This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.52 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 185,649 (6.3%) Reads that were too short: 81,669 (2.8%) Reads written (passing filters): 103,980 (3.6%) Total basepairs processed: 881,011,047 bp Total written (filtered): 23,205,242 bp (2.6%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 185649 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2154 714.6 0 2154 7 34828 714.6 0 34828 8 385 714.6 0 385 9 14328 714.6 0 14328 10 25 714.6 0 25 11 12 714.6 0 12 12 15 714.6 0 15 13 15 714.6 0 15 14 11 714.6 0 11 15 1129 714.6 0 1129 16 171 714.6 0 171 17 7 714.6 0 7 18 17 714.6 0 17 19 5 714.6 0 5 20 17 714.6 0 17 21 13 714.6 0 13 22 8 714.6 0 8 23 21 714.6 0 21 24 13 714.6 0 13 25 18 714.6 0 18 26 18 714.6 0 18 27 9 714.6 0 9 28 16 714.6 0 16 29 18 714.6 0 18 30 15 714.6 0 15 31 11 714.6 0 11 32 14 714.6 0 14 33 14 714.6 0 14 34 13 714.6 0 13 35 26 714.6 0 26 36 12 714.6 0 12 37 13 714.6 0 13 38 40 714.6 0 40 39 30 714.6 0 30 40 153 714.6 0 153 41 30 714.6 0 30 42 19 714.6 0 19 43 17 714.6 0 17 44 11 714.6 0 11 45 19 714.6 0 19 46 12 714.6 0 12 47 20 714.6 0 20 48 10 714.6 0 10 49 16 714.6 0 16 50 14 714.6 0 14 51 23 714.6 0 23 52 17 714.6 0 17 53 14 714.6 0 14 54 9 714.6 0 9 55 9 714.6 0 9 56 12 714.6 0 12 57 15 714.6 0 15 58 18 714.6 0 18 59 17 714.6 0 17 60 7 714.6 0 7 61 27 714.6 0 27 62 5 714.6 0 5 63 14 714.6 0 14 64 10 714.6 0 10 65 49 714.6 0 49 66 29 714.6 0 29 67 36 714.6 0 36 68 29 714.6 0 29 69 191 714.6 0 191 70 1045 714.6 0 1045 71 288 714.6 0 288 72 10 714.6 0 10 73 16 714.6 0 16 74 14 714.6 0 14 75 13 714.6 0 13 76 9 714.6 0 9 77 16 714.6 0 16 78 10 714.6 0 10 79 26 714.6 0 26 80 42 714.6 0 42 81 169 714.6 0 169 82 95 714.6 0 95 83 62 714.6 0 62 84 72 714.6 0 72 85 88 714.6 0 88 86 68 714.6 0 68 87 50 714.6 0 50 88 55 714.6 0 55 89 109 714.6 0 109 90 47 714.6 0 47 91 66 714.6 0 66 92 33 714.6 0 33 93 61 714.6 0 61 94 75 714.6 0 75 95 72 714.6 0 72 96 86 714.6 0 86 97 111 714.6 0 111 98 77 714.6 0 77 99 89 714.6 0 89 100 398 714.6 0 398 101 590 714.6 0 590 102 1267 714.6 0 1267 103 689 714.6 0 689 104 304 714.6 0 304 105 131 714.6 0 131 106 50 714.6 0 50 107 61 714.6 0 61 108 22 714.6 0 22 109 34 714.6 0 34 110 66 714.6 0 66 111 124 714.6 0 124 112 129 714.6 0 129 113 115 714.6 0 115 114 121 714.6 0 121 115 141 714.6 0 141 116 145 714.6 0 145 117 245 714.6 0 245 118 346 714.6 0 346 119 248 714.6 0 248 120 227 714.6 0 227 121 183 714.6 0 183 122 229 714.6 0 229 123 510 714.6 0 510 124 540 714.6 0 540 125 257 714.6 0 257 126 167 714.6 0 167 127 192 714.6 0 192 128 177 714.6 0 177 129 141 714.6 0 141 130 250 714.6 0 250 131 410 714.6 0 410 132 775 714.6 0 775 133 578 714.6 0 578 134 342 714.6 0 342 135 242 714.6 0 242 136 199 714.6 0 199 137 359 714.6 0 359 138 403 714.6 0 403 139 418 714.6 0 418 140 337 714.6 0 337 141 303 714.6 0 303 142 670 714.6 0 670 143 892 714.6 0 892 144 963 714.6 0 963 145 499 714.6 0 499 146 333 714.6 0 333 147 379 714.6 0 379 148 500 714.6 0 500 149 466 714.6 0 466 150 429 714.6 0 429 151 480 714.6 0 480 152 340 714.6 0 340 153 321 714.6 0 321 154 333 714.6 0 333 155 310 714.6 0 310 156 443 714.6 0 443 157 528 714.6 0 528 158 769 714.6 0 769 159 827 714.6 0 827 160 1220 714.6 0 1220 161 872 714.6 0 872 162 771 714.6 0 771 163 488 714.6 0 488 164 381 714.6 0 381 165 292 714.6 0 292 166 268 714.6 0 268 167 381 714.6 0 381 168 712 714.6 0 712 169 769 714.6 0 769 170 598 714.6 0 598 171 486 714.6 0 486 172 425 714.6 0 425 173 333 714.6 0 333 174 275 714.6 0 275 175 302 714.6 0 302 176 1327 714.6 0 1327 177 1390 714.6 0 1390 178 774 714.6 0 774 179 545 714.6 0 545 180 389 714.6 0 389 181 356 714.6 0 356 182 474 714.6 0 474 183 519 714.6 0 519 184 592 714.6 0 592 185 558 714.6 0 558 186 514 714.6 0 514 187 734 714.6 0 734 188 832 714.6 0 832 189 1199 714.6 0 1199 190 632 714.6 0 632 191 368 714.6 0 368 192 263 714.6 0 263 193 255 714.6 0 255 194 497 714.6 0 497 195 521 714.6 0 521 196 769 714.6 0 769 197 591 714.6 0 591 198 394 714.6 0 394 199 616 714.6 0 616 200 508 714.6 0 508 201 576 714.6 0 576 202 425 714.6 0 425 203 284 714.6 0 284 204 490 714.6 0 490 205 542 714.6 0 542 206 515 714.6 0 515 207 487 714.6 0 487 208 461 714.6 0 461 209 529 714.6 0 529 210 736 714.6 0 736 211 880 714.6 0 880 212 743 714.6 0 743 213 693 714.6 0 693 214 794 714.6 0 794 215 752 714.6 0 752 216 753 714.6 0 753 217 716 714.6 0 716 218 668 714.6 0 668 219 598 714.6 0 598 220 667 714.6 0 667 221 690 714.6 0 690 222 832 714.6 0 832 223 870 714.6 0 870 224 978 714.6 0 978 225 1066 714.6 0 1066 226 727 714.6 0 727 227 735 714.6 0 735 228 726 714.6 0 726 229 682 714.6 0 682 230 933 714.6 0 933 231 602 714.6 0 602 232 544 714.6 0 544 233 619 714.6 0 619 234 681 714.6 0 681 235 614 714.6 0 614 236 698 714.6 0 698 237 669 714.6 0 669 238 880 714.6 0 880 239 1027 714.6 0 1027 240 1393 714.6 0 1393 241 956 714.6 0 956 242 719 714.6 0 719 243 777 714.6 0 777 244 868 714.6 0 868 245 996 714.6 0 996 246 885 714.6 0 885 247 1091 714.6 0 1091 248 950 714.6 0 950 249 1308 714.6 0 1308 250 655 714.6 0 655 251 541 714.6 0 541 252 298 714.6 0 298 253 588 714.6 0 588 254 910 714.6 0 910 255 1064 714.6 0 1064 256 1083 714.6 0 1083 257 970 714.6 0 970 258 881 714.6 0 881 259 858 714.6 0 858 260 826 714.6 0 826 261 647 714.6 0 647 262 679 714.6 0 679 263 1054 714.6 0 1054 264 1656 714.6 0 1656 265 1373 714.6 0 1373 266 977 714.6 0 977 267 829 714.6 0 829 268 662 714.6 0 662 269 1057 714.6 0 1057 270 676 714.6 0 676 271 648 714.6 0 648 272 599 714.6 0 599 273 848 714.6 0 848 274 1226 714.6 0 1226 275 1205 714.6 0 1205 276 1250 714.6 0 1250 277 1184 714.6 0 1184 278 1001 714.6 0 1001 279 680 714.6 0 680 280 1021 714.6 0 1021 281 991 714.6 0 991 282 1341 714.6 0 1341 283 889 714.6 0 889 284 846 714.6 0 846 285 1071 714.6 0 1071 286 902 714.6 0 902 287 1053 714.6 0 1053 288 1129 714.6 0 1129 289 986 714.6 0 986 290 631 714.6 0 631 291 495 714.6 0 495 292 551 714.6 0 551 293 850 714.6 0 850 294 888 714.6 0 888 295 937 714.6 0 937 296 653 714.6 0 653 297 498 714.6 0 498 298 608 714.6 0 608 299 762 714.6 0 762 300 909 714.6 0 909 301 484 714.6 0 484 Finished in 4.49 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 103,980 Reads with adapters: 36,687 (35.3%) Reads that were too short: 26 (0.0%) Reads written (passing filters): 36,661 (35.3%) Total basepairs processed: 23,205,242 bp Total written (filtered): 10,045,886 bp (43.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 36687 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 0 4 18 10 0.0 3 0 0 10 19 308 0.0 3 0 301 5 1 1 20 36195 0.0 4 35706 473 13 2 1 21 75 0.0 4 8 66 1 22 6 0.0 4 1 1 4 23 56 0.0 4 38 2 0 16 39 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 57 2 0.0 4 0 0 0 0 2 63 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 95 2 0.0 4 0 0 0 0 2 100 2 0.0 4 0 0 0 0 2 102 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 200 1 0.0 4 1 228 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.23 s (34 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 36,661 Reads with adapters: 613 (1.7%) Reads written (passing filters): 36,661 (100.0%) Total basepairs processed: 10,045,886 bp Total written (filtered): 10,038,198 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 613 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 2.3% G: 94.5% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 15 572.8 0 15 4 2 143.2 0 2 5 1 35.8 0 1 10 8 0.0 1 0 8 11 557 0.0 1 1 556 12 23 0.0 1 0 23 13 1 0.0 1 0 1 152 3 0.0 1 0 3 153 1 0.0 1 0 1 262 1 0.0 1 1 263 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.