This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.57 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 53,152 (1.8%) Reads that were too short: 8,193 (0.3%) Reads written (passing filters): 44,959 (1.5%) Total basepairs processed: 881,011,047 bp Total written (filtered): 8,037,870 bp (0.9%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 53152 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1019 714.6 0 1019 7 2896 714.6 0 2896 8 4546 714.6 0 4546 9 61 714.6 0 61 10 41 714.6 0 41 11 48 714.6 0 48 12 54 714.6 0 54 13 36 714.6 0 36 14 39 714.6 0 39 15 48 714.6 0 48 16 45 714.6 0 45 17 43 714.6 0 43 18 60 714.6 0 60 19 83 714.6 0 83 20 69 714.6 0 69 21 54 714.6 0 54 22 43 714.6 0 43 23 52 714.6 0 52 24 54 714.6 0 54 25 31 714.6 0 31 26 71 714.6 0 71 27 75 714.6 0 75 28 42 714.6 0 42 29 48 714.6 0 48 30 52 714.6 0 52 31 48 714.6 0 48 32 59 714.6 0 59 33 54 714.6 0 54 34 56 714.6 0 56 35 66 714.6 0 66 36 59 714.6 0 59 37 49 714.6 0 49 38 76 714.6 0 76 39 70 714.6 0 70 40 272 714.6 0 272 41 60 714.6 0 60 42 44 714.6 0 44 43 56 714.6 0 56 44 62 714.6 0 62 45 74 714.6 0 74 46 68 714.6 0 68 47 46 714.6 0 46 48 43 714.6 0 43 49 44 714.6 0 44 50 53 714.6 0 53 51 45 714.6 0 45 52 36 714.6 0 36 53 64 714.6 0 64 54 46 714.6 0 46 55 36 714.6 0 36 56 38 714.6 0 38 57 48 714.6 0 48 58 54 714.6 0 54 59 55 714.6 0 55 60 104 714.6 0 104 61 44 714.6 0 44 62 50 714.6 0 50 63 50 714.6 0 50 64 42 714.6 0 42 65 60 714.6 0 60 66 34 714.6 0 34 67 39 714.6 0 39 68 57 714.6 0 57 69 52 714.6 0 52 70 44 714.6 0 44 71 55 714.6 0 55 72 39 714.6 0 39 73 54 714.6 0 54 74 50 714.6 0 50 75 34 714.6 0 34 76 77 714.6 0 77 77 156 714.6 0 156 78 101 714.6 0 101 79 50 714.6 0 50 80 44 714.6 0 44 81 32 714.6 0 32 82 39 714.6 0 39 83 47 714.6 0 47 84 50 714.6 0 50 85 46 714.6 0 46 86 32 714.6 0 32 87 33 714.6 0 33 88 32 714.6 0 32 89 35 714.6 0 35 90 54 714.6 0 54 91 44 714.6 0 44 92 43 714.6 0 43 93 78 714.6 0 78 94 163 714.6 0 163 95 87 714.6 0 87 96 179 714.6 0 179 97 296 714.6 0 296 98 288 714.6 0 288 99 55 714.6 0 55 100 56 714.6 0 56 101 34 714.6 0 34 102 38 714.6 0 38 103 33 714.6 0 33 104 32 714.6 0 32 105 42 714.6 0 42 106 33 714.6 0 33 107 40 714.6 0 40 108 37 714.6 0 37 109 27 714.6 0 27 110 36 714.6 0 36 111 44 714.6 0 44 112 32 714.6 0 32 113 38 714.6 0 38 114 79 714.6 0 79 115 45 714.6 0 45 116 36 714.6 0 36 117 47 714.6 0 47 118 29 714.6 0 29 119 24 714.6 0 24 120 35 714.6 0 35 121 21 714.6 0 21 122 46 714.6 0 46 123 47 714.6 0 47 124 20 714.6 0 20 125 33 714.6 0 33 126 45 714.6 0 45 127 29 714.6 0 29 128 77 714.6 0 77 129 48 714.6 0 48 130 258 714.6 0 258 131 239 714.6 0 239 132 182 714.6 0 182 133 42 714.6 0 42 134 43 714.6 0 43 135 64 714.6 0 64 136 48 714.6 0 48 137 72 714.6 0 72 138 58 714.6 0 58 139 29 714.6 0 29 140 51 714.6 0 51 141 28 714.6 0 28 142 63 714.6 0 63 143 64 714.6 0 64 144 121 714.6 0 121 145 43 714.6 0 43 146 35 714.6 0 35 147 34 714.6 0 34 148 25 714.6 0 25 149 30 714.6 0 30 150 44 714.6 0 44 151 54 714.6 0 54 152 60 714.6 0 60 153 56 714.6 0 56 154 67 714.6 0 67 155 46 714.6 0 46 156 35 714.6 0 35 157 57 714.6 0 57 158 68 714.6 0 68 159 58 714.6 0 58 160 42 714.6 0 42 161 43 714.6 0 43 162 85 714.6 0 85 163 67 714.6 0 67 164 111 714.6 0 111 165 128 714.6 0 128 166 204 714.6 0 204 167 288 714.6 0 288 168 895 714.6 0 895 169 20913 714.6 0 20913 170 2230 714.6 0 2230 171 1027 714.6 0 1027 172 38 714.6 0 38 173 47 714.6 0 47 174 38 714.6 0 38 175 49 714.6 0 49 176 33 714.6 0 33 177 26 714.6 0 26 178 40 714.6 0 40 179 46 714.6 0 46 180 58 714.6 0 58 181 32 714.6 0 32 182 50 714.6 0 50 183 27 714.6 0 27 184 41 714.6 0 41 185 55 714.6 0 55 186 52 714.6 0 52 187 27 714.6 0 27 188 31 714.6 0 31 189 32 714.6 0 32 190 37 714.6 0 37 191 43 714.6 0 43 192 80 714.6 0 80 193 154 714.6 0 154 194 85 714.6 0 85 195 52 714.6 0 52 196 25 714.6 0 25 197 50 714.6 0 50 198 47 714.6 0 47 199 26 714.6 0 26 200 42 714.6 0 42 201 46 714.6 0 46 202 46 714.6 0 46 203 143 714.6 0 143 204 111 714.6 0 111 205 87 714.6 0 87 206 61 714.6 0 61 207 29 714.6 0 29 208 30 714.6 0 30 209 29 714.6 0 29 210 42 714.6 0 42 211 44 714.6 0 44 212 46 714.6 0 46 213 55 714.6 0 55 214 37 714.6 0 37 215 71 714.6 0 71 216 48 714.6 0 48 217 61 714.6 0 61 218 47 714.6 0 47 219 38 714.6 0 38 220 39 714.6 0 39 221 33 714.6 0 33 222 56 714.6 0 56 223 62 714.6 0 62 224 92 714.6 0 92 225 45 714.6 0 45 226 36 714.6 0 36 227 67 714.6 0 67 228 41 714.6 0 41 229 40 714.6 0 40 230 46 714.6 0 46 231 70 714.6 0 70 232 51 714.6 0 51 233 66 714.6 0 66 234 44 714.6 0 44 235 78 714.6 0 78 236 96 714.6 0 96 237 150 714.6 0 150 238 82 714.6 0 82 239 68 714.6 0 68 240 42 714.6 0 42 241 159 714.6 0 159 242 41 714.6 0 41 243 37 714.6 0 37 244 40 714.6 0 40 245 43 714.6 0 43 246 53 714.6 0 53 247 36 714.6 0 36 248 27 714.6 0 27 249 32 714.6 0 32 250 34 714.6 0 34 251 39 714.6 0 39 252 35 714.6 0 35 253 43 714.6 0 43 254 55 714.6 0 55 255 59 714.6 0 59 256 43 714.6 0 43 257 42 714.6 0 42 258 103 714.6 0 103 259 47 714.6 0 47 260 39 714.6 0 39 261 86 714.6 0 86 262 69 714.6 0 69 263 53 714.6 0 53 264 36 714.6 0 36 265 54 714.6 0 54 266 54 714.6 0 54 267 65 714.6 0 65 268 44 714.6 0 44 269 72 714.6 0 72 270 100 714.6 0 100 271 299 714.6 0 299 272 574 714.6 0 574 273 547 714.6 0 547 274 1664 714.6 0 1664 275 49 714.6 0 49 276 42 714.6 0 42 277 41 714.6 0 41 278 52 714.6 0 52 279 36 714.6 0 36 280 33 714.6 0 33 281 45 714.6 0 45 282 36 714.6 0 36 283 53 714.6 0 53 284 43 714.6 0 43 285 52 714.6 0 52 286 43 714.6 0 43 287 37 714.6 0 37 288 53 714.6 0 53 289 40 714.6 0 40 290 42 714.6 0 42 291 36 714.6 0 36 292 61 714.6 0 61 293 39 714.6 0 39 294 44 714.6 0 44 295 37 714.6 0 37 296 40 714.6 0 40 297 65 714.6 0 65 298 32 714.6 0 32 299 28 714.6 0 28 300 25 714.6 0 25 301 36 714.6 0 36 Finished in 1.74 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 44,959 Reads with adapters: 4,820 (10.7%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 4,817 (10.7%) Total basepairs processed: 8,037,870 bp Total written (filtered): 1,314,678 bp (16.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4820 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 65 0.0 3 0 0 0 0 65 18 2 0.0 3 0 0 2 19 36 0.0 3 0 35 1 20 4577 0.0 4 4533 44 21 11 0.0 4 2 8 0 0 1 22 3 0.0 4 0 0 1 0 2 23 113 0.0 4 110 1 0 2 24 3 0.0 4 0 0 0 0 3 93 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 1 111 2 0.0 4 0 0 0 2 130 2 0.0 4 0 0 0 0 2 184 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.16 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 4,817 Reads with adapters: 2,618 (54.3%) Reads written (passing filters): 4,817 (100.0%) Total basepairs processed: 1,314,678 bp Total written (filtered): 625,628 bp (47.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2618 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 96.8% C: 0.2% G: 3.0% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 10 1 0.0 1 0 1 11 78 0.0 1 1 77 257 1 0.0 1 0 1 270 7 0.0 1 6 1 271 2421 0.0 1 2342 79 272 86 0.0 1 85 1 273 24 0.0 1 24 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.