This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.59 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 68,305 (2.3%) Reads that were too short: 9,245 (0.3%) Reads written (passing filters): 59,060 (2.0%) Total basepairs processed: 881,011,047 bp Total written (filtered): 14,843,527 bp (1.7%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 68305 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 377 714.6 0 377 7 738 714.6 0 738 8 23014 714.6 0 23014 9 8653 714.6 0 8653 10 131 714.6 0 131 11 53 714.6 0 53 12 57 714.6 0 57 13 58 714.6 0 58 14 62 714.6 0 62 15 73 714.6 0 73 16 85 714.6 0 85 17 54 714.6 0 54 18 77 714.6 0 77 19 58 714.6 0 58 20 56 714.6 0 56 21 54 714.6 0 54 22 67 714.6 0 67 23 58 714.6 0 58 24 50 714.6 0 50 25 54 714.6 0 54 26 53 714.6 0 53 27 47 714.6 0 47 28 47 714.6 0 47 29 56 714.6 0 56 30 53 714.6 0 53 31 62 714.6 0 62 32 48 714.6 0 48 33 55 714.6 0 55 34 45 714.6 0 45 35 53 714.6 0 53 36 53 714.6 0 53 37 56 714.6 0 56 38 51 714.6 0 51 39 63 714.6 0 63 40 91 714.6 0 91 41 45 714.6 0 45 42 90 714.6 0 90 43 49 714.6 0 49 44 45 714.6 0 45 45 55 714.6 0 55 46 70 714.6 0 70 47 48 714.6 0 48 48 84 714.6 0 84 49 4986 714.6 0 4986 50 727 714.6 0 727 51 42 714.6 0 42 52 54 714.6 0 54 53 49 714.6 0 49 54 49 714.6 0 49 55 38 714.6 0 38 56 22 714.6 0 22 57 35 714.6 0 35 58 27 714.6 0 27 59 30 714.6 0 30 60 38 714.6 0 38 61 55 714.6 0 55 62 30 714.6 0 30 63 32 714.6 0 32 64 36 714.6 0 36 65 36 714.6 0 36 66 37 714.6 0 37 67 43 714.6 0 43 68 44 714.6 0 44 69 14 714.6 0 14 70 44 714.6 0 44 71 32 714.6 0 32 72 24 714.6 0 24 73 33 714.6 0 33 74 28 714.6 0 28 75 28 714.6 0 28 76 23 714.6 0 23 77 26 714.6 0 26 78 33 714.6 0 33 79 33 714.6 0 33 80 44 714.6 0 44 81 34 714.6 0 34 82 91 714.6 0 91 83 95 714.6 0 95 84 80 714.6 0 80 85 94 714.6 0 94 86 103 714.6 0 103 87 60 714.6 0 60 88 43 714.6 0 43 89 89 714.6 0 89 90 70 714.6 0 70 91 187 714.6 0 187 92 175 714.6 0 175 93 100 714.6 0 100 94 78 714.6 0 78 95 91 714.6 0 91 96 55 714.6 0 55 97 101 714.6 0 101 98 293 714.6 0 293 99 86 714.6 0 86 100 44 714.6 0 44 101 34 714.6 0 34 102 52 714.6 0 52 103 45 714.6 0 45 104 72 714.6 0 72 105 85 714.6 0 85 106 88 714.6 0 88 107 97 714.6 0 97 108 46 714.6 0 46 109 75 714.6 0 75 110 180 714.6 0 180 111 3509 714.6 0 3509 112 211 714.6 0 211 113 238 714.6 0 238 114 88 714.6 0 88 115 270 714.6 0 270 116 372 714.6 0 372 117 302 714.6 0 302 118 1323 714.6 0 1323 119 751 714.6 0 751 120 58 714.6 0 58 121 48 714.6 0 48 122 58 714.6 0 58 123 38 714.6 0 38 124 46 714.6 0 46 125 107 714.6 0 107 126 98 714.6 0 98 127 108 714.6 0 108 128 57 714.6 0 57 129 51 714.6 0 51 130 57 714.6 0 57 131 84 714.6 0 84 132 74 714.6 0 74 133 81 714.6 0 81 134 54 714.6 0 54 135 61 714.6 0 61 136 49 714.6 0 49 137 46 714.6 0 46 138 72 714.6 0 72 139 74 714.6 0 74 140 50 714.6 0 50 141 54 714.6 0 54 142 72 714.6 0 72 143 64 714.6 0 64 144 78 714.6 0 78 145 82 714.6 0 82 146 95 714.6 0 95 147 76 714.6 0 76 148 118 714.6 0 118 149 92 714.6 0 92 150 82 714.6 0 82 151 229 714.6 0 229 152 595 714.6 0 595 153 282 714.6 0 282 154 107 714.6 0 107 155 101 714.6 0 101 156 67 714.6 0 67 157 97 714.6 0 97 158 51 714.6 0 51 159 84 714.6 0 84 160 45 714.6 0 45 161 73 714.6 0 73 162 114 714.6 0 114 163 98 714.6 0 98 164 93 714.6 0 93 165 62 714.6 0 62 166 55 714.6 0 55 167 73 714.6 0 73 168 77 714.6 0 77 169 85 714.6 0 85 170 79 714.6 0 79 171 102 714.6 0 102 172 117 714.6 0 117 173 82 714.6 0 82 174 59 714.6 0 59 175 55 714.6 0 55 176 63 714.6 0 63 177 83 714.6 0 83 178 96 714.6 0 96 179 133 714.6 0 133 180 211 714.6 0 211 181 189 714.6 0 189 182 153 714.6 0 153 183 91 714.6 0 91 184 90 714.6 0 90 185 67 714.6 0 67 186 43 714.6 0 43 187 55 714.6 0 55 188 73 714.6 0 73 189 66 714.6 0 66 190 83 714.6 0 83 191 86 714.6 0 86 192 90 714.6 0 90 193 53 714.6 0 53 194 44 714.6 0 44 195 89 714.6 0 89 196 67 714.6 0 67 197 65 714.6 0 65 198 46 714.6 0 46 199 84 714.6 0 84 200 107 714.6 0 107 201 40 714.6 0 40 202 52 714.6 0 52 203 40 714.6 0 40 204 59 714.6 0 59 205 69 714.6 0 69 206 71 714.6 0 71 207 59 714.6 0 59 208 62 714.6 0 62 209 58 714.6 0 58 210 73 714.6 0 73 211 91 714.6 0 91 212 105 714.6 0 105 213 118 714.6 0 118 214 116 714.6 0 116 215 115 714.6 0 115 216 108 714.6 0 108 217 140 714.6 0 140 218 294 714.6 0 294 219 374 714.6 0 374 220 127 714.6 0 127 221 110 714.6 0 110 222 68 714.6 0 68 223 75 714.6 0 75 224 105 714.6 0 105 225 115 714.6 0 115 226 112 714.6 0 112 227 86 714.6 0 86 228 100 714.6 0 100 229 152 714.6 0 152 230 97 714.6 0 97 231 119 714.6 0 119 232 155 714.6 0 155 233 236 714.6 0 236 234 125 714.6 0 125 235 204 714.6 0 204 236 162 714.6 0 162 237 178 714.6 0 178 238 103 714.6 0 103 239 92 714.6 0 92 240 63 714.6 0 63 241 70 714.6 0 70 242 54 714.6 0 54 243 61 714.6 0 61 244 84 714.6 0 84 245 151 714.6 0 151 246 145 714.6 0 145 247 106 714.6 0 106 248 111 714.6 0 111 249 129 714.6 0 129 250 104 714.6 0 104 251 53 714.6 0 53 252 74 714.6 0 74 253 75 714.6 0 75 254 172 714.6 0 172 255 169 714.6 0 169 256 145 714.6 0 145 257 75 714.6 0 75 258 64 714.6 0 64 259 67 714.6 0 67 260 44 714.6 0 44 261 39 714.6 0 39 262 37 714.6 0 37 263 42 714.6 0 42 264 63 714.6 0 63 265 95 714.6 0 95 266 82 714.6 0 82 267 79 714.6 0 79 268 75 714.6 0 75 269 75 714.6 0 75 270 73 714.6 0 73 271 47 714.6 0 47 272 57 714.6 0 57 273 39 714.6 0 39 274 43 714.6 0 43 275 63 714.6 0 63 276 74 714.6 0 74 277 62 714.6 0 62 278 51 714.6 0 51 279 72 714.6 0 72 280 90 714.6 0 90 281 108 714.6 0 108 282 88 714.6 0 88 283 99 714.6 0 99 284 114 714.6 0 114 285 61 714.6 0 61 286 67 714.6 0 67 287 100 714.6 0 100 288 85 714.6 0 85 289 79 714.6 0 79 290 52 714.6 0 52 291 52 714.6 0 52 292 64 714.6 0 64 293 95 714.6 0 95 294 75 714.6 0 75 295 66 714.6 0 66 296 60 714.6 0 60 297 42 714.6 0 42 298 39 714.6 0 39 299 29 714.6 0 29 300 52 714.6 0 52 301 19 714.6 0 19 Finished in 2.60 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 59,060 Reads with adapters: 23,483 (39.8%) Reads that were too short: 58 (0.1%) Reads written (passing filters): 23,425 (39.7%) Total basepairs processed: 14,843,527 bp Total written (filtered): 6,457,678 bp (43.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 23483 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 8 0.0 3 0 0 5 3 16 245 0.0 3 0 212 22 11 17 22952 0.0 3 22012 802 112 26 18 62 0.0 3 7 28 20 7 19 58 0.0 3 2 1 28 27 20 33 0.0 3 0 0 1 32 25 1 0.0 3 1 26 2 0.0 3 0 0 0 2 27 3 0.0 3 0 0 0 3 34 1 0.0 3 1 37 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 9 0.0 3 0 0 1 8 41 1 0.0 3 0 0 0 1 42 1 0.0 3 1 45 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 54 14 0.0 3 0 0 0 14 55 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 57 4 0.0 3 0 0 0 4 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 106 3 0.0 3 0 0 0 3 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 116 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 1 138 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 157 3 0.0 3 0 0 0 3 166 6 0.0 3 0 0 0 6 167 4 0.0 3 0 0 0 4 168 1 0.0 3 0 0 0 1 169 18 0.0 3 0 0 1 17 170 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.81 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 23,425 Reads with adapters: 377 (1.6%) Reads written (passing filters): 23,425 (100.0%) Total basepairs processed: 6,457,678 bp Total written (filtered): 6,456,537 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 377 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 54.9% C: 14.6% G: 24.4% T: 6.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 368 366.0 0 368 4 8 91.5 0 8 5 1 22.9 0 1