This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.84 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 269,832 (9.2%) Reads that were too short: 14,632 (0.5%) Reads written (passing filters): 255,200 (8.7%) Total basepairs processed: 881,011,047 bp Total written (filtered): 58,945,732 bp (6.7%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 269832 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 705 714.6 0 705 7 1950 714.6 0 1950 8 58632 714.6 0 58632 9 4438 714.6 0 4438 10 48 714.6 0 48 11 45 714.6 0 45 12 41 714.6 0 41 13 65 714.6 0 65 14 50 714.6 0 50 15 38 714.6 0 38 16 48 714.6 0 48 17 48 714.6 0 48 18 46 714.6 0 46 19 45 714.6 0 45 20 42 714.6 0 42 21 41 714.6 0 41 22 49 714.6 0 49 23 74 714.6 0 74 24 69 714.6 0 69 25 43 714.6 0 43 26 49 714.6 0 49 27 40 714.6 0 40 28 49 714.6 0 49 29 1161 714.6 0 1161 30 278 714.6 0 278 31 53 714.6 0 53 32 62 714.6 0 62 33 51 714.6 0 51 34 79 714.6 0 79 35 64 714.6 0 64 36 76 714.6 0 76 37 82 714.6 0 82 38 134 714.6 0 134 39 75 714.6 0 75 40 67 714.6 0 67 41 69 714.6 0 69 42 106 714.6 0 106 43 93 714.6 0 93 44 90 714.6 0 90 45 140 714.6 0 140 46 181 714.6 0 181 47 291 714.6 0 291 48 623 714.6 0 623 49 1439 714.6 0 1439 50 306 714.6 0 306 51 517 714.6 0 517 52 1236 714.6 0 1236 53 615 714.6 0 615 54 474 714.6 0 474 55 710 714.6 0 710 56 714 714.6 0 714 57 668 714.6 0 668 58 674 714.6 0 674 59 986 714.6 0 986 60 899 714.6 0 899 61 1706 714.6 0 1706 62 35670 714.6 0 35670 63 29442 714.6 0 29442 64 48545 714.6 0 48545 65 420 714.6 0 420 66 36 714.6 0 36 67 34 714.6 0 34 68 43 714.6 0 43 69 38 714.6 0 38 70 61 714.6 0 61 71 83 714.6 0 83 72 146 714.6 0 146 73 141 714.6 0 141 74 50 714.6 0 50 75 89 714.6 0 89 76 992 714.6 0 992 77 1146 714.6 0 1146 78 240 714.6 0 240 79 51 714.6 0 51 80 41 714.6 0 41 81 36 714.6 0 36 82 57 714.6 0 57 83 73 714.6 0 73 84 33 714.6 0 33 85 63 714.6 0 63 86 47 714.6 0 47 87 50 714.6 0 50 88 33 714.6 0 33 89 23 714.6 0 23 90 28 714.6 0 28 91 31 714.6 0 31 92 44 714.6 0 44 93 89 714.6 0 89 94 76 714.6 0 76 95 51 714.6 0 51 96 40 714.6 0 40 97 46 714.6 0 46 98 49 714.6 0 49 99 41 714.6 0 41 100 51 714.6 0 51 101 61 714.6 0 61 102 68 714.6 0 68 103 55 714.6 0 55 104 63 714.6 0 63 105 201 714.6 0 201 106 100 714.6 0 100 107 79 714.6 0 79 108 99 714.6 0 99 109 36 714.6 0 36 110 57 714.6 0 57 111 53 714.6 0 53 112 58 714.6 0 58 113 46 714.6 0 46 114 50 714.6 0 50 115 65 714.6 0 65 116 63 714.6 0 63 117 47 714.6 0 47 118 63 714.6 0 63 119 54 714.6 0 54 120 44 714.6 0 44 121 52 714.6 0 52 122 48 714.6 0 48 123 61 714.6 0 61 124 76 714.6 0 76 125 59 714.6 0 59 126 91 714.6 0 91 127 109 714.6 0 109 128 102 714.6 0 102 129 78 714.6 0 78 130 58 714.6 0 58 131 61 714.6 0 61 132 67 714.6 0 67 133 95 714.6 0 95 134 104 714.6 0 104 135 85 714.6 0 85 136 75 714.6 0 75 137 81 714.6 0 81 138 71 714.6 0 71 139 70 714.6 0 70 140 85 714.6 0 85 141 111 714.6 0 111 142 134 714.6 0 134 143 221 714.6 0 221 144 1771 714.6 0 1771 145 1536 714.6 0 1536 146 1257 714.6 0 1257 147 153 714.6 0 153 148 79 714.6 0 79 149 149 714.6 0 149 150 203 714.6 0 203 151 173 714.6 0 173 152 98 714.6 0 98 153 85 714.6 0 85 154 118 714.6 0 118 155 185 714.6 0 185 156 1098 714.6 0 1098 157 1183 714.6 0 1183 158 443 714.6 0 443 159 194 714.6 0 194 160 184 714.6 0 184 161 300 714.6 0 300 162 439 714.6 0 439 163 1099 714.6 0 1099 164 23383 714.6 0 23383 165 5534 714.6 0 5534 166 2494 714.6 0 2494 167 229 714.6 0 229 168 239 714.6 0 239 169 635 714.6 0 635 170 725 714.6 0 725 171 810 714.6 0 810 172 403 714.6 0 403 173 335 714.6 0 335 174 194 714.6 0 194 175 84 714.6 0 84 176 97 714.6 0 97 177 112 714.6 0 112 178 97 714.6 0 97 179 271 714.6 0 271 180 192 714.6 0 192 181 171 714.6 0 171 182 155 714.6 0 155 183 115 714.6 0 115 184 214 714.6 0 214 185 224 714.6 0 224 186 295 714.6 0 295 187 118 714.6 0 118 188 130 714.6 0 130 189 748 714.6 0 748 190 1161 714.6 0 1161 191 1190 714.6 0 1190 192 369 714.6 0 369 193 75 714.6 0 75 194 55 714.6 0 55 195 86 714.6 0 86 196 79 714.6 0 79 197 123 714.6 0 123 198 117 714.6 0 117 199 86 714.6 0 86 200 86 714.6 0 86 201 63 714.6 0 63 202 75 714.6 0 75 203 97 714.6 0 97 204 159 714.6 0 159 205 141 714.6 0 141 206 157 714.6 0 157 207 127 714.6 0 127 208 150 714.6 0 150 209 94 714.6 0 94 210 79 714.6 0 79 211 98 714.6 0 98 212 96 714.6 0 96 213 103 714.6 0 103 214 155 714.6 0 155 215 116 714.6 0 116 216 247 714.6 0 247 217 308 714.6 0 308 218 330 714.6 0 330 219 126 714.6 0 126 220 78 714.6 0 78 221 106 714.6 0 106 222 66 714.6 0 66 223 85 714.6 0 85 224 86 714.6 0 86 225 156 714.6 0 156 226 203 714.6 0 203 227 220 714.6 0 220 228 237 714.6 0 237 229 361 714.6 0 361 230 403 714.6 0 403 231 108 714.6 0 108 232 112 714.6 0 112 233 122 714.6 0 122 234 124 714.6 0 124 235 98 714.6 0 98 236 114 714.6 0 114 237 138 714.6 0 138 238 106 714.6 0 106 239 103 714.6 0 103 240 90 714.6 0 90 241 98 714.6 0 98 242 39 714.6 0 39 243 91 714.6 0 91 244 121 714.6 0 121 245 153 714.6 0 153 246 97 714.6 0 97 247 124 714.6 0 124 248 96 714.6 0 96 249 91 714.6 0 91 250 75 714.6 0 75 251 42 714.6 0 42 252 69 714.6 0 69 253 68 714.6 0 68 254 124 714.6 0 124 255 115 714.6 0 115 256 108 714.6 0 108 257 140 714.6 0 140 258 97 714.6 0 97 259 150 714.6 0 150 260 523 714.6 0 523 261 463 714.6 0 463 262 277 714.6 0 277 263 106 714.6 0 106 264 124 714.6 0 124 265 145 714.6 0 145 266 212 714.6 0 212 267 154 714.6 0 154 268 155 714.6 0 155 269 59 714.6 0 59 270 108 714.6 0 108 271 85 714.6 0 85 272 86 714.6 0 86 273 77 714.6 0 77 274 89 714.6 0 89 275 113 714.6 0 113 276 130 714.6 0 130 277 156 714.6 0 156 278 125 714.6 0 125 279 98 714.6 0 98 280 104 714.6 0 104 281 318 714.6 0 318 282 112 714.6 0 112 283 77 714.6 0 77 284 91 714.6 0 91 285 171 714.6 0 171 286 146 714.6 0 146 287 394 714.6 0 394 288 790 714.6 0 790 289 394 714.6 0 394 290 96 714.6 0 96 291 70 714.6 0 70 292 58 714.6 0 58 293 91 714.6 0 91 294 140 714.6 0 140 295 142 714.6 0 142 296 106 714.6 0 106 297 90 714.6 0 90 298 94 714.6 0 94 299 58 714.6 0 58 300 59 714.6 0 59 301 74 714.6 0 74 Finished in 11.29 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 255,200 Reads with adapters: 5,856 (2.3%) Reads that were too short: 49 (0.0%) Reads written (passing filters): 5,807 (2.3%) Total basepairs processed: 58,945,732 bp Total written (filtered): 1,458,756 bp (2.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5856 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 0 4 18 3 0.0 3 0 0 3 19 41 0.0 3 0 38 1 0 2 20 4604 0.0 4 4546 54 3 0 1 21 8 0.0 4 0 4 0 0 4 22 2 0.0 4 0 0 0 1 1 26 4 0.0 4 0 0 0 1 3 28 1 0.0 4 0 0 0 0 1 34 2 0.0 4 0 0 0 0 2 37 2 0.0 4 0 0 0 0 2 43 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 62 2 0.0 4 0 0 0 0 2 63 1 0.0 4 0 0 0 0 1 71 3 0.0 4 0 0 0 0 3 72 816 0.0 4 0 0 0 0 816 92 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 0 0 2 101 1 0.0 4 0 0 0 1 111 3 0.0 4 0 0 0 2 1 122 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 305 0.0 4 0 0 0 0 305 128 1 0.0 4 0 0 0 0 1 145 2 0.0 4 2 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 173 18 0.0 4 0 0 0 1 17 174 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 228 2 0.0 4 0 0 0 0 2 230 3 0.0 4 0 0 0 0 3 265 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.19 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 5,807 Reads with adapters: 122 (2.1%) Reads written (passing filters): 5,807 (100.0%) Total basepairs processed: 1,458,756 bp Total written (filtered): 1,455,572 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 122 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.8% C: 7.4% G: 81.1% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 90.7 0 2 4 7 22.7 0 7 5 1 5.7 0 1 6 3 1.4 0 3 9 1 0.0 0 0 1 10 64 0.0 1 5 59 11 34 0.0 1 2 32 12 1 0.0 1 0 1 223 5 0.0 1 5 224 1 0.0 1 1 226 1 0.0 1 1 257 1 0.0 1 0 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.