This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.38 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 785,124 (26.8%) Reads that were too short: 145,865 (5.0%) Reads written (passing filters): 639,259 (21.8%) Total basepairs processed: 881,011,047 bp Total written (filtered): 112,712,525 bp (12.8%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 785124 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 368 714.6 0 368 7 13509 714.6 0 13509 8 11115 714.6 0 11115 9 380 714.6 0 380 10 211 714.6 0 211 11 67 714.6 0 67 12 63 714.6 0 63 13 64 714.6 0 64 14 57 714.6 0 57 15 63 714.6 0 63 16 55 714.6 0 55 17 54 714.6 0 54 18 51 714.6 0 51 19 57 714.6 0 57 20 65 714.6 0 65 21 38 714.6 0 38 22 52 714.6 0 52 23 51 714.6 0 51 24 61 714.6 0 61 25 83 714.6 0 83 26 88 714.6 0 88 27 134 714.6 0 134 28 74 714.6 0 74 29 70 714.6 0 70 30 71 714.6 0 71 31 48 714.6 0 48 32 52 714.6 0 52 33 44 714.6 0 44 34 107 714.6 0 107 35 73 714.6 0 73 36 55 714.6 0 55 37 95 714.6 0 95 38 51 714.6 0 51 39 41 714.6 0 41 40 82 714.6 0 82 41 61 714.6 0 61 42 55 714.6 0 55 43 82 714.6 0 82 44 64 714.6 0 64 45 54 714.6 0 54 46 56 714.6 0 56 47 60 714.6 0 60 48 51 714.6 0 51 49 42 714.6 0 42 50 68 714.6 0 68 51 50 714.6 0 50 52 59 714.6 0 59 53 69 714.6 0 69 54 81 714.6 0 81 55 50 714.6 0 50 56 73 714.6 0 73 57 64 714.6 0 64 58 45 714.6 0 45 59 106 714.6 0 106 60 68 714.6 0 68 61 80 714.6 0 80 62 75 714.6 0 75 63 83 714.6 0 83 64 82 714.6 0 82 65 82 714.6 0 82 66 93 714.6 0 93 67 117 714.6 0 117 68 145 714.6 0 145 69 158 714.6 0 158 70 193 714.6 0 193 71 306 714.6 0 306 72 2021 714.6 0 2021 73 1368 714.6 0 1368 74 2884 714.6 0 2884 75 5421 714.6 0 5421 76 2267 714.6 0 2267 77 3491 714.6 0 3491 78 4470 714.6 0 4470 79 7050 714.6 0 7050 80 5889 714.6 0 5889 81 8997 714.6 0 8997 82 6343 714.6 0 6343 83 8876 714.6 0 8876 84 11735 714.6 0 11735 85 9329 714.6 0 9329 86 5974 714.6 0 5974 87 7289 714.6 0 7289 88 3398 714.6 0 3398 89 2536 714.6 0 2536 90 6139 714.6 0 6139 91 2242 714.6 0 2242 92 3043 714.6 0 3043 93 5490 714.6 0 5490 94 3695 714.6 0 3695 95 3291 714.6 0 3291 96 3791 714.6 0 3791 97 2699 714.6 0 2699 98 3915 714.6 0 3915 99 3067 714.6 0 3067 100 2010 714.6 0 2010 101 3350 714.6 0 3350 102 2561 714.6 0 2561 103 1830 714.6 0 1830 104 1467 714.6 0 1467 105 1866 714.6 0 1866 106 1654 714.6 0 1654 107 1392 714.6 0 1392 108 1998 714.6 0 1998 109 2122 714.6 0 2122 110 2643 714.6 0 2643 111 3060 714.6 0 3060 112 2718 714.6 0 2718 113 3384 714.6 0 3384 114 3752 714.6 0 3752 115 3825 714.6 0 3825 116 4010 714.6 0 4010 117 5125 714.6 0 5125 118 12553 714.6 0 12553 119 40185 714.6 0 40185 120 17584 714.6 0 17584 121 4606 714.6 0 4606 122 5888 714.6 0 5888 123 4930 714.6 0 4930 124 2781 714.6 0 2781 125 2434 714.6 0 2434 126 3082 714.6 0 3082 127 3094 714.6 0 3094 128 2407 714.6 0 2407 129 3232 714.6 0 3232 130 3046 714.6 0 3046 131 2793 714.6 0 2793 132 3689 714.6 0 3689 133 3038 714.6 0 3038 134 3214 714.6 0 3214 135 3285 714.6 0 3285 136 3109 714.6 0 3109 137 3438 714.6 0 3438 138 3466 714.6 0 3466 139 3919 714.6 0 3919 140 13836 714.6 0 13836 141 10573 714.6 0 10573 142 10214 714.6 0 10214 143 4693 714.6 0 4693 144 4507 714.6 0 4507 145 10323 714.6 0 10323 146 38284 714.6 0 38284 147 23748 714.6 0 23748 148 7456 714.6 0 7456 149 34460 714.6 0 34460 150 5512 714.6 0 5512 151 3144 714.6 0 3144 152 4680 714.6 0 4680 153 4200 714.6 0 4200 154 2351 714.6 0 2351 155 2781 714.6 0 2781 156 2404 714.6 0 2404 157 2048 714.6 0 2048 158 2401 714.6 0 2401 159 1928 714.6 0 1928 160 1999 714.6 0 1999 161 1741 714.6 0 1741 162 2093 714.6 0 2093 163 1860 714.6 0 1860 164 2120 714.6 0 2120 165 3524 714.6 0 3524 166 6990 714.6 0 6990 167 6692 714.6 0 6692 168 2317 714.6 0 2317 169 2238 714.6 0 2238 170 2025 714.6 0 2025 171 1914 714.6 0 1914 172 2294 714.6 0 2294 173 2333 714.6 0 2333 174 1951 714.6 0 1951 175 2308 714.6 0 2308 176 2168 714.6 0 2168 177 1737 714.6 0 1737 178 2078 714.6 0 2078 179 1661 714.6 0 1661 180 1694 714.6 0 1694 181 1962 714.6 0 1962 182 1754 714.6 0 1754 183 1782 714.6 0 1782 184 2002 714.6 0 2002 185 2211 714.6 0 2211 186 2094 714.6 0 2094 187 1873 714.6 0 1873 188 2249 714.6 0 2249 189 2027 714.6 0 2027 190 1832 714.6 0 1832 191 1995 714.6 0 1995 192 2365 714.6 0 2365 193 2882 714.6 0 2882 194 1969 714.6 0 1969 195 2617 714.6 0 2617 196 2801 714.6 0 2801 197 1673 714.6 0 1673 198 2394 714.6 0 2394 199 1683 714.6 0 1683 200 2041 714.6 0 2041 201 2302 714.6 0 2302 202 1649 714.6 0 1649 203 1753 714.6 0 1753 204 1689 714.6 0 1689 205 1735 714.6 0 1735 206 1667 714.6 0 1667 207 1559 714.6 0 1559 208 1708 714.6 0 1708 209 1907 714.6 0 1907 210 1673 714.6 0 1673 211 1554 714.6 0 1554 212 1486 714.6 0 1486 213 1440 714.6 0 1440 214 1418 714.6 0 1418 215 1361 714.6 0 1361 216 1759 714.6 0 1759 217 2147 714.6 0 2147 218 1731 714.6 0 1731 219 1580 714.6 0 1580 220 1465 714.6 0 1465 221 1595 714.6 0 1595 222 1212 714.6 0 1212 223 1168 714.6 0 1168 224 1188 714.6 0 1188 225 1081 714.6 0 1081 226 1159 714.6 0 1159 227 1263 714.6 0 1263 228 1130 714.6 0 1130 229 1387 714.6 0 1387 230 1486 714.6 0 1486 231 1422 714.6 0 1422 232 1367 714.6 0 1367 233 1406 714.6 0 1406 234 1311 714.6 0 1311 235 1256 714.6 0 1256 236 1331 714.6 0 1331 237 1947 714.6 0 1947 238 1767 714.6 0 1767 239 1736 714.6 0 1736 240 2084 714.6 0 2084 241 1470 714.6 0 1470 242 1263 714.6 0 1263 243 1034 714.6 0 1034 244 1223 714.6 0 1223 245 1135 714.6 0 1135 246 1170 714.6 0 1170 247 1477 714.6 0 1477 248 2139 714.6 0 2139 249 1604 714.6 0 1604 250 1820 714.6 0 1820 251 1329 714.6 0 1329 252 984 714.6 0 984 253 898 714.6 0 898 254 729 714.6 0 729 255 786 714.6 0 786 256 863 714.6 0 863 257 1026 714.6 0 1026 258 1051 714.6 0 1051 259 1325 714.6 0 1325 260 1593 714.6 0 1593 261 1629 714.6 0 1629 262 1400 714.6 0 1400 263 1362 714.6 0 1362 264 1028 714.6 0 1028 265 892 714.6 0 892 266 1055 714.6 0 1055 267 1000 714.6 0 1000 268 1170 714.6 0 1170 269 1241 714.6 0 1241 270 1560 714.6 0 1560 271 1475 714.6 0 1475 272 1184 714.6 0 1184 273 1647 714.6 0 1647 274 1296 714.6 0 1296 275 1398 714.6 0 1398 276 1461 714.6 0 1461 277 1166 714.6 0 1166 278 1117 714.6 0 1117 279 1210 714.6 0 1210 280 1083 714.6 0 1083 281 1066 714.6 0 1066 282 1276 714.6 0 1276 283 964 714.6 0 964 284 1062 714.6 0 1062 285 1208 714.6 0 1208 286 964 714.6 0 964 287 1443 714.6 0 1443 288 1845 714.6 0 1845 289 1559 714.6 0 1559 290 1826 714.6 0 1826 291 1876 714.6 0 1876 292 1535 714.6 0 1535 293 1714 714.6 0 1714 294 1916 714.6 0 1916 295 1918 714.6 0 1918 296 2077 714.6 0 2077 297 2475 714.6 0 2475 298 2624 714.6 0 2624 299 2280 714.6 0 2280 300 1612 714.6 0 1612 301 2755 714.6 0 2755 Finished in 19.81 s (31 us/read; 1.94 M reads/minute). === Summary === Total reads processed: 639,259 Reads with adapters: 16,987 (2.7%) Reads that were too short: 942 (0.1%) Reads written (passing filters): 16,045 (2.5%) Total basepairs processed: 112,712,525 bp Total written (filtered): 4,034,848 bp (3.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16987 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 3 0.0 3 0 0 3 16 81 0.0 3 0 68 6 7 17 13225 0.0 3 12796 376 37 16 18 63 0.0 3 2 34 2 25 19 16 0.0 3 1 2 7 6 20 13 0.0 3 0 0 0 13 21 2 0.0 3 0 0 2 22 1 0.0 3 0 0 0 1 23 15 0.0 3 0 0 0 15 24 1 0.0 3 0 0 0 1 25 3 0.0 3 0 0 0 3 26 1550 0.0 3 0 0 8 1542 27 3 0.0 3 0 0 0 3 29 187 0.0 3 1 0 11 175 30 3 0.0 3 0 0 0 3 31 1 0.0 3 0 0 0 1 32 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 34 2 0.0 3 0 0 0 2 35 3 0.0 3 0 0 0 3 36 2 0.0 3 0 0 0 2 37 3 0.0 3 0 0 0 3 38 1 0.0 3 0 0 0 1 39 4 0.0 3 0 0 0 4 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 44 29 0.0 3 0 0 0 29 45 1 0.0 3 0 0 0 1 46 4 0.0 3 0 0 0 4 47 1 0.0 3 0 0 0 1 48 5 0.0 3 0 0 0 5 49 10 0.0 3 0 0 0 10 50 44 0.0 3 0 0 0 44 51 2 0.0 3 0 0 0 2 52 15 0.0 3 0 0 0 15 53 27 0.0 3 0 0 2 25 54 3 0.0 3 0 0 0 3 55 2 0.0 3 0 0 0 2 56 8 0.0 3 0 0 0 8 57 2 0.0 3 0 0 0 2 58 3 0.0 3 0 0 0 3 59 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 62 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 64 5 0.0 3 0 0 0 5 65 3 0.0 3 0 0 0 3 66 3 0.0 3 0 0 1 2 67 5 0.0 3 0 0 0 5 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 4 0.0 3 0 0 0 4 72 34 0.0 3 0 0 0 34 73 55 0.0 3 0 0 1 54 74 472 0.0 3 0 0 1 471 75 5 0.0 3 0 0 2 3 76 21 0.0 3 0 0 1 20 77 35 0.0 3 0 0 1 34 78 6 0.0 3 0 0 0 6 79 6 0.0 3 0 0 0 6 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 84 2 0.0 3 0 0 0 2 85 5 0.0 3 0 0 0 5 86 16 0.0 3 0 0 0 16 87 4 0.0 3 0 0 0 4 88 2 0.0 3 0 0 1 1 89 4 0.0 3 0 0 0 4 90 1 0.0 3 0 0 0 1 91 3 0.0 3 0 0 0 3 93 1 0.0 3 0 0 1 94 2 0.0 3 0 0 0 2 95 2 0.0 3 0 0 0 2 96 2 0.0 3 0 0 0 2 97 5 0.0 3 0 0 0 5 100 1 0.0 3 0 0 0 1 101 4 0.0 3 0 0 0 4 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 106 3 0.0 3 0 0 0 3 107 5 0.0 3 0 0 0 5 108 4 0.0 3 0 0 0 4 109 3 0.0 3 0 0 0 3 110 12 0.0 3 0 0 0 12 111 7 0.0 3 0 0 0 7 112 2 0.0 3 0 0 0 2 113 4 0.0 3 0 0 1 3 114 4 0.0 3 0 0 1 3 115 2 0.0 3 0 0 0 2 116 3 0.0 3 0 0 0 3 117 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 124 3 0.0 3 0 0 0 3 125 3 0.0 3 0 0 0 3 126 4 0.0 3 0 0 0 4 127 9 0.0 3 0 0 0 9 128 52 0.0 3 0 0 0 52 129 24 0.0 3 0 0 1 23 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 133 5 0.0 3 0 0 0 5 134 1 0.0 3 0 0 0 1 135 4 0.0 3 0 0 0 4 136 3 0.0 3 0 0 0 3 137 3 0.0 3 0 0 0 3 138 368 0.0 3 0 0 3 365 139 27 0.0 3 0 0 0 27 140 47 0.0 3 0 0 0 47 141 130 0.0 3 0 0 6 124 142 2 0.0 3 0 0 0 2 144 3 0.0 3 0 0 0 3 146 13 0.0 3 0 0 0 13 147 31 0.0 3 0 0 0 31 148 16 0.0 3 0 0 0 16 149 2 0.0 3 0 0 1 1 150 2 0.0 3 0 0 0 2 151 8 0.0 3 0 0 0 8 152 3 0.0 3 0 0 0 3 154 3 0.0 3 0 0 0 3 155 2 0.0 3 0 0 0 2 156 6 0.0 3 0 0 0 6 157 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 162 6 0.0 3 0 0 0 6 163 1 0.0 3 0 0 0 1 164 6 0.0 3 0 0 0 6 165 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 168 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 171 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 2 0.0 3 0 1 0 1 175 3 0.0 3 0 0 0 3 176 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 199 2 0.0 3 0 0 0 2 200 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 212 2 0.0 3 0 0 0 2 214 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 6 0.0 3 0 0 0 6 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.55 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 16,045 Reads with adapters: 1,321 (8.2%) Reads written (passing filters): 16,045 (100.0%) Total basepairs processed: 4,034,848 bp Total written (filtered): 4,030,884 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1321 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 26.2% C: 56.2% G: 13.0% T: 4.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1320 250.7 0 1320 4 1 62.7 0 1