This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.58 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 246,650 (8.4%) Reads that were too short: 107,569 (3.7%) Reads written (passing filters): 139,081 (4.8%) Total basepairs processed: 881,011,047 bp Total written (filtered): 28,219,291 bp (3.2%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 246650 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1233 714.6 0 1233 7 32205 714.6 0 32205 8 533 714.6 0 533 9 16188 714.6 0 16188 10 29 714.6 0 29 11 20 714.6 0 20 12 14 714.6 0 14 13 9 714.6 0 9 14 7 714.6 0 7 15 14 714.6 0 14 16 12 714.6 0 12 17 12 714.6 0 12 18 16 714.6 0 16 19 5 714.6 0 5 20 9 714.6 0 9 21 19 714.6 0 19 22 23 714.6 0 23 23 20 714.6 0 20 24 13 714.6 0 13 25 10 714.6 0 10 26 15 714.6 0 15 27 10 714.6 0 10 28 13 714.6 0 13 29 17 714.6 0 17 30 13 714.6 0 13 31 17 714.6 0 17 32 9 714.6 0 9 33 14 714.6 0 14 34 14 714.6 0 14 35 27 714.6 0 27 36 24 714.6 0 24 37 14 714.6 0 14 38 23 714.6 0 23 39 26 714.6 0 26 40 19 714.6 0 19 41 44 714.6 0 44 42 35 714.6 0 35 43 16 714.6 0 16 44 10 714.6 0 10 45 18 714.6 0 18 46 12 714.6 0 12 47 18 714.6 0 18 48 19 714.6 0 19 49 12 714.6 0 12 50 12 714.6 0 12 51 12 714.6 0 12 52 12 714.6 0 12 53 12 714.6 0 12 54 80 714.6 0 80 55 30 714.6 0 30 56 9 714.6 0 9 57 14 714.6 0 14 58 9 714.6 0 9 59 14 714.6 0 14 60 16 714.6 0 16 61 22 714.6 0 22 62 12 714.6 0 12 63 14 714.6 0 14 64 15 714.6 0 15 65 18 714.6 0 18 66 26 714.6 0 26 67 30 714.6 0 30 68 35 714.6 0 35 69 176 714.6 0 176 70 1156 714.6 0 1156 71 258 714.6 0 258 72 17 714.6 0 17 73 27 714.6 0 27 74 19 714.6 0 19 75 86 714.6 0 86 76 85 714.6 0 85 77 76 714.6 0 76 78 91 714.6 0 91 79 62 714.6 0 62 80 235 714.6 0 235 81 386 714.6 0 386 82 190 714.6 0 190 83 159 714.6 0 159 84 253 714.6 0 253 85 307 714.6 0 307 86 263 714.6 0 263 87 271 714.6 0 271 88 552 714.6 0 552 89 613 714.6 0 613 90 424 714.6 0 424 91 392 714.6 0 392 92 152 714.6 0 152 93 312 714.6 0 312 94 266 714.6 0 266 95 179 714.6 0 179 96 175 714.6 0 175 97 206 714.6 0 206 98 157 714.6 0 157 99 200 714.6 0 200 100 852 714.6 0 852 101 1237 714.6 0 1237 102 1860 714.6 0 1860 103 907 714.6 0 907 104 469 714.6 0 469 105 238 714.6 0 238 106 156 714.6 0 156 107 99 714.6 0 99 108 37 714.6 0 37 109 60 714.6 0 60 110 154 714.6 0 154 111 285 714.6 0 285 112 359 714.6 0 359 113 273 714.6 0 273 114 352 714.6 0 352 115 340 714.6 0 340 116 433 714.6 0 433 117 605 714.6 0 605 118 768 714.6 0 768 119 515 714.6 0 515 120 343 714.6 0 343 121 361 714.6 0 361 122 493 714.6 0 493 123 926 714.6 0 926 124 1033 714.6 0 1033 125 542 714.6 0 542 126 352 714.6 0 352 127 326 714.6 0 326 128 306 714.6 0 306 129 349 714.6 0 349 130 499 714.6 0 499 131 920 714.6 0 920 132 1518 714.6 0 1518 133 933 714.6 0 933 134 583 714.6 0 583 135 410 714.6 0 410 136 468 714.6 0 468 137 636 714.6 0 636 138 660 714.6 0 660 139 595 714.6 0 595 140 585 714.6 0 585 141 489 714.6 0 489 142 880 714.6 0 880 143 1342 714.6 0 1342 144 1391 714.6 0 1391 145 804 714.6 0 804 146 537 714.6 0 537 147 647 714.6 0 647 148 844 714.6 0 844 149 871 714.6 0 871 150 795 714.6 0 795 151 889 714.6 0 889 152 692 714.6 0 692 153 648 714.6 0 648 154 740 714.6 0 740 155 602 714.6 0 602 156 864 714.6 0 864 157 1085 714.6 0 1085 158 1297 714.6 0 1297 159 1297 714.6 0 1297 160 1662 714.6 0 1662 161 1318 714.6 0 1318 162 1220 714.6 0 1220 163 917 714.6 0 917 164 782 714.6 0 782 165 641 714.6 0 641 166 507 714.6 0 507 167 721 714.6 0 721 168 1145 714.6 0 1145 169 1237 714.6 0 1237 170 1016 714.6 0 1016 171 979 714.6 0 979 172 776 714.6 0 776 173 552 714.6 0 552 174 530 714.6 0 530 175 462 714.6 0 462 176 1655 714.6 0 1655 177 1726 714.6 0 1726 178 1244 714.6 0 1244 179 1036 714.6 0 1036 180 838 714.6 0 838 181 643 714.6 0 643 182 900 714.6 0 900 183 906 714.6 0 906 184 1068 714.6 0 1068 185 941 714.6 0 941 186 918 714.6 0 918 187 1265 714.6 0 1265 188 1361 714.6 0 1361 189 1771 714.6 0 1771 190 978 714.6 0 978 191 563 714.6 0 563 192 462 714.6 0 462 193 475 714.6 0 475 194 849 714.6 0 849 195 800 714.6 0 800 196 1320 714.6 0 1320 197 899 714.6 0 899 198 730 714.6 0 730 199 1021 714.6 0 1021 200 842 714.6 0 842 201 887 714.6 0 887 202 661 714.6 0 661 203 505 714.6 0 505 204 780 714.6 0 780 205 828 714.6 0 828 206 842 714.6 0 842 207 821 714.6 0 821 208 655 714.6 0 655 209 828 714.6 0 828 210 1105 714.6 0 1105 211 1176 714.6 0 1176 212 1090 714.6 0 1090 213 1206 714.6 0 1206 214 1347 714.6 0 1347 215 1271 714.6 0 1271 216 1267 714.6 0 1267 217 1180 714.6 0 1180 218 1096 714.6 0 1096 219 1004 714.6 0 1004 220 1121 714.6 0 1121 221 998 714.6 0 998 222 1463 714.6 0 1463 223 1377 714.6 0 1377 224 1499 714.6 0 1499 225 1326 714.6 0 1326 226 1108 714.6 0 1108 227 1081 714.6 0 1081 228 1133 714.6 0 1133 229 1012 714.6 0 1012 230 1210 714.6 0 1210 231 817 714.6 0 817 232 795 714.6 0 795 233 876 714.6 0 876 234 1000 714.6 0 1000 235 930 714.6 0 930 236 1078 714.6 0 1078 237 1021 714.6 0 1021 238 1292 714.6 0 1292 239 1159 714.6 0 1159 240 1333 714.6 0 1333 241 1188 714.6 0 1188 242 987 714.6 0 987 243 1031 714.6 0 1031 244 1339 714.6 0 1339 245 1526 714.6 0 1526 246 1326 714.6 0 1326 247 1587 714.6 0 1587 248 1213 714.6 0 1213 249 1676 714.6 0 1676 250 917 714.6 0 917 251 937 714.6 0 937 252 530 714.6 0 530 253 889 714.6 0 889 254 1407 714.6 0 1407 255 1500 714.6 0 1500 256 1307 714.6 0 1307 257 1108 714.6 0 1108 258 1012 714.6 0 1012 259 987 714.6 0 987 260 950 714.6 0 950 261 649 714.6 0 649 262 721 714.6 0 721 263 804 714.6 0 804 264 1317 714.6 0 1317 265 1288 714.6 0 1288 266 1140 714.6 0 1140 267 1054 714.6 0 1054 268 912 714.6 0 912 269 1340 714.6 0 1340 270 1078 714.6 0 1078 271 1139 714.6 0 1139 272 912 714.6 0 912 273 971 714.6 0 971 274 1257 714.6 0 1257 275 1326 714.6 0 1326 276 1446 714.6 0 1446 277 1152 714.6 0 1152 278 796 714.6 0 796 279 746 714.6 0 746 280 1231 714.6 0 1231 281 1355 714.6 0 1355 282 1453 714.6 0 1453 283 1311 714.6 0 1311 284 971 714.6 0 971 285 1023 714.6 0 1023 286 991 714.6 0 991 287 1182 714.6 0 1182 288 1295 714.6 0 1295 289 998 714.6 0 998 290 747 714.6 0 747 291 772 714.6 0 772 292 810 714.6 0 810 293 1037 714.6 0 1037 294 1297 714.6 0 1297 295 1074 714.6 0 1074 296 886 714.6 0 886 297 723 714.6 0 723 298 1045 714.6 0 1045 299 904 714.6 0 904 300 1025 714.6 0 1025 301 683 714.6 0 683 Finished in 5.46 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 139,081 Reads with adapters: 16,809 (12.1%) Reads that were too short: 81 (0.1%) Reads written (passing filters): 16,728 (12.0%) Total basepairs processed: 28,219,291 bp Total written (filtered): 4,590,760 bp (16.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16809 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 11 0.0 3 0 0 7 4 16 157 0.0 3 0 148 7 2 17 16245 0.0 3 15763 417 48 17 18 45 0.0 3 1 40 3 1 19 18 0.0 3 3 4 5 6 20 184 0.0 3 163 6 0 15 22 4 0.0 3 0 0 0 4 23 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 28 4 0.0 3 0 0 0 4 31 1 0.0 3 0 0 0 1 34 7 0.0 3 0 0 1 6 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 6 0.0 3 0 0 0 6 38 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 49 4 0.0 3 0 0 0 4 52 4 0.0 3 0 0 0 4 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 66 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 71 8 0.0 3 0 0 0 8 72 1 0.0 3 0 0 0 1 73 4 0.0 3 0 0 0 4 74 6 0.0 3 0 0 0 6 75 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 88 3 0.0 3 0 0 0 3 89 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 1 1 101 3 0.0 3 0 0 1 2 103 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 186 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 2 0.0 3 0 0 0 2 205 3 0.0 3 0 0 0 3 218 2 0.0 3 0 0 0 2 221 6 0.0 3 0 0 0 6 226 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 280 3 0.0 3 0 0 0 3 281 6 0.0 3 0 0 0 6 282 6 0.0 3 0 0 0 6 283 3 0.0 3 0 0 0 3 284 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 291 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.61 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 16,728 Reads with adapters: 1,330 (8.0%) Reads written (passing filters): 16,728 (100.0%) Total basepairs processed: 4,590,760 bp Total written (filtered): 4,586,762 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1330 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 39.4% C: 17.5% G: 42.0% T: 1.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1322 261.4 0 1322 4 8 65.3 0 8