This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.83 s (25 us/read; 2.38 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 262,702 (9.0%) Reads that were too short: 8,358 (0.3%) Reads written (passing filters): 254,344 (8.7%) Total basepairs processed: 881,011,047 bp Total written (filtered): 62,194,673 bp (7.1%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 262702 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 380 714.6 0 380 7 5679 714.6 0 5679 8 18808 714.6 0 18808 9 231 714.6 0 231 10 233 714.6 0 233 11 142 714.6 0 142 12 141 714.6 0 141 13 215 714.6 0 215 14 211 714.6 0 211 15 217 714.6 0 217 16 236 714.6 0 236 17 296 714.6 0 296 18 317 714.6 0 317 19 417 714.6 0 417 20 5859 714.6 0 5859 21 15149 714.6 0 15149 22 11100 714.6 0 11100 23 155 714.6 0 155 24 132 714.6 0 132 25 160 714.6 0 160 26 88 714.6 0 88 27 81 714.6 0 81 28 69 714.6 0 69 29 136 714.6 0 136 30 117 714.6 0 117 31 177 714.6 0 177 32 196 714.6 0 196 33 292 714.6 0 292 34 331 714.6 0 331 35 653 714.6 0 653 36 1669 714.6 0 1669 37 345 714.6 0 345 38 547 714.6 0 547 39 1350 714.6 0 1350 40 586 714.6 0 586 41 564 714.6 0 564 42 885 714.6 0 885 43 842 714.6 0 842 44 1033 714.6 0 1033 45 915 714.6 0 915 46 1144 714.6 0 1144 47 1406 714.6 0 1406 48 2551 714.6 0 2551 49 27123 714.6 0 27123 50 76167 714.6 0 76167 51 43964 714.6 0 43964 52 286 714.6 0 286 53 31 714.6 0 31 54 56 714.6 0 56 55 52 714.6 0 52 56 57 714.6 0 57 57 41 714.6 0 41 58 50 714.6 0 50 59 49 714.6 0 49 60 45 714.6 0 45 61 23 714.6 0 23 62 58 714.6 0 58 63 97 714.6 0 97 64 134 714.6 0 134 65 126 714.6 0 126 66 32 714.6 0 32 67 23 714.6 0 23 68 56 714.6 0 56 69 26 714.6 0 26 70 33 714.6 0 33 71 44 714.6 0 44 72 35 714.6 0 35 73 35 714.6 0 35 74 35 714.6 0 35 75 32 714.6 0 32 76 31 714.6 0 31 77 45 714.6 0 45 78 33 714.6 0 33 79 37 714.6 0 37 80 48 714.6 0 48 81 45 714.6 0 45 82 56 714.6 0 56 83 43 714.6 0 43 84 102 714.6 0 102 85 104 714.6 0 104 86 62 714.6 0 62 87 60 714.6 0 60 88 75 714.6 0 75 89 40 714.6 0 40 90 64 714.6 0 64 91 35 714.6 0 35 92 37 714.6 0 37 93 48 714.6 0 48 94 66 714.6 0 66 95 37 714.6 0 37 96 43 714.6 0 43 97 32 714.6 0 32 98 34 714.6 0 34 99 25 714.6 0 25 100 32 714.6 0 32 101 28 714.6 0 28 102 46 714.6 0 46 103 37 714.6 0 37 104 21 714.6 0 21 105 19 714.6 0 19 106 30 714.6 0 30 107 30 714.6 0 30 108 12 714.6 0 12 109 21 714.6 0 21 110 30 714.6 0 30 111 36 714.6 0 36 112 45 714.6 0 45 113 37 714.6 0 37 114 22 714.6 0 22 115 28 714.6 0 28 116 32 714.6 0 32 117 161 714.6 0 161 118 128 714.6 0 128 119 118 714.6 0 118 120 97 714.6 0 97 121 33 714.6 0 33 122 40 714.6 0 40 123 47 714.6 0 47 124 64 714.6 0 64 125 36 714.6 0 36 126 24 714.6 0 24 127 45 714.6 0 45 128 168 714.6 0 168 129 36 714.6 0 36 130 53 714.6 0 53 131 52 714.6 0 52 132 42 714.6 0 42 133 42 714.6 0 42 134 49 714.6 0 49 135 50 714.6 0 50 136 41 714.6 0 41 137 46 714.6 0 46 138 50 714.6 0 50 139 42 714.6 0 42 140 39 714.6 0 39 141 31 714.6 0 31 142 33 714.6 0 33 143 48 714.6 0 48 144 62 714.6 0 62 145 47 714.6 0 47 146 54 714.6 0 54 147 94 714.6 0 94 148 24 714.6 0 24 149 33 714.6 0 33 150 30 714.6 0 30 151 28 714.6 0 28 152 30 714.6 0 30 153 47 714.6 0 47 154 27 714.6 0 27 155 39 714.6 0 39 156 39 714.6 0 39 157 56 714.6 0 56 158 71 714.6 0 71 159 50 714.6 0 50 160 40 714.6 0 40 161 50 714.6 0 50 162 40 714.6 0 40 163 37 714.6 0 37 164 31 714.6 0 31 165 53 714.6 0 53 166 40 714.6 0 40 167 52 714.6 0 52 168 54 714.6 0 54 169 35 714.6 0 35 170 41 714.6 0 41 171 69 714.6 0 69 172 56 714.6 0 56 173 66 714.6 0 66 174 40 714.6 0 40 175 59 714.6 0 59 176 93 714.6 0 93 177 98 714.6 0 98 178 115 714.6 0 115 179 167 714.6 0 167 180 222 714.6 0 222 181 318 714.6 0 318 182 4650 714.6 0 4650 183 3736 714.6 0 3736 184 635 714.6 0 635 185 104 714.6 0 104 186 98 714.6 0 98 187 85 714.6 0 85 188 104 714.6 0 104 189 94 714.6 0 94 190 141 714.6 0 141 191 146 714.6 0 146 192 174 714.6 0 174 193 481 714.6 0 481 194 3190 714.6 0 3190 195 1495 714.6 0 1495 196 3665 714.6 0 3665 197 4081 714.6 0 4081 198 755 714.6 0 755 199 42 714.6 0 42 200 44 714.6 0 44 201 29 714.6 0 29 202 29 714.6 0 29 203 20 714.6 0 20 204 35 714.6 0 35 205 73 714.6 0 73 206 54 714.6 0 54 207 50 714.6 0 50 208 47 714.6 0 47 209 31 714.6 0 31 210 23 714.6 0 23 211 35 714.6 0 35 212 49 714.6 0 49 213 78 714.6 0 78 214 145 714.6 0 145 215 220 714.6 0 220 216 145 714.6 0 145 217 115 714.6 0 115 218 58 714.6 0 58 219 116 714.6 0 116 220 134 714.6 0 134 221 123 714.6 0 123 222 88 714.6 0 88 223 66 714.6 0 66 224 35 714.6 0 35 225 45 714.6 0 45 226 51 714.6 0 51 227 63 714.6 0 63 228 66 714.6 0 66 229 46 714.6 0 46 230 46 714.6 0 46 231 52 714.6 0 52 232 36 714.6 0 36 233 44 714.6 0 44 234 61 714.6 0 61 235 55 714.6 0 55 236 80 714.6 0 80 237 76 714.6 0 76 238 76 714.6 0 76 239 88 714.6 0 88 240 125 714.6 0 125 241 147 714.6 0 147 242 48 714.6 0 48 243 25 714.6 0 25 244 34 714.6 0 34 245 39 714.6 0 39 246 99 714.6 0 99 247 69 714.6 0 69 248 55 714.6 0 55 249 31 714.6 0 31 250 53 714.6 0 53 251 162 714.6 0 162 252 119 714.6 0 119 253 64 714.6 0 64 254 45 714.6 0 45 255 51 714.6 0 51 256 81 714.6 0 81 257 88 714.6 0 88 258 72 714.6 0 72 259 53 714.6 0 53 260 44 714.6 0 44 261 119 714.6 0 119 262 23 714.6 0 23 263 161 714.6 0 161 264 278 714.6 0 278 265 441 714.6 0 441 266 486 714.6 0 486 267 131 714.6 0 131 268 91 714.6 0 91 269 53 714.6 0 53 270 61 714.6 0 61 271 92 714.6 0 92 272 22 714.6 0 22 273 42 714.6 0 42 274 42 714.6 0 42 275 48 714.6 0 48 276 33 714.6 0 33 277 27 714.6 0 27 278 109 714.6 0 109 279 56 714.6 0 56 280 46 714.6 0 46 281 42 714.6 0 42 282 56 714.6 0 56 283 49 714.6 0 49 284 75 714.6 0 75 285 75 714.6 0 75 286 62 714.6 0 62 287 42 714.6 0 42 288 160 714.6 0 160 289 308 714.6 0 308 290 397 714.6 0 397 291 55 714.6 0 55 292 47 714.6 0 47 293 122 714.6 0 122 294 111 714.6 0 111 295 57 714.6 0 57 296 21 714.6 0 21 297 18 714.6 0 18 298 20 714.6 0 20 299 38 714.6 0 38 300 56 714.6 0 56 301 28 714.6 0 28 Finished in 10.38 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 254,344 Reads with adapters: 8,506 (3.3%) Reads that were too short: 1,236 (0.5%) Reads written (passing filters): 7,270 (2.9%) Total basepairs processed: 62,194,673 bp Total written (filtered): 1,652,968 bp (2.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8506 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 20 0.0 3 0 16 3 1 17 4822 0.0 3 4673 124 22 3 18 17 0.0 3 1 15 0 1 19 9 0.0 3 0 0 1 8 20 7 0.0 3 0 0 0 7 21 1 0.0 3 1 24 1 0.0 3 1 27 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 1 40 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 14 0.0 3 0 0 0 14 49 2 0.0 3 0 0 0 2 51 3 0.0 3 0 0 0 3 53 1 0.0 3 0 0 0 1 54 5 0.0 3 0 0 0 5 55 6 0.0 3 0 0 0 6 56 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 73 49 0.0 3 0 0 0 49 74 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 87 47 0.0 3 0 0 0 47 89 8 0.0 3 0 0 1 7 90 23 0.0 3 0 0 1 22 92 28 0.0 3 0 0 0 28 93 29 0.0 3 0 0 0 29 94 2 0.0 3 0 0 0 2 97 6 0.0 3 0 0 0 6 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 102 7 0.0 3 0 0 0 7 103 5 0.0 3 0 0 0 5 105 2 0.0 3 0 0 0 2 106 2 0.0 3 0 0 0 2 107 3 0.0 3 0 0 0 3 116 4 0.0 3 0 0 0 4 117 10 0.0 3 0 0 0 10 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 3 0.0 3 0 0 0 3 122 278 0.0 3 0 0 3 275 123 108 0.0 3 0 0 1 107 124 54 0.0 3 0 0 0 54 125 886 0.0 3 0 0 6 880 126 35 0.0 3 0 0 0 35 127 5 0.0 3 0 0 0 5 128 170 0.0 3 0 0 4 166 129 2 0.0 3 0 0 0 2 130 8 0.0 3 0 0 0 8 131 12 0.0 3 0 0 0 12 132 1 0.0 3 0 0 0 1 133 5 0.0 3 0 0 0 5 134 5 0.0 3 0 0 1 4 135 3 0.0 3 0 0 0 3 136 2 0.0 3 0 0 0 2 137 24 0.0 3 0 0 0 24 138 11 0.0 3 0 0 0 11 139 8 0.0 3 0 0 0 8 140 8 0.0 3 0 0 0 8 141 9 0.0 3 0 0 0 9 142 222 0.0 3 0 0 0 222 143 5 0.0 3 0 0 0 5 144 1 0.0 3 0 0 0 1 145 52 0.0 3 0 0 1 51 146 16 0.0 3 0 0 0 16 147 4 0.0 3 0 0 0 4 148 2 0.0 3 0 0 0 2 149 7 0.0 3 0 0 0 7 150 2 0.0 3 0 0 0 2 151 55 0.0 3 0 0 0 55 152 8 0.0 3 0 0 0 8 153 19 0.0 3 0 0 0 19 154 58 0.0 3 0 0 0 58 155 3 0.0 3 0 0 0 3 156 1 0.0 3 0 0 0 1 157 13 0.0 3 0 0 0 13 160 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 166 7 0.0 3 0 0 0 7 167 1 0.0 3 0 0 0 1 168 7 0.0 3 0 0 0 7 169 2 0.0 3 0 0 0 2 170 3 0.0 3 0 0 0 3 171 51 0.0 3 0 0 0 51 172 40 0.0 3 0 0 0 40 173 1 0.0 3 0 0 0 1 174 10 0.0 3 0 0 0 10 175 7 0.0 3 0 0 0 7 176 1 0.0 3 0 0 0 1 177 30 0.0 3 0 0 0 30 178 3 0.0 3 0 0 0 3 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 2 0.0 3 0 0 0 2 194 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 10 0.0 3 0 0 0 10 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 4 0.0 3 0 0 0 4 209 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 0 2 212 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 219 4 0.0 3 0 0 0 4 220 1 0.0 3 0 0 0 1 221 7 0.0 3 0 0 0 7 223 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 227 15 0.0 3 0 0 0 15 228 2 0.0 3 0 0 0 2 231 4 0.0 3 0 0 0 4 232 4 0.0 3 0 0 0 4 234 81 0.0 3 0 0 1 80 235 80 0.0 3 0 1 1 78 236 35 0.0 3 0 0 2 33 237 245 0.0 3 0 0 3 242 238 160 0.0 3 0 1 4 155 239 29 0.0 3 0 0 2 27 240 68 0.0 3 0 0 3 65 241 12 0.0 3 0 0 0 12 242 20 0.0 3 0 0 0 20 243 34 0.0 3 0 0 0 34 244 101 0.0 3 0 0 3 98 245 9 0.0 3 0 0 0 9 246 6 0.0 3 0 0 0 6 247 4 0.0 3 0 0 0 4 248 2 0.0 3 0 0 0 2 249 7 0.0 3 0 0 0 7 250 5 0.0 3 0 0 0 5 251 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 254 2 0.0 3 0 0 0 2 256 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 260 2 0.0 3 0 0 0 2 262 2 0.0 3 0 0 0 2 263 15 0.0 3 0 0 0 15 264 8 0.0 3 0 0 0 8 265 5 0.0 3 0 0 0 5 266 18 0.0 3 0 0 1 17 267 10 0.0 3 0 0 0 10 268 3 0.0 3 0 0 0 3 269 3 0.0 3 0 0 0 3 270 1 0.0 3 0 0 0 1 271 2 0.0 3 0 0 0 2 272 4 0.0 3 0 0 0 4 273 11 0.0 3 0 0 0 11 274 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 6 0.0 3 0 0 1 5 283 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 288 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.24 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 7,270 Reads with adapters: 391 (5.4%) Reads written (passing filters): 7,270 (100.0%) Total basepairs processed: 1,652,968 bp Total written (filtered): 1,651,795 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 391 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.0% C: 31.5% G: 28.6% T: 5.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 391 113.6 0 391