This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.31 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 36,529 (1.2%) Reads that were too short: 5,291 (0.2%) Reads written (passing filters): 31,238 (1.1%) Total basepairs processed: 881,011,047 bp Total written (filtered): 7,252,039 bp (0.8%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 36529 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1207 714.6 0 1207 7 936 714.6 0 936 8 3301 714.6 0 3301 9 5294 714.6 0 5294 10 66 714.6 0 66 11 46 714.6 0 46 12 39 714.6 0 39 13 67 714.6 0 67 14 49 714.6 0 49 15 42 714.6 0 42 16 59 714.6 0 59 17 47 714.6 0 47 18 53 714.6 0 53 19 56 714.6 0 56 20 48 714.6 0 48 21 62 714.6 0 62 22 58 714.6 0 58 23 53 714.6 0 53 24 66 714.6 0 66 25 56 714.6 0 56 26 67 714.6 0 67 27 80 714.6 0 80 28 69 714.6 0 69 29 51 714.6 0 51 30 51 714.6 0 51 31 54 714.6 0 54 32 78 714.6 0 78 33 79 714.6 0 79 34 78 714.6 0 78 35 66 714.6 0 66 36 76 714.6 0 76 37 803 714.6 0 803 38 1381 714.6 0 1381 39 1623 714.6 0 1623 40 52 714.6 0 52 41 35 714.6 0 35 42 67 714.6 0 67 43 62 714.6 0 62 44 59 714.6 0 59 45 57 714.6 0 57 46 53 714.6 0 53 47 48 714.6 0 48 48 43 714.6 0 43 49 56 714.6 0 56 50 51 714.6 0 51 51 43 714.6 0 43 52 54 714.6 0 54 53 53 714.6 0 53 54 63 714.6 0 63 55 49 714.6 0 49 56 44 714.6 0 44 57 60 714.6 0 60 58 61 714.6 0 61 59 106 714.6 0 106 60 48 714.6 0 48 61 48 714.6 0 48 62 220 714.6 0 220 63 167 714.6 0 167 64 36 714.6 0 36 65 36 714.6 0 36 66 43 714.6 0 43 67 41 714.6 0 41 68 60 714.6 0 60 69 46 714.6 0 46 70 78 714.6 0 78 71 42 714.6 0 42 72 46 714.6 0 46 73 57 714.6 0 57 74 59 714.6 0 59 75 74 714.6 0 74 76 81 714.6 0 81 77 118 714.6 0 118 78 47 714.6 0 47 79 41 714.6 0 41 80 39 714.6 0 39 81 48 714.6 0 48 82 53 714.6 0 53 83 49 714.6 0 49 84 18 714.6 0 18 85 36 714.6 0 36 86 29 714.6 0 29 87 38 714.6 0 38 88 47 714.6 0 47 89 48 714.6 0 48 90 40 714.6 0 40 91 91 714.6 0 91 92 356 714.6 0 356 93 356 714.6 0 356 94 67 714.6 0 67 95 48 714.6 0 48 96 49 714.6 0 49 97 51 714.6 0 51 98 49 714.6 0 49 99 43 714.6 0 43 100 40 714.6 0 40 101 34 714.6 0 34 102 38 714.6 0 38 103 43 714.6 0 43 104 41 714.6 0 41 105 34 714.6 0 34 106 60 714.6 0 60 107 42 714.6 0 42 108 49 714.6 0 49 109 52 714.6 0 52 110 40 714.6 0 40 111 51 714.6 0 51 112 64 714.6 0 64 113 79 714.6 0 79 114 142 714.6 0 142 115 94 714.6 0 94 116 81 714.6 0 81 117 39 714.6 0 39 118 54 714.6 0 54 119 57 714.6 0 57 120 32 714.6 0 32 121 70 714.6 0 70 122 64 714.6 0 64 123 46 714.6 0 46 124 42 714.6 0 42 125 37 714.6 0 37 126 48 714.6 0 48 127 60 714.6 0 60 128 71 714.6 0 71 129 322 714.6 0 322 130 241 714.6 0 241 131 187 714.6 0 187 132 99 714.6 0 99 133 56 714.6 0 56 134 58 714.6 0 58 135 68 714.6 0 68 136 91 714.6 0 91 137 70 714.6 0 70 138 78 714.6 0 78 139 84 714.6 0 84 140 88 714.6 0 88 141 117 714.6 0 117 142 1449 714.6 0 1449 143 1921 714.6 0 1921 144 2044 714.6 0 2044 145 36 714.6 0 36 146 41 714.6 0 41 147 23 714.6 0 23 148 42 714.6 0 42 149 47 714.6 0 47 150 54 714.6 0 54 151 66 714.6 0 66 152 40 714.6 0 40 153 53 714.6 0 53 154 44 714.6 0 44 155 51 714.6 0 51 156 44 714.6 0 44 157 51 714.6 0 51 158 53 714.6 0 53 159 38 714.6 0 38 160 38 714.6 0 38 161 29 714.6 0 29 162 35 714.6 0 35 163 41 714.6 0 41 164 48 714.6 0 48 165 36 714.6 0 36 166 47 714.6 0 47 167 41 714.6 0 41 168 100 714.6 0 100 169 59 714.6 0 59 170 117 714.6 0 117 171 30 714.6 0 30 172 35 714.6 0 35 173 40 714.6 0 40 174 37 714.6 0 37 175 40 714.6 0 40 176 31 714.6 0 31 177 48 714.6 0 48 178 48 714.6 0 48 179 38 714.6 0 38 180 31 714.6 0 31 181 42 714.6 0 42 182 32 714.6 0 32 183 42 714.6 0 42 184 42 714.6 0 42 185 46 714.6 0 46 186 33 714.6 0 33 187 35 714.6 0 35 188 31 714.6 0 31 189 43 714.6 0 43 190 66 714.6 0 66 191 33 714.6 0 33 192 39 714.6 0 39 193 34 714.6 0 34 194 41 714.6 0 41 195 28 714.6 0 28 196 38 714.6 0 38 197 41 714.6 0 41 198 19 714.6 0 19 199 33 714.6 0 33 200 39 714.6 0 39 201 32 714.6 0 32 202 52 714.6 0 52 203 51 714.6 0 51 204 45 714.6 0 45 205 66 714.6 0 66 206 32 714.6 0 32 207 41 714.6 0 41 208 31 714.6 0 31 209 51 714.6 0 51 210 66 714.6 0 66 211 42 714.6 0 42 212 44 714.6 0 44 213 40 714.6 0 40 214 140 714.6 0 140 215 167 714.6 0 167 216 170 714.6 0 170 217 67 714.6 0 67 218 81 714.6 0 81 219 34 714.6 0 34 220 38 714.6 0 38 221 42 714.6 0 42 222 33 714.6 0 33 223 29 714.6 0 29 224 44 714.6 0 44 225 51 714.6 0 51 226 58 714.6 0 58 227 43 714.6 0 43 228 47 714.6 0 47 229 54 714.6 0 54 230 75 714.6 0 75 231 61 714.6 0 61 232 39 714.6 0 39 233 36 714.6 0 36 234 125 714.6 0 125 235 248 714.6 0 248 236 422 714.6 0 422 237 131 714.6 0 131 238 36 714.6 0 36 239 17 714.6 0 17 240 34 714.6 0 34 241 27 714.6 0 27 242 30 714.6 0 30 243 47 714.6 0 47 244 42 714.6 0 42 245 35 714.6 0 35 246 19 714.6 0 19 247 35 714.6 0 35 248 21 714.6 0 21 249 26 714.6 0 26 250 45 714.6 0 45 251 36 714.6 0 36 252 20 714.6 0 20 253 31 714.6 0 31 254 72 714.6 0 72 255 58 714.6 0 58 256 36 714.6 0 36 257 31 714.6 0 31 258 38 714.6 0 38 259 34 714.6 0 34 260 52 714.6 0 52 261 48 714.6 0 48 262 55 714.6 0 55 263 74 714.6 0 74 264 86 714.6 0 86 265 39 714.6 0 39 266 35 714.6 0 35 267 29 714.6 0 29 268 39 714.6 0 39 269 32 714.6 0 32 270 61 714.6 0 61 271 63 714.6 0 63 272 35 714.6 0 35 273 40 714.6 0 40 274 47 714.6 0 47 275 28 714.6 0 28 276 43 714.6 0 43 277 40 714.6 0 40 278 32 714.6 0 32 279 34 714.6 0 34 280 32 714.6 0 32 281 32 714.6 0 32 282 27 714.6 0 27 283 35 714.6 0 35 284 35 714.6 0 35 285 29 714.6 0 29 286 34 714.6 0 34 287 34 714.6 0 34 288 26 714.6 0 26 289 22 714.6 0 22 290 26 714.6 0 26 291 58 714.6 0 58 292 39 714.6 0 39 293 41 714.6 0 41 294 51 714.6 0 51 295 50 714.6 0 50 296 55 714.6 0 55 297 42 714.6 0 42 298 49 714.6 0 49 299 28 714.6 0 28 300 30 714.6 0 30 301 38 714.6 0 38 Finished in 1.45 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 31,238 Reads with adapters: 6,436 (20.6%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 6,433 (20.6%) Total basepairs processed: 7,252,039 bp Total written (filtered): 1,749,774 bp (24.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6436 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 12 0.0 3 0 0 0 0 12 18 7 0.0 3 0 0 2 0 5 19 49 0.0 3 0 44 5 20 5365 0.0 4 5285 77 3 21 775 0.0 4 762 12 1 22 6 0.0 4 0 5 1 23 205 0.0 4 201 3 0 1 25 2 0.0 4 1 0 0 0 1 97 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 1 1 112 1 0.0 4 0 0 0 1 127 6 0.0 4 0 0 0 0 6 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.22 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 6,433 Reads with adapters: 548 (8.5%) Reads written (passing filters): 6,433 (100.0%) Total basepairs processed: 1,749,774 bp Total written (filtered): 1,640,946 bp (93.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 548 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 71.9% C: 1.5% G: 25.7% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 8 1 0.1 0 1 9 8 0.0 0 0 8 10 108 0.0 1 1 107 11 35 0.0 1 0 35 257 1 0.0 1 0 1 259 1 0.0 1 1 260 2 0.0 1 2 268 1 0.0 1 1 270 1 0.0 1 1 271 372 0.0 1 358 14 272 17 0.0 1 17 273 1 0.0 1 1