This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.85 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 121,792 (4.2%) Reads that were too short: 56,325 (1.9%) Reads written (passing filters): 65,467 (2.2%) Total basepairs processed: 881,011,047 bp Total written (filtered): 16,593,598 bp (1.9%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 121792 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 812 714.6 0 812 7 888 714.6 0 888 8 30684 714.6 0 30684 9 11632 714.6 0 11632 10 65 714.6 0 65 11 34 714.6 0 34 12 57 714.6 0 57 13 73 714.6 0 73 14 37 714.6 0 37 15 35 714.6 0 35 16 33 714.6 0 33 17 48 714.6 0 48 18 36 714.6 0 36 19 55 714.6 0 55 20 52 714.6 0 52 21 26 714.6 0 26 22 43 714.6 0 43 23 54 714.6 0 54 24 50 714.6 0 50 25 62 714.6 0 62 26 49 714.6 0 49 27 37 714.6 0 37 28 40 714.6 0 40 29 32 714.6 0 32 30 34 714.6 0 34 31 45 714.6 0 45 32 39 714.6 0 39 33 38 714.6 0 38 34 45 714.6 0 45 35 48 714.6 0 48 36 76 714.6 0 76 37 47 714.6 0 47 38 45 714.6 0 45 39 43 714.6 0 43 40 43 714.6 0 43 41 32 714.6 0 32 42 35 714.6 0 35 43 27 714.6 0 27 44 42 714.6 0 42 45 45 714.6 0 45 46 45 714.6 0 45 47 39 714.6 0 39 48 32 714.6 0 32 49 34 714.6 0 34 50 36 714.6 0 36 51 43 714.6 0 43 52 44 714.6 0 44 53 139 714.6 0 139 54 37 714.6 0 37 55 42 714.6 0 42 56 32 714.6 0 32 57 37 714.6 0 37 58 45 714.6 0 45 59 41 714.6 0 41 60 39 714.6 0 39 61 39 714.6 0 39 62 40 714.6 0 40 63 46 714.6 0 46 64 35 714.6 0 35 65 39 714.6 0 39 66 49 714.6 0 49 67 47 714.6 0 47 68 51 714.6 0 51 69 37 714.6 0 37 70 41 714.6 0 41 71 48 714.6 0 48 72 34 714.6 0 34 73 47 714.6 0 47 74 74 714.6 0 74 75 52 714.6 0 52 76 37 714.6 0 37 77 53 714.6 0 53 78 78 714.6 0 78 79 77 714.6 0 77 80 116 714.6 0 116 81 121 714.6 0 121 82 126 714.6 0 126 83 118 714.6 0 118 84 111 714.6 0 111 85 156 714.6 0 156 86 217 714.6 0 217 87 273 714.6 0 273 88 101 714.6 0 101 89 92 714.6 0 92 90 62 714.6 0 62 91 94 714.6 0 94 92 123 714.6 0 123 93 154 714.6 0 154 94 160 714.6 0 160 95 214 714.6 0 214 96 146 714.6 0 146 97 126 714.6 0 126 98 142 714.6 0 142 99 133 714.6 0 133 100 188 714.6 0 188 101 206 714.6 0 206 102 184 714.6 0 184 103 81 714.6 0 81 104 73 714.6 0 73 105 54 714.6 0 54 106 45 714.6 0 45 107 38 714.6 0 38 108 28 714.6 0 28 109 57 714.6 0 57 110 86 714.6 0 86 111 69 714.6 0 69 112 86 714.6 0 86 113 104 714.6 0 104 114 83 714.6 0 83 115 102 714.6 0 102 116 210 714.6 0 210 117 338 714.6 0 338 118 363 714.6 0 363 119 180 714.6 0 180 120 100 714.6 0 100 121 91 714.6 0 91 122 128 714.6 0 128 123 125 714.6 0 125 124 120 714.6 0 120 125 141 714.6 0 141 126 137 714.6 0 137 127 144 714.6 0 144 128 109 714.6 0 109 129 107 714.6 0 107 130 113 714.6 0 113 131 146 714.6 0 146 132 165 714.6 0 165 133 199 714.6 0 199 134 136 714.6 0 136 135 128 714.6 0 128 136 154 714.6 0 154 137 213 714.6 0 213 138 530 714.6 0 530 139 447 714.6 0 447 140 202 714.6 0 202 141 121 714.6 0 121 142 148 714.6 0 148 143 172 714.6 0 172 144 171 714.6 0 171 145 154 714.6 0 154 146 171 714.6 0 171 147 137 714.6 0 137 148 109 714.6 0 109 149 123 714.6 0 123 150 118 714.6 0 118 151 149 714.6 0 149 152 137 714.6 0 137 153 119 714.6 0 119 154 113 714.6 0 113 155 122 714.6 0 122 156 133 714.6 0 133 157 188 714.6 0 188 158 165 714.6 0 165 159 207 714.6 0 207 160 155 714.6 0 155 161 135 714.6 0 135 162 144 714.6 0 144 163 134 714.6 0 134 164 98 714.6 0 98 165 121 714.6 0 121 166 140 714.6 0 140 167 163 714.6 0 163 168 190 714.6 0 190 169 154 714.6 0 154 170 169 714.6 0 169 171 206 714.6 0 206 172 140 714.6 0 140 173 116 714.6 0 116 174 126 714.6 0 126 175 114 714.6 0 114 176 118 714.6 0 118 177 147 714.6 0 147 178 119 714.6 0 119 179 193 714.6 0 193 180 204 714.6 0 204 181 172 714.6 0 172 182 143 714.6 0 143 183 137 714.6 0 137 184 201 714.6 0 201 185 174 714.6 0 174 186 170 714.6 0 170 187 244 714.6 0 244 188 229 714.6 0 229 189 117 714.6 0 117 190 69 714.6 0 69 191 96 714.6 0 96 192 132 714.6 0 132 193 129 714.6 0 129 194 200 714.6 0 200 195 238 714.6 0 238 196 133 714.6 0 133 197 100 714.6 0 100 198 114 714.6 0 114 199 248 714.6 0 248 200 257 714.6 0 257 201 99 714.6 0 99 202 85 714.6 0 85 203 81 714.6 0 81 204 132 714.6 0 132 205 193 714.6 0 193 206 204 714.6 0 204 207 172 714.6 0 172 208 162 714.6 0 162 209 102 714.6 0 102 210 149 714.6 0 149 211 144 714.6 0 144 212 168 714.6 0 168 213 174 714.6 0 174 214 252 714.6 0 252 215 208 714.6 0 208 216 242 714.6 0 242 217 168 714.6 0 168 218 155 714.6 0 155 219 153 714.6 0 153 220 183 714.6 0 183 221 134 714.6 0 134 222 95 714.6 0 95 223 102 714.6 0 102 224 147 714.6 0 147 225 165 714.6 0 165 226 142 714.6 0 142 227 154 714.6 0 154 228 228 714.6 0 228 229 168 714.6 0 168 230 130 714.6 0 130 231 147 714.6 0 147 232 115 714.6 0 115 233 131 714.6 0 131 234 92 714.6 0 92 235 70 714.6 0 70 236 175 714.6 0 175 237 195 714.6 0 195 238 174 714.6 0 174 239 203 714.6 0 203 240 213 714.6 0 213 241 96 714.6 0 96 242 60 714.6 0 60 243 91 714.6 0 91 244 102 714.6 0 102 245 121 714.6 0 121 246 134 714.6 0 134 247 149 714.6 0 149 248 178 714.6 0 178 249 185 714.6 0 185 250 77 714.6 0 77 251 59 714.6 0 59 252 70 714.6 0 70 253 65 714.6 0 65 254 104 714.6 0 104 255 126 714.6 0 126 256 135 714.6 0 135 257 133 714.6 0 133 258 233 714.6 0 233 259 210 714.6 0 210 260 245 714.6 0 245 261 89 714.6 0 89 262 128 714.6 0 128 263 141 714.6 0 141 264 117 714.6 0 117 265 94 714.6 0 94 266 157 714.6 0 157 267 206 714.6 0 206 268 253 714.6 0 253 269 233 714.6 0 233 270 135 714.6 0 135 271 125 714.6 0 125 272 212 714.6 0 212 273 1403 714.6 0 1403 274 3845 714.6 0 3845 275 3528 714.6 0 3528 276 558 714.6 0 558 277 161 714.6 0 161 278 115 714.6 0 115 279 199 714.6 0 199 280 436 714.6 0 436 281 332 714.6 0 332 282 193 714.6 0 193 283 247 714.6 0 247 284 209 714.6 0 209 285 257 714.6 0 257 286 282 714.6 0 282 287 867 714.6 0 867 288 363 714.6 0 363 289 306 714.6 0 306 290 196 714.6 0 196 291 163 714.6 0 163 292 251 714.6 0 251 293 3086 714.6 0 3086 294 1752 714.6 0 1752 295 5886 714.6 0 5886 296 540 714.6 0 540 297 857 714.6 0 857 298 2753 714.6 0 2753 299 5526 714.6 0 5526 300 8274 714.6 0 8274 301 3170 714.6 0 3170 Finished in 3.14 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 65,467 Reads with adapters: 31,235 (47.7%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 31,219 (47.7%) Total basepairs processed: 16,593,598 bp Total written (filtered): 8,614,309 bp (51.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 31235 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 14 0.0 3 0 0 9 5 16 250 0.0 3 0 219 19 12 17 30748 0.0 3 29387 1139 178 44 18 83 0.0 3 2 75 6 19 71 0.0 3 3 1 40 27 20 24 0.0 3 2 0 0 22 24 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 3 0.0 3 0 0 0 3 34 2 0.0 3 0 0 0 2 37 1 0.0 3 1 41 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 0 3 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 68 1 0.0 3 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 170 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.10 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 31,219 Reads with adapters: 385 (1.2%) Reads written (passing filters): 31,219 (100.0%) Total basepairs processed: 8,614,309 bp Total written (filtered): 8,613,143 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 385 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.1% C: 21.3% G: 17.4% T: 11.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 375 487.8 0 375 4 9 121.9 0 9 5 1 30.5 0 1