This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.57 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 51,284 (1.8%) Reads that were too short: 17,306 (0.6%) Reads written (passing filters): 33,978 (1.2%) Total basepairs processed: 881,011,047 bp Total written (filtered): 7,163,561 bp (0.8%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 51284 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 191 714.6 0 191 7 5003 714.6 0 5003 8 4934 714.6 0 4934 9 35 714.6 0 35 10 35 714.6 0 35 11 43 714.6 0 43 12 33 714.6 0 33 13 41 714.6 0 41 14 38 714.6 0 38 15 29 714.6 0 29 16 29 714.6 0 29 17 38 714.6 0 38 18 35 714.6 0 35 19 45 714.6 0 45 20 44 714.6 0 44 21 37 714.6 0 37 22 32 714.6 0 32 23 35 714.6 0 35 24 39 714.6 0 39 25 31 714.6 0 31 26 30 714.6 0 30 27 48 714.6 0 48 28 38 714.6 0 38 29 46 714.6 0 46 30 81 714.6 0 81 31 121 714.6 0 121 32 211 714.6 0 211 33 57 714.6 0 57 34 49 714.6 0 49 35 59 714.6 0 59 36 46 714.6 0 46 37 65 714.6 0 65 38 54 714.6 0 54 39 81 714.6 0 81 40 72 714.6 0 72 41 63 714.6 0 63 42 81 714.6 0 81 43 61 714.6 0 61 44 57 714.6 0 57 45 80 714.6 0 80 46 63 714.6 0 63 47 71 714.6 0 71 48 97 714.6 0 97 49 64 714.6 0 64 50 318 714.6 0 318 51 643 714.6 0 643 52 523 714.6 0 523 53 64 714.6 0 64 54 72 714.6 0 72 55 65 714.6 0 65 56 50 714.6 0 50 57 50 714.6 0 50 58 68 714.6 0 68 59 328 714.6 0 328 60 71 714.6 0 71 61 69 714.6 0 69 62 45 714.6 0 45 63 170 714.6 0 170 64 58 714.6 0 58 65 774 714.6 0 774 66 83 714.6 0 83 67 73 714.6 0 73 68 54 714.6 0 54 69 58 714.6 0 58 70 70 714.6 0 70 71 62 714.6 0 62 72 63 714.6 0 63 73 72 714.6 0 72 74 87 714.6 0 87 75 265 714.6 0 265 76 178 714.6 0 178 77 49 714.6 0 49 78 66 714.6 0 66 79 63 714.6 0 63 80 60 714.6 0 60 81 53 714.6 0 53 82 59 714.6 0 59 83 79 714.6 0 79 84 62 714.6 0 62 85 68 714.6 0 68 86 48 714.6 0 48 87 62 714.6 0 62 88 56 714.6 0 56 89 41 714.6 0 41 90 21 714.6 0 21 91 42 714.6 0 42 92 32 714.6 0 32 93 55 714.6 0 55 94 62 714.6 0 62 95 68 714.6 0 68 96 70 714.6 0 70 97 78 714.6 0 78 98 90 714.6 0 90 99 66 714.6 0 66 100 72 714.6 0 72 101 64 714.6 0 64 102 75 714.6 0 75 103 75 714.6 0 75 104 65 714.6 0 65 105 65 714.6 0 65 106 43 714.6 0 43 107 38 714.6 0 38 108 32 714.6 0 32 109 65 714.6 0 65 110 48 714.6 0 48 111 52 714.6 0 52 112 73 714.6 0 73 113 78 714.6 0 78 114 276 714.6 0 276 115 166 714.6 0 166 116 173 714.6 0 173 117 72 714.6 0 72 118 109 714.6 0 109 119 95 714.6 0 95 120 84 714.6 0 84 121 83 714.6 0 83 122 97 714.6 0 97 123 85 714.6 0 85 124 83 714.6 0 83 125 120 714.6 0 120 126 294 714.6 0 294 127 220 714.6 0 220 128 89 714.6 0 89 129 124 714.6 0 124 130 148 714.6 0 148 131 118 714.6 0 118 132 193 714.6 0 193 133 137 714.6 0 137 134 135 714.6 0 135 135 529 714.6 0 529 136 365 714.6 0 365 137 579 714.6 0 579 138 114 714.6 0 114 139 88 714.6 0 88 140 107 714.6 0 107 141 105 714.6 0 105 142 119 714.6 0 119 143 90 714.6 0 90 144 151 714.6 0 151 145 123 714.6 0 123 146 163 714.6 0 163 147 111 714.6 0 111 148 91 714.6 0 91 149 84 714.6 0 84 150 79 714.6 0 79 151 57 714.6 0 57 152 60 714.6 0 60 153 70 714.6 0 70 154 78 714.6 0 78 155 93 714.6 0 93 156 87 714.6 0 87 157 109 714.6 0 109 158 83 714.6 0 83 159 104 714.6 0 104 160 126 714.6 0 126 161 83 714.6 0 83 162 109 714.6 0 109 163 87 714.6 0 87 164 143 714.6 0 143 165 220 714.6 0 220 166 184 714.6 0 184 167 117 714.6 0 117 168 227 714.6 0 227 169 261 714.6 0 261 170 124 714.6 0 124 171 134 714.6 0 134 172 117 714.6 0 117 173 105 714.6 0 105 174 109 714.6 0 109 175 137 714.6 0 137 176 107 714.6 0 107 177 104 714.6 0 104 178 86 714.6 0 86 179 90 714.6 0 90 180 128 714.6 0 128 181 176 714.6 0 176 182 149 714.6 0 149 183 177 714.6 0 177 184 127 714.6 0 127 185 169 714.6 0 169 186 144 714.6 0 144 187 145 714.6 0 145 188 104 714.6 0 104 189 96 714.6 0 96 190 94 714.6 0 94 191 144 714.6 0 144 192 186 714.6 0 186 193 245 714.6 0 245 194 128 714.6 0 128 195 210 714.6 0 210 196 218 714.6 0 218 197 544 714.6 0 544 198 1450 714.6 0 1450 199 358 714.6 0 358 200 544 714.6 0 544 201 162 714.6 0 162 202 101 714.6 0 101 203 114 714.6 0 114 204 388 714.6 0 388 205 209 714.6 0 209 206 152 714.6 0 152 207 97 714.6 0 97 208 104 714.6 0 104 209 115 714.6 0 115 210 84 714.6 0 84 211 81 714.6 0 81 212 115 714.6 0 115 213 141 714.6 0 141 214 118 714.6 0 118 215 151 714.6 0 151 216 321 714.6 0 321 217 292 714.6 0 292 218 444 714.6 0 444 219 212 714.6 0 212 220 213 714.6 0 213 221 197 714.6 0 197 222 130 714.6 0 130 223 123 714.6 0 123 224 122 714.6 0 122 225 167 714.6 0 167 226 135 714.6 0 135 227 167 714.6 0 167 228 155 714.6 0 155 229 152 714.6 0 152 230 122 714.6 0 122 231 97 714.6 0 97 232 104 714.6 0 104 233 105 714.6 0 105 234 100 714.6 0 100 235 120 714.6 0 120 236 121 714.6 0 121 237 199 714.6 0 199 238 176 714.6 0 176 239 259 714.6 0 259 240 201 714.6 0 201 241 157 714.6 0 157 242 79 714.6 0 79 243 83 714.6 0 83 244 93 714.6 0 93 245 111 714.6 0 111 246 200 714.6 0 200 247 863 714.6 0 863 248 1990 714.6 0 1990 249 647 714.6 0 647 250 233 714.6 0 233 251 72 714.6 0 72 252 57 714.6 0 57 253 72 714.6 0 72 254 147 714.6 0 147 255 150 714.6 0 150 256 134 714.6 0 134 257 165 714.6 0 165 258 176 714.6 0 176 259 149 714.6 0 149 260 103 714.6 0 103 261 82 714.6 0 82 262 87 714.6 0 87 263 74 714.6 0 74 264 101 714.6 0 101 265 128 714.6 0 128 266 156 714.6 0 156 267 179 714.6 0 179 268 190 714.6 0 190 269 98 714.6 0 98 270 90 714.6 0 90 271 88 714.6 0 88 272 82 714.6 0 82 273 100 714.6 0 100 274 131 714.6 0 131 275 126 714.6 0 126 276 126 714.6 0 126 277 97 714.6 0 97 278 115 714.6 0 115 279 104 714.6 0 104 280 96 714.6 0 96 281 169 714.6 0 169 282 82 714.6 0 82 283 85 714.6 0 85 284 85 714.6 0 85 285 144 714.6 0 144 286 168 714.6 0 168 287 155 714.6 0 155 288 161 714.6 0 161 289 157 714.6 0 157 290 156 714.6 0 156 291 174 714.6 0 174 292 150 714.6 0 150 293 185 714.6 0 185 294 243 714.6 0 243 295 187 714.6 0 187 296 99 714.6 0 99 297 95 714.6 0 95 298 92 714.6 0 92 299 83 714.6 0 83 300 105 714.6 0 105 301 196 714.6 0 196 Finished in 1.45 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 33,978 Reads with adapters: 5,017 (14.8%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 5,004 (14.7%) Total basepairs processed: 7,163,561 bp Total written (filtered): 1,363,621 bp (19.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5017 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 32 0.0 3 0 0 0 1 31 18 2 0.0 3 0 0 2 19 46 0.0 3 0 45 0 0 1 20 4880 0.0 4 4807 63 1 0 9 21 21 0.0 4 2 12 0 0 7 22 3 0.0 4 0 0 1 0 2 23 3 0.0 4 0 0 0 2 1 24 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 1 73 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 92 16 0.0 4 0 0 0 0 16 97 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 5,004 Reads with adapters: 280 (5.6%) Reads written (passing filters): 5,004 (100.0%) Total basepairs processed: 1,363,621 bp Total written (filtered): 1,351,178 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 280 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 15.4% G: 82.1% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 78.2 0 7 10 13 0.0 1 0 13 11 223 0.0 1 0 223 151 1 0.0 1 0 1 268 1 0.0 1 1 269 31 0.0 1 31 270 3 0.0 1 2 1 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.