This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.91 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 102,259 (3.5%) Reads that were too short: 45,771 (1.6%) Reads written (passing filters): 56,488 (1.9%) Total basepairs processed: 881,011,047 bp Total written (filtered): 14,916,412 bp (1.7%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 102259 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 758 714.6 0 758 7 803 714.6 0 803 8 28793 714.6 0 28793 9 12829 714.6 0 12829 10 42 714.6 0 42 11 37 714.6 0 37 12 47 714.6 0 47 13 68 714.6 0 68 14 35 714.6 0 35 15 23 714.6 0 23 16 29 714.6 0 29 17 26 714.6 0 26 18 26 714.6 0 26 19 38 714.6 0 38 20 42 714.6 0 42 21 33 714.6 0 33 22 35 714.6 0 35 23 24 714.6 0 24 24 99 714.6 0 99 25 43 714.6 0 43 26 25 714.6 0 25 27 30 714.6 0 30 28 26 714.6 0 26 29 27 714.6 0 27 30 34 714.6 0 34 31 38 714.6 0 38 32 44 714.6 0 44 33 25 714.6 0 25 34 39 714.6 0 39 35 27 714.6 0 27 36 41 714.6 0 41 37 34 714.6 0 34 38 37 714.6 0 37 39 33 714.6 0 33 40 25 714.6 0 25 41 25 714.6 0 25 42 36 714.6 0 36 43 33 714.6 0 33 44 38 714.6 0 38 45 25 714.6 0 25 46 39 714.6 0 39 47 45 714.6 0 45 48 23 714.6 0 23 49 24 714.6 0 24 50 38 714.6 0 38 51 22 714.6 0 22 52 32 714.6 0 32 53 26 714.6 0 26 54 33 714.6 0 33 55 21 714.6 0 21 56 26 714.6 0 26 57 23 714.6 0 23 58 27 714.6 0 27 59 23 714.6 0 23 60 29 714.6 0 29 61 35 714.6 0 35 62 38 714.6 0 38 63 27 714.6 0 27 64 25 714.6 0 25 65 38 714.6 0 38 66 46 714.6 0 46 67 33 714.6 0 33 68 32 714.6 0 32 69 27 714.6 0 27 70 36 714.6 0 36 71 33 714.6 0 33 72 32 714.6 0 32 73 34 714.6 0 34 74 29 714.6 0 29 75 46 714.6 0 46 76 30 714.6 0 30 77 22 714.6 0 22 78 34 714.6 0 34 79 30 714.6 0 30 80 33 714.6 0 33 81 33 714.6 0 33 82 57 714.6 0 57 83 32 714.6 0 32 84 31 714.6 0 31 85 33 714.6 0 33 86 50 714.6 0 50 87 27 714.6 0 27 88 33 714.6 0 33 89 23 714.6 0 23 90 24 714.6 0 24 91 35 714.6 0 35 92 33 714.6 0 33 93 36 714.6 0 36 94 43 714.6 0 43 95 56 714.6 0 56 96 87 714.6 0 87 97 86 714.6 0 86 98 73 714.6 0 73 99 69 714.6 0 69 100 77 714.6 0 77 101 125 714.6 0 125 102 192 714.6 0 192 103 87 714.6 0 87 104 52 714.6 0 52 105 42 714.6 0 42 106 34 714.6 0 34 107 33 714.6 0 33 108 24 714.6 0 24 109 31 714.6 0 31 110 46 714.6 0 46 111 38 714.6 0 38 112 33 714.6 0 33 113 41 714.6 0 41 114 43 714.6 0 43 115 43 714.6 0 43 116 116 714.6 0 116 117 187 714.6 0 187 118 260 714.6 0 260 119 88 714.6 0 88 120 59 714.6 0 59 121 51 714.6 0 51 122 67 714.6 0 67 123 67 714.6 0 67 124 60 714.6 0 60 125 69 714.6 0 69 126 76 714.6 0 76 127 60 714.6 0 60 128 59 714.6 0 59 129 60 714.6 0 60 130 82 714.6 0 82 131 82 714.6 0 82 132 104 714.6 0 104 133 145 714.6 0 145 134 89 714.6 0 89 135 85 714.6 0 85 136 85 714.6 0 85 137 145 714.6 0 145 138 388 714.6 0 388 139 341 714.6 0 341 140 156 714.6 0 156 141 67 714.6 0 67 142 92 714.6 0 92 143 133 714.6 0 133 144 110 714.6 0 110 145 93 714.6 0 93 146 96 714.6 0 96 147 90 714.6 0 90 148 64 714.6 0 64 149 64 714.6 0 64 150 66 714.6 0 66 151 107 714.6 0 107 152 84 714.6 0 84 153 66 714.6 0 66 154 45 714.6 0 45 155 82 714.6 0 82 156 95 714.6 0 95 157 126 714.6 0 126 158 129 714.6 0 129 159 120 714.6 0 120 160 108 714.6 0 108 161 88 714.6 0 88 162 73 714.6 0 73 163 57 714.6 0 57 164 70 714.6 0 70 165 87 714.6 0 87 166 75 714.6 0 75 167 101 714.6 0 101 168 91 714.6 0 91 169 78 714.6 0 78 170 117 714.6 0 117 171 126 714.6 0 126 172 90 714.6 0 90 173 81 714.6 0 81 174 75 714.6 0 75 175 89 714.6 0 89 176 84 714.6 0 84 177 110 714.6 0 110 178 83 714.6 0 83 179 154 714.6 0 154 180 151 714.6 0 151 181 119 714.6 0 119 182 83 714.6 0 83 183 103 714.6 0 103 184 162 714.6 0 162 185 104 714.6 0 104 186 105 714.6 0 105 187 208 714.6 0 208 188 165 714.6 0 165 189 85 714.6 0 85 190 52 714.6 0 52 191 64 714.6 0 64 192 107 714.6 0 107 193 100 714.6 0 100 194 140 714.6 0 140 195 140 714.6 0 140 196 100 714.6 0 100 197 89 714.6 0 89 198 105 714.6 0 105 199 176 714.6 0 176 200 149 714.6 0 149 201 69 714.6 0 69 202 48 714.6 0 48 203 59 714.6 0 59 204 92 714.6 0 92 205 117 714.6 0 117 206 141 714.6 0 141 207 104 714.6 0 104 208 118 714.6 0 118 209 80 714.6 0 80 210 103 714.6 0 103 211 87 714.6 0 87 212 130 714.6 0 130 213 129 714.6 0 129 214 177 714.6 0 177 215 193 714.6 0 193 216 153 714.6 0 153 217 112 714.6 0 112 218 124 714.6 0 124 219 141 714.6 0 141 220 138 714.6 0 138 221 105 714.6 0 105 222 104 714.6 0 104 223 83 714.6 0 83 224 90 714.6 0 90 225 148 714.6 0 148 226 91 714.6 0 91 227 145 714.6 0 145 228 157 714.6 0 157 229 135 714.6 0 135 230 104 714.6 0 104 231 75 714.6 0 75 232 78 714.6 0 78 233 91 714.6 0 91 234 70 714.6 0 70 235 66 714.6 0 66 236 104 714.6 0 104 237 143 714.6 0 143 238 138 714.6 0 138 239 142 714.6 0 142 240 158 714.6 0 158 241 116 714.6 0 116 242 72 714.6 0 72 243 82 714.6 0 82 244 83 714.6 0 83 245 85 714.6 0 85 246 71 714.6 0 71 247 85 714.6 0 85 248 166 714.6 0 166 249 140 714.6 0 140 250 73 714.6 0 73 251 38 714.6 0 38 252 33 714.6 0 33 253 49 714.6 0 49 254 75 714.6 0 75 255 95 714.6 0 95 256 99 714.6 0 99 257 124 714.6 0 124 258 122 714.6 0 122 259 112 714.6 0 112 260 178 714.6 0 178 261 94 714.6 0 94 262 132 714.6 0 132 263 168 714.6 0 168 264 141 714.6 0 141 265 119 714.6 0 119 266 145 714.6 0 145 267 134 714.6 0 134 268 185 714.6 0 185 269 166 714.6 0 166 270 113 714.6 0 113 271 109 714.6 0 109 272 141 714.6 0 141 273 1702 714.6 0 1702 274 1439 714.6 0 1439 275 2727 714.6 0 2727 276 219 714.6 0 219 277 166 714.6 0 166 278 135 714.6 0 135 279 178 714.6 0 178 280 480 714.6 0 480 281 212 714.6 0 212 282 189 714.6 0 189 283 365 714.6 0 365 284 290 714.6 0 290 285 338 714.6 0 338 286 319 714.6 0 319 287 655 714.6 0 655 288 396 714.6 0 396 289 252 714.6 0 252 290 209 714.6 0 209 291 202 714.6 0 202 292 316 714.6 0 316 293 5174 714.6 0 5174 294 2595 714.6 0 2595 295 10629 714.6 0 10629 296 403 714.6 0 403 297 653 714.6 0 653 298 1957 714.6 0 1957 299 2380 714.6 0 2380 300 2567 714.6 0 2567 301 606 714.6 0 606 Finished in 2.89 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 56,488 Reads with adapters: 14,056 (24.9%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 14,051 (24.9%) Total basepairs processed: 14,916,412 bp Total written (filtered): 3,807,415 bp (25.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14056 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 2 0.0 3 0 0 0 0 2 18 8 0.0 3 0 0 7 1 19 159 0.0 3 0 158 1 20 13278 0.0 4 13115 157 5 0 1 21 32 0.0 4 5 24 1 1 1 22 418 0.0 4 409 7 2 23 2 0.0 4 0 0 0 1 1 46 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 107 3 0.0 4 0 0 0 2 1 109 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 1 1 112 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 138 0.0 4 0 0 0 0 138 142 1 0.0 4 0 1 157 1 0.0 4 0 0 0 0 1 247 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.47 s (33 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 14,051 Reads with adapters: 416 (3.0%) Reads written (passing filters): 14,051 (100.0%) Total basepairs processed: 3,807,415 bp Total written (filtered): 3,799,502 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 416 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 5.0% G: 92.1% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 219.5 0 2 4 1 54.9 0 1 6 1 3.4 0 1 9 7 0.1 0 1 6 10 265 0.0 1 9 256 11 122 0.0 1 0 122 13 1 0.0 1 0 1 223 15 0.0 1 15 224 1 0.0 1 1 260 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.