This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.89 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 104,187 (3.6%) Reads that were too short: 24,681 (0.8%) Reads written (passing filters): 79,506 (2.7%) Total basepairs processed: 881,011,047 bp Total written (filtered): 20,305,003 bp (2.3%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 104187 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 408 714.6 0 408 7 11967 714.6 0 11967 8 43684 714.6 0 43684 9 83 714.6 0 83 10 18 714.6 0 18 11 14 714.6 0 14 12 29 714.6 0 29 13 24 714.6 0 24 14 22 714.6 0 22 15 21 714.6 0 21 16 28 714.6 0 28 17 22 714.6 0 22 18 29 714.6 0 29 19 31 714.6 0 31 20 49 714.6 0 49 21 76 714.6 0 76 22 62 714.6 0 62 23 16 714.6 0 16 24 17 714.6 0 17 25 19 714.6 0 19 26 17 714.6 0 17 27 36 714.6 0 36 28 19 714.6 0 19 29 18 714.6 0 18 30 17 714.6 0 17 31 14 714.6 0 14 32 22 714.6 0 22 33 28 714.6 0 28 34 22 714.6 0 22 35 21 714.6 0 21 36 29 714.6 0 29 37 36 714.6 0 36 38 23 714.6 0 23 39 24 714.6 0 24 40 21 714.6 0 21 41 27 714.6 0 27 42 23 714.6 0 23 43 11 714.6 0 11 44 29 714.6 0 29 45 29 714.6 0 29 46 40 714.6 0 40 47 32 714.6 0 32 48 28 714.6 0 28 49 34 714.6 0 34 50 33 714.6 0 33 51 30 714.6 0 30 52 61 714.6 0 61 53 25 714.6 0 25 54 45 714.6 0 45 55 59 714.6 0 59 56 113 714.6 0 113 57 19 714.6 0 19 58 24 714.6 0 24 59 24 714.6 0 24 60 30 714.6 0 30 61 44 714.6 0 44 62 46 714.6 0 46 63 25 714.6 0 25 64 19 714.6 0 19 65 33 714.6 0 33 66 28 714.6 0 28 67 87 714.6 0 87 68 50 714.6 0 50 69 25 714.6 0 25 70 17 714.6 0 17 71 33 714.6 0 33 72 39 714.6 0 39 73 25 714.6 0 25 74 29 714.6 0 29 75 25 714.6 0 25 76 47 714.6 0 47 77 99 714.6 0 99 78 151 714.6 0 151 79 66 714.6 0 66 80 53 714.6 0 53 81 54 714.6 0 54 82 25 714.6 0 25 83 70 714.6 0 70 84 27 714.6 0 27 85 20 714.6 0 20 86 32 714.6 0 32 87 174 714.6 0 174 88 27 714.6 0 27 89 36 714.6 0 36 90 54 714.6 0 54 91 52 714.6 0 52 92 98 714.6 0 98 93 282 714.6 0 282 94 270 714.6 0 270 95 162 714.6 0 162 96 115 714.6 0 115 97 104 714.6 0 104 98 300 714.6 0 300 99 656 714.6 0 656 100 1029 714.6 0 1029 101 111 714.6 0 111 102 41 714.6 0 41 103 27 714.6 0 27 104 17 714.6 0 17 105 20 714.6 0 20 106 31 714.6 0 31 107 29 714.6 0 29 108 58 714.6 0 58 109 79 714.6 0 79 110 96 714.6 0 96 111 144 714.6 0 144 112 89 714.6 0 89 113 186 714.6 0 186 114 86 714.6 0 86 115 85 714.6 0 85 116 91 714.6 0 91 117 54 714.6 0 54 118 78 714.6 0 78 119 73 714.6 0 73 120 102 714.6 0 102 121 103 714.6 0 103 122 109 714.6 0 109 123 141 714.6 0 141 124 139 714.6 0 139 125 175 714.6 0 175 126 194 714.6 0 194 127 721 714.6 0 721 128 581 714.6 0 581 129 387 714.6 0 387 130 561 714.6 0 561 131 286 714.6 0 286 132 127 714.6 0 127 133 119 714.6 0 119 134 147 714.6 0 147 135 220 714.6 0 220 136 202 714.6 0 202 137 179 714.6 0 179 138 109 714.6 0 109 139 121 714.6 0 121 140 114 714.6 0 114 141 120 714.6 0 120 142 130 714.6 0 130 143 117 714.6 0 117 144 208 714.6 0 208 145 115 714.6 0 115 146 158 714.6 0 158 147 126 714.6 0 126 148 227 714.6 0 227 149 122 714.6 0 122 150 105 714.6 0 105 151 73 714.6 0 73 152 111 714.6 0 111 153 133 714.6 0 133 154 139 714.6 0 139 155 173 714.6 0 173 156 133 714.6 0 133 157 154 714.6 0 154 158 117 714.6 0 117 159 129 714.6 0 129 160 155 714.6 0 155 161 110 714.6 0 110 162 128 714.6 0 128 163 128 714.6 0 128 164 127 714.6 0 127 165 191 714.6 0 191 166 370 714.6 0 370 167 312 714.6 0 312 168 196 714.6 0 196 169 131 714.6 0 131 170 97 714.6 0 97 171 124 714.6 0 124 172 116 714.6 0 116 173 132 714.6 0 132 174 202 714.6 0 202 175 184 714.6 0 184 176 145 714.6 0 145 177 120 714.6 0 120 178 162 714.6 0 162 179 168 714.6 0 168 180 184 714.6 0 184 181 167 714.6 0 167 182 181 714.6 0 181 183 211 714.6 0 211 184 178 714.6 0 178 185 213 714.6 0 213 186 180 714.6 0 180 187 138 714.6 0 138 188 133 714.6 0 133 189 130 714.6 0 130 190 220 714.6 0 220 191 284 714.6 0 284 192 330 714.6 0 330 193 257 714.6 0 257 194 178 714.6 0 178 195 283 714.6 0 283 196 415 714.6 0 415 197 457 714.6 0 457 198 562 714.6 0 562 199 135 714.6 0 135 200 222 714.6 0 222 201 118 714.6 0 118 202 282 714.6 0 282 203 315 714.6 0 315 204 254 714.6 0 254 205 216 714.6 0 216 206 207 714.6 0 207 207 159 714.6 0 159 208 162 714.6 0 162 209 189 714.6 0 189 210 334 714.6 0 334 211 435 714.6 0 435 212 442 714.6 0 442 213 354 714.6 0 354 214 215 714.6 0 215 215 221 714.6 0 221 216 237 714.6 0 237 217 197 714.6 0 197 218 259 714.6 0 259 219 359 714.6 0 359 220 295 714.6 0 295 221 257 714.6 0 257 222 280 714.6 0 280 223 310 714.6 0 310 224 244 714.6 0 244 225 234 714.6 0 234 226 221 714.6 0 221 227 186 714.6 0 186 228 150 714.6 0 150 229 133 714.6 0 133 230 134 714.6 0 134 231 148 714.6 0 148 232 180 714.6 0 180 233 259 714.6 0 259 234 303 714.6 0 303 235 320 714.6 0 320 236 309 714.6 0 309 237 323 714.6 0 323 238 372 714.6 0 372 239 368 714.6 0 368 240 457 714.6 0 457 241 278 714.6 0 278 242 459 714.6 0 459 243 276 714.6 0 276 244 321 714.6 0 321 245 515 714.6 0 515 246 533 714.6 0 533 247 338 714.6 0 338 248 39 714.6 0 39 249 73 714.6 0 73 250 127 714.6 0 127 251 502 714.6 0 502 252 562 714.6 0 562 253 391 714.6 0 391 254 282 714.6 0 282 255 374 714.6 0 374 256 227 714.6 0 227 257 164 714.6 0 164 258 110 714.6 0 110 259 143 714.6 0 143 260 135 714.6 0 135 261 215 714.6 0 215 262 310 714.6 0 310 263 501 714.6 0 501 264 424 714.6 0 424 265 267 714.6 0 267 266 157 714.6 0 157 267 96 714.6 0 96 268 89 714.6 0 89 269 77 714.6 0 77 270 122 714.6 0 122 271 263 714.6 0 263 272 356 714.6 0 356 273 407 714.6 0 407 274 244 714.6 0 244 275 148 714.6 0 148 276 158 714.6 0 158 277 150 714.6 0 150 278 238 714.6 0 238 279 201 714.6 0 201 280 127 714.6 0 127 281 109 714.6 0 109 282 174 714.6 0 174 283 310 714.6 0 310 284 269 714.6 0 269 285 226 714.6 0 226 286 138 714.6 0 138 287 84 714.6 0 84 288 52 714.6 0 52 289 76 714.6 0 76 290 238 714.6 0 238 291 399 714.6 0 399 292 268 714.6 0 268 293 105 714.6 0 105 294 60 714.6 0 60 295 49 714.6 0 49 296 55 714.6 0 55 297 140 714.6 0 140 298 211 714.6 0 211 299 420 714.6 0 420 300 451 714.6 0 451 301 128 714.6 0 128 Finished in 3.68 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 79,506 Reads with adapters: 45,201 (56.9%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 45,189 (56.8%) Total basepairs processed: 20,305,003 bp Total written (filtered): 12,337,207 bp (60.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 45201 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 14 0.0 3 0 0 0 1 13 18 13 0.0 3 0 0 13 19 491 0.0 3 0 483 5 0 3 20 44514 0.0 4 44000 502 9 2 1 21 108 0.0 4 19 89 22 15 0.0 4 0 0 12 1 2 23 22 0.0 4 0 0 0 20 2 24 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 1 32 3 0.0 4 0 0 0 0 3 44 1 0.0 4 1 52 1 0.0 4 0 0 0 0 1 58 3 0.0 4 0 0 0 0 3 89 1 0.0 4 0 0 0 0 1 96 2 0.0 4 0 0 0 0 2 101 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 1 123 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.54 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 45,189 Reads with adapters: 2,193 (4.9%) Reads written (passing filters): 45,189 (100.0%) Total basepairs processed: 12,337,207 bp Total written (filtered): 12,310,023 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2193 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 1.2% G: 97.3% T: 0.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 706.1 0 1 4 9 176.5 0 9 5 1 44.1 0 1 9 1 0.2 0 0 1 10 134 0.0 1 6 128 11 2023 0.0 1 14 2009 12 8 0.0 1 0 8 72 1 0.0 1 0 1 151 3 0.0 1 0 3 153 1 0.0 1 0 1 170 1 0.0 1 1 224 1 0.0 1 1 259 4 0.0 1 4 260 1 0.0 1 1 261 1 0.0 1 1 271 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.