This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.40 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 112,223 (3.8%) Reads that were too short: 26,323 (0.9%) Reads written (passing filters): 85,900 (2.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 19,291,912 bp (2.2%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 112223 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 195 714.6 0 195 7 662 714.6 0 662 8 34569 714.6 0 34569 9 68 714.6 0 68 10 13 714.6 0 13 11 24 714.6 0 24 12 14 714.6 0 14 13 12 714.6 0 12 14 30 714.6 0 30 15 25 714.6 0 25 16 28 714.6 0 28 17 17 714.6 0 17 18 29 714.6 0 29 19 21 714.6 0 21 20 18 714.6 0 18 21 26 714.6 0 26 22 17 714.6 0 17 23 22 714.6 0 22 24 19 714.6 0 19 25 19 714.6 0 19 26 27 714.6 0 27 27 21 714.6 0 21 28 52 714.6 0 52 29 112 714.6 0 112 30 67 714.6 0 67 31 24 714.6 0 24 32 19 714.6 0 19 33 16 714.6 0 16 34 22 714.6 0 22 35 19 714.6 0 19 36 25 714.6 0 25 37 22 714.6 0 22 38 12 714.6 0 12 39 28 714.6 0 28 40 93 714.6 0 93 41 18 714.6 0 18 42 16 714.6 0 16 43 18 714.6 0 18 44 17 714.6 0 17 45 82 714.6 0 82 46 79 714.6 0 79 47 73 714.6 0 73 48 19 714.6 0 19 49 27 714.6 0 27 50 11 714.6 0 11 51 14 714.6 0 14 52 13 714.6 0 13 53 11 714.6 0 11 54 15 714.6 0 15 55 12 714.6 0 12 56 30 714.6 0 30 57 16 714.6 0 16 58 17 714.6 0 17 59 15 714.6 0 15 60 18 714.6 0 18 61 15 714.6 0 15 62 16 714.6 0 16 63 10 714.6 0 10 64 20 714.6 0 20 65 19 714.6 0 19 66 14 714.6 0 14 67 9 714.6 0 9 68 17 714.6 0 17 69 18 714.6 0 18 70 58 714.6 0 58 71 234 714.6 0 234 72 258 714.6 0 258 73 96 714.6 0 96 74 183 714.6 0 183 75 229 714.6 0 229 76 391 714.6 0 391 77 939 714.6 0 939 78 473 714.6 0 473 79 472 714.6 0 472 80 847 714.6 0 847 81 543 714.6 0 543 82 571 714.6 0 571 83 1085 714.6 0 1085 84 1106 714.6 0 1106 85 324 714.6 0 324 86 219 714.6 0 219 87 143 714.6 0 143 88 132 714.6 0 132 89 397 714.6 0 397 90 638 714.6 0 638 91 978 714.6 0 978 92 1569 714.6 0 1569 93 848 714.6 0 848 94 264 714.6 0 264 95 717 714.6 0 717 96 886 714.6 0 886 97 533 714.6 0 533 98 417 714.6 0 417 99 296 714.6 0 296 100 320 714.6 0 320 101 180 714.6 0 180 102 71 714.6 0 71 103 43 714.6 0 43 104 35 714.6 0 35 105 58 714.6 0 58 106 111 714.6 0 111 107 193 714.6 0 193 108 287 714.6 0 287 109 321 714.6 0 321 110 324 714.6 0 324 111 289 714.6 0 289 112 264 714.6 0 264 113 984 714.6 0 984 114 1258 714.6 0 1258 115 1121 714.6 0 1121 116 676 714.6 0 676 117 329 714.6 0 329 118 314 714.6 0 314 119 313 714.6 0 313 120 351 714.6 0 351 121 279 714.6 0 279 122 369 714.6 0 369 123 390 714.6 0 390 124 280 714.6 0 280 125 306 714.6 0 306 126 385 714.6 0 385 127 265 714.6 0 265 128 470 714.6 0 470 129 625 714.6 0 625 130 526 714.6 0 526 131 465 714.6 0 465 132 338 714.6 0 338 133 263 714.6 0 263 134 352 714.6 0 352 135 531 714.6 0 531 136 760 714.6 0 760 137 484 714.6 0 484 138 413 714.6 0 413 139 608 714.6 0 608 140 330 714.6 0 330 141 263 714.6 0 263 142 323 714.6 0 323 143 393 714.6 0 393 144 326 714.6 0 326 145 251 714.6 0 251 146 205 714.6 0 205 147 222 714.6 0 222 148 292 714.6 0 292 149 301 714.6 0 301 150 254 714.6 0 254 151 153 714.6 0 153 152 300 714.6 0 300 153 344 714.6 0 344 154 279 714.6 0 279 155 321 714.6 0 321 156 269 714.6 0 269 157 262 714.6 0 262 158 252 714.6 0 252 159 181 714.6 0 181 160 159 714.6 0 159 161 177 714.6 0 177 162 253 714.6 0 253 163 319 714.6 0 319 164 409 714.6 0 409 165 273 714.6 0 273 166 253 714.6 0 253 167 278 714.6 0 278 168 321 714.6 0 321 169 298 714.6 0 298 170 283 714.6 0 283 171 238 714.6 0 238 172 220 714.6 0 220 173 254 714.6 0 254 174 247 714.6 0 247 175 289 714.6 0 289 176 189 714.6 0 189 177 305 714.6 0 305 178 370 714.6 0 370 179 273 714.6 0 273 180 274 714.6 0 274 181 255 714.6 0 255 182 266 714.6 0 266 183 295 714.6 0 295 184 341 714.6 0 341 185 310 714.6 0 310 186 181 714.6 0 181 187 193 714.6 0 193 188 291 714.6 0 291 189 344 714.6 0 344 190 458 714.6 0 458 191 390 714.6 0 390 192 327 714.6 0 327 193 206 714.6 0 206 194 176 714.6 0 176 195 293 714.6 0 293 196 256 714.6 0 256 197 389 714.6 0 389 198 254 714.6 0 254 199 159 714.6 0 159 200 243 714.6 0 243 201 227 714.6 0 227 202 333 714.6 0 333 203 693 714.6 0 693 204 506 714.6 0 506 205 329 714.6 0 329 206 245 714.6 0 245 207 267 714.6 0 267 208 313 714.6 0 313 209 406 714.6 0 406 210 484 714.6 0 484 211 425 714.6 0 425 212 328 714.6 0 328 213 232 714.6 0 232 214 237 714.6 0 237 215 370 714.6 0 370 216 376 714.6 0 376 217 325 714.6 0 325 218 195 714.6 0 195 219 183 714.6 0 183 220 191 714.6 0 191 221 267 714.6 0 267 222 220 714.6 0 220 223 264 714.6 0 264 224 288 714.6 0 288 225 860 714.6 0 860 226 751 714.6 0 751 227 1092 714.6 0 1092 228 220 714.6 0 220 229 154 714.6 0 154 230 159 714.6 0 159 231 238 714.6 0 238 232 269 714.6 0 269 233 298 714.6 0 298 234 334 714.6 0 334 235 383 714.6 0 383 236 445 714.6 0 445 237 458 714.6 0 458 238 145 714.6 0 145 239 155 714.6 0 155 240 168 714.6 0 168 241 220 714.6 0 220 242 267 714.6 0 267 243 348 714.6 0 348 244 252 714.6 0 252 245 188 714.6 0 188 246 146 714.6 0 146 247 94 714.6 0 94 248 86 714.6 0 86 249 156 714.6 0 156 250 255 714.6 0 255 251 218 714.6 0 218 252 426 714.6 0 426 253 322 714.6 0 322 254 184 714.6 0 184 255 153 714.6 0 153 256 88 714.6 0 88 257 81 714.6 0 81 258 100 714.6 0 100 259 167 714.6 0 167 260 203 714.6 0 203 261 259 714.6 0 259 262 246 714.6 0 246 263 360 714.6 0 360 264 342 714.6 0 342 265 231 714.6 0 231 266 116 714.6 0 116 267 76 714.6 0 76 268 82 714.6 0 82 269 175 714.6 0 175 270 319 714.6 0 319 271 265 714.6 0 265 272 259 714.6 0 259 273 140 714.6 0 140 274 120 714.6 0 120 275 180 714.6 0 180 276 159 714.6 0 159 277 197 714.6 0 197 278 217 714.6 0 217 279 154 714.6 0 154 280 92 714.6 0 92 281 120 714.6 0 120 282 157 714.6 0 157 283 183 714.6 0 183 284 223 714.6 0 223 285 149 714.6 0 149 286 124 714.6 0 124 287 134 714.6 0 134 288 115 714.6 0 115 289 352 714.6 0 352 290 382 714.6 0 382 291 328 714.6 0 328 292 192 714.6 0 192 293 178 714.6 0 178 294 163 714.6 0 163 295 313 714.6 0 313 296 395 714.6 0 395 297 415 714.6 0 415 298 264 714.6 0 264 299 229 714.6 0 229 300 223 714.6 0 223 301 135 714.6 0 135 Finished in 3.41 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 85,900 Reads with adapters: 35,261 (41.0%) Reads that were too short: 27 (0.0%) Reads written (passing filters): 35,234 (41.0%) Total basepairs processed: 19,291,912 bp Total written (filtered): 9,719,510 bp (50.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 35261 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 15 0.0 3 0 0 9 6 16 280 0.0 3 0 244 23 13 17 34682 0.0 3 33383 1103 154 42 18 110 0.0 3 17 71 14 8 19 73 0.0 3 1 0 37 35 20 25 0.0 3 0 0 0 25 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 38 5 0.0 3 0 0 3 2 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 52 4 0.0 3 0 0 0 4 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 58 7 0.0 3 0 0 0 7 61 1 0.0 3 0 0 1 65 1 0.0 3 0 0 1 68 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 78 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 156 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 200 2 0.0 3 0 0 0 2 253 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.23 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 35,234 Reads with adapters: 714 (2.0%) Reads written (passing filters): 35,234 (100.0%) Total basepairs processed: 9,719,510 bp Total written (filtered): 9,717,342 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 714 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 41.3% C: 10.1% G: 41.0% T: 7.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 690 550.5 0 690 4 22 137.6 0 22 5 2 34.4 0 2