This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.49 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 38,092 (1.3%) Reads that were too short: 2,995 (0.1%) Reads written (passing filters): 35,097 (1.2%) Total basepairs processed: 881,011,047 bp Total written (filtered): 9,538,919 bp (1.1%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 38092 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1990 714.6 0 1990 7 1737 714.6 0 1737 8 13598 714.6 0 13598 9 10565 714.6 0 10565 10 55 714.6 0 55 11 35 714.6 0 35 12 35 714.6 0 35 13 35 714.6 0 35 14 34 714.6 0 34 15 43 714.6 0 43 16 34 714.6 0 34 17 36 714.6 0 36 18 40 714.6 0 40 19 36 714.6 0 36 20 32 714.6 0 32 21 26 714.6 0 26 22 47 714.6 0 47 23 35 714.6 0 35 24 31 714.6 0 31 25 38 714.6 0 38 26 35 714.6 0 35 27 39 714.6 0 39 28 40 714.6 0 40 29 33 714.6 0 33 30 44 714.6 0 44 31 36 714.6 0 36 32 29 714.6 0 29 33 39 714.6 0 39 34 26 714.6 0 26 35 34 714.6 0 34 36 31 714.6 0 31 37 36 714.6 0 36 38 34 714.6 0 34 39 38 714.6 0 38 40 26 714.6 0 26 41 36 714.6 0 36 42 37 714.6 0 37 43 33 714.6 0 33 44 29 714.6 0 29 45 30 714.6 0 30 46 27 714.6 0 27 47 34 714.6 0 34 48 93 714.6 0 93 49 60 714.6 0 60 50 208 714.6 0 208 51 37 714.6 0 37 52 29 714.6 0 29 53 32 714.6 0 32 54 23 714.6 0 23 55 21 714.6 0 21 56 28 714.6 0 28 57 21 714.6 0 21 58 21 714.6 0 21 59 20 714.6 0 20 60 26 714.6 0 26 61 31 714.6 0 31 62 21 714.6 0 21 63 66 714.6 0 66 64 27 714.6 0 27 65 24 714.6 0 24 66 18 714.6 0 18 67 19 714.6 0 19 68 21 714.6 0 21 69 8 714.6 0 8 70 22 714.6 0 22 71 23 714.6 0 23 72 17 714.6 0 17 73 16 714.6 0 16 74 22 714.6 0 22 75 17 714.6 0 17 76 15 714.6 0 15 77 15 714.6 0 15 78 23 714.6 0 23 79 10 714.6 0 10 80 17 714.6 0 17 81 21 714.6 0 21 82 29 714.6 0 29 83 29 714.6 0 29 84 28 714.6 0 28 85 38 714.6 0 38 86 19 714.6 0 19 87 10 714.6 0 10 88 15 714.6 0 15 89 23 714.6 0 23 90 18 714.6 0 18 91 12 714.6 0 12 92 13 714.6 0 13 93 12 714.6 0 12 94 20 714.6 0 20 95 31 714.6 0 31 96 106 714.6 0 106 97 23 714.6 0 23 98 24 714.6 0 24 99 16 714.6 0 16 100 15 714.6 0 15 101 18 714.6 0 18 102 27 714.6 0 27 103 18 714.6 0 18 104 12 714.6 0 12 105 10 714.6 0 10 106 7 714.6 0 7 107 7 714.6 0 7 108 7 714.6 0 7 109 14 714.6 0 14 110 9 714.6 0 9 111 11 714.6 0 11 112 10 714.6 0 10 113 17 714.6 0 17 114 18 714.6 0 18 115 10 714.6 0 10 116 10 714.6 0 10 117 12 714.6 0 12 118 30 714.6 0 30 119 31 714.6 0 31 120 27 714.6 0 27 121 14 714.6 0 14 122 20 714.6 0 20 123 14 714.6 0 14 124 17 714.6 0 17 125 10 714.6 0 10 126 17 714.6 0 17 127 48 714.6 0 48 128 18 714.6 0 18 129 19 714.6 0 19 130 17 714.6 0 17 131 9 714.6 0 9 132 21 714.6 0 21 133 47 714.6 0 47 134 25 714.6 0 25 135 23 714.6 0 23 136 23 714.6 0 23 137 26 714.6 0 26 138 25 714.6 0 25 139 29 714.6 0 29 140 22 714.6 0 22 141 38 714.6 0 38 142 26 714.6 0 26 143 36 714.6 0 36 144 27 714.6 0 27 145 46 714.6 0 46 146 336 714.6 0 336 147 1828 714.6 0 1828 148 92 714.6 0 92 149 22 714.6 0 22 150 21 714.6 0 21 151 11 714.6 0 11 152 12 714.6 0 12 153 15 714.6 0 15 154 8 714.6 0 8 155 15 714.6 0 15 156 14 714.6 0 14 157 17 714.6 0 17 158 16 714.6 0 16 159 19 714.6 0 19 160 13 714.6 0 13 161 14 714.6 0 14 162 10 714.6 0 10 163 11 714.6 0 11 164 16 714.6 0 16 165 18 714.6 0 18 166 15 714.6 0 15 167 17 714.6 0 17 168 30 714.6 0 30 169 29 714.6 0 29 170 22 714.6 0 22 171 17 714.6 0 17 172 23 714.6 0 23 173 18 714.6 0 18 174 11 714.6 0 11 175 15 714.6 0 15 176 21 714.6 0 21 177 14 714.6 0 14 178 10 714.6 0 10 179 19 714.6 0 19 180 22 714.6 0 22 181 19 714.6 0 19 182 24 714.6 0 24 183 20 714.6 0 20 184 17 714.6 0 17 185 25 714.6 0 25 186 19 714.6 0 19 187 13 714.6 0 13 188 18 714.6 0 18 189 28 714.6 0 28 190 26 714.6 0 26 191 15 714.6 0 15 192 19 714.6 0 19 193 34 714.6 0 34 194 28 714.6 0 28 195 91 714.6 0 91 196 85 714.6 0 85 197 47 714.6 0 47 198 21 714.6 0 21 199 21 714.6 0 21 200 19 714.6 0 19 201 14 714.6 0 14 202 7 714.6 0 7 203 11 714.6 0 11 204 17 714.6 0 17 205 16 714.6 0 16 206 16 714.6 0 16 207 20 714.6 0 20 208 11 714.6 0 11 209 22 714.6 0 22 210 17 714.6 0 17 211 10 714.6 0 10 212 25 714.6 0 25 213 38 714.6 0 38 214 40 714.6 0 40 215 39 714.6 0 39 216 30 714.6 0 30 217 22 714.6 0 22 218 43 714.6 0 43 219 39 714.6 0 39 220 28 714.6 0 28 221 21 714.6 0 21 222 15 714.6 0 15 223 13 714.6 0 13 224 23 714.6 0 23 225 23 714.6 0 23 226 16 714.6 0 16 227 27 714.6 0 27 228 19 714.6 0 19 229 34 714.6 0 34 230 31 714.6 0 31 231 22 714.6 0 22 232 12 714.6 0 12 233 16 714.6 0 16 234 25 714.6 0 25 235 34 714.6 0 34 236 85 714.6 0 85 237 35 714.6 0 35 238 61 714.6 0 61 239 47 714.6 0 47 240 47 714.6 0 47 241 20 714.6 0 20 242 23 714.6 0 23 243 11 714.6 0 11 244 37 714.6 0 37 245 38 714.6 0 38 246 25 714.6 0 25 247 49 714.6 0 49 248 20 714.6 0 20 249 9 714.6 0 9 250 14 714.6 0 14 251 13 714.6 0 13 252 14 714.6 0 14 253 10 714.6 0 10 254 22 714.6 0 22 255 26 714.6 0 26 256 40 714.6 0 40 257 35 714.6 0 35 258 19 714.6 0 19 259 20 714.6 0 20 260 121 714.6 0 121 261 25 714.6 0 25 262 23 714.6 0 23 263 22 714.6 0 22 264 12 714.6 0 12 265 19 714.6 0 19 266 18 714.6 0 18 267 22 714.6 0 22 268 21 714.6 0 21 269 13 714.6 0 13 270 9 714.6 0 9 271 12 714.6 0 12 272 17 714.6 0 17 273 12 714.6 0 12 274 18 714.6 0 18 275 26 714.6 0 26 276 23 714.6 0 23 277 14 714.6 0 14 278 22 714.6 0 22 279 46 714.6 0 46 280 22 714.6 0 22 281 18 714.6 0 18 282 15 714.6 0 15 283 28 714.6 0 28 284 33 714.6 0 33 285 26 714.6 0 26 286 48 714.6 0 48 287 159 714.6 0 159 288 97 714.6 0 97 289 21 714.6 0 21 290 25 714.6 0 25 291 12 714.6 0 12 292 21 714.6 0 21 293 46 714.6 0 46 294 44 714.6 0 44 295 20 714.6 0 20 296 36 714.6 0 36 297 35 714.6 0 35 298 201 714.6 0 201 299 23 714.6 0 23 300 18 714.6 0 18 301 50 714.6 0 50 Finished in 1.88 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 35,097 Reads with adapters: 12,678 (36.1%) Reads that were too short: 1 (0.0%) Reads written (passing filters): 12,677 (36.1%) Total basepairs processed: 9,538,919 bp Total written (filtered): 3,445,125 bp (36.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 12678 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 8 0.0 3 0 0 8 19 175 0.0 3 0 167 3 1 4 20 11044 0.0 4 10897 142 2 2 1 21 54 0.0 4 7 47 22 1341 0.0 4 1322 15 2 0 2 23 7 0.0 4 0 1 0 3 3 24 3 0.0 4 0 0 1 0 2 25 2 0.0 4 0 0 0 0 2 74 1 0.0 4 0 0 0 0 1 109 2 0.0 4 0 0 0 2 110 1 0.0 4 0 0 0 1 111 2 0.0 4 0 0 0 2 127 35 0.0 4 0 0 0 0 35 144 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.44 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 12,677 Reads with adapters: 457 (3.6%) Reads written (passing filters): 12,677 (100.0%) Total basepairs processed: 3,445,125 bp Total written (filtered): 3,429,538 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 457 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.7% C: 1.1% G: 87.5% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 198.1 0 1 4 2 49.5 0 2 6 1 3.1 0 1 9 26 0.0 0 1 25 10 173 0.0 1 3 170 11 210 0.0 1 0 210 208 1 0.0 1 1 257 36 0.0 1 34 2 258 2 0.0 1 2 260 3 0.0 1 3 270 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.