82 160 76 714.6 0 76 161 80 714.6 0 80 162 50 714.6 0 50 163 76 714.6 0 76 164 85 714.6 0 85 165 121 714.6 0 121 166 523 714.6 0 523 167 827 714.6 0 827 168 131 714.6 0 131 169 97 714.6 0 97 170 84 714.6 0 84 171 91 714.6 0 91 172 85 714.6 0 85 173 107 714.6 0 107 174 112 714.6 0 112 175 96 714.6 0 96 176 88 714.6 0 88 177 77 714.6 0 77 178 79 714.6 0 79 179 63 714.6 0 63 180 110 714.6 0 110 181 105 714.6 0 105 182 95 714.6 0 95 183 103 714.6 0 103 184 103 714.6 0 103 185 140 714.6 0 140 186 358 714.6 0 358 187 510 714.6 0 510 188 107 714.6 0 107 189 88 714.6 0 88 190 93 714.6 0 93 191 97 714.6 0 97 192 113 714.6 0 113 193 107 714.6 0 107 194 88 714.6 0 88 195 109 714.6 0 109 196 111 714.6 0 111 197 120 714.6 0 120 198 134 714.6 0 134 199 57 714.6 0 57 200 75 714.6 0 75 201 89 714.6 0 89 202 115 714.6 0 115 203 106 714.6 0 106 204 156 714.6 0 156 205 253 714.6 0 253 206 381 714.6 0 381 207 1099 714.6 0 1099 208 454 714.6 0 454 209 96 714.6 0 96 210 167 714.6 0 167 211 256 714.6 0 256 212 450 714.6 0 450 213 243 714.6 0 243 214 104 714.6 0 104 215 76 714.6 0 76 216 88 714.6 0 88 217 97 714.6 0 97 218 80 714.6 0 80 219 62 714.6 0 62 220 72 714.6 0 72 221 94 714.6 0 94 222 106 714.6 0 106 223 116 714.6 0 116 224 95 714.6 0 95 225 91 714.6 0 91 226 114 714.6 0 114 227 222 714.6 0 222 228 112 714.6 0 112 229 77 714.6 0 77 230 50 714.6 0 50 231 63 714.6 0 63 232 54 714.6 0 54 233 56 714.6 0 56 234 81 714.6 0 81 235 83 714.6 0 83 236 75 714.6 0 75 237 77 714.6 0 77 238 83 714.6 0 83 239 70 714.6 0 70 240 49 714.6 0 49 241 134 714.6 0 134 242 161 714.6 0 161 243 357 714.6 0 357 244 73 714.6 0 73 245 74 714.6 0 74 246 75 714.6 0 75 247 32 714.6 0 32 248 51 714.6 0 51 249 36 714.6 0 36 250 50 714.6 0 50 251 86 714.6 0 86 252 132 714.6 0 132 253 110 714.6 0 110 254 94 714.6 0 94 255 56 714.6 0 56 256 73 714.6 0 73 257 57 714.6 0 57 258 47 714.6 0 47 259 47 714.6 0 47 260 86 714.6 0 86 261 93 714.6 0 93 262 133 714.6 0 133 263 116 714.6 0 116 264 87 714.6 0 87 265 73 714.6 0 73 266 85 714.6 0 85 267 44 714.6 0 44 268 39 714.6 0 39 269 33 714.6 0 33 270 66 714.6 0 66 271 70 714.6 0 70 272 87 714.6 0 87 273 128 714.6 0 128 274 100 714.6 0 100 275 84 714.6 0 84 276 94 714.6 0 94 277 67 714.6 0 67 278 101 714.6 0 101 279 82 714.6 0 82 280 41 714.6 0 41 281 46 714.6 0 46 282 77 714.6 0 77 283 71 714.6 0 71 284 64 714.6 0 64 285 77 714.6 0 77 286 60 714.6 0 60 287 50 714.6 0 50 288 55 714.6 0 55 289 40 714.6 0 40 290 61 714.6 0 61 291 139 714.6 0 139 292 104 714.6 0 104 293 60 714.6 0 60 294 51 714.6 0 51 295 51 714.6 0 51 296 103 714.6 0 103 297 1154 714.6 0 1154 298 135 714.6 0 135 299 138 714.6 0 138 300 136 714.6 0 136 301 260 714.6 0 260 Finished in 4.53 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 100,876 Reads with adapters: 19,376 (19.2%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 19,369 (19.2%) Total basepairs processed: 22,645,227 bp Total written (filtered): 5,304,064 bp (23.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 19376 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 1 0.0 3 0 0 0 0 1 18 12 0.0 3 0 0 12 19 430 0.0 3 0 422 7 0 1 20 18389 0.0 4 18152 228 6 0 3 21 34 0.0 4 8 26 22 462 0.0 4 448 7 3 0 4 23 10 0.0 4 1 1 0 7 1 24 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 75 1 0.0 4 1 84 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 2 127 9 0.0 4 0 0 0 0 9 128 1 0.0 4 0 0 0 0 1 129 3 0.0 4 0 0 0 0 3 135 10 0.0 4 0 0 0 0 10 140 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 149 2 0.0 4 0 0 0 0 2 155 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.66 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 19,369 Reads with adapters: 1,338 (6.9%) Reads written (passing filters): 19,369 (100.0%) Total basepairs processed: 5,304,064 bp Total written (filtered): 5,025,516 bp (94.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1338 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 69.4% C: 12.5% G: 18.0% T: 0.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 12 302.6 0 12 10 2 0.0 1 0 2 11 238 0.0 1 0 238 12 4 0.0 1 0 4 14 1 0.0 1 0 1 16 1 0.0 1 0 1 225 152 0.0 1 138 14 226 11 0.0 1 11 234 12 0.0 1 11 1 257 2 0.0 1 2 259 1 0.0 1 1 260 2 0.0 1 2 261 813 0.0 1 762 51 262 84 0.0 1 73 11 264 1 0.0 1 1 272 2 0.0 1 2