This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.66 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 134,142 (4.6%) Reads that were too short: 13,201 (0.5%) Reads written (passing filters): 120,941 (4.1%) Total basepairs processed: 881,011,047 bp Total written (filtered): 26,900,245 bp (3.1%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 134142 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1614 714.6 0 1614 7 18499 714.6 0 18499 8 189 714.6 0 189 9 4353 714.6 0 4353 10 48 714.6 0 48 11 48 714.6 0 48 12 29 714.6 0 29 13 41 714.6 0 41 14 40 714.6 0 40 15 52 714.6 0 52 16 43 714.6 0 43 17 42 714.6 0 42 18 45 714.6 0 45 19 33 714.6 0 33 20 31 714.6 0 31 21 37 714.6 0 37 22 47 714.6 0 47 23 31 714.6 0 31 24 63 714.6 0 63 25 43 714.6 0 43 26 41 714.6 0 41 27 52 714.6 0 52 28 47 714.6 0 47 29 38 714.6 0 38 30 52 714.6 0 52 31 52 714.6 0 52 32 60 714.6 0 60 33 37 714.6 0 37 34 52 714.6 0 52 35 55 714.6 0 55 36 58 714.6 0 58 37 40 714.6 0 40 38 45 714.6 0 45 39 34 714.6 0 34 40 54 714.6 0 54 41 48 714.6 0 48 42 38 714.6 0 38 43 32 714.6 0 32 44 33 714.6 0 33 45 75 714.6 0 75 46 45 714.6 0 45 47 49 714.6 0 49 48 42 714.6 0 42 49 43 714.6 0 43 50 42 714.6 0 42 51 43 714.6 0 43 52 40 714.6 0 40 53 50 714.6 0 50 54 51 714.6 0 51 55 59 714.6 0 59 56 43 714.6 0 43 57 63 714.6 0 63 58 32 714.6 0 32 59 38 714.6 0 38 60 43 714.6 0 43 61 42 714.6 0 42 62 86 714.6 0 86 63 58 714.6 0 58 64 47 714.6 0 47 65 57 714.6 0 57 66 83 714.6 0 83 67 53 714.6 0 53 68 85 714.6 0 85 69 87 714.6 0 87 70 156 714.6 0 156 71 189 714.6 0 189 72 450 714.6 0 450 73 1504 714.6 0 1504 74 392 714.6 0 392 75 400 714.6 0 400 76 791 714.6 0 791 77 363 714.6 0 363 78 409 714.6 0 409 79 658 714.6 0 658 80 646 714.6 0 646 81 623 714.6 0 623 82 686 714.6 0 686 83 615 714.6 0 615 84 808 714.6 0 808 85 1447 714.6 0 1447 86 14321 714.6 0 14321 87 24165 714.6 0 24165 88 25366 714.6 0 25366 89 199 714.6 0 199 90 51 714.6 0 51 91 112 714.6 0 112 92 83 714.6 0 83 93 125 714.6 0 125 94 143 714.6 0 143 95 120 714.6 0 120 96 67 714.6 0 67 97 68 714.6 0 68 98 85 714.6 0 85 99 123 714.6 0 123 100 198 714.6 0 198 101 127 714.6 0 127 102 58 714.6 0 58 103 47 714.6 0 47 104 30 714.6 0 30 105 33 714.6 0 33 106 32 714.6 0 32 107 27 714.6 0 27 108 70 714.6 0 70 109 96 714.6 0 96 110 100 714.6 0 100 111 85 714.6 0 85 112 95 714.6 0 95 113 47 714.6 0 47 114 55 714.6 0 55 115 116 714.6 0 116 116 110 714.6 0 110 117 98 714.6 0 98 118 108 714.6 0 108 119 107 714.6 0 107 120 111 714.6 0 111 121 117 714.6 0 117 122 107 714.6 0 107 123 130 714.6 0 130 124 219 714.6 0 219 125 837 714.6 0 837 126 224 714.6 0 224 127 872 714.6 0 872 128 128 714.6 0 128 129 144 714.6 0 144 130 153 714.6 0 153 131 186 714.6 0 186 132 117 714.6 0 117 133 100 714.6 0 100 134 152 714.6 0 152 135 127 714.6 0 127 136 157 714.6 0 157 137 143 714.6 0 143 138 146 714.6 0 146 139 142 714.6 0 142 140 109 714.6 0 109 141 184 714.6 0 184 142 171 714.6 0 171 143 152 714.6 0 152 144 396 714.6 0 396 145 1287 714.6 0 1287 146 2240 714.6 0 2240 147 462 714.6 0 462 148 72 714.6 0 72 149 94 714.6 0 94 150 64 714.6 0 64 151 85 714.6 0 85 152 90 714.6 0 90 153 82 714.6 0 82 154 127 714.6 0 127 155 156 714.6 0 156 156 163 714.6 0 163 157 128 714.6 0 128 158 89 714.6 0 89 159 81 714.6 0 81 160 93 714.6 0 93 161 89 714.6 0 89 162 65 714.6 0 65 163 85 714.6 0 85 164 78 714.6 0 78 165 128 714.6 0 128 166 514 714.6 0 514 167 793 714.6 0 793 168 143 714.6 0 143 169 89 714.6 0 89 170 104 714.6 0 104 171 91 714.6 0 91 172 110 714.6 0 110 173 103 714.6 0 103 174 106 714.6 0 106 175 128 714.6 0 128 176 122 714.6 0 122 177 97 714.6 0 97 178 106 714.6 0 106 179 89 714.6 0 89 180 122 714.6 0 122 181 100 714.6 0 100 182 107 714.6 0 107 183 103 714.6 0 103 184 103 714.6 0 103 185 129 714.6 0 129 186 352 714.6 0 352 187 466 714.6 0 466 188 96 714.6 0 96 189 66 714.6 0 66 190 98 714.6 0 98 191 135 714.6 0 135 192 103 714.6 0 103 193 138 714.6 0 138 194 104 714.6 0 104 195 97 714.6 0 97 196 87 714.6 0 87 197 124 714.6 0 124 198 138 714.6 0 138 199 77 714.6 0 77 200 67 714.6 0 67 201 96 714.6 0 96 202 121 714.6 0 121 203 141 714.6 0 141 204 185 714.6 0 185 205 244 714.6 0 244 206 320 714.6 0 320 207 1019 714.6 0 1019 208 418 714.6 0 418 209 95 714.6 0 95 210 122 714.6 0 122 211 375 714.6 0 375 212 624 714.6 0 624 213 296 714.6 0 296 214 100 714.6 0 100 215 74 714.6 0 74 216 75 714.6 0 75 217 105 714.6 0 105 218 90 714.6 0 90 219 90 714.6 0 90 220 68 714.6 0 68 221 75 714.6 0 75 222 106 714.6 0 106 223 119 714.6 0 119 224 101 714.6 0 101 225 105 714.6 0 105 226 94 714.6 0 94 227 174 714.6 0 174 228 87 714.6 0 87 229 100 714.6 0 100 230 54 714.6 0 54 231 75 714.6 0 75 232 79 714.6 0 79 233 83 714.6 0 83 234 97 714.6 0 97 235 73 714.6 0 73 236 71 714.6 0 71 237 65 714.6 0 65 238 51 714.6 0 51 239 79 714.6 0 79 240 44 714.6 0 44 241 315 714.6 0 315 242 328 714.6 0 328 243 821 714.6 0 821 244 83 714.6 0 83 245 81 714.6 0 81 246 67 714.6 0 67 247 38 714.6 0 38 248 31 714.6 0 31 249 32 714.6 0 32 250 76 714.6 0 76 251 141 714.6 0 141 252 188 714.6 0 188 253 126 714.6 0 126 254 75 714.6 0 75 255 90 714.6 0 90 256 64 714.6 0 64 257 47 714.6 0 47 258 25 714.6 0 25 259 37 714.6 0 37 260 53 714.6 0 53 261 87 714.6 0 87 262 106 714.6 0 106 263 164 714.6 0 164 264 139 714.6 0 139 265 127 714.6 0 127 266 83 714.6 0 83 267 71 714.6 0 71 268 39 714.6 0 39 269 34 714.6 0 34 270 48 714.6 0 48 271 66 714.6 0 66 272 102 714.6 0 102 273 62 714.6 0 62 274 51 714.6 0 51 275 62 714.6 0 62 276 56 714.6 0 56 277 159 714.6 0 159 278 171 714.6 0 171 279 113 714.6 0 113 280 51 714.6 0 51 281 37 714.6 0 37 282 86 714.6 0 86 283 80 714.6 0 80 284 95 714.6 0 95 285 67 714.6 0 67 286 39 714.6 0 39 287 42 714.6 0 42 288 31 714.6 0 31 289 59 714.6 0 59 290 102 714.6 0 102 291 148 714.6 0 148 292 77 714.6 0 77 293 45 714.6 0 45 294 43 714.6 0 43 295 98 714.6 0 98 296 123 714.6 0 123 297 647 714.6 0 647 298 157 714.6 0 157 299 86 714.6 0 86 300 78 714.6 0 78 301 258 714.6 0 258 Finished in 4.87 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 120,941 Reads with adapters: 5,858 (4.8%) Reads that were too short: 127 (0.1%) Reads written (passing filters): 5,731 (4.7%) Total basepairs processed: 26,900,245 bp Total written (filtered): 1,488,451 bp (5.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5858 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 28 0.0 3 0 24 3 1 17 4383 0.0 3 4270 95 14 4 18 26 0.0 3 4 17 5 19 742 0.0 3 706 28 6 2 20 6 0.0 3 1 3 1 1 21 7 0.0 3 0 0 0 7 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 1 1 43 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 66 20 0.0 3 0 0 0 20 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 80 16 0.0 3 0 0 0 16 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 4 0.0 3 0 0 0 4 88 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 100 5 0.0 3 0 0 0 5 102 2 0.0 3 0 0 0 2 104 7 0.0 3 0 0 0 7 105 369 0.0 3 0 0 1 368 106 19 0.0 3 0 0 0 19 107 1 0.0 3 0 0 0 1 108 67 0.0 3 0 0 2 65 109 5 0.0 3 0 0 0 5 112 1 0.0 3 0 0 0 1 117 1 0.0 3 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 132 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 158 4 0.0 3 0 0 0 4 161 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 166 25 0.0 3 0 0 0 25 167 3 0.0 3 0 0 0 3 168 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 170 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 6 0.0 3 0 0 0 6 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 193 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 205 7 0.0 3 0 0 0 7 206 26 0.0 3 0 0 0 26 207 13 0.0 3 0 0 0 13 208 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.20 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 5,731 Reads with adapters: 218 (3.8%) Reads written (passing filters): 5,731 (100.0%) Total basepairs processed: 1,488,451 bp Total written (filtered): 1,487,780 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 218 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 57.3% C: 19.7% G: 17.0% T: 6.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 206 89.5 0 206 4 8 22.4 0 8 5 3 5.6 0 3 6 1 1.4 0 1