This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.94 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,926,947 Reads with adapters: 447,218 (15.3%) Reads that were too short: 200,795 (6.9%) Reads written (passing filters): 246,423 (8.4%) Total basepairs processed: 881,011,047 bp Total written (filtered): 45,916,602 bp (5.2%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 447218 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1111 714.6 0 1111 7 30872 714.6 0 30872 8 448 714.6 0 448 9 21808 714.6 0 21808 10 52 714.6 0 52 11 14 714.6 0 14 12 16 714.6 0 16 13 10 714.6 0 10 14 12 714.6 0 12 15 12 714.6 0 12 16 8 714.6 0 8 17 14 714.6 0 14 18 14 714.6 0 14 19 10 714.6 0 10 20 16 714.6 0 16 21 13 714.6 0 13 22 9 714.6 0 9 23 8 714.6 0 8 24 12 714.6 0 12 25 7 714.6 0 7 26 7 714.6 0 7 27 12 714.6 0 12 28 14 714.6 0 14 29 14 714.6 0 14 30 14 714.6 0 14 31 8 714.6 0 8 32 11 714.6 0 11 33 12 714.6 0 12 34 14 714.6 0 14 35 22 714.6 0 22 36 9 714.6 0 9 37 13 714.6 0 13 38 15 714.6 0 15 39 15 714.6 0 15 40 44 714.6 0 44 41 177 714.6 0 177 42 42 714.6 0 42 43 8 714.6 0 8 44 8 714.6 0 8 45 8 714.6 0 8 46 10 714.6 0 10 47 17 714.6 0 17 48 18 714.6 0 18 49 4 714.6 0 4 50 4 714.6 0 4 51 4 714.6 0 4 52 4 714.6 0 4 53 6 714.6 0 6 54 13 714.6 0 13 55 15 714.6 0 15 56 9 714.6 0 9 57 8 714.6 0 8 58 6 714.6 0 6 59 16 714.6 0 16 60 10 714.6 0 10 61 7 714.6 0 7 62 10 714.6 0 10 63 14 714.6 0 14 64 15 714.6 0 15 65 38 714.6 0 38 66 39 714.6 0 39 67 42 714.6 0 42 68 4 714.6 0 4 69 17 714.6 0 17 70 11 714.6 0 11 71 27 714.6 0 27 72 21 714.6 0 21 73 8 714.6 0 8 74 7 714.6 0 7 75 5 714.6 0 5 76 13 714.6 0 13 77 15 714.6 0 15 78 60 714.6 0 60 79 181 714.6 0 181 80 214 714.6 0 214 81 1237 714.6 0 1237 82 1027 714.6 0 1027 83 1155 714.6 0 1155 84 669 714.6 0 669 85 874 714.6 0 874 86 1937 714.6 0 1937 87 1627 714.6 0 1627 88 1489 714.6 0 1489 89 1119 714.6 0 1119 90 393 714.6 0 393 91 726 714.6 0 726 92 646 714.6 0 646 93 1055 714.6 0 1055 94 1050 714.6 0 1050 95 564 714.6 0 564 96 630 714.6 0 630 97 1644 714.6 0 1644 98 1486 714.6 0 1486 99 1187 714.6 0 1187 100 673 714.6 0 673 101 707 714.6 0 707 102 1894 714.6 0 1894 103 1443 714.6 0 1443 104 872 714.6 0 872 105 1203 714.6 0 1203 106 1057 714.6 0 1057 107 1552 714.6 0 1552 108 1026 714.6 0 1026 109 1612 714.6 0 1612 110 1718 714.6 0 1718 111 1353 714.6 0 1353 112 1155 714.6 0 1155 113 1629 714.6 0 1629 114 1319 714.6 0 1319 115 1115 714.6 0 1115 116 927 714.6 0 927 117 940 714.6 0 940 118 2075 714.6 0 2075 119 2020 714.6 0 2020 120 1304 714.6 0 1304 121 766 714.6 0 766 122 1288 714.6 0 1288 123 1471 714.6 0 1471 124 1574 714.6 0 1574 125 2008 714.6 0 2008 126 2224 714.6 0 2224 127 1940 714.6 0 1940 128 1449 714.6 0 1449 129 1172 714.6 0 1172 130 1191 714.6 0 1191 131 1192 714.6 0 1192 132 1386 714.6 0 1386 133 2901 714.6 0 2901 134 2178 714.6 0 2178 135 1341 714.6 0 1341 136 1215 714.6 0 1215 137 1331 714.6 0 1331 138 2631 714.6 0 2631 139 3102 714.6 0 3102 140 2648 714.6 0 2648 141 1418 714.6 0 1418 142 1001 714.6 0 1001 143 1494 714.6 0 1494 144 2603 714.6 0 2603 145 2268 714.6 0 2268 146 2106 714.6 0 2106 147 1794 714.6 0 1794 148 1243 714.6 0 1243 149 2028 714.6 0 2028 150 2103 714.6 0 2103 151 2703 714.6 0 2703 152 2842 714.6 0 2842 153 1883 714.6 0 1883 154 2129 714.6 0 2129 155 1567 714.6 0 1567 156 2052 714.6 0 2052 157 3018 714.6 0 3018 158 1777 714.6 0 1777 159 2267 714.6 0 2267 160 1807 714.6 0 1807 161 1863 714.6 0 1863 162 1332 714.6 0 1332 163 1442 714.6 0 1442 164 2609 714.6 0 2609 165 2045 714.6 0 2045 166 1671 714.6 0 1671 167 1236 714.6 0 1236 168 1212 714.6 0 1212 169 1116 714.6 0 1116 170 1273 714.6 0 1273 171 1630 714.6 0 1630 172 2700 714.6 0 2700 173 1960 714.6 0 1960 174 1262 714.6 0 1262 175 904 714.6 0 904 176 1091 714.6 0 1091 177 1361 714.6 0 1361 178 1298 714.6 0 1298 179 2275 714.6 0 2275 180 2528 714.6 0 2528 181 2359 714.6 0 2359 182 1436 714.6 0 1436 183 1218 714.6 0 1218 184 1596 714.6 0 1596 185 2095 714.6 0 2095 186 1670 714.6 0 1670 187 1495 714.6 0 1495 188 1660 714.6 0 1660 189 1988 714.6 0 1988 190 2266 714.6 0 2266 191 1627 714.6 0 1627 192 1368 714.6 0 1368 193 1269 714.6 0 1269 194 1299 714.6 0 1299 195 1181 714.6 0 1181 196 1532 714.6 0 1532 197 1687 714.6 0 1687 198 992 714.6 0 992 199 2065 714.6 0 2065 200 1779 714.6 0 1779 201 1988 714.6 0 1988 202 2048 714.6 0 2048 203 1203 714.6 0 1203 204 1283 714.6 0 1283 205 1782 714.6 0 1782 206 2174 714.6 0 2174 207 2207 714.6 0 2207 208 1808 714.6 0 1808 209 1600 714.6 0 1600 210 2062 714.6 0 2062 211 3685 714.6 0 3685 212 6480 714.6 0 6480 213 4621 714.6 0 4621 214 3692 714.6 0 3692 215 1761 714.6 0 1761 216 1483 714.6 0 1483 217 1543 714.6 0 1543 218 1723 714.6 0 1723 219 2121 714.6 0 2121 220 2556 714.6 0 2556 221 1724 714.6 0 1724 222 1628 714.6 0 1628 223 1950 714.6 0 1950 224 2296 714.6 0 2296 225 2025 714.6 0 2025 226 1717 714.6 0 1717 227 1476 714.6 0 1476 228 1411 714.6 0 1411 229 1729 714.6 0 1729 230 1545 714.6 0 1545 231 1680 714.6 0 1680 232 1604 714.6 0 1604 233 1812 714.6 0 1812 234 2017 714.6 0 2017 235 1639 714.6 0 1639 236 1608 714.6 0 1608 237 1603 714.6 0 1603 238 2147 714.6 0 2147 239 2012 714.6 0 2012 240 2207 714.6 0 2207 241 2410 714.6 0 2410 242 2263 714.6 0 2263 243 1981 714.6 0 1981 244 1509 714.6 0 1509 245 2156 714.6 0 2156 246 1534 714.6 0 1534 247 1516 714.6 0 1516 248 910 714.6 0 910 249 1961 714.6 0 1961 250 2210 714.6 0 2210 251 2699 714.6 0 2699 252 2287 714.6 0 2287 253 1142 714.6 0 1142 254 1546 714.6 0 1546 255 1729 714.6 0 1729 256 1765 714.6 0 1765 257 1664 714.6 0 1664 258 1699 714.6 0 1699 259 2001 714.6 0 2001 260 1914 714.6 0 1914 261 2008 714.6 0 2008 262 1952 714.6 0 1952 263 1439 714.6 0 1439 264 1533 714.6 0 1533 265 1726 714.6 0 1726 266 1514 714.6 0 1514 267 2163 714.6 0 2163 268 1428 714.6 0 1428 269 1725 714.6 0 1725 270 2890 714.6 0 2890 271 2629 714.6 0 2629 272 2429 714.6 0 2429 273 1761 714.6 0 1761 274 2107 714.6 0 2107 275 1862 714.6 0 1862 276 1674 714.6 0 1674 277 2470 714.6 0 2470 278 2512 714.6 0 2512 279 1885 714.6 0 1885 280 1947 714.6 0 1947 281 1273 714.6 0 1273 282 2080 714.6 0 2080 283 2307 714.6 0 2307 284 1421 714.6 0 1421 285 1644 714.6 0 1644 286 1728 714.6 0 1728 287 1864 714.6 0 1864 288 2036 714.6 0 2036 289 2172 714.6 0 2172 290 1910 714.6 0 1910 291 1695 714.6 0 1695 292 1879 714.6 0 1879 293 2814 714.6 0 2814 294 3289 714.6 0 3289 295 2141 714.6 0 2141 296 2331 714.6 0 2331 297 2144 714.6 0 2144 298 2068 714.6 0 2068 299 2433 714.6 0 2433 300 2406 714.6 0 2406 301 948 714.6 0 948 Finished in 9.65 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 246,423 Reads with adapters: 31,895 (12.9%) Reads that were too short: 93 (0.0%) Reads written (passing filters): 31,802 (12.9%) Total basepairs processed: 45,916,602 bp Total written (filtered): 8,706,590 bp (19.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 31895 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 6 0.0 3 0 0 6 19 265 0.0 3 0 261 2 1 1 20 31372 0.0 4 30947 401 19 4 1 21 66 0.0 4 7 56 1 0 2 22 6 0.0 4 0 0 5 0 1 23 11 0.0 4 0 0 0 10 1 24 5 0.0 4 0 0 0 0 5 25 2 0.0 4 0 0 0 0 2 27 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 36 1 0.0 4 1 39 1 0.0 4 0 0 0 1 41 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 53 51 0.0 4 0 0 1 31 19 55 2 0.0 4 0 0 0 0 2 57 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 62 2 0.0 4 0 0 0 0 2 64 3 0.0 4 0 0 0 0 3 66 1 0.0 4 0 0 0 0 1 68 2 0.0 4 0 0 0 0 2 69 1 0.0 4 0 0 0 1 70 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 78 2 0.0 4 0 0 0 0 2 79 3 0.0 4 0 0 0 0 3 80 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 88 2 0.0 4 0 0 0 0 2 89 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 0 0 0 1 93 4 0.0 4 0 0 0 0 4 94 2 0.0 4 0 0 0 0 2 96 4 0.0 4 0 0 0 0 4 97 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 99 3 0.0 4 0 0 0 0 3 102 1 0.0 4 0 0 0 0 1 103 2 0.0 4 0 0 0 0 2 104 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 107 3 0.0 4 0 0 0 0 3 108 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 2 0.0 4 0 0 0 1 1 131 2 0.0 4 0 0 0 0 2 132 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 142 2 0.0 4 0 0 0 0 2 143 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 1 228 6 0.0 4 0 0 0 0 6 230 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.12 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 31,802 Reads with adapters: 1,831 (5.8%) Reads written (passing filters): 31,802 (100.0%) Total basepairs processed: 8,706,590 bp Total written (filtered): 8,670,834 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1831 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.0% C: 1.6% G: 94.0% T: 0.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 10 496.9 0 10 4 1 124.2 0 1 10 2 0.0 1 0 2 11 1742 0.0 1 2 1740 12 11 0.0 1 0 11 14 1 0.0 1 0 1 225 9 0.0 1 9 260 1 0.0 1 0 1 261 50 0.0 1 48 2 262 2 0.0 1 2 263 1 0.0 1 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.