This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 79.33 s (27 us/read; 2.21 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 125,363 (4.3%) Reads that were too short: 27,168 (0.9%) Reads written (passing filters): 98,195 (3.4%) Total basepairs processed: 881,011,047 bp Total written (filtered): 24,299,044 bp (2.8%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 125363 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 471 714.6 0 471 7 11921 714.6 0 11921 8 41744 714.6 0 41744 9 85 714.6 0 85 10 32 714.6 0 32 11 31 714.6 0 31 12 34 714.6 0 34 13 31 714.6 0 31 14 26 714.6 0 26 15 26 714.6 0 26 16 31 714.6 0 31 17 33 714.6 0 33 18 96 714.6 0 96 19 30 714.6 0 30 20 70 714.6 0 70 21 38 714.6 0 38 22 56 714.6 0 56 23 26 714.6 0 26 24 22 714.6 0 22 25 22 714.6 0 22 26 30 714.6 0 30 27 41 714.6 0 41 28 27 714.6 0 27 29 6815 714.6 0 6815 30 358 714.6 0 358 31 2474 714.6 0 2474 32 245 714.6 0 245 33 25 714.6 0 25 34 19 714.6 0 19 35 24 714.6 0 24 36 30 714.6 0 30 37 31 714.6 0 31 38 29 714.6 0 29 39 28 714.6 0 28 40 30 714.6 0 30 41 30 714.6 0 30 42 21 714.6 0 21 43 38 714.6 0 38 44 35 714.6 0 35 45 36 714.6 0 36 46 41 714.6 0 41 47 40 714.6 0 40 48 31 714.6 0 31 49 32 714.6 0 32 50 35 714.6 0 35 51 48 714.6 0 48 52 69 714.6 0 69 53 20 714.6 0 20 54 54 714.6 0 54 55 40 714.6 0 40 56 85 714.6 0 85 57 14 714.6 0 14 58 29 714.6 0 29 59 29 714.6 0 29 60 31 714.6 0 31 61 88 714.6 0 88 62 128 714.6 0 128 63 62 714.6 0 62 64 32 714.6 0 32 65 26 714.6 0 26 66 17 714.6 0 17 67 63 714.6 0 63 68 62 714.6 0 62 69 32 714.6 0 32 70 25 714.6 0 25 71 53 714.6 0 53 72 90 714.6 0 90 73 82 714.6 0 82 74 192 714.6 0 192 75 88 714.6 0 88 76 303 714.6 0 303 77 299 714.6 0 299 78 291 714.6 0 291 79 213 714.6 0 213 80 221 714.6 0 221 81 205 714.6 0 205 82 212 714.6 0 212 83 313 714.6 0 313 84 260 714.6 0 260 85 175 714.6 0 175 86 213 714.6 0 213 87 480 714.6 0 480 88 92 714.6 0 92 89 186 714.6 0 186 90 156 714.6 0 156 91 163 714.6 0 163 92 351 714.6 0 351 93 342 714.6 0 342 94 335 714.6 0 335 95 339 714.6 0 339 96 300 714.6 0 300 97 177 714.6 0 177 98 405 714.6 0 405 99 408 714.6 0 408 100 402 714.6 0 402 101 140 714.6 0 140 102 75 714.6 0 75 103 48 714.6 0 48 104 25 714.6 0 25 105 21 714.6 0 21 106 29 714.6 0 29 107 34 714.6 0 34 108 124 714.6 0 124 109 154 714.6 0 154 110 201 714.6 0 201 111 231 714.6 0 231 112 233 714.6 0 233 113 254 714.6 0 254 114 178 714.6 0 178 115 151 714.6 0 151 116 149 714.6 0 149 117 120 714.6 0 120 118 127 714.6 0 127 119 174 714.6 0 174 120 204 714.6 0 204 121 188 714.6 0 188 122 174 714.6 0 174 123 195 714.6 0 195 124 272 714.6 0 272 125 240 714.6 0 240 126 274 714.6 0 274 127 736 714.6 0 736 128 862 714.6 0 862 129 633 714.6 0 633 130 631 714.6 0 631 131 346 714.6 0 346 132 212 714.6 0 212 133 210 714.6 0 210 134 215 714.6 0 215 135 294 714.6 0 294 136 287 714.6 0 287 137 194 714.6 0 194 138 187 714.6 0 187 139 184 714.6 0 184 140 169 714.6 0 169 141 163 714.6 0 163 142 202 714.6 0 202 143 184 714.6 0 184 144 252 714.6 0 252 145 210 714.6 0 210 146 238 714.6 0 238 147 236 714.6 0 236 148 371 714.6 0 371 149 171 714.6 0 171 150 143 714.6 0 143 151 117 714.6 0 117 152 225 714.6 0 225 153 239 714.6 0 239 154 264 714.6 0 264 155 287 714.6 0 287 156 228 714.6 0 228 157 215 714.6 0 215 158 222 714.6 0 222 159 206 714.6 0 206 160 208 714.6 0 208 161 218 714.6 0 218 162 198 714.6 0 198 163 182 714.6 0 182 164 185 714.6 0 185 165 270 714.6 0 270 166 377 714.6 0 377 167 369 714.6 0 369 168 284 714.6 0 284 169 198 714.6 0 198 170 167 714.6 0 167 171 148 714.6 0 148 172 208 714.6 0 208 173 182 714.6 0 182 174 309 714.6 0 309 175 305 714.6 0 305 176 252 714.6 0 252 177 241 714.6 0 241 178 256 714.6 0 256 179 220 714.6 0 220 180 250 714.6 0 250 181 223 714.6 0 223 182 254 714.6 0 254 183 313 714.6 0 313 184 284 714.6 0 284 185 276 714.6 0 276 186 222 714.6 0 222 187 191 714.6 0 191 188 164 714.6 0 164 189 184 714.6 0 184 190 301 714.6 0 301 191 375 714.6 0 375 192 409 714.6 0 409 193 269 714.6 0 269 194 246 714.6 0 246 195 279 714.6 0 279 196 424 714.6 0 424 197 568 714.6 0 568 198 623 714.6 0 623 199 186 714.6 0 186 200 216 714.6 0 216 201 143 714.6 0 143 202 336 714.6 0 336 203 410 714.6 0 410 204 321 714.6 0 321 205 292 714.6 0 292 206 247 714.6 0 247 207 247 714.6 0 247 208 220 714.6 0 220 209 215 714.6 0 215 210 384 714.6 0 384 211 593 714.6 0 593 212 566 714.6 0 566 213 416 714.6 0 416 214 297 714.6 0 297 215 268 714.6 0 268 216 279 714.6 0 279 217 274 714.6 0 274 218 356 714.6 0 356 219 340 714.6 0 340 220 321 714.6 0 321 221 292 714.6 0 292 222 344 714.6 0 344 223 413 714.6 0 413 224 319 714.6 0 319 225 248 714.6 0 248 226 287 714.6 0 287 227 193 714.6 0 193 228 177 714.6 0 177 229 199 714.6 0 199 230 165 714.6 0 165 231 194 714.6 0 194 232 271 714.6 0 271 233 316 714.6 0 316 234 383 714.6 0 383 235 315 714.6 0 315 236 223 714.6 0 223 237 185 714.6 0 185 238 261 714.6 0 261 239 310 714.6 0 310 240 422 714.6 0 422 241 328 714.6 0 328 242 676 714.6 0 676 243 461 714.6 0 461 244 371 714.6 0 371 245 467 714.6 0 467 246 520 714.6 0 520 247 294 714.6 0 294 248 41 714.6 0 41 249 113 714.6 0 113 250 204 714.6 0 204 251 853 714.6 0 853 252 761 714.6 0 761 253 536 714.6 0 536 254 303 714.6 0 303 255 347 714.6 0 347 256 232 714.6 0 232 257 117 714.6 0 117 258 77 714.6 0 77 259 70 714.6 0 70 260 85 714.6 0 85 261 147 714.6 0 147 262 309 714.6 0 309 263 576 714.6 0 576 264 498 714.6 0 498 265 366 714.6 0 366 266 205 714.6 0 205 267 150 714.6 0 150 268 109 714.6 0 109 269 119 714.6 0 119 270 138 714.6 0 138 271 279 714.6 0 279 272 388 714.6 0 388 273 355 714.6 0 355 274 206 714.6 0 206 275 83 714.6 0 83 276 119 714.6 0 119 277 224 714.6 0 224 278 415 714.6 0 415 279 270 714.6 0 270 280 95 714.6 0 95 281 89 714.6 0 89 282 142 714.6 0 142 283 278 714.6 0 278 284 348 714.6 0 348 285 157 714.6 0 157 286 124 714.6 0 124 287 67 714.6 0 67 288 66 714.6 0 66 289 123 714.6 0 123 290 278 714.6 0 278 291 353 714.6 0 353 292 261 714.6 0 261 293 140 714.6 0 140 294 106 714.6 0 106 295 90 714.6 0 90 296 101 714.6 0 101 297 134 714.6 0 134 298 129 714.6 0 129 299 137 714.6 0 137 300 158 714.6 0 158 301 81 714.6 0 81 Finished in 4.40 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 98,195 Reads with adapters: 10,991 (11.2%) Reads that were too short: 49 (0.0%) Reads written (passing filters): 10,942 (11.1%) Total basepairs processed: 24,299,044 bp Total written (filtered): 3,022,511 bp (12.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10991 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 16 85 0.0 3 0 78 5 2 17 10741 0.0 3 10400 288 49 4 18 36 0.0 3 1 26 6 3 19 13 0.0 3 0 1 8 4 20 13 0.0 3 0 0 0 13 32 1 0.0 3 1 33 1 0.0 3 0 0 0 1 42 3 0.0 3 0 0 0 3 44 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 1 63 11 0.0 3 0 0 0 11 66 10 0.0 3 0 0 0 10 80 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 1 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 4 0.0 3 0 0 0 4 167 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 0 1 183 4 0.0 3 0 0 1 3 187 1 0.0 3 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 194 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 221 5 0.0 3 0 0 0 5 259 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 3 0.0 3 0 0 0 3 281 6 0.0 3 0 0 0 6 282 5 0.0 3 0 0 0 5 283 1 0.0 3 0 0 0 1 288 3 0.0 3 0 0 0 3 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.40 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 10,942 Reads with adapters: 729 (6.7%) Reads written (passing filters): 10,942 (100.0%) Total basepairs processed: 3,022,511 bp Total written (filtered): 3,020,316 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 729 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.6% C: 47.5% G: 10.2% T: 7.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 725 171.0 0 725 5 4 10.7 0 4