This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.39 s (25 us/read; 2.43 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 72,863 (2.5%) Reads that were too short: 6,977 (0.2%) Reads written (passing filters): 65,886 (2.3%) Total basepairs processed: 881,011,047 bp Total written (filtered): 17,426,728 bp (2.0%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 72863 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 578 714.6 0 578 7 3074 714.6 0 3074 8 828 714.6 0 828 9 43349 714.6 0 43349 10 124 714.6 0 124 11 49 714.6 0 49 12 32 714.6 0 32 13 39 714.6 0 39 14 28 714.6 0 28 15 49 714.6 0 49 16 73 714.6 0 73 17 42 714.6 0 42 18 54 714.6 0 54 19 42 714.6 0 42 20 54 714.6 0 54 21 62 714.6 0 62 22 52 714.6 0 52 23 266 714.6 0 266 24 243 714.6 0 243 25 110 714.6 0 110 26 33 714.6 0 33 27 37 714.6 0 37 28 39 714.6 0 39 29 43 714.6 0 43 30 33 714.6 0 33 31 45 714.6 0 45 32 50 714.6 0 50 33 44 714.6 0 44 34 37 714.6 0 37 35 49 714.6 0 49 36 40 714.6 0 40 37 65 714.6 0 65 38 33 714.6 0 33 39 51 714.6 0 51 40 38 714.6 0 38 41 32 714.6 0 32 42 35 714.6 0 35 43 46 714.6 0 46 44 40 714.6 0 40 45 38 714.6 0 38 46 29 714.6 0 29 47 67 714.6 0 67 48 31 714.6 0 31 49 48 714.6 0 48 50 36 714.6 0 36 51 38 714.6 0 38 52 28 714.6 0 28 53 27 714.6 0 27 54 42 714.6 0 42 55 48 714.6 0 48 56 39 714.6 0 39 57 31 714.6 0 31 58 52 714.6 0 52 59 37 714.6 0 37 60 52 714.6 0 52 61 36 714.6 0 36 62 25 714.6 0 25 63 28 714.6 0 28 64 49 714.6 0 49 65 31 714.6 0 31 66 31 714.6 0 31 67 34 714.6 0 34 68 39 714.6 0 39 69 27 714.6 0 27 70 45 714.6 0 45 71 44 714.6 0 44 72 29 714.6 0 29 73 38 714.6 0 38 74 55 714.6 0 55 75 25 714.6 0 25 76 29 714.6 0 29 77 29 714.6 0 29 78 44 714.6 0 44 79 32 714.6 0 32 80 37 714.6 0 37 81 30 714.6 0 30 82 44 714.6 0 44 83 38 714.6 0 38 84 65 714.6 0 65 85 45 714.6 0 45 86 52 714.6 0 52 87 65 714.6 0 65 88 214 714.6 0 214 89 467 714.6 0 467 90 229 714.6 0 229 91 20 714.6 0 20 92 20 714.6 0 20 93 42 714.6 0 42 94 57 714.6 0 57 95 83 714.6 0 83 96 242 714.6 0 242 97 179 714.6 0 179 98 62 714.6 0 62 99 63 714.6 0 63 100 76 714.6 0 76 101 395 714.6 0 395 102 271 714.6 0 271 103 86 714.6 0 86 104 31 714.6 0 31 105 24 714.6 0 24 106 20 714.6 0 20 107 36 714.6 0 36 108 28 714.6 0 28 109 34 714.6 0 34 110 51 714.6 0 51 111 64 714.6 0 64 112 45 714.6 0 45 113 79 714.6 0 79 114 86 714.6 0 86 115 41 714.6 0 41 116 58 714.6 0 58 117 90 714.6 0 90 118 72 714.6 0 72 119 56 714.6 0 56 120 62 714.6 0 62 121 77 714.6 0 77 122 68 714.6 0 68 123 80 714.6 0 80 124 72 714.6 0 72 125 102 714.6 0 102 126 235 714.6 0 235 127 4933 714.6 0 4933 128 1559 714.6 0 1559 129 1180 714.6 0 1180 130 55 714.6 0 55 131 41 714.6 0 41 132 34 714.6 0 34 133 40 714.6 0 40 134 43 714.6 0 43 135 48 714.6 0 48 136 42 714.6 0 42 137 39 714.6 0 39 138 38 714.6 0 38 139 37 714.6 0 37 140 41 714.6 0 41 141 61 714.6 0 61 142 40 714.6 0 40 143 34 714.6 0 34 144 35 714.6 0 35 145 30 714.6 0 30 146 47 714.6 0 47 147 37 714.6 0 37 148 29 714.6 0 29 149 36 714.6 0 36 150 30 714.6 0 30 151 41 714.6 0 41 152 27 714.6 0 27 153 33 714.6 0 33 154 46 714.6 0 46 155 34 714.6 0 34 156 39 714.6 0 39 157 33 714.6 0 33 158 35 714.6 0 35 159 32 714.6 0 32 160 30 714.6 0 30 161 34 714.6 0 34 162 29 714.6 0 29 163 28 714.6 0 28 164 31 714.6 0 31 165 25 714.6 0 25 166 33 714.6 0 33 167 48 714.6 0 48 168 52 714.6 0 52 169 52 714.6 0 52 170 32 714.6 0 32 171 32 714.6 0 32 172 31 714.6 0 31 173 20 714.6 0 20 174 28 714.6 0 28 175 19 714.6 0 19 176 42 714.6 0 42 177 43 714.6 0 43 178 28 714.6 0 28 179 22 714.6 0 22 180 28 714.6 0 28 181 24 714.6 0 24 182 45 714.6 0 45 183 29 714.6 0 29 184 22 714.6 0 22 185 28 714.6 0 28 186 32 714.6 0 32 187 37 714.6 0 37 188 32 714.6 0 32 189 31 714.6 0 31 190 26 714.6 0 26 191 20 714.6 0 20 192 33 714.6 0 33 193 24 714.6 0 24 194 46 714.6 0 46 195 61 714.6 0 61 196 50 714.6 0 50 197 35 714.6 0 35 198 27 714.6 0 27 199 20 714.6 0 20 200 45 714.6 0 45 201 59 714.6 0 59 202 55 714.6 0 55 203 37 714.6 0 37 204 35 714.6 0 35 205 39 714.6 0 39 206 38 714.6 0 38 207 49 714.6 0 49 208 65 714.6 0 65 209 41 714.6 0 41 210 35 714.6 0 35 211 28 714.6 0 28 212 37 714.6 0 37 213 66 714.6 0 66 214 37 714.6 0 37 215 47 714.6 0 47 216 40 714.6 0 40 217 43 714.6 0 43 218 35 714.6 0 35 219 26 714.6 0 26 220 17 714.6 0 17 221 32 714.6 0 32 222 31 714.6 0 31 223 47 714.6 0 47 224 34 714.6 0 34 225 39 714.6 0 39 226 44 714.6 0 44 227 40 714.6 0 40 228 31 714.6 0 31 229 41 714.6 0 41 230 28 714.6 0 28 231 34 714.6 0 34 232 32 714.6 0 32 233 24 714.6 0 24 234 76 714.6 0 76 235 95 714.6 0 95 236 106 714.6 0 106 237 73 714.6 0 73 238 25 714.6 0 25 239 40 714.6 0 40 240 52 714.6 0 52 241 58 714.6 0 58 242 61 714.6 0 61 243 34 714.6 0 34 244 27 714.6 0 27 245 30 714.6 0 30 246 35 714.6 0 35 247 23 714.6 0 23 248 26 714.6 0 26 249 34 714.6 0 34 250 26 714.6 0 26 251 43 714.6 0 43 252 24 714.6 0 24 253 43 714.6 0 43 254 57 714.6 0 57 255 66 714.6 0 66 256 50 714.6 0 50 257 34 714.6 0 34 258 28 714.6 0 28 259 106 714.6 0 106 260 136 714.6 0 136 261 237 714.6 0 237 262 48 714.6 0 48 263 25 714.6 0 25 264 24 714.6 0 24 265 30 714.6 0 30 266 40 714.6 0 40 267 28 714.6 0 28 268 34 714.6 0 34 269 28 714.6 0 28 270 22 714.6 0 22 271 31 714.6 0 31 272 38 714.6 0 38 273 48 714.6 0 48 274 44 714.6 0 44 275 38 714.6 0 38 276 46 714.6 0 46 277 54 714.6 0 54 278 62 714.6 0 62 279 220 714.6 0 220 280 2533 714.6 0 2533 281 254 714.6 0 254 282 39 714.6 0 39 283 26 714.6 0 26 284 23 714.6 0 23 285 28 714.6 0 28 286 21 714.6 0 21 287 25 714.6 0 25 288 22 714.6 0 22 289 14 714.6 0 14 290 14 714.6 0 14 291 15 714.6 0 15 292 50 714.6 0 50 293 15 714.6 0 15 294 27 714.6 0 27 295 24 714.6 0 24 296 16 714.6 0 16 297 22 714.6 0 22 298 15 714.6 0 15 299 23 714.6 0 23 300 15 714.6 0 15 301 24 714.6 0 24 Finished in 2.97 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 65,886 Reads with adapters: 44,822 (68.0%) Reads that were too short: 33 (0.1%) Reads written (passing filters): 44,789 (68.0%) Total basepairs processed: 17,426,728 bp Total written (filtered): 12,297,063 bp (70.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 44822 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 14 0.0 3 0 0 11 3 16 428 0.0 3 0 401 22 5 17 44007 0.0 3 42546 1278 153 30 18 111 0.0 3 2 87 14 8 19 36 0.0 3 0 0 24 12 20 49 0.0 3 1 0 0 48 21 1 0.0 3 0 0 1 25 1 0.0 3 0 0 0 1 36 1 0.0 3 0 1 38 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 64 90 0.0 3 0 0 0 90 66 3 0.0 3 0 0 0 3 67 24 0.0 3 0 0 1 23 70 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 93 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 166 19 0.0 3 0 0 2 17 183 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.58 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 44,789 Reads with adapters: 1,446 (3.2%) Reads written (passing filters): 44,789 (100.0%) Total basepairs processed: 12,297,063 bp Total written (filtered): 12,292,699 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1446 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.2% C: 6.7% G: 41.2% T: 6.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1420 699.8 0 1420 4 26 175.0 0 26