This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.87 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 62,115 (2.1%) Reads that were too short: 10,420 (0.4%) Reads written (passing filters): 51,695 (1.8%) Total basepairs processed: 881,011,047 bp Total written (filtered): 8,365,085 bp (0.9%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 62115 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 98 714.6 0 98 7 1695 714.6 0 1695 8 143 714.6 0 143 9 4449 714.6 0 4449 10 37 714.6 0 37 11 30 714.6 0 30 12 44 714.6 0 44 13 25 714.6 0 25 14 41 714.6 0 41 15 29 714.6 0 29 16 33 714.6 0 33 17 34 714.6 0 34 18 28 714.6 0 28 19 86 714.6 0 86 20 23 714.6 0 23 21 30 714.6 0 30 22 42 714.6 0 42 23 34 714.6 0 34 24 25 714.6 0 25 25 17 714.6 0 17 26 25 714.6 0 25 27 21 714.6 0 21 28 39 714.6 0 39 29 39 714.6 0 39 30 22 714.6 0 22 31 28 714.6 0 28 32 49 714.6 0 49 33 138 714.6 0 138 34 148 714.6 0 148 35 105 714.6 0 105 36 19 714.6 0 19 37 23 714.6 0 23 38 23 714.6 0 23 39 18 714.6 0 18 40 23 714.6 0 23 41 22 714.6 0 22 42 16 714.6 0 16 43 34 714.6 0 34 44 31 714.6 0 31 45 70 714.6 0 70 46 35 714.6 0 35 47 33 714.6 0 33 48 14 714.6 0 14 49 36 714.6 0 36 50 31 714.6 0 31 51 34 714.6 0 34 52 22 714.6 0 22 53 33 714.6 0 33 54 26 714.6 0 26 55 23 714.6 0 23 56 33 714.6 0 33 57 87 714.6 0 87 58 35 714.6 0 35 59 39 714.6 0 39 60 49 714.6 0 49 61 21 714.6 0 21 62 19 714.6 0 19 63 32 714.6 0 32 64 20 714.6 0 20 65 28 714.6 0 28 66 20 714.6 0 20 67 31 714.6 0 31 68 31 714.6 0 31 69 18 714.6 0 18 70 24 714.6 0 24 71 22 714.6 0 22 72 125 714.6 0 125 73 37 714.6 0 37 74 38 714.6 0 38 75 32 714.6 0 32 76 22 714.6 0 22 77 33 714.6 0 33 78 35 714.6 0 35 79 32 714.6 0 32 80 23 714.6 0 23 81 26 714.6 0 26 82 32 714.6 0 32 83 28 714.6 0 28 84 146 714.6 0 146 85 100 714.6 0 100 86 68 714.6 0 68 87 68 714.6 0 68 88 113 714.6 0 113 89 46 714.6 0 46 90 54 714.6 0 54 91 65 714.6 0 65 92 59 714.6 0 59 93 81 714.6 0 81 94 101 714.6 0 101 95 62 714.6 0 62 96 73 714.6 0 73 97 119 714.6 0 119 98 89 714.6 0 89 99 67 714.6 0 67 100 62 714.6 0 62 101 31 714.6 0 31 102 50 714.6 0 50 103 63 714.6 0 63 104 43 714.6 0 43 105 75 714.6 0 75 106 458 714.6 0 458 107 1502 714.6 0 1502 108 1701 714.6 0 1701 109 1200 714.6 0 1200 110 41 714.6 0 41 111 55 714.6 0 55 112 37 714.6 0 37 113 22 714.6 0 22 114 46 714.6 0 46 115 63 714.6 0 63 116 56 714.6 0 56 117 52 714.6 0 52 118 35 714.6 0 35 119 43 714.6 0 43 120 45 714.6 0 45 121 149 714.6 0 149 122 96 714.6 0 96 123 59 714.6 0 59 124 46 714.6 0 46 125 44 714.6 0 44 126 55 714.6 0 55 127 149 714.6 0 149 128 455 714.6 0 455 129 500 714.6 0 500 130 121 714.6 0 121 131 103 714.6 0 103 132 51 714.6 0 51 133 49 714.6 0 49 134 89 714.6 0 89 135 115 714.6 0 115 136 312 714.6 0 312 137 276 714.6 0 276 138 137 714.6 0 137 139 74 714.6 0 74 140 98 714.6 0 98 141 177 714.6 0 177 142 161 714.6 0 161 143 302 714.6 0 302 144 726 714.6 0 726 145 393 714.6 0 393 146 104 714.6 0 104 147 157 714.6 0 157 148 62 714.6 0 62 149 80 714.6 0 80 150 75 714.6 0 75 151 61 714.6 0 61 152 103 714.6 0 103 153 375 714.6 0 375 154 351 714.6 0 351 155 290 714.6 0 290 156 201 714.6 0 201 157 112 714.6 0 112 158 85 714.6 0 85 159 97 714.6 0 97 160 83 714.6 0 83 161 102 714.6 0 102 162 102 714.6 0 102 163 65 714.6 0 65 164 50 714.6 0 50 165 90 714.6 0 90 166 116 714.6 0 116 167 116 714.6 0 116 168 101 714.6 0 101 169 188 714.6 0 188 170 186 714.6 0 186 171 87 714.6 0 87 172 57 714.6 0 57 173 73 714.6 0 73 174 151 714.6 0 151 175 190 714.6 0 190 176 147 714.6 0 147 177 86 714.6 0 86 178 78 714.6 0 78 179 145 714.6 0 145 180 112 714.6 0 112 181 212 714.6 0 212 182 238 714.6 0 238 183 348 714.6 0 348 184 381 714.6 0 381 185 843 714.6 0 843 186 19635 714.6 0 19635 187 2520 714.6 0 2520 188 1460 714.6 0 1460 189 64 714.6 0 64 190 44 714.6 0 44 191 87 714.6 0 87 192 71 714.6 0 71 193 62 714.6 0 62 194 67 714.6 0 67 195 54 714.6 0 54 196 38 714.6 0 38 197 46 714.6 0 46 198 42 714.6 0 42 199 65 714.6 0 65 200 66 714.6 0 66 201 51 714.6 0 51 202 67 714.6 0 67 203 100 714.6 0 100 204 256 714.6 0 256 205 251 714.6 0 251 206 105 714.6 0 105 207 96 714.6 0 96 208 130 714.6 0 130 209 106 714.6 0 106 210 85 714.6 0 85 211 104 714.6 0 104 212 72 714.6 0 72 213 87 714.6 0 87 214 92 714.6 0 92 215 97 714.6 0 97 216 117 714.6 0 117 217 642 714.6 0 642 218 1311 714.6 0 1311 219 406 714.6 0 406 220 58 714.6 0 58 221 84 714.6 0 84 222 85 714.6 0 85 223 69 714.6 0 69 224 59 714.6 0 59 225 63 714.6 0 63 226 87 714.6 0 87 227 173 714.6 0 173 228 89 714.6 0 89 229 68 714.6 0 68 230 49 714.6 0 49 231 60 714.6 0 60 232 84 714.6 0 84 233 62 714.6 0 62 234 108 714.6 0 108 235 74 714.6 0 74 236 68 714.6 0 68 237 70 714.6 0 70 238 61 714.6 0 61 239 46 714.6 0 46 240 31 714.6 0 31 241 35 714.6 0 35 242 63 714.6 0 63 243 89 714.6 0 89 244 134 714.6 0 134 245 284 714.6 0 284 246 89 714.6 0 89 247 47 714.6 0 47 248 44 714.6 0 44 249 21 714.6 0 21 250 42 714.6 0 42 251 94 714.6 0 94 252 137 714.6 0 137 253 216 714.6 0 216 254 170 714.6 0 170 255 124 714.6 0 124 256 84 714.6 0 84 257 82 714.6 0 82 258 62 714.6 0 62 259 36 714.6 0 36 260 38 714.6 0 38 261 36 714.6 0 36 262 95 714.6 0 95 263 100 714.6 0 100 264 83 714.6 0 83 265 72 714.6 0 72 266 87 714.6 0 87 267 81 714.6 0 81 268 65 714.6 0 65 269 39 714.6 0 39 270 47 714.6 0 47 271 53 714.6 0 53 272 100 714.6 0 100 273 59 714.6 0 59 274 32 714.6 0 32 275 40 714.6 0 40 276 33 714.6 0 33 277 82 714.6 0 82 278 141 714.6 0 141 279 115 714.6 0 115 280 212 714.6 0 212 281 111 714.6 0 111 282 98 714.6 0 98 283 36 714.6 0 36 284 63 714.6 0 63 285 138 714.6 0 138 286 64 714.6 0 64 287 39 714.6 0 39 288 29 714.6 0 29 289 32 714.6 0 32 290 30 714.6 0 30 291 57 714.6 0 57 292 49 714.6 0 49 293 34 714.6 0 34 294 37 714.6 0 37 295 42 714.6 0 42 296 57 714.6 0 57 297 109 714.6 0 109 298 149 714.6 0 149 299 73 714.6 0 73 300 23 714.6 0 23 301 15 714.6 0 15 Finished in 1.68 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 51,695 Reads with adapters: 4,624 (8.9%) Reads that were too short: 32 (0.1%) Reads written (passing filters): 4,592 (8.9%) Total basepairs processed: 8,365,085 bp Total written (filtered): 1,256,022 bp (15.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4624 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 4 0.0 3 0 0 3 1 16 42 0.0 3 0 38 1 3 17 4475 0.0 3 4334 123 16 2 18 10 0.0 3 0 6 4 19 7 0.0 3 0 0 4 3 20 2 0.0 3 0 0 0 2 31 6 0.0 3 0 0 0 6 44 1 0.0 3 0 0 0 1 48 9 0.0 3 0 0 0 9 51 4 0.0 3 0 0 0 4 55 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 1 59 9 0.0 3 0 0 0 9 61 3 0.0 3 0 0 0 3 64 2 0.0 3 0 0 0 2 67 4 0.0 3 0 0 0 4 70 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 109 7 0.0 3 0 0 0 7 110 2 0.0 3 0 0 1 1 111 3 0.0 3 0 0 0 3 115 1 0.0 3 0 0 0 1 119 3 0.0 3 0 0 0 3 132 3 0.0 3 0 0 0 3 134 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 150 5 0.0 3 0 0 0 5 161 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 4,592 Reads with adapters: 151 (3.3%) Reads written (passing filters): 4,592 (100.0%) Total basepairs processed: 1,256,022 bp Total written (filtered): 1,255,565 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 151 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.3% C: 7.9% G: 27.2% T: 12.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 147 71.8 0 147 4 4 17.9 0 4